Benzo[a]pyrene(B[a]P) is an increasingly present marine environmental pollutant,yet our understanding of the long-term consequences of reproductive toxicity in marine benthic polychaetes remains limited.To test the re...Benzo[a]pyrene(B[a]P) is an increasingly present marine environmental pollutant,yet our understanding of the long-term consequences of reproductive toxicity in marine benthic polychaetes remains limited.To test the reproductive toxicity of B[a]P on polychaetes,Perinereis nuntia was exposed to B[a]P-contaminated artificial seawater and sexual maturation,the sex ratio,number of eggs spawned,fertilization and hatching rated,as well as vitellogenin(VTG) mRNA expression levels were analyzed.A low concentration of B[a]P(2.5μg/L) had no effects on the rate of sexual maturation,spawning,or fertilization but significantly increased the sex ratio(female:male) from 1.6±0.15:1 to 2.3±0.18:1,inhibited hatching rate by 27%,and significantly increased VTG mRNA expression level by 3.7-fold following a60-day exposure,compared with those in the solvent controls.A higher concentration of B[a]P(25μg/L)caused more serious effects;sexual maturation,fertilization success,and hatching decreased by 31%,17% and 46%,respectively,and the sex ratio(female:male) and VTG mRNA gene expression level increased by54% and 7.1-fold,respectively.These results demonstrate that sublethal concentrations of B[a]P negatively affect reproductive performance of the sandworm P.nuntia.展开更多
基金Supported by the Guangdong High-Level University Project"Green Technologies for Marine Industries"(No.130-33106703)Guangdong Natural Science Foundation(2016A030313065)the Outstanding Young Teachers of Guangdong Colleges and Universities(No.YQ2015074)
文摘Benzo[a]pyrene(B[a]P) is an increasingly present marine environmental pollutant,yet our understanding of the long-term consequences of reproductive toxicity in marine benthic polychaetes remains limited.To test the reproductive toxicity of B[a]P on polychaetes,Perinereis nuntia was exposed to B[a]P-contaminated artificial seawater and sexual maturation,the sex ratio,number of eggs spawned,fertilization and hatching rated,as well as vitellogenin(VTG) mRNA expression levels were analyzed.A low concentration of B[a]P(2.5μg/L) had no effects on the rate of sexual maturation,spawning,or fertilization but significantly increased the sex ratio(female:male) from 1.6±0.15:1 to 2.3±0.18:1,inhibited hatching rate by 27%,and significantly increased VTG mRNA expression level by 3.7-fold following a60-day exposure,compared with those in the solvent controls.A higher concentration of B[a]P(25μg/L)caused more serious effects;sexual maturation,fertilization success,and hatching decreased by 31%,17% and 46%,respectively,and the sex ratio(female:male) and VTG mRNA gene expression level increased by54% and 7.1-fold,respectively.These results demonstrate that sublethal concentrations of B[a]P negatively affect reproductive performance of the sandworm P.nuntia.