The cotton bollworm Helicoverpa armigera and the oriental tobacco budworm H. assulta are sibling species, both being important agricultural pests. Morphologically, the two insects are almost indistinguishable at the e...The cotton bollworm Helicoverpa armigera and the oriental tobacco budworm H. assulta are sibling species, both being important agricultural pests. Morphologically, the two insects are almost indistinguishable at the egg, larval and pupal stages. One of the big challenges in the study of these insects, in particular in integrated pest management, is a timely and dependable identification of these insects at their early stages of development. Here, we report a H. armigera-specific nuclear DNA marker, and demonstrate that it can be employed to reliably discriminate between 1-1. armigera and 11. assulta by simple polymerase chain reaction amplification experiment.展开更多
文摘综述了有关线粒体基因组 (m t DNA)多态性与运动能力的研究 :mt DNA为 16 5 6 9bp的双链闭环分子 ,含有 37个基因 ,排列紧密 ,无内含子。其转录、复制受核 DNA调控 ,同时也不同程度地影响核 DNA表达 ;mt DNA的多样性很高的结论被进一步证实 ,且 m t DNA的差异主要分布在群体内的个体间 ,而群体间的差异较小 ;目前研究认为 ,m t DNA多态性可能造成群体中有氧代谢能力的个体差异 ,是决定有氧能力和训练敏感性的可能分子机制之一。展示了中国运动员和汉人 m t DNA HVR 多态性 ,及其与运动能力关联性研究的结果 ,并对比分析了限制性酶切、直接测序、活细胞培养等方法对 mt DNA多态性与运动能力的研究结果 ,发现众多研究结果还存在不少争议 ,仍需进一步深入研究。 m t DNA作为良好的遗传标记 ,对其单核苷酸多态性(SNP)的分析及其与运动能力表型的关联研究和分子遗传学探讨具有重要意义和研究前景。
文摘The cotton bollworm Helicoverpa armigera and the oriental tobacco budworm H. assulta are sibling species, both being important agricultural pests. Morphologically, the two insects are almost indistinguishable at the egg, larval and pupal stages. One of the big challenges in the study of these insects, in particular in integrated pest management, is a timely and dependable identification of these insects at their early stages of development. Here, we report a H. armigera-specific nuclear DNA marker, and demonstrate that it can be employed to reliably discriminate between 1-1. armigera and 11. assulta by simple polymerase chain reaction amplification experiment.
