Nucleic acid nanotechnology has been developed to be a promising strategy to construct various nano-biomaterials with structural programmability, spatial addressability, and excellent biocompatibility. Self-assembled ...Nucleic acid nanotechnology has been developed to be a promising strategy to construct various nano-biomaterials with structural programmability, spatial addressability, and excellent biocompatibility. Self-assembled nucleic acid nanostructures have been employed in a variety of biomedical applications, such as bio-imaging, diagnosis, and therapeutics. In this manuscript, we will review recent progress in the development of multifunctional nucleic acid nanostructures as gene drug delivery vehicles. Therapeutic systems based on RNA interference (RNAi), clustered regularly interspaced short palindromic repeat associated proteins 9 system (CRISPR/Cas9) genome editing, gene expression, and CpG-based immunostimulation will be highlighted. We will also discuss the challenges and future directions of nucleic acid nanotechnology in biomedical research.展开更多
Nucleic acid nanostructures with structural programmability, spatial addressability and excellent biocompatibility have drawn much attention in various biomedical applications, such as bioimaging, biosensing and drug ...Nucleic acid nanostructures with structural programmability, spatial addressability and excellent biocompatibility have drawn much attention in various biomedical applications, such as bioimaging, biosensing and drug delivery. In this review, we summarize the recent research progress in the field of bioimaging based on nucleic acid nanostructures with different imaging models, including fluorescent imaging(FI), magnetic resonance imaging(MRI), photoacoustic imaging(PAI) and positron emission tomography/computed tomography(PET/CT) imaging. We also discuss the remaining challenges and further opportunities involved in the bioimaging research based on nucleic acid nanostructures.展开更多
Photothermal therapy is a direct and non-invasive therapeutic strategy.Herein,we report a general strategy for the construction of polyaniline-coated DNA nanoparticles for efficient photothermal therapy.In our design,...Photothermal therapy is a direct and non-invasive therapeutic strategy.Herein,we report a general strategy for the construction of polyaniline-coated DNA nanoparticles for efficient photothermal therapy.In our design,two G4/hemin-based DNAzymes are precisely organized in a DNA template to mimic the function of horseradish peroxidase(HRP)for the catalytic oxidation of aniline.After polymerization,the polyaniline-coated DNA nanoparticles(PANI/DNA NPs)can be efficiently obtained as the photothermal agent to elicit a pronounced photothermal effect.After cellular uptake,the noticeable photothermal effect from 808 nm laser irradiation can achieve an efficient proliferation inhibition of tumor cells.This rationally developed photothermal agent based on DNA template-assisted polymerization presents a new avenue for the development of photothermal therapy.展开更多
基金This work is supported by the National Natural Science Foundation of China (Nos. 21573051, 21708004, and 51761145044), Sience Fund of Creative Research Groups of the National Natural Science Foundation of China (No. 21721002), the National Basic Research Program of China (No. 2016YFA0201601), Beijing Municipal Science & Technology Commission (No. Z161100000116036), Key Research Program of Frontier Sciences, CAS, Grant QYZDB-SSW-SLH029, CAS Interdisciplinary Innovation Team, and K. C. Wong Education Foundation.
文摘Nucleic acid nanotechnology has been developed to be a promising strategy to construct various nano-biomaterials with structural programmability, spatial addressability, and excellent biocompatibility. Self-assembled nucleic acid nanostructures have been employed in a variety of biomedical applications, such as bio-imaging, diagnosis, and therapeutics. In this manuscript, we will review recent progress in the development of multifunctional nucleic acid nanostructures as gene drug delivery vehicles. Therapeutic systems based on RNA interference (RNAi), clustered regularly interspaced short palindromic repeat associated proteins 9 system (CRISPR/Cas9) genome editing, gene expression, and CpG-based immunostimulation will be highlighted. We will also discuss the challenges and future directions of nucleic acid nanotechnology in biomedical research.
基金This work was supported by the National Natural Science Foundation of China(Nos.22025201,22077023 and 21721002)the National Basic Research Program of China(Nos.2016YFA0201601 and 2018YFA0208900)+3 种基金the Fund of the Beijing Municipal Science&Technology Commission,China(No.Z191100004819008)the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000)the Key Research Program of Frontier Sciences of CAS(No.QYZDB-SSW-SLH029)the CAS Interdisciplinary Innovation Team,Youth Innovation Promotion Association CAS and K.C.Wong Education Foundation(No.GJTD-2018-03).
文摘Nucleic acid nanostructures with structural programmability, spatial addressability and excellent biocompatibility have drawn much attention in various biomedical applications, such as bioimaging, biosensing and drug delivery. In this review, we summarize the recent research progress in the field of bioimaging based on nucleic acid nanostructures with different imaging models, including fluorescent imaging(FI), magnetic resonance imaging(MRI), photoacoustic imaging(PAI) and positron emission tomography/computed tomography(PET/CT) imaging. We also discuss the remaining challenges and further opportunities involved in the bioimaging research based on nucleic acid nanostructures.
基金supported by the National Key R&D Program of China(Nos.2021YFA1200302,2018YFA0208900)the National Natural Science Foundation of China(Nos.22025201,22077023,21721002)+2 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000)the CAS Project for Young Scientists in Basic Research(No.YSBR-036)the CAS Interdisciplinary Innovation Team,Youth Innovation Promotion Association CAS,and K.C.Wong Education Foundation(No.GJTD-2018-03).
文摘Photothermal therapy is a direct and non-invasive therapeutic strategy.Herein,we report a general strategy for the construction of polyaniline-coated DNA nanoparticles for efficient photothermal therapy.In our design,two G4/hemin-based DNAzymes are precisely organized in a DNA template to mimic the function of horseradish peroxidase(HRP)for the catalytic oxidation of aniline.After polymerization,the polyaniline-coated DNA nanoparticles(PANI/DNA NPs)can be efficiently obtained as the photothermal agent to elicit a pronounced photothermal effect.After cellular uptake,the noticeable photothermal effect from 808 nm laser irradiation can achieve an efficient proliferation inhibition of tumor cells.This rationally developed photothermal agent based on DNA template-assisted polymerization presents a new avenue for the development of photothermal therapy.