[ Objective] To screen a suitable serum dilution and determine the normal range of antibodies against rabies viruses （RV） in dog serum. [Method]A sensitive, specific and suitable serum dilution was screened. A total...[ Objective] To screen a suitable serum dilution and determine the normal range of antibodies against rabies viruses （RV） in dog serum. [Method]A sensitive, specific and suitable serum dilution was screened. A total of 812 dog serum samples were collected from Changchun, Nanning and Uuzhou regions, and then they were diluted with above screened serum dilution and evaluated by indirect enzyme-linked immunosorbont assay （ELISA）. The positive and negative standard dog sara from the World Organization for Animal Health （OIE） were used as controls. The serum samples with different A450 were randomly selected and the anti-RV serum titers were detected by rapid fluorescent focus inhibition test （RFFIT）. According to the A450 of dog sera, the normal A450 range of negative serum and 95% confidence intervals were calculated with SPSS 10.0 software, and the regression equation of OIE standards was established. [ Result] The screened serum dilution was sensitive and specific; at least 756 negative serum samples were obtained; the normal range and 95% confidence interval of negative serum were 0.127 3 ± 0.059 8 and 0.078 1 -0.172 3, respectively; and the regression equation was A450 = 1.139 serum titer （IU） ±0.470. [ Conclusion] These results lay a foundation for thecontrolling of dog rabies endemic and the development of ELISA kits of dog antibodies against RV.展开更多
<strong>Background:</strong> Aberrant lipid metabolism presumed to have important relationship with gestational diabetes mellitus (GDM), though previous studies revealed inconsistent results on this area.&...<strong>Background:</strong> Aberrant lipid metabolism presumed to have important relationship with gestational diabetes mellitus (GDM), though previous studies revealed inconsistent results on this area.<strong> Objectives:</strong> To identify the difference of serum lipid profile between gestational diabetes mellitus (GDM) and pregnant woman with normal glucose tolerance (NGT). <strong>Methods:</strong> This cross sectional study was conducted from January 2017 to December 2017 at Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh with 31 GDM and equal number of NGT pregnant women diagnosed on the basis of WHO criteria-2013, during 24 - 40 weeks of gestation. Glucose was measured by glucose oxidase method and fasting serum lipid profile [Total cholesterol (TC), High Density Lipoprotein-cholesterol (HDL-C) and Triglyceride (TG)] was measured by enzymatic-colorimetric method. Data were analyzed and compared by statistical tests. <strong>Results: </strong>Among total sixty-two (62) study subjects, 31 were GDM (age: 27.52 ± 4.8 years, body mass index (BMI): 27.17 ± 3.3 kg/m<sup>2</sup>) and 31 were pregnant women with NGT (age: 24.94 ± 4.2 years, BMI: 25.43 ± 6.5 kg/m<sup>2</sup>). Mean age of GDM group was significantly higher than that of NGT group (p = 0.028). Women with GDM showed relatively higher BMI than NGT women but that was not statistically significant (p = 0.194). Fasting lipid profiles between GDM and NGT (GDM vs. NGT;total cholesterol: 194.21 ± 42.18 vs. 208.52 ± 42.18 mg/dl, p = 0.187;HDL-C: 47.50 ± 16.17 vs. 47.18 ± 11.71 mg/dl, p = 0.928;LDL-C: 109.25 ± 28.80 vs. 119.30 ± 34.76 mg/dl, p = 0.220 and triglyceride 204.78 ± 58.50 vs. 202.34 ± 79.18 mg/dl, p = 0.891) were not significantly different. The variations in all lipid fraction values were not statistically significant among GDM women when analyzed between BMI groups holding BMI cut-off at 23 kg/m<sup>2</sup>. No significant differences of any values of lipid profile were found in GDM women according to various age categories (Age < 25 years vs. ≥25 years). <strong>Conclusions: </strong>Lipid profile does not differ between women with GDM and pregnant woman with NGT.展开更多
In this study, normal ranges for hematology and serum biochemistry in children aged 1 to 5 years inUgandawere determined. By a cross-sectional study, 1168 children from Iganga, a prospective site for clinical trials i...In this study, normal ranges for hematology and serum biochemistry in children aged 1 to 5 years inUgandawere determined. By a cross-sectional study, 1168 children from Iganga, a prospective site for clinical trials in Uganda, were screened. From 1168 households, 460 children were selected for enrollment, while 600 (58%) were excluded because of either a history of fever in the previous 24 hours, presence of asexual malaria parasites in the peripheral blood or presence of fever. Accordingly, 460 children (39.4%) of median age = 3 years were enrolled in the baseline study. While the lower limits of hemoglobin, hematocrit levels, mean corpuscular volume and platelet counts for the Ugandan children were found to be less than conventional reference values of Caucasisan children, the white blood cell count reference values were higher than the international intervals. On the other hand, the upper limits of the reference intervals for serum transaminases, bilirubin, creatinine, urea, total protein and albumin in sera of the Ugandan children were higher than the corresponding values for a Caucasian pediatric population. This study showed that, if hematology test results of the Ugandan children were assessed against “imported” international reference values, up to 44.6% of the study participants would have been excluded from clinical trials or would have been reported as adverse events in such trials. The present study was not only the first report of serum biochemistry reference ranges for children aged one to five years in Uganda but also one of very few such studies in Africa.展开更多
BACKGROUND With 4.9 to 35 instances per 100000 cases,hyperparathyroidism is one of the rarest causes of acute pancreatitis.The major cause of primary hyperparathyroidism is a parathyroid adenoma,which can manifest cli...BACKGROUND With 4.9 to 35 instances per 100000 cases,hyperparathyroidism is one of the rarest causes of acute pancreatitis.The major cause of primary hyperparathyroidism is a parathyroid adenoma,which can manifest clinically in various ways.CASE SUMMARY We discuss the unusual case of a 13-year-old boy with recurrent pancreatitis as the initial presentation of primary hyperparathyroidism.The cause of his recurrent pancreatitis remained unknown,and the patient had multiple admissions with acute pancreatitis over 3 years.His diagnosis was delayed due to the initial normal levels of parathyroid hormone,which were later reported elevated in a subsequent episode where ultrasound neck and thyroid scintigraphy revealed a parathyroid adenoma as the underlying cause.After the diagnosis was made,he underwent surgical resection of the adenoma.CONCLUSION This case study stresses the importance of considering uncommon causes for recurrent pancreatitis.展开更多
目的为安全、及时有效地传送艾滋病病毒(HIV)抗体初筛阳性血清,研究在常温下通过邮政特快专递途径传送HIV抗体初筛阳性血清的可行性,以确定温度、震荡、负压环境对HIV抗体阳性血清传送和检测的影响。方法选取不同S/CO值的HIV抗体阳性血...目的为安全、及时有效地传送艾滋病病毒(HIV)抗体初筛阳性血清,研究在常温下通过邮政特快专递途径传送HIV抗体初筛阳性血清的可行性,以确定温度、震荡、负压环境对HIV抗体阳性血清传送和检测的影响。方法选取不同S/CO值的HIV抗体阳性血清做震荡实验和热稳定实验。选用美国AXYGEN公司2.0ml冻存管(SCREW CAP TUBES SCT-200-SS-A)作为HIV抗体阳性血清的传送容器。用稀释墨水做负压实验和破坏实验。结果对于不同反应的HIV抗体阳性样本,在经过50℃加热88小时后,样本免疫印迹(WB)试验反应条带的结果没有任何差异;震荡不会影响HIV抗体检测;负压环境不会发生样本泄漏。常温下通过特快专递方法传送HIV抗体初筛阳性血清,符合《中华人民共和国邮政法》和《中华人民共和国邮政法实施细则》的规定。结论常温下通过邮政特快专递途径传送HIV抗体初筛阳性血清是可行的。展开更多
基金funded by the Ministry of Science and Technology Support Project(2008BAB96B11-3)the Guangxi Science Foundation of China(0728057)+1 种基金the Key Project of Technology Development Plan of Jilin Province(20080930)the Scientific Research Foundation for Introduction of Talent, Jilin University,Faculty of Agriculture(4305050102C1)
文摘[ Objective] To screen a suitable serum dilution and determine the normal range of antibodies against rabies viruses （RV） in dog serum. [Method]A sensitive, specific and suitable serum dilution was screened. A total of 812 dog serum samples were collected from Changchun, Nanning and Uuzhou regions, and then they were diluted with above screened serum dilution and evaluated by indirect enzyme-linked immunosorbont assay （ELISA）. The positive and negative standard dog sara from the World Organization for Animal Health （OIE） were used as controls. The serum samples with different A450 were randomly selected and the anti-RV serum titers were detected by rapid fluorescent focus inhibition test （RFFIT）. According to the A450 of dog sera, the normal A450 range of negative serum and 95% confidence intervals were calculated with SPSS 10.0 software, and the regression equation of OIE standards was established. [ Result] The screened serum dilution was sensitive and specific; at least 756 negative serum samples were obtained; the normal range and 95% confidence interval of negative serum were 0.127 3 ± 0.059 8 and 0.078 1 -0.172 3, respectively; and the regression equation was A450 = 1.139 serum titer （IU） ±0.470. [ Conclusion] These results lay a foundation for thecontrolling of dog rabies endemic and the development of ELISA kits of dog antibodies against RV.
文摘<strong>Background:</strong> Aberrant lipid metabolism presumed to have important relationship with gestational diabetes mellitus (GDM), though previous studies revealed inconsistent results on this area.<strong> Objectives:</strong> To identify the difference of serum lipid profile between gestational diabetes mellitus (GDM) and pregnant woman with normal glucose tolerance (NGT). <strong>Methods:</strong> This cross sectional study was conducted from January 2017 to December 2017 at Department of Endocrinology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh with 31 GDM and equal number of NGT pregnant women diagnosed on the basis of WHO criteria-2013, during 24 - 40 weeks of gestation. Glucose was measured by glucose oxidase method and fasting serum lipid profile [Total cholesterol (TC), High Density Lipoprotein-cholesterol (HDL-C) and Triglyceride (TG)] was measured by enzymatic-colorimetric method. Data were analyzed and compared by statistical tests. <strong>Results: </strong>Among total sixty-two (62) study subjects, 31 were GDM (age: 27.52 ± 4.8 years, body mass index (BMI): 27.17 ± 3.3 kg/m<sup>2</sup>) and 31 were pregnant women with NGT (age: 24.94 ± 4.2 years, BMI: 25.43 ± 6.5 kg/m<sup>2</sup>). Mean age of GDM group was significantly higher than that of NGT group (p = 0.028). Women with GDM showed relatively higher BMI than NGT women but that was not statistically significant (p = 0.194). Fasting lipid profiles between GDM and NGT (GDM vs. NGT;total cholesterol: 194.21 ± 42.18 vs. 208.52 ± 42.18 mg/dl, p = 0.187;HDL-C: 47.50 ± 16.17 vs. 47.18 ± 11.71 mg/dl, p = 0.928;LDL-C: 109.25 ± 28.80 vs. 119.30 ± 34.76 mg/dl, p = 0.220 and triglyceride 204.78 ± 58.50 vs. 202.34 ± 79.18 mg/dl, p = 0.891) were not significantly different. The variations in all lipid fraction values were not statistically significant among GDM women when analyzed between BMI groups holding BMI cut-off at 23 kg/m<sup>2</sup>. No significant differences of any values of lipid profile were found in GDM women according to various age categories (Age < 25 years vs. ≥25 years). <strong>Conclusions: </strong>Lipid profile does not differ between women with GDM and pregnant woman with NGT.
文摘In this study, normal ranges for hematology and serum biochemistry in children aged 1 to 5 years inUgandawere determined. By a cross-sectional study, 1168 children from Iganga, a prospective site for clinical trials in Uganda, were screened. From 1168 households, 460 children were selected for enrollment, while 600 (58%) were excluded because of either a history of fever in the previous 24 hours, presence of asexual malaria parasites in the peripheral blood or presence of fever. Accordingly, 460 children (39.4%) of median age = 3 years were enrolled in the baseline study. While the lower limits of hemoglobin, hematocrit levels, mean corpuscular volume and platelet counts for the Ugandan children were found to be less than conventional reference values of Caucasisan children, the white blood cell count reference values were higher than the international intervals. On the other hand, the upper limits of the reference intervals for serum transaminases, bilirubin, creatinine, urea, total protein and albumin in sera of the Ugandan children were higher than the corresponding values for a Caucasian pediatric population. This study showed that, if hematology test results of the Ugandan children were assessed against “imported” international reference values, up to 44.6% of the study participants would have been excluded from clinical trials or would have been reported as adverse events in such trials. The present study was not only the first report of serum biochemistry reference ranges for children aged one to five years in Uganda but also one of very few such studies in Africa.
文摘BACKGROUND With 4.9 to 35 instances per 100000 cases,hyperparathyroidism is one of the rarest causes of acute pancreatitis.The major cause of primary hyperparathyroidism is a parathyroid adenoma,which can manifest clinically in various ways.CASE SUMMARY We discuss the unusual case of a 13-year-old boy with recurrent pancreatitis as the initial presentation of primary hyperparathyroidism.The cause of his recurrent pancreatitis remained unknown,and the patient had multiple admissions with acute pancreatitis over 3 years.His diagnosis was delayed due to the initial normal levels of parathyroid hormone,which were later reported elevated in a subsequent episode where ultrasound neck and thyroid scintigraphy revealed a parathyroid adenoma as the underlying cause.After the diagnosis was made,he underwent surgical resection of the adenoma.CONCLUSION This case study stresses the importance of considering uncommon causes for recurrent pancreatitis.
文摘目的为安全、及时有效地传送艾滋病病毒(HIV)抗体初筛阳性血清,研究在常温下通过邮政特快专递途径传送HIV抗体初筛阳性血清的可行性,以确定温度、震荡、负压环境对HIV抗体阳性血清传送和检测的影响。方法选取不同S/CO值的HIV抗体阳性血清做震荡实验和热稳定实验。选用美国AXYGEN公司2.0ml冻存管(SCREW CAP TUBES SCT-200-SS-A)作为HIV抗体阳性血清的传送容器。用稀释墨水做负压实验和破坏实验。结果对于不同反应的HIV抗体阳性样本,在经过50℃加热88小时后,样本免疫印迹(WB)试验反应条带的结果没有任何差异;震荡不会影响HIV抗体检测;负压环境不会发生样本泄漏。常温下通过特快专递方法传送HIV抗体初筛阳性血清,符合《中华人民共和国邮政法》和《中华人民共和国邮政法实施细则》的规定。结论常温下通过邮政特快专递途径传送HIV抗体初筛阳性血清是可行的。
