Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of...Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of the gene expression profiles of the body walls of A. japonicus exposed to strong light（＂light＂）, normal light（＂control＂） and fully dark（＂dark＂） environment. In the comparisons of ＂control＂ vs. ＂dark＂, ＂control＂ vs. ＂light＂ and ＂dark＂ vs.＂light＂, 1 161, 113 and 1 705 differentially expressed genes（DEGs） were identified following the criteria of｜log2 ratio｜≥1 and FDR≤0.001, respectively. Gene ontology analysis showed that ＂cellular process＂ and ＂binding＂enriched the most DEGs in the category of ＂biological process＂ and ＂molecular function＂, while ＂cell＂ and ＂cell part＂ enriched the most DEGs in the category of ＂cellular component＂. And the DEGs were mapped to 214, 41 and229 pathways in the Kyoto Encyclopedia of Genes and Genomes database, and 51, 2 and 57 pathways were significantly enriched, respectively. Light-specific DEGs identified in this study will be important targets for further investigation to establish the biochemical mechanisms involved in the adaption of this sea cucumber to changes in the level of environmental light.展开更多
为研究刺参热加工过程中体壁胶原蛋白的物性变化规律,利用原子力显微镜(atomic force microscopy,AFM)考察样品浓度、溶剂和热处理条件对刺参体壁酶促溶性胶原蛋白(pepsin-soluble collagen,PSC)溶液聚集状态的影响。结果表明:在乙酸缓...为研究刺参热加工过程中体壁胶原蛋白的物性变化规律,利用原子力显微镜(atomic force microscopy,AFM)考察样品浓度、溶剂和热处理条件对刺参体壁酶促溶性胶原蛋白(pepsin-soluble collagen,PSC)溶液聚集状态的影响。结果表明:在乙酸缓冲液(pH2.7)、PBS缓冲液(pH7.2)和超纯水中,海参体壁酶促溶性胶原蛋白均随着浓度的增大而逐渐开始自组装,并最终形成无规则的团状结构或者片状网络结构。但是,溶剂不同,其开始自组装所需要的胶原蛋白浓度也不同。随着加热温度的升高或加热时间的延长,刺参体壁酶促溶性胶原蛋白分子逐渐聚集成网络结构,但加热温度超过100℃或时间超过2 h后,其交联程度逐渐降低,网络结构逐渐消失。展开更多
基金The National Natural Science Foundation of China under contract No.41676136the Strategic Priority Research Program of the Chinese Academy of Sciences under contract No.XDA11020703the Science and Technology Major Project of Shandong Province(Industry Transformation and Upgrading) under contract No.2015ZDZX05002
文摘Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of the gene expression profiles of the body walls of A. japonicus exposed to strong light（＂light＂）, normal light（＂control＂） and fully dark（＂dark＂） environment. In the comparisons of ＂control＂ vs. ＂dark＂, ＂control＂ vs. ＂light＂ and ＂dark＂ vs.＂light＂, 1 161, 113 and 1 705 differentially expressed genes（DEGs） were identified following the criteria of｜log2 ratio｜≥1 and FDR≤0.001, respectively. Gene ontology analysis showed that ＂cellular process＂ and ＂binding＂enriched the most DEGs in the category of ＂biological process＂ and ＂molecular function＂, while ＂cell＂ and ＂cell part＂ enriched the most DEGs in the category of ＂cellular component＂. And the DEGs were mapped to 214, 41 and229 pathways in the Kyoto Encyclopedia of Genes and Genomes database, and 51, 2 and 57 pathways were significantly enriched, respectively. Light-specific DEGs identified in this study will be important targets for further investigation to establish the biochemical mechanisms involved in the adaption of this sea cucumber to changes in the level of environmental light.
文摘为研究刺参热加工过程中体壁胶原蛋白的物性变化规律,利用原子力显微镜(atomic force microscopy,AFM)考察样品浓度、溶剂和热处理条件对刺参体壁酶促溶性胶原蛋白(pepsin-soluble collagen,PSC)溶液聚集状态的影响。结果表明:在乙酸缓冲液(pH2.7)、PBS缓冲液(pH7.2)和超纯水中,海参体壁酶促溶性胶原蛋白均随着浓度的增大而逐渐开始自组装,并最终形成无规则的团状结构或者片状网络结构。但是,溶剂不同,其开始自组装所需要的胶原蛋白浓度也不同。随着加热温度的升高或加热时间的延长,刺参体壁酶促溶性胶原蛋白分子逐渐聚集成网络结构,但加热温度超过100℃或时间超过2 h后,其交联程度逐渐降低,网络结构逐渐消失。
