The application of phenylfluorone (PF)-Mo(VI) complex as a spectroscopic probe is studied. In the presence of OP microemulsion at pH 3.04, PF-Mo(Ⅵ) complex combines protein rapidly to form a stable compound and the a...The application of phenylfluorone (PF)-Mo(VI) complex as a spectroscopic probe is studied. In the presence of OP microemulsion at pH 3.04, PF-Mo(Ⅵ) complex combines protein rapidly to form a stable compound and the absorbance at 527 nm is in proportion to the concentration of protein in the range 0-16 μg mL-1 for bovine serum albumin (BSA). OP microemuslion media is introduced into protein determination, it has increased markedly the sensitivity of the system. The molar absorption coefficient was 5.98×l06 L mol-1 cm-1 for BSA. The assay, with sensitivity, simplicity and tolerance to many foreign substances, is applied to the determination of protein in samples with satisfactory results. Moreover, the binding number of BSA with the complex, which is determined by molar ratio and slope ratio methods, is in good agreement.展开更多
Cis-dioxo-tungsten(Ⅵ ) complex, (NH2CH2CH2NH2)[(NH2CH2CH2NH3)2WⅥO2(NHC6H4NH)2]2·H2O is synthesized at room temperature by the reaction of sodium tungstate with o-phenylenediamine. The crystal structure of compl...Cis-dioxo-tungsten(Ⅵ ) complex, (NH2CH2CH2NH2)[(NH2CH2CH2NH3)2WⅥO2(NHC6H4NH)2]2·H2O is synthesized at room temperature by the reaction of sodium tungstate with o-phenylenediamine. The crystal structure of complex was determined by X-ray diffraction structural analysis. The results show that complex belongs to monoclinic system with space group P21 / c, a=0.712 8(2) nm, b=3.081 1(10) nm, c=0.981 9(3) nm, β=102.615(4)°, V=2.104 4(11) nm3, Z=2, μ=55.26 cm-1, F(000)=1 176. Compared the complex with its analogous biomimetic complexes of the cofactor of molybdoenzymes and tungstoenzymses, it is found that the variance of the coordination atoms and the metal ions center have no influence on the coordination feature, and exhibits distored octahedral coordination with cis-dioxo o-phenylenediamine. CCDC: 252834.展开更多
Uranium(Ⅵ) complex [UO2((R,S)-1,1′-binaphthylene-2,2′-dicarboxylate)(H2O)] was obtained by thehydrothermal treatment of UO2(NO3)2.6H2O with(R,S)-1,1′-binaphthylene-2,2′-dicarboxylic acid(BCA)(L) in water at 180 ...Uranium(Ⅵ) complex [UO2((R,S)-1,1′-binaphthylene-2,2′-dicarboxylate)(H2O)] was obtained by thehydrothermal treatment of UO2(NO3)2.6H2O with(R,S)-1,1′-binaphthylene-2,2′-dicarboxylic acid(BCA)(L) in water at 180 ℃ in Pyrex tube.The crystal belongs to monoclinic system,space group C2/c,with a=1.640 3(3) nm,b= 1.1967(2) nm,c=1.0663(17) nm,β=104.412(4)°,V=2.0272(6) nm3,Z=4.CCDC: 659617.展开更多
文摘The application of phenylfluorone (PF)-Mo(VI) complex as a spectroscopic probe is studied. In the presence of OP microemulsion at pH 3.04, PF-Mo(Ⅵ) complex combines protein rapidly to form a stable compound and the absorbance at 527 nm is in proportion to the concentration of protein in the range 0-16 μg mL-1 for bovine serum albumin (BSA). OP microemuslion media is introduced into protein determination, it has increased markedly the sensitivity of the system. The molar absorption coefficient was 5.98×l06 L mol-1 cm-1 for BSA. The assay, with sensitivity, simplicity and tolerance to many foreign substances, is applied to the determination of protein in samples with satisfactory results. Moreover, the binding number of BSA with the complex, which is determined by molar ratio and slope ratio methods, is in good agreement.
文摘Cis-dioxo-tungsten(Ⅵ ) complex, (NH2CH2CH2NH2)[(NH2CH2CH2NH3)2WⅥO2(NHC6H4NH)2]2·H2O is synthesized at room temperature by the reaction of sodium tungstate with o-phenylenediamine. The crystal structure of complex was determined by X-ray diffraction structural analysis. The results show that complex belongs to monoclinic system with space group P21 / c, a=0.712 8(2) nm, b=3.081 1(10) nm, c=0.981 9(3) nm, β=102.615(4)°, V=2.104 4(11) nm3, Z=2, μ=55.26 cm-1, F(000)=1 176. Compared the complex with its analogous biomimetic complexes of the cofactor of molybdoenzymes and tungstoenzymses, it is found that the variance of the coordination atoms and the metal ions center have no influence on the coordination feature, and exhibits distored octahedral coordination with cis-dioxo o-phenylenediamine. CCDC: 252834.
文摘Uranium(Ⅵ) complex [UO2((R,S)-1,1′-binaphthylene-2,2′-dicarboxylate)(H2O)] was obtained by thehydrothermal treatment of UO2(NO3)2.6H2O with(R,S)-1,1′-binaphthylene-2,2′-dicarboxylic acid(BCA)(L) in water at 180 ℃ in Pyrex tube.The crystal belongs to monoclinic system,space group C2/c,with a=1.640 3(3) nm,b= 1.1967(2) nm,c=1.0663(17) nm,β=104.412(4)°,V=2.0272(6) nm3,Z=4.CCDC: 659617.
