The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting...The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments,saving people's lives and preventing epidemics.It is important to establish a quick standard diagnostic test for the detection of the infectious disease(COVID-19)to prevent subsequent secondary spread.Polymerase chain reaction(PCR)is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity.Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler opera-tion.A variety of improved or new approaches also have been developed.This review summarizes the currently available detection methods for coronavirus nucleic acid.It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coro-navirus infection.展开更多
Since the outbreak of coronavirus disease 2019(COVID-19),the author has been involved in the treatment of COVID-19 in the first line.It is found that some patients had a long viral nucleic acid negative time,and even ...Since the outbreak of coronavirus disease 2019(COVID-19),the author has been involved in the treatment of COVID-19 in the first line.It is found that some patients had a long viral nucleic acid negative time,and even the course of the disease lasted more than 40 days.After a thorough investigation of the root causes,it is found the four possible causes were:"Blood stasis syndrome"is difficult to remove;"Damp heat"sticky greasy;Stubborn basic diseases;weak constitution.All the patients treated with traditional Chinese medicine have turned negative,and now the typical cases were analyzed and summarized in order to provide reference for the traditional Chinese medicine treatment and scientific research of the patients with COVID-19 who were difficult to turn negative.展开更多
The novel coronavirus(2019 novel coronavirus,2019-nCoV) is a newly emerged virus.Therefore,the nucleic acid detection technology and serum immunological detection technology established urgently in emergencies have ce...The novel coronavirus(2019 novel coronavirus,2019-nCoV) is a newly emerged virus.Therefore,the nucleic acid detection technology and serum immunological detection technology established urgently in emergencies have certain defects inevitably.The efficiency of nucleic acid and antibody detection varies at different stages of disease progression of the novel coronavirus disease(COVID-19).If the two can be used in combination and the results of the analysis can be comprehensively judged,they can be combined to monitor the viral load and disease progress better and improve the diagnostic efficiency.Based on the author’s application experience,this article analyzes and interprets eight common modes of combined detection of nucleic acids and specific antibodies in order to provide a reference for the clinical diagnosis and treatment of COVID-19.展开更多
基金financial support from the National Natural Science Foundation of China(Grant 81973281)the Fundamental Research Funds for the Central Universities(2019FZA7017)Leading Talent of“Ten Thousand Plan”-National High-Level Talents SpecialSupport Plan。
文摘The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments,saving people's lives and preventing epidemics.It is important to establish a quick standard diagnostic test for the detection of the infectious disease(COVID-19)to prevent subsequent secondary spread.Polymerase chain reaction(PCR)is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity.Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler opera-tion.A variety of improved or new approaches also have been developed.This review summarizes the currently available detection methods for coronavirus nucleic acid.It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coro-navirus infection.
基金Science and Technology Research Project of Jiangxi Provincial Department of Science and Technology(No.S2020ZPYFG0006)Training Project of National Traditional Chinese Medicine Innovative Key Talents National Chinese Medicine(2019)(No.128)
文摘Since the outbreak of coronavirus disease 2019(COVID-19),the author has been involved in the treatment of COVID-19 in the first line.It is found that some patients had a long viral nucleic acid negative time,and even the course of the disease lasted more than 40 days.After a thorough investigation of the root causes,it is found the four possible causes were:"Blood stasis syndrome"is difficult to remove;"Damp heat"sticky greasy;Stubborn basic diseases;weak constitution.All the patients treated with traditional Chinese medicine have turned negative,and now the typical cases were analyzed and summarized in order to provide reference for the traditional Chinese medicine treatment and scientific research of the patients with COVID-19 who were difficult to turn negative.
文摘The novel coronavirus(2019 novel coronavirus,2019-nCoV) is a newly emerged virus.Therefore,the nucleic acid detection technology and serum immunological detection technology established urgently in emergencies have certain defects inevitably.The efficiency of nucleic acid and antibody detection varies at different stages of disease progression of the novel coronavirus disease(COVID-19).If the two can be used in combination and the results of the analysis can be comprehensively judged,they can be combined to monitor the viral load and disease progress better and improve the diagnostic efficiency.Based on the author’s application experience,this article analyzes and interprets eight common modes of combined detection of nucleic acids and specific antibodies in order to provide a reference for the clinical diagnosis and treatment of COVID-19.
