Molecular phylogeny and evolution of Scomber(Teleostei:Scombridae)based on mitochondrial and nuclear DNA sequences

A molecular phylogenetic analysis of the genus Scomber was conducted based on mitochondrial(COI,Cyt b and control region) and nuclear(5S rDNA) DNA sequence data in multigene perspective.A variety of phylogenetic analytic methods were used to clarify the current taxonomic classification and to assess phylogenetic relationships and the evolutionary history of this genus.The present study produced a well-resolved phylogeny that strongly supported the monophyly of Scomber.We confirmed that S.japonicus and S.colias were genetically distinct.Although morphologically and ecologically similar to S.colias,the molecular data showed that S.japonicus has a greater molecular affinity with S.australasicus,which conflicts with the traditional taxonomy.This phylogenetic pattern was corroborated by the mtDNA data,but incompletely by the nuclear DNA data.Phylogenetic concordance between the mitochondrial and nuclear DNA regions for the basal nodes supports an Atlantic origin for Scomber.The present-day geographic ranges of the species were compared with the resultant molecular phylogeny derived from partition Bayesian analyses of the combined data sets to evaluate possible dispersal routes of the genus.The present-day geographic distribution of Scomber species might be best ascribed to multiple dispersal events.In addition,our results suggest that phylogenies derived from multiple genes and long sequences exhibited improved phylogenetic resolution,from which we conclude that the phylogenetic reconstruction is a reliable representation of the evolutionary history of Scomber.

Phosphatidylglycerol-containing ER-transport vesicles built and restore outer mitochondrial membrane and deliver nuclear DNA translation products to generate cardiolipin in the inner mitochondrial membrane

Phosphatidylglycerol (PG) an important membrane phospholipid required for the synthesis of diphos-phatidylglycerol (DPG) commonly known as cardiolipin (CL) was identified in the fraction of endo-plasmic reticulum (ER)-derived transport vesicles which had no affinity for Golgi. The vesicles were produced in the presence of Brefeldin A (BFA), the agent known to inhibit ER-Golgi transport, and found to display affinity to mitochondria. The analysis revealed that their cargo was not containing proteins that are transported to Golgi, and that their membrane was free of phosphatidylinositol (PI) and ceramides (Cer). The incubation of PG-containing transport vesicles with mitochondria afforded incorporation of their membrane into the Outer Mito-chondrial Membrane (OMM) and formation of lyso-phosphatidylglycerol (LPG). In turn, upon further incubation with fresh transport active cytosol, the mitochondrial LPG was converted to PG. The results of analysis of the OMM, Inner Mitochondrial Mem-brane (IMM) and Inner Mitochondrial Space Components (IMSC) strongly suggest that PG-containing transport vesicles deliver nuclear DNA translation products to the IMSC and thus facilitate CL synthesis in the IMM. In summary, our studies provide evidence that ER-generated PG-enriched transport vesicles represent the general pathway for restitution of mitochondrial membranes and the delivery of nuclear DNA translation products that generate CL, and thus sustain the mitochondrial matrix CL-dependent metabolic reactions.

Differences in Nuclear DNA Between Male-Sterile and Male-Fertile Lines of Sorghum bicolor

Cytoplasmic male sterility (cms) is determined by nuclear-cytoplasmic interactions. Up tonow, most studies are focused on the comparison of cytoplasmic DNAs of male-sterile lines and male-fertilelines, and analysis of nuclear DNA has not been documented yet. In order to find out the possible difference innuclear genome of male-sterile line A1 Tx623 and corresponding male-fertile line Tx623 of sorghum, randomamplified polymorphic DNA (RAPD) approach was used to analyze their cytoplasmic and nuclear genomes.Total DNAs of them were amplified at first to screen primers, which were able to generate reproducible bandsspecific to male-sterile line or male-fertile line. Then the selected primers were used to amplify their mitochon-drial DNA (mtDNA) and chloroplast DNA (cpDNA). The origins of all the polymorphic fragments were ana-lyzed. After ruling out those amplified from cytoplasmic DNA, seventeen polymorphic fragments were deter-mined to be amplified from nuclear DNA. These fragments originated from nuclear DNA indicate that diffe-rences in sequence exist between the nuclear DNA of male-sterile line and male-fertile line of sorghum, whichdo not agree with the traditional standpoint that they have identical nucleus.

Conserved balance of hepatocyte nuclear DNA content in mononuclear and binuclear hepatocyte populations during the course of chronic viral hepatitis

瞄准：到有增加的原子 DNA 的 hepatocytes 的百分比满足的分析，即，四倍的(4n ) 和 octoploid (8n ) 原子核，然后比较单音原子并且双性人原子 hepatocyte 人口。方法：百分比单音原子双( 2n )，双性人的 4n ，和 8n hepatocytes 和那些原子 2 x 2n ， 2 x 4n ，并且 2 x 8n hepatocytes 与一个方法被决定能同时与长期的肝炎 B 或 C 在 62 个病人测量原子 DNA 满足的 hepatocyte 和 binuclearity 。在单音的原子 hepatocyte 人口的 4n 和 8n hepatocytes 的百分比在双性人与 2 x 4n 和 2 x 8n hepatocytes 的百分比相比原子 hepatocyte 人口。结果：在双性人的在单音的原子 hepatocytes 的 4n 和 2 x 4n 和 2 x 8n hepatocytes 的百分比原子 hepatocytes 是类似的，不管这项活动或长期的肝炎并且不管感染的病毒的纤维变性等级。结论：在内的原子 DNA 内容的分发单音原子并且双性人原子 hepatocyte 人口在长期的病毒的肝炎的功课期间被保存。

精子核DNA完整性测定方案的优化及其在辅助生殖技术中的应用价值

目的:对现有精子核DNA完整性测定方案进行优化,并探讨其在辅助生殖技术(ART)中的应用价值。方法:选择2021年1月1日至2022年12月8日于江西中医药大学附属生殖医院就诊的拟接受体外受精-胚胎移植(IVF-ET)助孕的194对夫妇作为研究对象。采集男方的精液作为对照组(n=194),同一精液经双层密度梯度离心法优化处理后精子混合液作为观察组(n=194)。根据精子核DNA碎片率(DFI)测定结果将对照组及观察组各分为3个亚组,对照A组和观察A组:DFI<15%,对照B组和观察B组:DFI 15%~30%,对照C组和观察C组:DFI≥30%。对观察组与对照组的DFI值及各亚组间的助孕及妊娠情况进行比较。结果:(1)观察组DFI明显低于对照组,差异有统计学意义[(13.55±10.17)%vs.(18.56±11.54)%,P<0.05]。(2)6个亚组间的受精率、卵裂率及优胚率两两比较,差异无统计学意义(P>0.05)。(3)6个亚组的妊娠率和着床率比较,差异有统计学意义(P<0.05);其中,对照A组、对照B组、观察A组、观察B组4组的临床妊娠率(均在65.00%以上)及着床率(均在50.00%)两两比较,差异均无统计学意义(P>0.05),但均高于对照C组(43.24%、31.67%)及观察C组(13.64%、8.82%),差异有统计学意义(P<0.05);对照C组的临床妊娠率及着床率明显高于观察C组,差异有统计学意义(P<0.05)。结论:精液经优化处理后精子核DNA完整性可明显改善。两种不同检测方案在ART中均有较好的应用价值,当精液经优化处理后DFI≥30%时,对ART的不良妊娠结局具有更好的预测价值。

Analysis of plastid and nuclear DNA data in plant phylogenetics——evaluation and improvement

Correct combination of plastid(cp)and nuclear(nr)DNA data for plant phylogenetic reconstructions is not a new issue,but with an increasing number of nrDNA loci being used,it is of ever greater practical concern.For accurately reconstructing the phylogeny and evolutionary history of plant groups,correct treatment of phylogenetic incongruence is a vital step in the proper analysis of cpDNA and nrDNA data.We first evaluated the current status of analyzing cpDNA and nrDNA data by searching all articles published in the journal Systematic Botany between 2005 and 2011.Many studies combining cpDNA and nrDNA data did not rigorously assess the combinability of the data sets,or did not address in detail possible reasons for incongruence between the two data sets.By reviewing various methods,we outline a procedure to more accurately analyze and/or combine cpDNA and nrDNA data,which includes four steps:identifying significant incongruence,determining conflicting taxa,providing possible interpretations for incongruence,and reconstructing the phylogeny after treating incongruence.Particular attention is given to explanation of the cause of incongruence.We hope that our procedure will help raise awareness of the importance of rigorous analysis and help identify the cause of incongruence before combining cpDNA and nrDNA data.

Phylogeny of Ptychostomum (Bryaceae,Musci) inferred from sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) and chloroplast rps4

摘要 Ptychostomum 的发展史首先基于内部抄录分隔符的分析被承担(它的) 原子 ribosomal (nr ) 的区域 DNA 并且由从 nrDNA 联合数据，它的 and 叶绿体 DNA rps4 定序。最大的吝啬，最大的可能性，和贝叶斯的分析都支持使复原的类 Ptychostomum 不是单音的结论门。Ptychostomum funkii (Schw&#228;gr.)J. R。Spence (&#8801;Bryum funkii Schw&#228;gr.) 被放在包含 Bryum 的类型种类的 clade 以内， B。argenteum Hedw。在现在的学习调查的 Ptychostomum 的留下的成员组成另一支持得好的 clade。结果与以前的分子的分析适合。根据种系发生的证据，我们同意转的 B。amblyodon M ü l l。哈尔。（ &#8801;B。inclinatum (Brid ) Turton &#8801;Bryum 天使长嗯 Bruch 与 Schimp ) ， Bryum lonchocaulon M ü l l。哈尔， Bryum pallescens Schleich。前 Schw&#228;gr. ，和 Bryum 伙伴透镜 Sw。到 Ptychostomum。

人卵泡液游离DNA提取及定量检测方法的比较

目的:比较不同的DNA提取方法、实时荧光定量PCR(qPCR)的引物及荧光染料对人卵泡液游离核DNA(cf-nDNA)和游离线粒体DNA(cf-mtDNA)定量检测的影响。方法:收集2018年3月~10月在华中科技大学同济医学院生殖医学中心进行体外受精/卵胞浆内单精子显微注射技术(IVF/ICSI)的40例患者卵泡液样本,分别用4种不同的方法(方法一:BeaverBeads^(TM) Circulating DNA试剂盒、方法二:蛋白酶K(PK)法、方法三:Hieff^(■) qPCR SYBR^(■) Green Master Mix和方法四:KOD SYBR^(■) qPCR Mix)提取和纯化卵泡液中的游离DNA(cf-DNA)样本;用4种cf-nDNA引物(ALU115、B2MF1、GAPDH和β-globin)和4种cf-mtDNA引物(ND1-primer1、ND1-primer2、hmito3和hmito5)分别定量检测卵泡液cf-nDNA和cf-mtDNA水平;比较qPCR实验中SYBR Green化学染料和TaqMan探针两种方法在定量检测上的差异。结果:提取cf-nDNA的浓度按以下顺序排列:方法一>方法四>方法二>方法三(P<0.05),提取cf-mtDNA的浓度按以下顺序排列:方法一>方法四>方法三>方法二(P<0.05);样本中cf-nDNA的扩增浓度按照以下顺序排列:ALU115>GAPDH>β-globin>B2MF1(P<0.05),4种cf-nDNA引物无相关性(P>0.05)。cf-mtDNA的扩增浓度按以下顺序排列:ND1-primer1>ND1-primer2>hmito5>hmito3(P<0.05)。ND1-primer1和ND1-primer2有很强的相关性(r=0.517,P<0.05),hmito3和hmito5也有很强的相关性(r=0.989,P<0.05);在以β-globin和ND1-primer1为引物的qPCR实验中,TaqMan探针法检测的Ct值高于SYBR Green化学染料法(P<0.05)。结论:人卵泡液样本的提取方法、引物和荧光染料的选择均影响cf-nDNA和cf-mtDNA的定量,建议根据不同的研究目的选择合适的方法。

EFFECTS OF PERIOPERATIVE CIMETIDINE ADMINISTRATION ON TUMOR CELL NUCLEAR MORPHOMETRY AND DNA CONTENT IN PATIENTS WITH GASTROINTESTINAL CANCER

Objective: To explore the effects of perioperative cimetidine administration on tumor cell nuclear morphometric parameters and DNA content in patients with gastrointestinal adenocarcinoma. Methods: 49 patients with pathologically confirmed gastrointestinal adenocarcinoma were randomized into test group (n=25) and control group (n=24). The test group started oral cimetidine intake 400 mg, tid, 7-10d before operation, followed by standard curative operation. The control group did not receive cimetidine. armor specimens were paraffin embedded for microsection and stained with hematoxylin and eosin (HE) and Feulgen stain. Morphometric studies and DNA colltent of tumor nuclei were performed on IBAS Image Analyzer. Results: The tumor cell nuclear area (μm2), nuclear perimeter (μm), maximal nuclear diameter (μm) for test group/control group were 23.54 ±5.08134.69±10.08 (P<0.001), 22.06±4.43/24.88±4.05 (P<0.05), 7.84±1.64/ 8.62±1.24 (P<0.05), 4.42±0.6115.41±0.89 (P<0.001), Respectively. The percentages (%) of diploidy, triple-tetraploidy, quintuple ploidy, and >quintuple ploidy tumor cells for test group/control group were 16.64±2.58/5.33±2.14 (P<0.002), 39.84±2.28/35.70±3.58 (P>0.50), 12.42±5.00/14.48±0.74 (P>0.20), 31.11±6.86/ 45.97±3.82 (P<0.005), respectively. Conclusion: Periopcrative administration of cimetidine in gasgtrointestinal cancer patients could decrease the nuclear size and raise the percentage of diploid tumor cells, and convert high aneuploid tumor cells into low-aneuploid tumor cells, which might help reduce the invasiveness of tumor cells.

Genetic variation and phylogenetic relationship of Hypoderaeum conoideum(Bloch, 1782) Dietz, 1909(Trematoda: Echinostomatidae) inferred from nuclear and mitochondrial DNA sequences

Objective:To explore genetic variations of Hypoderaeum conoideum collected from domestic ducks from 12 different localities in Thailand and Lao PDR,as well as their phylogenetic relationship with American and European isolates.Methods:The nucleotide sequences of their nuclear ribosomal DNA(ITS),mitochondrial cytochrome c oxidase subunit 1(CO1),and NADH dehydrogenase subunit 1(ND1)were used to analyze genetic diversity indices.Results:We found relatively high levels of nucleotide polymorphism in ND1(4.02%),whereas moderate and low levels were observed in CO1(2.11%)and ITS(0.96%),respectively.Based on these polymorphisms,the 20 ND1,12 CO1,and 18 ITS haplotypes were classified,and several common haplotypes were observed in all samples.At least three major lineages,namely American,European and Asian lineages,have been classified by phylogenetic analyses based on ND1 sequences.Conclusions:Our report demonstrates that the ND1 gene is the most suitable genetic marker to explore genetic variation and phylogenetic relationship of Hypoderaeum conoideum.However,a combination of all loci for ND1,CO1 and ITS would be of great value toward further genetic investigation of this endemic worldwide parasite.Thus,comprehensive molecular genetic analyses of Hypoderaeum conoideum from its worldwide distribution is needed to further understanding of the evolutionary and systematic relationships of this parasite.

Interspecific Hybridization between <i>Arisaema sikokianum</i>and <i>A. serratum</i>(Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons

A morphologically intermediate plant between Arisaema sikokianum Franch. et Sav. and A. serratum (Thunb.) Schott has been newly found in Kochi Prefecture, Shikoku, Japan. The putative hybrid has the intermediate morphological characteristics of the parental species. Molecular analysis using PCR-RFLP of internal transcribed spacer (ITS) in nuclear DNA (nrDNA) indicates that the putative hybrid has a combined pattern of the two putative parent species. Moreover, the sequence result of chloroplast DNA (cpDNA) of the putative hybrid was identical to that of A. sikokianum. These results suggest that the putative hybrid is a hybrid between A. sikokianum and A. serratum and that it was formed by interactive gene exchanging via pollens from A. serratum to A. sikokianum. It is the first record of a hybrid between A. sikokianum and A. serratum.

基于1H-NMR和16S rDNA测序技术探讨针与灸不同刺激方法对大鼠结肠代谢物和肠道菌群的影响

目的:观察针刺与艾灸两种不同干预方法对健康SD大鼠结肠代谢物和肠道菌群调节作用的差异。方法:将33只健康SD大鼠随机分为空白组、针刺组和艾灸组,每组各11只。空白组大鼠仰卧固定在治疗台上,不予任何干预,连续7 d;针刺组以不锈钢针灸针针刺大鼠双侧上巨虚穴和天枢穴,留针15 min,连续干预7 d;艾灸组采用温和灸,将艾条置于大鼠双侧上巨虚穴和天枢穴上方3~5 cm处,使皮肤温度维持在(45±5)℃之间,每次15 min,连续干预7 d。干预结束后,取各组大鼠结肠组织和粪便样品,运用质子核磁共振(proton nuclear magnetic resonance,1H-NMR)技术检测各组大鼠结肠组织代谢物,分析并筛选大鼠结肠组织差异代谢物;16S核糖体DNA(ri-bosomal DNA,rDNA)测序技术检测各组大鼠肠道菌群丰度和多样性的变化情况,并比较差异菌群。结果:代谢组学检测结果显示,与空白组相比,针刺组大鼠结肠组织中代谢物组氨酸、缬氨酸和丁酸含量显著上升(P<0.05),艾灸组大鼠结肠组织中代谢物胆碱和肌醇含量显著上升(P<0.05或P<0.01);与针刺组相比,艾灸组大鼠结肠组织中代谢物甲酸和乙酸含量显著上升(P<0.05)。菌群多样性分析显示,与空白组相比,针刺组和艾灸组大鼠肠道菌群的Chao1指数和基于丰度的覆盖估计值(abundance-based coverage estimator,ACE)均显著上升(P<0.05);在菌群门和属水平上,针、灸干预后部分有益菌丰度上升,部分条件致病菌丰度下降。结论:针刺与艾灸两种不同干预方法对健康SD大鼠结肠代谢物和肠道菌群的调节存在差异性:针刺更擅长调节酸类代谢失衡所导致的胃肠道疾病,艾灸更适用于脂质代谢异常引发的代谢类疾病;针刺与艾灸对肠内微生态环境具有良性调节作用;针刺能通过影响肠道菌群的结构和丰度缓解胃肠道疾病。

男性不育症患者精子核DNA完整性的临床研究

目的:探讨男性不育症患者的精子核DNA完整性情况及治疗措施。方法:选取2022年5—8月在我院生殖医学中心诊治的210例男性不育症患者作为研究对象。收集分析精液样本,根据常规参数的参考值将患者分为正常精液组、少精子症组、弱精子症组和畸形精子症组,再对四组患者的精子核DNA完整性进行检测,比较精子DNA碎片指数(DFI)。按照DFI值将患者分为精子核DNA完整性正常组和异常组,比较常规参数精子浓度、活动力、前向运动精子率(PR)和正常形态精子率。分析患者精子DFI与常规参数之间的相关性。给予精子核DNA完整性异常组患者左卡尼汀(LC)联合辅酶Q_(10)(CoQ_(10))治疗,比较治疗前后的DFI和常规参数。结果:少精子症组、弱精子症组、畸形精子症组和正常精液组的DFI比较,差异有统计学意义(P<0.05)。与精子核DNA完整性正常组比较,DNA完整性异常组的精子浓度、活动力、PR和正常形态精子率均偏低,差异有统计学意义(P<0.05)。相关性分析得出男性不育症患者的精子DFI与所测常规参数之间呈负相关。经LC联合CoQ_(10)治疗后,DNA完整性异常组患者的精子浓度、活动力、PR和正常形态精子率较治疗前增高,而DFI值较治疗前有所降低,差异有统计学意义(P<0.05)。结论:精子核DNA完整性主要与精液质量相关,临床实践中采用LC联合CoQ_(10)对精子核DNA完整性异常的患者予以治疗,可有效改善其精液质量并减轻DNA损伤。

Regulation of eukaryotic DNA replication and nuclear structure

In eukaryote, nuclear structure is a key component forthe functions of eukaryotic cells. More and more evidencesshow that the nuclear structure plays important role in re-gulating DNA replication. The nuclear structure providesa physical barrier for the replication licensing, participatesin the decision where DNA replication initiates, and orga-nizes replication proteins as replication factory for DNAreplication. Through these works, new concepts on theregulation of DNA replication have emerged, which willbe discussed in this minireview.

植物增殖细胞核抗原与DNA复制关系的研究现状

为了更深入的对植物增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)进行研究,本研究归纳了PCNA的研究现状,总结了PCNA的结构特征和包括PCNA参与DNA复制、DNA损伤修复和细胞周期调控等在内的PCNA的功能特点。目前有关PCNA的研究以医学和动物体为主,有关植物PCNA研究的报道很少且相对落后,但已有文献指出,PCNA调控DNA复制,参与DNA复制以确保植物体正常生长发育,因此植物PCNA的功能值得继续研究。

Using Nuclear Transport Theory to Study Nonlinear Effects Between the Different Strands in DNA

A dynamic model of DNA molecules which takes into account the interaction of the nearestbase pairs between the different strands is presented.The nonlinear effect and phase transition aresimulated by the nuclear transport theory and the method of fractorial moment in high energy physics,re-spectively.The results show that the nonlinear interaction of the nearest base pairs between the differentstrands may play a rule in DNA molecules.

基于3个基因16种胡椒鲷分子系统进化关系

为从分子水平分析胡椒鲷属鱼类分子系统分类关系,澄清物种分类争议,采集印度-西太平洋分布的胡椒鲷鱼类16种,利用PCR扩增及测序方法获得这16种鱼类线粒体Cyt b及核RAG2、S7标记序列,基于最大似然法与贝叶斯法构建分子系统进化关系。结果显示:16种鱼类Cyt b同源序列为657 bp,编码219个氨基酸;RAG2同源序列为726 bp,编码243个氨基酸;S7基因同源序列为729 bp,序列存在大量碱基插入与缺失。以大斑石鲈及断斑石鲈为外类群构建分子系统进化树,进化树上胡椒鲷鱼类主要分为3个分支,分支Ⅰ胡椒鲷具鲜艳的体色与斑纹,分支Ⅱ与分支Ⅲ胡椒鲷色彩单一,无鲜艳斑纹,与形态分类结果相似。少棘胡椒鲷属在进化树上并未形成独立的分支,而是位于胡椒鲷属内部。比较分析少棘胡椒鲷属、胡椒鲷属鱼类鳍棘数目与各物种在进化树上的分类关系,并未发现鳍棘数目与物种亲缘关系存在相关性。研究结果表明,在胡椒鲷类群中,背鳍鳍棘数可作为形态上区分不同胡椒鲷种类的指标,但未必能作为胡椒鲷鱼类亲缘关系远近的划分依据。结果支持目前大多数分子分类研究结果,少棘胡椒鲷鱼类应归为胡椒鲷属。

血清中抗核抗体与抗双链DNA抗体联合检测对系统性红斑狼疮的诊断价值

目的:研究联合检测血清内抗核抗体(ANA)及抗双链DNA抗体对系统性红斑狼疮(SLE)的诊断价值。方法:选取2022年1月—2023年1月贵州省兴义市人民医院收治的SLE患者68例为试验组,同期在院进行检测的健康者68例为对照组。检测两组血清ANA以及血清抗双链DNA抗体,比较两组检测结果阳性率。结果:试验组的血清抗双链DNA抗体检测、血清ANA检测、血清抗双链DNA抗体+ANA检测阳性率均高于对照组,差异有统计学意义(P<0.001)。结论:联合检测血清中ANA、抗双链DNA抗体,对于诊断SLE有极高的应用价值。

ANA、抗ENA抗体联合抗ds-DNA抗体对自身免疫性疾病的诊断价值

目的 研究自身免疫性疾病诊断中抗核抗体(ANA)、抗可提取性核抗原抗体(抗ENA抗体)、抗双链DNA抗体(抗ds-DNA抗体)联合诊断的临床价值。方法 选取240例自身免疫性疾病患者为观察组,另选取240例健康体检者为对照组。两组研究对象均测定血清抗ENA抗体、ANA及抗ds-DNA抗体。对比两组血清ANA、抗ds-DNA抗体、抗ENA抗体阳性率,观察组不同疾病患者的抗ENA抗体阳性率。结果 观察组血清抗ds-DNA抗体阳性率60.42%、ANA阳性率82.08%、抗ENA抗体阳性率64.58%均显著高于对照组的0.42%、0.42%、0,差异具有统计学意义(P<0.05)。观察组不同疾病患者的抗nRNP/Sm抗体、抗Sm抗体、抗SS-A抗体、抗Ro-52抗体、抗SS-B抗体、抗SCL-70抗体、抗PM-SCL抗体、抗J0-1抗体阳性率对比,差异具有统计学意义(P<0.05)。结论 自身免疫性疾病中ANA、抗ENA抗体、抗ds-DNA抗体联合检测,可为临床诊治提供可靠依据。

流式细胞仪检测高等植物细胞核DNA含量的方法

相对于动物和微生物而言,流式细胞术在植物科学上的应用会因植物组织与细胞(如细胞壁、中央液泡、特殊细胞器等)的特殊结构以及次生代谢产物等特殊成分,造成样品在前期处理、染色及测试等方面的困难,甚至导致检测失败或结果不准确。笔者在长期运用流式细胞仪测试工作中,积累了大量的植物样本检测经验,并参考国内外相关文献,总结出从植物取材、样品制备到植物细胞核DNA流式检测的方法和技巧,可为植物科学研究者及从事流式细胞检测的技术人员提供实验参考。