Detection of nucleoside derivatives has paramount importance because they are the essential biomolec-ular units for all life.Herein,we report a host-guest approach by using a fluorescent tetraphenylethene-based octaca...Detection of nucleoside derivatives has paramount importance because they are the essential biomolec-ular units for all life.Herein,we report a host-guest approach by using a fluorescent tetraphenylethene-based octacationic cage as host and 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt(HPTS)as guest and fluorescent indicator to form non-fluorescent 1:1:1 host-(endo-exo)guest complex in water.This new host-(endo-exo)guest complex can be successfully used for detecting nucleosides(e.g.,ATP and GTP),DNA(e.g.,sm-DNA),and antibiotics(e.g.,Penicillin G)with off-on fluorescence response via a competitive host-guest exchange with HPTS as exo-guest in water.Furthermore,this on-off-on fluorescent host-guest com-plex is also used for cell imaging based on ATP concentration in HeLa cells.Therefore,this study not only provides insight into the construction of a supramolecular probe with on-off-on fluorescence via host-guest complexation and exchange in solution,but also realizes a universal method for detecting and monitoring biomolecules.展开更多
基金supported by the National Natural Science Foun-dation of China(Nos.22122108,21971208 and 21771145)the Natu-ral Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China(No.2021JC-37)the Fok Ying Tong Education Foundation(No.171010).
文摘Detection of nucleoside derivatives has paramount importance because they are the essential biomolec-ular units for all life.Herein,we report a host-guest approach by using a fluorescent tetraphenylethene-based octacationic cage as host and 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt(HPTS)as guest and fluorescent indicator to form non-fluorescent 1:1:1 host-(endo-exo)guest complex in water.This new host-(endo-exo)guest complex can be successfully used for detecting nucleosides(e.g.,ATP and GTP),DNA(e.g.,sm-DNA),and antibiotics(e.g.,Penicillin G)with off-on fluorescence response via a competitive host-guest exchange with HPTS as exo-guest in water.Furthermore,this on-off-on fluorescent host-guest com-plex is also used for cell imaging based on ATP concentration in HeLa cells.Therefore,this study not only provides insight into the construction of a supramolecular probe with on-off-on fluorescence via host-guest complexation and exchange in solution,but also realizes a universal method for detecting and monitoring biomolecules.
