[Objective]To predict prevalence of fleas via a simple epidemiological model.[Method]The field investigation was carried out in 23 locations in Yunnan Province.A simple epidemiological model was involved mean abundanc...[Objective]To predict prevalence of fleas via a simple epidemiological model.[Method]The field investigation was carried out in 23 locations in Yunnan Province.A simple epidemiological model was involved mean abundance and variance of abundance.[Result]The prevalence of fleas increased with their mean abundance in logistic curve.A linear correlation of the predicted prevalence against observed prevalence did not differ significantly from unity.[Conclusion]Prevalence could be explained simply by their mean abundance.展开更多
The Norway lobster,Nephrops norvegicus,is one of the main commercial crustacean fisheries in Europe.The abundance of Nephrops norvegicus stocks is assessed based on identifying and counting the burrows where they live...The Norway lobster,Nephrops norvegicus,is one of the main commercial crustacean fisheries in Europe.The abundance of Nephrops norvegicus stocks is assessed based on identifying and counting the burrows where they live from underwater videos collected by camera systems mounted on sledges.The Spanish Oceanographic Institute(IEO)andMarine Institute Ireland(MIIreland)conducts annual underwater television surveys(UWTV)to estimate the total abundance of Nephrops within the specified area,with a coefficient of variation(CV)or relative standard error of less than 20%.Currently,the identification and counting of the Nephrops burrows are carried out manually by the marine experts.This is quite a time-consuming job.As a solution,we propose an automated system based on deep neural networks that automatically detects and counts the Nephrops burrows in video footage with high precision.The proposed system introduces a deep-learning-based automated way to identify and classify the Nephrops burrows.This research work uses the current state-of-the-art Faster RCNN models Inceptionv2 and MobileNetv2 for object detection and classification.We conduct experiments on two data sets,namely,the Smalls Nephrops survey(FU 22)and Cadiz Nephrops survey(FU 30),collected by Marine Institute Ireland and Spanish Oceanographic Institute,respectively.From the results,we observe that the Inception model achieved a higher precision and recall rate than theMobileNetmodel.The best mean Average Precision(mAP)recorded by the Inception model is 81.61%compared to MobileNet,which achieves the best mAP of 75.12%.展开更多
Hyperactivation is one of the most critical parts for fertilization, cAMP generated by soluble adenylyl cyclase （sAC） is necessary to activate sperm and is a prerequisite for sperm hyperactivation. The aim of this s...Hyperactivation is one of the most critical parts for fertilization, cAMP generated by soluble adenylyl cyclase （sAC） is necessary to activate sperm and is a prerequisite for sperm hyperactivation. The aim of this study is to investigate the function of sAC in hyperactivation in male rats. Four siRNAs of sAC gene were designed and separately transformed into rat sperm using electrotransformation method. Cultured for 12 and 24 h, physiological and biochemical indexes of these sperm were analyzed, and the expressions of some hyperactivation-related genes were detected using real-time PCR. We demonstrated 26.3-30.8% and 49.1-50.5% reduction in sAC at the protein by Western blot and mRNA levels by real-time PCR, respectively. The results showed that two siRNAs, Actb-717 and Actb-4205, were the best RNAi sites for silencing sAC. The VCL （curvilinear velocity） and ALH （amplitude of lateral head displacement） of RNA interference （RNAi）-transfected sperm were reduced, cAMP and protein phosphorylation in RNAi transfected sperm were also decreased. The hyperactivation-related genes, such as CatSper2, LDHC and PKA, were downregulated in the sperm, which sAC was knockdown. These findings demonstrated that sAC might play a critical role in cAMP signaling in the rat sperm hyperactivation, and downregulated sAC gene might prevent the expression of these hyperactivation-ralated genes resulting in sperm dysfunction. These findings suggest that these hyperactivation-ralated genes and sAC are functionally related in sperm hyperactivation and sAC falls into an expanding group of sperm proteins that appear to be promising targets for the development of male contraceptives.展开更多
With aim of place at the disposal of the laboratories nonconventional food with food values and which do not have impact on the system haematopoietic of the rats of laboratory in particular the rat of the species Ratt...With aim of place at the disposal of the laboratories nonconventional food with food values and which do not have impact on the system haematopoietic of the rats of laboratory in particular the rat of the species Rattus norvegicus, the study of the haematologic parameters of rats nourished with nonconventional food makes containing powder of Moringa oleifera was undertaken. Indeed, analysis of the blood taking away carried out following the administration of five diets container various rates of powder of Moringa oleifera (0% (Control food), 25%, 50%, 75% and 100%) respectively codified L3P, L3P25, L3P50, L3P75 and L3P100. These different food formulations were administered to rats (Ratus norvegicus) during three months. The weight of these animals was daily taken, followed by blood samples every 30 days for the determination of the blood count. The results of the study were shown differences between the various food formulations for each period of blood samples in biological parameters. These differences were more significant for platelets. During the three months of study, the hematocrit, platelets, MCV and outside the control group, were reported a slight progressive increase. The leukocyte parameters of all the rats in the investigation, indicated an increase in the second month. In conclusion, the study shows that during the three months of investigation, the tested foods do not disturb the haematological parameters except for thrombocytes which reveal a modification.展开更多
基金Supported by Yunnan Provincial Foundation for Key Scientific Projects (2007C0005Z2)~~
文摘[Objective]To predict prevalence of fleas via a simple epidemiological model.[Method]The field investigation was carried out in 23 locations in Yunnan Province.A simple epidemiological model was involved mean abundance and variance of abundance.[Result]The prevalence of fleas increased with their mean abundance in logistic curve.A linear correlation of the predicted prevalence against observed prevalence did not differ significantly from unity.[Conclusion]Prevalence could be explained simply by their mean abundance.
基金Open Access Article Processing Charges has been funded by University of Malaga.
文摘The Norway lobster,Nephrops norvegicus,is one of the main commercial crustacean fisheries in Europe.The abundance of Nephrops norvegicus stocks is assessed based on identifying and counting the burrows where they live from underwater videos collected by camera systems mounted on sledges.The Spanish Oceanographic Institute(IEO)andMarine Institute Ireland(MIIreland)conducts annual underwater television surveys(UWTV)to estimate the total abundance of Nephrops within the specified area,with a coefficient of variation(CV)or relative standard error of less than 20%.Currently,the identification and counting of the Nephrops burrows are carried out manually by the marine experts.This is quite a time-consuming job.As a solution,we propose an automated system based on deep neural networks that automatically detects and counts the Nephrops burrows in video footage with high precision.The proposed system introduces a deep-learning-based automated way to identify and classify the Nephrops burrows.This research work uses the current state-of-the-art Faster RCNN models Inceptionv2 and MobileNetv2 for object detection and classification.We conduct experiments on two data sets,namely,the Smalls Nephrops survey(FU 22)and Cadiz Nephrops survey(FU 30),collected by Marine Institute Ireland and Spanish Oceanographic Institute,respectively.From the results,we observe that the Inception model achieved a higher precision and recall rate than theMobileNetmodel.The best mean Average Precision(mAP)recorded by the Inception model is 81.61%compared to MobileNet,which achieves the best mAP of 75.12%.
基金supported by the National Natural Science Foundation of China (30471243)the National Key Technology R&D Program of China (2011BAD28B02,2012BAD12B10)the Provincial Project for Agricultural Techniques of China (SX2011-189)
文摘Hyperactivation is one of the most critical parts for fertilization, cAMP generated by soluble adenylyl cyclase （sAC） is necessary to activate sperm and is a prerequisite for sperm hyperactivation. The aim of this study is to investigate the function of sAC in hyperactivation in male rats. Four siRNAs of sAC gene were designed and separately transformed into rat sperm using electrotransformation method. Cultured for 12 and 24 h, physiological and biochemical indexes of these sperm were analyzed, and the expressions of some hyperactivation-related genes were detected using real-time PCR. We demonstrated 26.3-30.8% and 49.1-50.5% reduction in sAC at the protein by Western blot and mRNA levels by real-time PCR, respectively. The results showed that two siRNAs, Actb-717 and Actb-4205, were the best RNAi sites for silencing sAC. The VCL （curvilinear velocity） and ALH （amplitude of lateral head displacement） of RNA interference （RNAi）-transfected sperm were reduced, cAMP and protein phosphorylation in RNAi transfected sperm were also decreased. The hyperactivation-related genes, such as CatSper2, LDHC and PKA, were downregulated in the sperm, which sAC was knockdown. These findings demonstrated that sAC might play a critical role in cAMP signaling in the rat sperm hyperactivation, and downregulated sAC gene might prevent the expression of these hyperactivation-ralated genes resulting in sperm dysfunction. These findings suggest that these hyperactivation-ralated genes and sAC are functionally related in sperm hyperactivation and sAC falls into an expanding group of sperm proteins that appear to be promising targets for the development of male contraceptives.
文摘With aim of place at the disposal of the laboratories nonconventional food with food values and which do not have impact on the system haematopoietic of the rats of laboratory in particular the rat of the species Rattus norvegicus, the study of the haematologic parameters of rats nourished with nonconventional food makes containing powder of Moringa oleifera was undertaken. Indeed, analysis of the blood taking away carried out following the administration of five diets container various rates of powder of Moringa oleifera (0% (Control food), 25%, 50%, 75% and 100%) respectively codified L3P, L3P25, L3P50, L3P75 and L3P100. These different food formulations were administered to rats (Ratus norvegicus) during three months. The weight of these animals was daily taken, followed by blood samples every 30 days for the determination of the blood count. The results of the study were shown differences between the various food formulations for each period of blood samples in biological parameters. These differences were more significant for platelets. During the three months of study, the hematocrit, platelets, MCV and outside the control group, were reported a slight progressive increase. The leukocyte parameters of all the rats in the investigation, indicated an increase in the second month. In conclusion, the study shows that during the three months of investigation, the tested foods do not disturb the haematological parameters except for thrombocytes which reveal a modification.
