<span style="font-family:Verdana;">The present work describes the synthesis of a novel heterocyclic azo dye by </span><span style="font-family:Verdana;">general diazonization of 2...<span style="font-family:Verdana;">The present work describes the synthesis of a novel heterocyclic azo dye by </span><span style="font-family:Verdana;">general diazonization of 2-amino-4,5-dimethylthiazole followed by the di</span><span style="font-family:Verdana;">azo-</span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">coupling of the resulting diazonium ion with 5-methyl-2-(propan-2-</span><span><span style="font-family:Verdana;">yl)phenol to obtain ligand </span><b><span style="font-family:Verdana;">L</span></b><span style="font-family:Verdana;">. This was characterized using Fouri</span></span><span style="font-family:Verdana;">er-transformed infrared and electronic spectrophotometry. Ligand </span><b><span style="font-family:Verdana;">L </span></b><span style="font-family:Verdana;">was further coordinated with five </span><span style="font-family:Verdana;">metal ions, M:L, 1:2 </span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">[</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">M</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">= Cu(II), Mn(II), Zn(II), Ni(II) and Co(II)</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">]</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">. The </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">coordination compounds obtained were characterized by electronic, IR spectrophotometry, magnetic susceptibility and percentage metal analyses. The results obtained suggested that a thiazoylazo dye was obtained as ligand </span><b><span style="font-family:Verdana;">L</span></b><span style="font-family:Verdana;">. It was proposed that two molecules of the solvent coordinated to the metal ion in addition with the ligands to give an octahedral geometry for copper(II), manganese(II) and nickel(II) complexes. On the other hand, square planar </span><span style="font-family:Verdana;">geometry was suggested for zinc(II) and cobalt(II) complexes. The an</span><span style="font-family:Verdana;">ti-infla</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">- </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">mmatory activity of the ligand and coordination compounds was evaluated using four </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;">-based assays viz: </span><span><span style="font-family:Verdana;">xanthine oxidase and lipoxygenase inhibition assay, membrane stability and protein denaturation assay. The synthesized compounds generally exhibited good anti-inflammatory activity in all </span><span style="font-family:Verdana;">the assays carried out. However, the reference standards, in this instance,</span><span style="font-family:Verdana;"> were more effective in the case of xanthine oxidase, lipoxygenase and protein denaturation inhibitory assays. For the membrane stability study, the </span><span><span style="font-family:Verdana;">coordination compounds and ligand </span><b><span style="font-family:Verdana;">L </span></b><span style="font-family:Verdana;">elicited more potent anti-inflammatory</span></span><span style="font-family:Verdana;"> activity than the standard drug.</span></span></span></span></span>展开更多
The health-promoting properties and chemical profiles of 30 Jew’s ear mushroom varieties were investigated. The antioxidant properties were determined by ferric reducing antioxidant power(FRAP), 1,1-diphenyl-2-picryl...The health-promoting properties and chemical profiles of 30 Jew’s ear mushroom varieties were investigated. The antioxidant properties were determined by ferric reducing antioxidant power(FRAP), 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging, 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) free radical scavenging, and metal chelating ability(MCA) assays, while phenolic profiles were determined by total phenol content(TPC) and total flavonoid content(TFC) colorimetric assays. Total carbohydrate, β-glucan, and melanin contents were determined by colorimetric methods. 5’-Nucleotides, vitamin D_(2), ergosterol, and ergothioneine contents were determined by high performance liquid chromatography(HPLC). Anti-inflammation activities of Jew’s ear were evaluated by the colorimetric protease inhibitory method. The results showed that Jew’s ear mushrooms possessed substantial phenolics and antioxidant properties. All the Jew’s ear varieties contain high amount of total carbohydrate, β-glucan, reducing sugar, melanin, pectin, vitamin D2, ergosterol, and ergothioneine. The current findings could provide scientific information for breeders to nurture desired varieties and for food industry to develop new health promoting products.展开更多
A series of mono L-amino acid ester, mono non-steroid anti-inflammation drug (NSAID), carboxylic ester derivatives of acyclonucleoside phosphonates were prepared by using a "one pot synthesis" method and their in ...A series of mono L-amino acid ester, mono non-steroid anti-inflammation drug (NSAID), carboxylic ester derivatives of acyclonucleoside phosphonates were prepared by using a "one pot synthesis" method and their in vitro anti-HBV activity were evaluated in HepG 2.2.15 cells. Compound 9a exhibited more potent anti-HBV activity and lower cytotoxicity than those of adefovir dipivoxil with IC50 and selective index (SI) values of 0.48μmol/L and 763.72, respectively.展开更多
文摘<span style="font-family:Verdana;">The present work describes the synthesis of a novel heterocyclic azo dye by </span><span style="font-family:Verdana;">general diazonization of 2-amino-4,5-dimethylthiazole followed by the di</span><span style="font-family:Verdana;">azo-</span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">coupling of the resulting diazonium ion with 5-methyl-2-(propan-2-</span><span><span style="font-family:Verdana;">yl)phenol to obtain ligand </span><b><span style="font-family:Verdana;">L</span></b><span style="font-family:Verdana;">. This was characterized using Fouri</span></span><span style="font-family:Verdana;">er-transformed infrared and electronic spectrophotometry. Ligand </span><b><span style="font-family:Verdana;">L </span></b><span style="font-family:Verdana;">was further coordinated with five </span><span style="font-family:Verdana;">metal ions, M:L, 1:2 </span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">[</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">M</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">= Cu(II), Mn(II), Zn(II), Ni(II) and Co(II)</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">]</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">. The </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">coordination compounds obtained were characterized by electronic, IR spectrophotometry, magnetic susceptibility and percentage metal analyses. The results obtained suggested that a thiazoylazo dye was obtained as ligand </span><b><span style="font-family:Verdana;">L</span></b><span style="font-family:Verdana;">. It was proposed that two molecules of the solvent coordinated to the metal ion in addition with the ligands to give an octahedral geometry for copper(II), manganese(II) and nickel(II) complexes. On the other hand, square planar </span><span style="font-family:Verdana;">geometry was suggested for zinc(II) and cobalt(II) complexes. The an</span><span style="font-family:Verdana;">ti-infla</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">- </span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">mmatory activity of the ligand and coordination compounds was evaluated using four </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;">-based assays viz: </span><span><span style="font-family:Verdana;">xanthine oxidase and lipoxygenase inhibition assay, membrane stability and protein denaturation assay. The synthesized compounds generally exhibited good anti-inflammatory activity in all </span><span style="font-family:Verdana;">the assays carried out. However, the reference standards, in this instance,</span><span style="font-family:Verdana;"> were more effective in the case of xanthine oxidase, lipoxygenase and protein denaturation inhibitory assays. For the membrane stability study, the </span><span><span style="font-family:Verdana;">coordination compounds and ligand </span><b><span style="font-family:Verdana;">L </span></b><span style="font-family:Verdana;">elicited more potent anti-inflammatory</span></span><span style="font-family:Verdana;"> activity than the standard drug.</span></span></span></span></span>
基金supported by one research grant(R202017)BNU-HKBU United International College,China,and one grant(20200101)+1 种基金The Open Project of National R&D Center for Edible Fungus Processing Technology,Kaifeng,Chinaone grant China Agriculture Research System(No.CARS-20)。
文摘The health-promoting properties and chemical profiles of 30 Jew’s ear mushroom varieties were investigated. The antioxidant properties were determined by ferric reducing antioxidant power(FRAP), 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging, 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) free radical scavenging, and metal chelating ability(MCA) assays, while phenolic profiles were determined by total phenol content(TPC) and total flavonoid content(TFC) colorimetric assays. Total carbohydrate, β-glucan, and melanin contents were determined by colorimetric methods. 5’-Nucleotides, vitamin D_(2), ergosterol, and ergothioneine contents were determined by high performance liquid chromatography(HPLC). Anti-inflammation activities of Jew’s ear were evaluated by the colorimetric protease inhibitory method. The results showed that Jew’s ear mushrooms possessed substantial phenolics and antioxidant properties. All the Jew’s ear varieties contain high amount of total carbohydrate, β-glucan, reducing sugar, melanin, pectin, vitamin D2, ergosterol, and ergothioneine. The current findings could provide scientific information for breeders to nurture desired varieties and for food industry to develop new health promoting products.
基金supported by the National Natural Science Foundation of China(Nos.20962004,81260473)Projects of Guizhou Science and Technology Department(Nos.2013-3031,2012-3013)Excellent Youth Scientific Talents Foundation of Guizhou(No.2013-45)
文摘A series of mono L-amino acid ester, mono non-steroid anti-inflammation drug (NSAID), carboxylic ester derivatives of acyclonucleoside phosphonates were prepared by using a "one pot synthesis" method and their in vitro anti-HBV activity were evaluated in HepG 2.2.15 cells. Compound 9a exhibited more potent anti-HBV activity and lower cytotoxicity than those of adefovir dipivoxil with IC50 and selective index (SI) values of 0.48μmol/L and 763.72, respectively.