目的:探讨壬苯醇醚和聚维酮碘合剂即复方壬苯醇醚体外协同杀菌效果。方法:将壬苯醇醚、聚维酮碘及二者混合制的复方壬苯醇醚分别对白色念珠菌和淋病奈瑟菌进行最小杀菌浓度(MBC)测定和最短杀菌时间(MBT)测定。结果:壬苯醇醚单独使用未...目的:探讨壬苯醇醚和聚维酮碘合剂即复方壬苯醇醚体外协同杀菌效果。方法:将壬苯醇醚、聚维酮碘及二者混合制的复方壬苯醇醚分别对白色念珠菌和淋病奈瑟菌进行最小杀菌浓度(MBC)测定和最短杀菌时间(MBT)测定。结果:壬苯醇醚单独使用未见杀菌作用,聚维酮碘与复方壬苯醇醚杀菌时间都是2 m in,但单独使用聚维酮碘的MBC要高于复方壬苯醇醚的MBC。结论:壬苯醇醚和聚维酮碘合剂即复方壬苯醇醚有协同杀菌作用且效果优于两者单用。展开更多
The purpose of this study is to evaluate the in vivo retention capabilities of poloxamer-based in situ hydrogels for vaginal application with nonoxinol-9 as the model drug. Two in situ hydrogel formulations, which con...The purpose of this study is to evaluate the in vivo retention capabilities of poloxamer-based in situ hydrogels for vaginal application with nonoxinol-9 as the model drug. Two in situ hydrogel formulations, which contained 18% poloxamer 407 plus 1% poloxamer 188(GEL1, relative hydrophobic)or 6% poloxamer 188(GEL2, relative hydrophilic), were compared with respect to the rheological properties, in vitro hydrogel erosion and drug release. The vaginal retention capabilities of these hydrogel formulations were further determined in two small animal models, including drug quantitation of vaginal rinsing fluid in mice and isotope tracing with99 m Tc in rats. The two formulations exhibited similar phase transition temperatures ranging from 27 to 32 1C. Increasing the content of poloxamer 188 resulted in higher rheological moduli under body temperature, but slightly accelerated hydrogel erosion and drug release. When compared in vivo, GEL1 was eliminated significantly slower in rat vagina than GEL2,while the vaginal retention of these two hydrogel formulations behaved similarly in mice. In conclusion,increases in the hydrophilic content of formulations led to faster hydrogel erosion, drug release andintravaginal elimination. Rats appear to be a better animal model than mice to evaluate the in situ hydrogel for vaginal application.展开更多
文摘目的:探讨壬苯醇醚和聚维酮碘合剂即复方壬苯醇醚体外协同杀菌效果。方法:将壬苯醇醚、聚维酮碘及二者混合制的复方壬苯醇醚分别对白色念珠菌和淋病奈瑟菌进行最小杀菌浓度(MBC)测定和最短杀菌时间(MBT)测定。结果:壬苯醇醚单独使用未见杀菌作用,聚维酮碘与复方壬苯醇醚杀菌时间都是2 m in,但单独使用聚维酮碘的MBC要高于复方壬苯醇醚的MBC。结论:壬苯醇醚和聚维酮碘合剂即复方壬苯醇醚有协同杀菌作用且效果优于两者单用。
基金supported by the National Key Technology R&D Program(2012BAI31B04)the Open Project Program of National Population+1 种基金Family Planning Key Laboratory of Contraceptives Drugs and Devices(2016KF08)the National Natural Science Foundation of China(81102385)
文摘The purpose of this study is to evaluate the in vivo retention capabilities of poloxamer-based in situ hydrogels for vaginal application with nonoxinol-9 as the model drug. Two in situ hydrogel formulations, which contained 18% poloxamer 407 plus 1% poloxamer 188(GEL1, relative hydrophobic)or 6% poloxamer 188(GEL2, relative hydrophilic), were compared with respect to the rheological properties, in vitro hydrogel erosion and drug release. The vaginal retention capabilities of these hydrogel formulations were further determined in two small animal models, including drug quantitation of vaginal rinsing fluid in mice and isotope tracing with99 m Tc in rats. The two formulations exhibited similar phase transition temperatures ranging from 27 to 32 1C. Increasing the content of poloxamer 188 resulted in higher rheological moduli under body temperature, but slightly accelerated hydrogel erosion and drug release. When compared in vivo, GEL1 was eliminated significantly slower in rat vagina than GEL2,while the vaginal retention of these two hydrogel formulations behaved similarly in mice. In conclusion,increases in the hydrophilic content of formulations led to faster hydrogel erosion, drug release andintravaginal elimination. Rats appear to be a better animal model than mice to evaluate the in situ hydrogel for vaginal application.
