No-touch isolation technique in emergency pancreaticoduodenectomy for neoplastic hemorrhage: Two case reports and review of literature

BACKGROUND Emergency pancreaticoduodenectomy(EPD)is a rare event for complex periam-pullary etiology.Increased intraoperative blood loss is correlated with poor post-operative outcomes.CASE SUMMARY Two patients underwent EPD using a no-touch isolation technique,in which all arteries supplying the pancreatic head region were ligated and divided before manipulation of the pancreatic head and duodenum.The operative times were 220 and 239 min,and the blood loss was 70 and 270 g,respectively.The patients were discharged on the 14^(th) and 10^(th) postoperative day,respectively.Thirty-two patients underwent EPD for the treatment of neoplastic bleeding.The mean operative time was 361.6 min,and the mean blood loss was 747.3 g.The comp-lication rate was 37.5%.The in-hospital mortality rate was 9.38%.CONCLUSION The no-touch isolation technique is feasible,safe,and effective for reducing intraoperative blood loss in EPD.

Non-enzymatic methods for isolation of stromal vascular fraction and adipose-derived stem cells:A systematic review

BACKGROUND Adipose-derived stem cells(ADSCs)and the stromal vascular fraction(SVF)have garnered substantial interest in regenerative medicine due to their potential to treat a wide range of conditions.Traditional enzymatic methods for isolating these cells face challenges such as high costs,lengthy processing time,and regulatory complexities.AIM This systematic review aimed to assess the efficacy and practicality of nonenzymatic,mechanical methods for isolating SVF and ADSCs,comparing these to conventional enzymatic approaches.METHODS Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines,a comprehensive literature search was conducted across multiple databases.Studies were selected based on inclusion criteria focused on non-enzymatic isolation methods for SVF and ADSCs from adipose tissue.The risk of bias was assessed,and a qualitative synthesis of findings was performed due to the methodological heterogeneity of the included studies.RESULTS Nineteen studies met the inclusion criteria,highlighting various mechanical techniques such as centrifugation,vortexing,and ultrasonic cavitation.The review identified significant variability in cell yield and viability,and the integrity of isolated cells across different non-enzymatic methods compared to enzymatic procedures.Despite some advantages of mechanical methods,including reduced processing time and avoidance of enzymatic reagents,the evidence suggests a need for optimization to match the cell quality and therapeutic efficacy achievable with enzymatic isolation.CONCLUSION Non-enzymatic,mechanical methods offer a promising alternative to enzymatic isolation of SVF and ADSCs,potentially simplifying the isolation process and reducing regulatory hurdles.However,further research is necessary to standardize these techniques and ensure consistent,high-quality cell yields for clinical applications.The development of efficient,safe,and reproducible non-enzymatic isolation methods could significantly advance the field of regenerative medicine.

An effective technique for isolating adult activated Schwann cells

BACKGROUND： Schwann cells （SCs） are neuroglial cells of peripheral nerve and play a key role in repairing peripheral nerve injury; therefore, it provides an important evidence for transplantation of SCs which are characterized by active proliferation and adult high-purity in vitro after nerve injury in clinic, and also develops a new therapeutic way for nerve injury.OBJECTEVE： To investigate an effective technique for isolating adult activated Schwann cells,DESIGN： Controlled observational study.SETTING： Mudanjiang Medical College.MATERIALS： The experiment was completed at the Department of Medical Genetics of Harbin Medical University from March 2003 to April 2005. Health female Wistar rats, aged 2 months, weighting 150-160 g, were randomly divided into 3 groups with 5 in each group.METHODS： The right sciatic nerves from 15 Wistar rats were exposed and transected at the mid thigh under pentobarbital anesthesia （4 mg/kg, Lp）. Seven days later, the distal segments of the predegenerated nerves were removed and used to produce adult Schwann cell cultures. The distal segment of the predegenerated nerve, 20 mm in length, was resected. The nerve was cut into pieces 1 mm in length and incubated for 3 hours under CO2 at 37 ℃ with an enzyme mixture of 0.05% collagenase/dispase. Rats were divided into 3 groups： ① Group 1： The nerve fragments were explanted in poly-L-lysine and laminin-coated dishes with BS medium from the 1st to the 6th day, On the 6^th day, the fragments were removed into a new poly-L-lysine-laminin-coated dish and the BS medium was changed to BS with 10% FBS, The nerve fragments were replaced repeatedly in the same way in new dishes on the 12^th and the 18th days. ② Group 2： For the first 3 days, the nerve fragments were fed with BS with 10% FBS. This medium was changed to BS medium on the third day. The nerve fragments were removed to another dish on day 6 and BS medium was changed to BS with 25 mI.JL FBS. Hereafter the culture method was the same as for group 1. ③ Group 3： For the first 6 days, nerve fragments were incubated in a dish not coated with poly-L-lysine and laminin, in BS medium supplemented with 8×10^7 U/L of penicillin-streptomycin. On the 6th day, the nerve fragments were removed to a poly-L-lysine-laminin-coated dish and cultured in BS with 25 mI.JL FBS, On the 12th day, the nerve fragments were explanted a second dish and fed with BS containing 100 mL/L FBS. On the 18^th day, they were explanted to a third poly-L-lysine-laminin-coated dish, SCs were obtained from all 3 dishes on the 21st day, Finally, purity and density of SCs were identified and proliferation index was calculated at the same time.MAIN OUTCOME MEASURES ： Purity and density of SCs cultured with various methods in the three groups for 21 days.RESULTS ： ① Isolation and proliferation of SCs： In the group 1, they increased in number after 4 days and both purity and density of cultured SCs were significantly higher than those from group 2. In the group 2, there were few fibroblasts. In the group 3, both purity and density of cultured SCs were remarkably higher than in those from groups 1 or 2. Then optimal proliferation was soon seen and the rapid expansion of SC populations suppressed the development of contaminating fibroblasts. On the 21st day, SCs proliferated to achieve maximal density and were too crowded to be counted. With Chi-square test, the data of the purity and the density were analyzed from groups 1 to 3, the result indicated X^2=430.47, P 〈 0.05. ② Characterization and proliferation rate of SCs： Immunostaining for S100 protein was evident in the cell soma and the processes of all three groups in cultures of SCs. SCs in vitro demonstrated typical bior tri-polar morphology, had oval nuclei, and stained brightly for $100. The proliferation rate of SCs was assessed with double fluorescence staining for BrdU and S100 on the 21^st day of all three groups in cultures. About 40%-50% of the total SCs in the each group showed BrdU incorporation.CONCLUSION： The method is to use predegeneration in vivo, differential speed culture supplemented with the penicillin-streptomycin in low concentration, and changing of the concentration of FBS in the BS medium from 0 to 100 mL/L. This method allows remarkable suppression of fibroblast growth and attainment of SC proliferation and purity, in a short time, from adult nerves.

Isolation of protoplast from callus of Populus euphratica and H^+ fluxes across plasma membrane under NaCl stress

We used callus of Populus euphratica Olive to isolate protoplasts, and IT fluxes across plasma membrane were investigated. The concentration of enzymes for protoplast isolation, e.g. cellulase, pectolyase, macerozyme, hemicellulase, and sorbitol content, incubation time were systemically studied. High yield and viability of protoplast was achieved after 6-8 hours incubation of P. euphratica callus in enzyme solution containing 1.5% （w：v） cellulase R-10, 0.1% （w：v） pectolyase Y-23, 0.2% （w：v） macerozyme R-10, 0.05% （w：v） hemicellulase and 0.75M）.80 mol·L^-1 sorbitol. Non-invasively ion selective microelectrode technique was used to access proton fluxes in the absence and presence of NaCl （20 mmol.L-1）. Salt-induced transient net IT effiux was observed in the plasma membrane ofP. euphratica cells. The shift of IT flux response to NaC1 shock and the relevance to salt tolerance were discussed.

Alternative technique to save ischemic bowel segment in management of neonatal short bowel syndrome: A case report

BACKGROUND Congenital short bowel syndrome(SBS)associated with malrotation,gut volvulus and jejuno-ileal atresia is a very rare condition.It is a severe challenge for surgeons to preserve residual ischemic bowel segment in the management of short bowel syndrome,especially in neonates.CASE SUMMARY We report a newborn baby with gut malrotation associated with jejuno-ileal atresia,congenital SBS and jejunal volvulus.Hematemesis and abdominal distention were noted.At laparotomy,malrotation associated with jejuno-ileal atresia,congenital SBS and jenunal volvulus was confirmed.The total length of the small bowel was 63 cm with proximal jejunal bowel segment measuring 38 cm,including 18 cm necrotic segment below the Treitz’s ligament and 20 cm severe ischemic segment.The distal part of the small bowel was 25 cm in length and only about 0.8 cm in diameter.Ladd’s procedure,necrotic segment resection and end-to-back duodeno-ileal anastomosis were performed.The residual severe ischemic jejunum was preserved with single proximal stoma and distal end closure.Three months later,to restore the continuity of the isolated gut segment,end-to-end duodeno-jejunal and jejuno-ileal anastomosis was performed.The entire functional small bowel length increased to 80 cm.Intravenous fluid therapy and parenteral nutrition were discontinued on the 10th day postoperatively.Twelve months later,her body weight was 9.5 kg.CONCLUSION Isolation of severe ischemic bowel segment and staged anastomosis to restore the gut continuity for infants with SBS are safe and feasible.

Robust Fault Detection and Isolation Based on Finite-frequency H__/H_∞ Unknown Input Observers and Zonotopic Threshold Analysis

This work proposes a robust fault detection and isolation scheme for discrete-time systems subject to actuator faults,in which a bank of H_/H∞ fault detection unknown input observers(UIOs) and a zonotopic threshold analysis strategy are considered. In observer design, finite-frequency H_ index based on the generalized Kalman-Yakubovich-Popov lemma and H∞ technique are utilized to evaluate worst-case fault sensitivity and disturbance attenuation performance, respectively. The proposed H_/H∞ fault detection observers are designed to be insensitive to the corresponding actuator fault only, but sensitive to others.Then, to overcome the weakness of predefining threshold for FDI decision-making, this work proposes a zonotopic threshold analysis method to evaluate the generated residuals. The FDI decision-making relies on the evaluation with a dynamical zonotopic threshold. Finally, numerical simulations are provided to show the feasibility of the proposed scheme.

Separation, Identification, Isolation and Characterization of Degradation Product of Osimertinib Tablets by UPLC-QTOF-MS/MS and NMR: Evaluation of <i>In-Silico</i>Safety Assessment for Osimertinib (OSM) and Degradation Product (DP)

The present work encompasses identification and characterization of major degradation product (DP) of OSM observed in base hydrolytic stress study. The separation of DP was carried out on a non-polar stationary phase by using high-performance liquid chromatography system (HPLC). Using waters X-bridge (250 mm × 4.6 mm, 5 μm) C18 column with gradient elution program. For the characterization study, stress samples were subjected to HPLC and UPLC-QTOF-MS/MS and based on mass fragmentation pattern, plausible structure was deduced. Further, the DP was isolated using semi-prepara- tive liquid chromatography and concentrated the fractions using lyophilization. The isolated DP was subjected to extensive 1D (1H, 13C, and DEPT-135) and 2D (COSY, HSQC and HMBC) nuclear magnetic resonance (NMR) studies to authenticate the structure. The impurity was unambiguously named as N-(2-((2-(dimethylamino)ethyl)(methyl)amino)-4-metho-xy-5-((4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)-3-methoxypropanamide. Add- itionally, the In-Silico structure activity relation (QSAR) assessed through statistical based software’s DEREK NexusTM, and MultiCASE, Case UltraTM widely accepted and respected software’s for DP and OSM.

氟化物磁光晶体的研究进展与应用

光通信技术发展迅速,激光平均功率不断提高,磁光隔离器是保证激光单向传输、维持光学系统稳定高效工作的基础,而磁光介质作为磁光隔离器的核心部件也因此得以快速发展。首先简要介绍了几种磁光材料以及当前广泛应用的石榴石磁光晶体的缺点、法拉第效应的原理和应用,随后系统分析了作为新一代磁光晶体的各类氟化物磁光晶体的优缺点,最后展望了CeF_(3)磁光晶体潜在的应用前景以及未来氟化物磁光晶体的发展趋势。

内镜医师内镜黏膜下剥离术教学的实践与思考

内镜黏膜下剥离术(endoscopic submucosal dissection,ESD)是目前消化道早癌的首选微创治疗方法,其操作难度大,是国内内镜教学培训的热点和难点。本研究以重庆医科大学附属第一医院青年医师和进修ESD操作的内镜医师为教学对象,建立了ESD规范化培训流程。培训周期共3月,教学内容包括理论培训、临床观摩、简易离体猪胃模型操作、真实患者实践操作,以及结业考核。通过问卷调查,100%的学员认为重庆医科大学附属第一医院内镜中心ESD教学培训对于掌握ESD技术是非常必要的,且能明显提高学员的ESD操作水平。因此,重庆医科大学附属第一医院内镜中心ESD规范化培训流程总体设置合理,为我国内镜医师的教学与培训提供一定的借鉴与参考。

隔震支座在舟曲县峰迭新区第二幼儿园项目中的应用及施工特点分析

随着城市建设的不断发展,幼儿园项目作为基础教育设施的一部分,其建设也面临着新的挑战。论文围绕舟曲县峰迭新区第二幼儿园建设项目展开研究,重点关注隔震支座在项目中的应用。该项目采用框架结构,工程特点主要表现在施工难点上,包括多工序、交叉作业、室外管网连接、场地有限等方面。论文将深入分析工程特点,探讨施工中的难点,并提出解决方案,以确保工程的高效顺利进行,为抗震建筑和类似工程提供有益的经验和教训。

橡皮障隔离技术在椅旁CAD/CAM瓷嵌体修复治疗中的应用

目的研究橡皮障隔离技术在椅旁计算机辅助设计(CAD)/计算机辅助制作(CAM)瓷嵌体修复治疗中的应用效果。方法选择2020年1月—2022年1月医院牙体牙髓科行椅旁CAD/CAM瓷嵌体修复治疗的患者60例,随机分为研究组和对照组,各30例。对照组按照常规流程操作,使用常规方法进行术野隔离;研究组在操作治疗中运用橡皮障隔离技术。比较两组总治疗时间和不良反应发生情况。结果两组患者的嵌体冠修复体在冠边缘、密合性、咬合关系等方面均良好。研究组总治疗时间(118.83±19.15)min,较对照组(141.26±11.74)min缩短,差异有统计学意义(P<0.05);研究组无不良反应发生,对照组发生1(3.33%)例不良反应。结论橡皮障隔离技术可有效缩短椅旁CAD/CAM瓷嵌体修复治疗时间。

A Compact Self-Isolated MIMO Antenna System for 5G Mobile Terminals

A compact self-isolated Multi Input Multi Output (MIMO) antennaarray is presented for 5G mobile phone devices. The proposed antenna systemis operating at the 3.5 GHz band (3400–3600 MHz) and consists of eight antennaelements placed along two side edges of a mobile device, which meets the currenttrend requirements of full-screen smartphone devices. Each antenna element isdivided into two parts, a front part and back part. The front part consists of anI-shaped feeding line and a modified Hilbert fractal monopole antenna, whereasthe back part is an L-shaped element shorted to the system ground by a0.5 mm short stub. A desirable compactness can be obtained by utilizing the Hilbert space-filling property where the antenna element’s overall planar size printedon the side-edge frame is just (9.57 mm × 5.99 mm). The proposed MIMO antenna system has been simulated, analyzed, fabricated and tested. Based on the selfisolated property, good isolation (better than 15 dB) is attained without employingadditional decoupling elements and/or isolation techniques, which increases system complexity and reduces the antenna efficiency. The scattering parameters,antenna efficiencies, antenna gains, and antenna radiation characteristics areinvestigated to assess the proposed antenna performance. For evaluating the proposed antenna array system performance, the Envelope Correlation Coefficients(ECCs), Mean Effective Gains (MEGs) and channel capacity are calculated.Desirable antenna and MIMO performances are evaluated to confirm the suitability of the proposed MIMO antenna system for 5G mobile terminals.

橡皮障隔离技术联合四手操作法对于提高牙髓炎根管治疗效果的研究

目的探讨橡皮障隔离技术联合四手操作在牙髓炎根管治疗中的疗效。方法选取2021年1月至2022年12月接受根管治疗的牙髓炎患者进行单中心回顾性对照研究。将2022年1月前采用常规隔离技术(棉卷+大容量抽吸)行根管治疗的患者作为对照组,将2022年1月及之后采用橡皮障隔离技术的患者作为观察组。比较2组的根管治疗时间、根管治疗成功率及患者的治疗经验,以总治疗时间、治疗成功率和满意率作为主要指标。本研究资料采用独立样本t检验和χ^(2)检验进行统计分析。结果共有84例成年牙髓炎患者[男50例,女34例,平均年龄为(40.70±12.55)岁]入组。观察组的总治疗时间较对照组短[(45.29±4.77)min vs.(51.36±5.54)min,P<0.001]。观察组的根管治疗成功率显著高于对照组(97.62%vs.83.33%,P<0.05)。观察组患者的满意率显著高于对照组(97.62%vs.80.95%,P<0.05)。结论橡皮障隔离技术联合四手操作可有效缩短牙髓炎患者的根管治疗时间,降低感染风险,提高治疗成功率和患者满意度。

超声引导射频消融在较大乳腺纤维腺瘤治疗中的应用

目的探讨超声引导夹心水隔离技术辅助的射频消融(RFA)在较大乳腺纤维腺瘤(直径≥2 cm)治疗中的应用价值。方法选择2014年12月至2021年3月上海交通大学医学院附属仁济医院治疗的乳腺纤维腺瘤患者39例。患者均接受超声引导夹心水隔离技术辅助的RFA治疗,观察消融前、消融后即刻、消融后1个月、消融后3个月、消融后6个月肿瘤体积变化以及肿瘤体积缩小率,RFA后并发症发生情况。结果39例患者(42个肿瘤)的RFA治疗时间为(176.7±116.6)s。治疗前肿瘤最大直径为2.0~3.4 cm,消融前、消融后即刻、消融后1个月、消融后3个月、消融后6个月肿瘤体积分别为(3.16±2.08)mL、(2.19±1.42)mL、(1.24±0.93)mL、(0.64±0.59)mL、(0.26±0.31)mL,治疗后1、3、6个月超声检查显示肿瘤体积较消融前逐渐缩小,体积缩小率分别为(48.3±17.6)%、(77.8±17.1)%、(84.6±17.4)%。治疗后12个月36个肿瘤消融区消失,6个肿瘤仍可见消融区痕迹。39例患者均无严重治疗相关并发症发生。结论超声引导夹心水隔离技术辅助的RFA治疗直径≥2 cm的乳腺纤维腺瘤安全、微创、有效,适用于无法接受手术治疗的较大乳腺纤维腺瘤患者。

核酸适配体筛选与亲和力评价技术主要研究方向、进展与挑战

核酸适配体是一类具有特异性分子识别能力的单链DNA或者RNA分子,通过指数富集的配体系统进化技术(SELEX)筛选得到。核酸适配体相比抗体具有热稳定性高、便于化学合成与修饰、免疫原性低等优点,在生物分析、生物医学、生物技术等众多领域引起广泛关注。高质量的核酸适配体是应用的基础,然而目前能够满足实际应用的核酸适配体数量还非常有限。如何获得高亲和力、高特异性、高体内稳定性的核酸适配体是核酸适配体领域的技术瓶颈。本文首先简单介绍了SELEX技术的基本原理和核酸库的设计、筛选过程监控、次级文库制备、测序和候选适配体筛选等关键步骤。接着归纳总结了30多年来核酸适配体筛选技术的6个主要研究方向、研究进展和局限性。这6个主要研究方向分别是提高适配体特异性的筛选方法、提高适配体稳定性(抗核酸酶降解能力)的筛选方法、快速筛选方法、复杂靶标适配体筛选方法、小分子靶标适配体筛选方法、提高适配体亲和力的筛选方法。其中快速筛选技术是长期以来持续关注的研究方向,几乎所有物理分离手段都已用于提高SELEX的筛选效率。最近,高效化学反应与SELEX技术的结合为核酸适配体的快速筛选提供了新的策略。本文随后对适合小分子靶标核酸适配体筛选的3类方法进展和存在的问题进行了重点评述。这3类方法分别是基于靶标固定的筛选技术、基于文库固定的筛选技术(捕获-SELEX,Capture-SELEX)和均相筛选技术(氧化石墨烯-SELEX,GO-SELEX)。基于靶标固定的筛选技术尽管存在空间位阻等众多问题,由于其操作的简单性,目前依然应用广泛。近年来Capture-SELEX应用广泛。结合36种靶标适配体的筛选实验条件(文库设计、正筛靶标浓度、负筛靶标的选择和浓度)和所获得的适配体的亲和力(KD,解离常数,dissociation constant)和特异性,对Capture-SELEX的实验条件与适配体性能的关系进行了讨论。统计数据表明,降低正筛靶标浓度有利于提高适配体的亲和力,但不是必要条件。负筛选是目前提高适配体特异性的主要技术手段,但适配体的特异性还不能满足实际需求。负筛选靶标及其浓度的选择差异很大,而且36种靶标中有20种靶标的适配体筛选没有进行负筛选。如何提高核酸适配体的特异性是目前小分子靶标核酸适配体所面临的难题,急需寻找新的策略。本文还列表归纳了近三年利用GO-SELEX进行的13种小分子靶标的实验条件和所获得的适配体的KD和特异性。统计数据表明,GO-SELEX比Capture-SELEX所需要的筛选轮数少,两种方法所获得的适配体的亲和力多在纳摩尔每升水平。Capture-SELEX相对较低的筛选效率应该主要由于文库的自解离问题。核酸适配体的亲和力评价是候选核酸适配体结构与性能评价的重要组成部分。常用的核酸适配体亲和力评价技术包括基于分离、基于固定、均相体系三大类十多种方法。假阳性和假阴性是各种评价技术都有可能存在的问题。本文以纳米金比色法和等温热滴定技术为例评述技术进展,讨论导致不同亲和力评价技术结果不一致性问题的根本原因。本文最后对核酸适配体筛选技术、亲和力评价技术和技术的标准化的未来发展趋势进行了展望。

2023年土耳其强震作用下不同结构模型的抗震性能及地震损伤分析

2023年2月6日土耳其发生M_(W)7.8地震和M_(W)7.5地震,造成土耳其多个城市出现严重人员伤亡和建筑物倒塌。为判断按照中国建筑抗震设计规范设防的多层混凝土建筑结构在本次地震作用下的破坏情况,对中国8度设防区某4层混凝土结构办公楼,采用大型通用有限元软件SAUSG2022,输入土耳其地震3123台站地震动记录,分别对框架结构、框剪结构、隔震结构和减震结构4种结构模型进行弹塑性动力时程分析。结果表明:框架结构、框剪结构和隔震结构在地震动作用下损伤严重,按变形标准判断有倒塌风险;采用黏滞阻尼器减震技术的结构虽然也有一定程度的损伤,但损伤程度有限,建筑物无倒塌风险。

基于手语表达内容与表达特征的手语识别技术综述

手语识别(SLR)技术是打破听障人群与健听人群间交流壁垒的重要技术手段。该文综述了近几年的手语数据集、评价指标以及手语识别方法。首先,系统梳理了手语数据集并分析了手语识别方法的数据集发展方向。其次,详细介绍了手语识别方法的评价指标。然后,根据手语表达内容、手语识别方法所采用的特征分类总结分析了孤立词手语识别方法与连续语句识别方法、仅依靠手部特征的手语识别方法与多特征融合的手语识别方法。最后探讨了手语识别技术面临的挑战及其发展方向。

高烈度区某天燃气工程指挥部隔震加固设计与施工

针对新疆某天燃气工程指挥部的抗震问题,对其采用隔震技术进行加固,并采用“抱柱托梁加垫”和“夹墙梁”的方法进行施工,确保其上部结构安全。采用国际通用的三维空间有限元软件ETABS对其进行建模分析,并用时程分析方法对其进行隔震加固设计分析。在此基础上,分析了其减震效果,并介绍了本工程采用的施工工艺及相应的关键技术。结果表明:该工程隔震加固效果比较显著,能较好地满足现行规范和使用功能的要求,故对其进行隔震加固设计与施工是可行的。

计算机纹理分析技术在鉴别孤立性肺结节多层螺旋CT图像中的临床应用价值

目的探讨计算机纹理分析技术在鉴别孤立性肺结节多层螺旋CT图像中的应用价值,以期为临床早期诊断、制定干预方案提供依据。方法选取2019年8月至2021年8月在南阳南石医院就诊的48例恶性孤立性肺结节患者作为恶性组,另选取50例同期就诊的良性孤立性肺结节患者作为良性组,入院后均行CT检查,并采用计算机纹理技术分析CT图像,比较两组患者CT图像特征,以及平扫期、增强扫描动脉期、增强扫描静脉期CT值及纹理参数,采用Logistic回归方程分析平扫期纹理参数与孤立性肺结节良恶性相关性,采用受试者工作特征曲线(ROC)及曲线下面积(AUC)分析各纹理参数对孤立性肺结节良恶的诊断价值。结果与良性组比较,恶性组患者的平扫期、动脉期及门静脉期错判率较低,差异均有统计学意义(P<0.05);与良性组平扫期熵(1.51±0.06)、相关度(0.23±0.08)、对比度(12.28±2.85)比较,恶性组熵(1.62±0.08)较大,相关度(0.07±0.02)、对比度(8.54±1.36)较低,差异均有统计学意义(P<0.05);经Logistic回归方程分析结果显示,平扫期CT纹理参数熵、相关度、对比度与孤立性肺结节良恶性具有相关性(OR=7.731、0.532、0.463,P>0.05);平扫期纹理参数熵、相关度、对比度对孤立性肺结节良恶性诊断的AUC分别为0.795、0.832、0.856,各参数联合诊断AUC为0.927,优于单一参数诊断。结论螺旋CT图像纹理分析技术用于孤立性肺结节的定性诊断,可为临床早期诊断、针对性制定干预方案提供可靠依据。

医院隔离技术标准WS/T 311-2023

前言本标准为推荐性标准。本标准替代WS/T 311—2009《医院隔离技术规范》,与WS/T 311—2009相比,主要修改内容如下:——在“规范性引用文件”中增加了需引用的标准(见第2章);——在“术语和定义”中增加了“一次性使用医用口罩”、“呼吸道卫生/咳嗽礼仪”、“隔离患者”、“适合性检验”、“佩戴气密性检查”(见3.8.3、3.16、3.17、3.18、3.19),修改了“标准预防”、“医用外科口罩”、“医用防护口罩”、“护目镜”、“防护面罩(防护面屏)”、“隔离衣”、“防护服”、“负压病区(室)”的定义(见3.4、3.8.1、3.8.2、3.8.4、3.8.5、3.8.7、3.8.8、3.15,2009年版的3.4、3.9.2、3.9.3、3.9.4、3.9.5、3.9.7、3.9.8、3.16),删除了“感染链”、“纱布口罩”、“床单位消毒”、“终末消毒”的定义(见2009年版的3.8、3.9.1、3.17、3.18)。