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Antioxidant and Pasting Properties of Oat <i>β</i>-Glucan Hydrocolloids
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作者 George E. Inglett Diejun Chen 《Food and Nutrition Sciences》 2012年第6期827-835,共9页
Four oat β-glucan enriched hydrocolloids (Nutrim10, C-Trim20, C-Trim30, C-Trim50), oat bran concentrate (OBC), and β-Glucan95 were investigated for antioxidant and pasting properties. C-Trim30 had the highest solubl... Four oat β-glucan enriched hydrocolloids (Nutrim10, C-Trim20, C-Trim30, C-Trim50), oat bran concentrate (OBC), and β-Glucan95 were investigated for antioxidant and pasting properties. C-Trim30 had the highest soluble phenolic content, followed by C-Trim20. The trend of antioxidant activity was similar with that of phenolic contents. The phenolic content of the extracts increased with increasing temperatures. The highest content of soluble phenolic compounds was found at temperatures up to 100℃ for most samples regardless of solvent. Water extracts had significantly higher phenolic contents than extracts from 50% ethanol at 100℃ for all samples with the exception of C-Trim30. However, the effect of temperature and solvent concentrations was not as apparent for antioxidant activity as that observed for phenolic content. In general, the differences in three different solvents were not as apparent. Significantly higher water holding capacities were found for C-Trim30 and C-Trim50 than the other samples while β-Glucan 95 had substantially the highest paste viscosity followed by C-Trim50 and C-Trim30. 展开更多
关键词 oat β-glucan HYDROCOLLOIDS PASTING Properties ANTIOXIDANT Activity
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Application of naturalβ-glucans as biocompatible functional nanomaterials
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作者 Xiaojie Li Peter Chi Keung Cheung 《Food Science and Human Wellness》 SCIE 2019年第4期315-319,共5页
Naturally-occurringβ-glucans are mostly investigated for their antitumor activity and immunomodulatory property.They have been widely regarded as a natural source for functional foods and pharmaceuticals.However,the ... Naturally-occurringβ-glucans are mostly investigated for their antitumor activity and immunomodulatory property.They have been widely regarded as a natural source for functional foods and pharmaceuticals.However,the physico-chemically stable and biocompatible properties ofβ-glucans are rarely explored as a coating material for nanomaterials to overcome the problems of aggregation and cytotoxicity.This article reviews on the exploration ofβ-glucans,in particular those derived from mushrooms,as a natural coating material to modify the surface properties of bioactive substances as a relatively simple and cost-effective strategy to produce stable and biocompatible nanohybrids used for biopharmaceutical use.It is envisaged that suchβ-glucan-based coating method will provide new opportunities to design biocompatible functional nanomaterials for wider clinical applications. 展开更多
关键词 β-glucans BIOCOMPATIBILITY BIOPHARMACEUTICALS NANOHYBRIDS
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Quality and Sensory Properties of Reduced Fat Yoghurt Made with Addition of <i>β</i>-Glucans
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作者 Eleni C. Pappa Efthymia Kondyli +3 位作者 William MacNaughtan Athanasia Kakouri Konstantinos Nesseris Cleanthes Israilides 《Food and Nutrition Sciences》 2018年第4期390-402,共13页
β-Glucans, isolated from Pleurotus citrinopileatus mushroom, were used in the manufacture of yoghurt from reduced fat cow milk with added proteins. β-Glucans were added to the milk in the form of a paste containing ... β-Glucans, isolated from Pleurotus citrinopileatus mushroom, were used in the manufacture of yoghurt from reduced fat cow milk with added proteins. β-Glucans were added to the milk in the form of a paste containing 1.21% (on a dry weight basis) of β-glucans, such that the final concentrations in the yoghurt were 0.3%, 0.4% and 0.5%. Control yoghurt was also manufactured from the same milk without the addition of β-glucans. The resultant yoghurts were examined for their physicochemical and microbial characteristics, gel formation and sensory properties and compared to the control at 1, 7, 14 and 21 days of storage. Results showed that the microbial population, pH, titratable acidity, ash and syneresis were not affected by the addition of different levels of glucans. The lowest fat, total solids and protein contents were recorded in the yoghurt containing 0.5% glucan. The yoghurt containing 0.3% β-glucan had the same viscosity as the control and the yoghurt containing 0.5% β-glucan had the lowest viscosity. Yoghurt with β-glucans received slightly higher scores for flavour, appearance and texture than the control. It was concluded that the incorporation of 0.3% or 0.4% β-glucans isolated from edible mushrooms, into the yoghurt does not affect the final product, which is similar or sensorially even better than the control. 展开更多
关键词 β-glucans Functional YOGHURT PLEUROTUS citrinopileatus MUSHROOM
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A Review on the Impact of the Plant Bioactive Compound β-Glucans as Phytochemicals for Boosting Human and Animals’ Immune Response—β-Glucans as Immunostimulants
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作者 Mirjana Menkovska Osama Ibrahim 《Advances in Bioscience and Biotechnology》 CAS 2022年第7期298-315,共18页
The influence of food and nutrition on health and immunity for both human and animals are being demonstrated. Phytochemicals are natural compounds present in cereals, beans, fruits, vegetable, and other plants, believ... The influence of food and nutrition on health and immunity for both human and animals are being demonstrated. Phytochemicals are natural compounds present in cereals, beans, fruits, vegetable, and other plants, believed to enhance immune response for both human and animals. One of these phytochemicals is β-Glucans that are heterogeneous polysaccharides consisted of branched long chains of D-Glucose units present in cereals such as barley, wheat, and oats, and also present in microbial cell walls for yeasts, fungi, bacteria, and algae. β-Glucans extracted from cereals and mushroom were investigated for their positive impact on immunomodulation for both human and animal health. These β-Glucans proved to enhance immune system for innate response as the first defense against microbial infection, toxins, and self-tumor cells, and also, enhance adaptive immune that also referred to specific immunity as the second defense in response to antigen-specific lymphocytes against microbial infection, toxins, and self-tumor cells. 展开更多
关键词 β-glucans Source Structure Properties IMMUNOMODULATION
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β-Glucans: Characterization, Extraction Methods, and Valorization
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作者 Ana Chioru Aurica Chirsanova 《Food and Nutrition Sciences》 2023年第10期963-983,共21页
β-glucans are bioactive compounds with a wide range of biological properties, including anticancer, anti-inflammatory, antioxidant, and immune-modulating properties. Due to their specific physical properties, such as... β-glucans are bioactive compounds with a wide range of biological properties, including anticancer, anti-inflammatory, antioxidant, and immune-modulating properties. Due to their specific physical properties, such as (in)solubility, viscosity, and gelation, β-glucans are increasingly being used in the food, pharmaceutical, and cosmetic industries. The purpose of this review is to provide an overview of the different types of β-glucans, their sources, especially Saccharomyces cerevisiae yeasts, and the methods of extraction, isolation, and purification of β-glucans, with the aim of optimizing these methods for the efficient production process. Moreover, the physico-chemical properties, modifications, current applications and future prospects of the use of β-glucans in food, medicines, cosmetics and other potential value-added products are summarized. The data presented indicate that β-glucans will play an increasingly important role in the sector of special-purpose food products as well as in other current and future areas. 展开更多
关键词 β-glucans YEAST EXTRACTION VALORIZATION Value-Added Products
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Effects of β-Glucan Supplementation on Repairing of Phenol-Induced Vaginal Mucosal Epithelium Damage in Rats
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作者 Song Fei Weidong Wu +2 位作者 Ying Wang Dan Li Bo Jin 《Open Journal of Obstetrics and Gynecology》 2024年第4期535-546,共12页
Objective: To investigate the effects of different concentrations of β-glucan on the repair of damaged vaginal mucosa, the expression of vascular endothelial growth factor (VEGF), and the inflammatory factor-6 (IL-6)... Objective: To investigate the effects of different concentrations of β-glucan on the repair of damaged vaginal mucosa, the expression of vascular endothelial growth factor (VEGF), and the inflammatory factor-6 (IL-6) in vaginal tissues. Methods: Thirty-six adult female specific pathogen free (SPF)-grade Wistar rats were randomly divided into 3 phase groups with 12 rats each. Vaginal inflammation rat models were established by injecting phenol gel into the vagina of each rat at a dose of 0.1 ml/100g body weight. After modeling, rats were divided into 4 groups based on different concentrations of the test agent. The control group was injected with 0.5 ml of saline, experimental group A was injected with 0.375 ml saline 0.125 ml β-glucan, experimental group B was injected with 0.25 ml saline 0.25 ml β-glucan, and experimental group C was injected with 0.50 ml β-glucan. The injection sites were selected at the 3 o’clock and 9 o’clock positions of the vagina. Rats were sacrificed at 7-, 14-, and 28-days post-injection, and tissue samples were collected from the injection sites and prepared for histological analysis. New blood vessels and fibroblast numbers in the tissues were observed after Hematoxylin-eosin (HE) staining. The expression levels of VEGF and IL-6 in the tissues were measured using quantificational reverse transcription polymerase chain reaction (qRT-PCR). Results: Histological examination of vaginal tissue specimens at 7-, 14-, and 28-days post-injection showed that on day 7, there were no significant changes in the experimental groups compared to the control group. However, on days 14 and 28, the experimental groups showed more new blood vessels, macrophages, and fibroblasts with increased activity compared to the control group. The expression levels of VEGF in vaginal tissues were elevated on days 14 and 28 in the experimental groups. The comparison of IL-6 levels in vaginal tissues on day 28 showed that serum IL-6 levels returned to normal, and there was no statistically significant difference between the experimental and control groups. Conclusion: In the 3 experimental phases, the increase in VEGF levels in vaginal tissues on day 14 post-injection was more pronounced with higher concentrations of β-glucan, and IL-6 levels returned to normal on day 28. β-Glucan can enhance VEGF levels in damaged vaginal tissues, promote the repair of damaged vaginal tissues, and higher concentrations of β-glucan have a better effect. 展开更多
关键词 Β-glucan Vaginal Mucosa Damage Repair
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普通烟草鸟氨酸转氨酶基因Ntδ-OAT的克隆与表达分析 被引量:3
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作者 焦蓉 刘贯山 +7 位作者 刘好宝 王树林 侯娜 王全贞 靳义荣 刘朝科 冯祥国 胡晓明 《植物遗传资源学报》 CAS CSCD 北大核心 2011年第4期588-593,600,共7页
鸟氨酸转氨酶是以鸟氨酸为前体合成脯氨酸的关键酶,对植物适应逆境胁迫起关键作用。本研究根据其已知保守序列设计兼并引物,从普通烟草品种中烟14中克隆到一条425bp的中间片段,应用RACE方法设计特异性引物扩增得到5′末端和3′末端cDNA... 鸟氨酸转氨酶是以鸟氨酸为前体合成脯氨酸的关键酶,对植物适应逆境胁迫起关键作用。本研究根据其已知保守序列设计兼并引物,从普通烟草品种中烟14中克隆到一条425bp的中间片段,应用RACE方法设计特异性引物扩增得到5′末端和3′末端cDNA序列,经BLAST验证,首次从普通烟草中克隆到了鸟氨酸转氨酶基因,命名为Ntδ-OAT,其GenBank登录号为GU144571。该基因cDNA全长为1781bp,共编码477个氨基酸。系统进化树分析显示,Ntδ-OAT基因的进化符合传统的生物学分类,主要功能域在生物进化中保守性较高。Real-time PCR分析表明,Ntδ-OAT基因受干旱、高盐、低温、ABA等多种胁迫处理的诱导表达,可能参与普通烟草体内抗渗透胁迫过程。 展开更多
关键词 普通烟草 鸟氨酸转氨酶 Nt-δoat 克隆 表达分析
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菊苣对高尿酸血症鹌鹑肾脏有机阴离子转运体OAT3-LIKE的影响 被引量:8
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作者 李丽玉 林志健 +5 位作者 张冰 王雪洁 朱春胜 牛红娟 周月 董百惠 《中药新药与临床药理》 CAS CSCD 北大核心 2015年第3期284-289,共6页
目的从肾脏转运蛋白角度探究中药菊苣降尿酸的药效机制。方法将40只雄性迪法克鹌鹑按体质量随机分为5组,正常组给予普通饲料,其余各组均给予含酵母饲料(15 g·kg-1·d-1)建立高尿酸血症模型,同时给药组分别给予菊苣5,7.5 g·... 目的从肾脏转运蛋白角度探究中药菊苣降尿酸的药效机制。方法将40只雄性迪法克鹌鹑按体质量随机分为5组,正常组给予普通饲料,其余各组均给予含酵母饲料(15 g·kg-1·d-1)建立高尿酸血症模型,同时给药组分别给予菊苣5,7.5 g·kg-1·d-1,苯溴马隆20 mg·kg-1·d-1,实验第35天处死。检测鹌鹑血清尿酸(Uric Acid,UA)、尿素氮(Urea Nitrogen,BUN)水平,并用Real-time RT-PCR(Real-time Reverse TranscriptionPolymerase Chain Reaction)方法检测其肾脏有机阴离子转运体OAT3-LIKE(Organic Anion Transporter3-LIKE)的mRNA表达水平,同时测定肝脏尿酸代谢相关酶活性。结果与正常组比较,实验第21天、28天、35天模型组鹌鹑血尿酸升高,第35天肾脏OAT3-LIKE m RNA表达水平下降。与模型组比较,实验第35天,菊苣可以降低鹌鹑血尿酸水平,上调其肾脏OAT3-LIKE mRNA表达水平并降低肝脏腺苷脱氨酶(ADA)活性。结论高嘌呤食饵可以成功诱导鹌鹑高尿酸血症;菊苣具有抑制高尿酸血症鹌鹑尿酸生成和促进尿酸排泄的双重作用;菊苣药效机制可能与降低ADA活性及上调OAT3-LIKE表达水平相关。 展开更多
关键词 菊苣 鹌鹑 高尿酸血症 肾脏有机阴离子转运体 oat3-LIKE
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OAT三维度科技查新质量评价指标体系的构建 被引量:2
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作者 严栋 顾立志 +1 位作者 李凡 季叶克 《四川图书馆学报》 2016年第6期36-39,共4页
提出采用客观性维度、资质维度和时效性维度建构科技查新质量评价指标与体系,并以模糊算法量化质量评价指标,提出科技查新质量评价模型。查新实践表明该模型能够描述和评价科技查新质量,且具有较好的置信度。
关键词 oat三维度 科技查新 质量评价指标
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Application of Oats to Control the Weeds in the Seedling Period of Alfalfa Sowed in Spring 被引量:2
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作者 郭芸江 张丽 唐华 《Agricultural Science & Technology》 CAS 2011年第8期1201-1205,共5页
[Objective]The aim was to solve the weeds existed in seedling period of alfalfa in Chongqing.[Method]The naked oat and common oat were selected.Through the analysis of determiners such as plant number,plant height,yie... [Objective]The aim was to solve the weeds existed in seedling period of alfalfa in Chongqing.[Method]The naked oat and common oat were selected.Through the analysis of determiners such as plant number,plant height,yield and weeds ratio,the oat type and sowing method were screened through all the accompany sowing crops to better control weeds in the seedling period of alfalfa.[Result]Compared with common oat,the tested naked oat as the companion crop of alfalfa seeding can prevent weeds more effectively;the proper companion-seeding disposal of alfalfa sowed in spring in Chongqing area was 15 kg/hm2 of alfalfa and 120 kg/hm2 of naked oat,sowing in strip in 40 cm row spacing;the proper mowing period of oat was during the time from May 11th to May 21st;the proper mowing period of alfalfa was ten days later.[Conclusion]Spring sowing oats with alfalfa can control seedling weeds effectively. 展开更多
关键词 ALFALFA oat Slligator alternenthera Companion crop
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健脾渗湿方对高尿酸血症大鼠模型尿酸盐转运蛋白OAT3调控作用的研究 被引量:15
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作者 吴生元 彭江云 +2 位作者 万春平 李兆福 杨会军 《风湿病与关节炎》 2013年第12期22-25,共4页
目的:研究健脾渗湿方对高尿酸血症大鼠模型尿酸盐转运蛋白OAT3表达的影响,揭示其治疗高尿酸血症的作用机制。方法:40只大鼠随机分为正常对照组、模型对照组、苯溴马隆组和健脾渗湿方组,每组10只。采用酵母饲料加腺嘌呤诱导高尿酸血症大... 目的:研究健脾渗湿方对高尿酸血症大鼠模型尿酸盐转运蛋白OAT3表达的影响,揭示其治疗高尿酸血症的作用机制。方法:40只大鼠随机分为正常对照组、模型对照组、苯溴马隆组和健脾渗湿方组,每组10只。采用酵母饲料加腺嘌呤诱导高尿酸血症大鼠模型,灌胃给药14 d后,检测高尿酸血症大鼠肾脏病理损伤,免疫组化法检测尿酸盐转运蛋白OAT3的表达情况。结果:健脾渗湿方组高尿酸血症模型大鼠肾脏的损伤程度显著改善,且对尿酸盐转运蛋白OAT3具有显著的上调作用。结论:上调尿酸盐转运蛋白OAT3,可能是健脾渗湿法治疗高尿酸血症的作用机制之一。 展开更多
关键词 痛风 高尿酸血症 oat3 健脾渗湿方 大鼠
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对象的交互测试与OATS正交阵列测试系统 被引量:2
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作者 柴小卉 《计算机工程与应用》 CSCD 北大核心 2004年第15期82-84,共3页
对象交互测试是面向对象软件测试的一个重要环节。交互测试的重点是确保对象的消息能够正确传送。文章在完全测试不可能的情况下,提供了一种特殊的抽样方法———正交阵列测试方法OATS,从统计学角度指导“实验”以测试组件所有可能配置... 对象交互测试是面向对象软件测试的一个重要环节。交互测试的重点是确保对象的消息能够正确传送。文章在完全测试不可能的情况下,提供了一种特殊的抽样方法———正交阵列测试方法OATS,从统计学角度指导“实验”以测试组件所有可能配置的子集,从而实现最少测试获得最高覆盖率。 展开更多
关键词 对象交互 交互测试 抽样oatS
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基于LH-OAT的SWMM模型参数敏感性分析 被引量:21
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作者 朱嘉祺 徐向阳 何爽 《中国农村水利水电》 北大核心 2014年第3期84-87,共4页
为了消除SWMM模型计算过程中的不确定性,明确参数对径流模拟的影响,进行了参数敏感性分析。通过LH-OAT敏感性分析方法,辨析出不同参数在昭通市应用时对径流模拟结果的影响程度,确定对其影响较大的若干敏感参数,为参数的率定提供参考。... 为了消除SWMM模型计算过程中的不确定性,明确参数对径流模拟的影响,进行了参数敏感性分析。通过LH-OAT敏感性分析方法,辨析出不同参数在昭通市应用时对径流模拟结果的影响程度,确定对其影响较大的若干敏感参数,为参数的率定提供参考。研究结果表明对径流系数影响最大的参数是入渗衰减系数,对洪峰流量影响最大的参数是不渗透性。提高了SWMM模型在金沙江流域应用的可靠性。 展开更多
关键词 径流模拟 SWMM模型 敏感性分析 LH-oat
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OAT在非小细胞肺癌中的表达及生物信息学分析 被引量:3
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作者 周丹菲 程西安 +4 位作者 杨拴盈 明宗娟 李维 张秋红 张玉萍 《中国肺癌杂志》 CAS 北大核心 2012年第9期521-530,共10页
背景与目的已有的研究表明,鸟氨酸氨基转移酶(ornithine aminotransferase,OAT)可能参与多种恶性肿瘤的发生和发展,本研究旨在检测非小细胞肺癌(non-small cell lungcancer,NSCLC)中OAT mRNA和蛋白质的表达,并对其进行生物信息学分析。... 背景与目的已有的研究表明,鸟氨酸氨基转移酶(ornithine aminotransferase,OAT)可能参与多种恶性肿瘤的发生和发展,本研究旨在检测非小细胞肺癌(non-small cell lungcancer,NSCLC)中OAT mRNA和蛋白质的表达,并对其进行生物信息学分析。方法通过RT-PCR的方法比较A549和16HBE细胞间OAT mRNA水平的差异;采用免疫组织化学SP法检测55例肺癌组织和17例癌旁肺组织中OAT蛋白表达;利用生物信息学的方法对OAT蛋白的生物信息学特征和相互作用蛋白进行分析,并对筛选出来的相互作用蛋白进行GO注释和信号通路分析。结果①A549中OAT mRNA相对含量较16HBE中低,两者差异约2.85倍。②NSCLC中OAT蛋白的表达明显高于癌旁肺组织(P<0.05);OAT蛋白在鳞癌和腺癌间的表达差异有统计学意义,而与患者性别、年龄、有无淋巴结转移、肿瘤直径及TNM分期无关。③生物信息学分析提示OAT蛋白定位于线粒体、为高度保守的亲水性蛋白,具有Aminotran-3结构域,可能存在多个丝/苏氨酸磷酸化位点,在信号转导、转录和分子转运等方面发挥一定的作用;在筛选出来的54种可能和OAT存在相互作用的蛋白质中,TNF和TRAF6这两种蛋白参与了NF-κB信号通路。结论 OAT可能在NSCLC的发生和发展中发挥重要作用,有望成为肺癌的早期诊断标志物和治疗的新靶点。 展开更多
关键词 鸟氨酸氨基转移酶 肺肿瘤 表达 生物信息学
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基于LH-OAT分布式水文模型参数敏感性分析 被引量:32
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作者 徐会军 陈洋波 +1 位作者 李昼阳 何锦翔 《人民长江》 北大核心 2012年第7期19-23,共5页
为了有效进行分布式水文模型参数的优选,消除模型计算过程中的不确定性,更好地理解参数对水文模拟的影响,开展了模型参数敏感性分析。使用LH-OAT方法,对比分析了3个不同的流域中多个目标函数下的分布式物理水文模型——流溪河模型的参... 为了有效进行分布式水文模型参数的优选,消除模型计算过程中的不确定性,更好地理解参数对水文模拟的影响,开展了模型参数敏感性分析。使用LH-OAT方法,对比分析了3个不同的流域中多个目标函数下的分布式物理水文模型——流溪河模型的参数敏感性,将其参数敏感性归为:极敏感,敏感,一般敏感和不敏感4类。研究表明,模型参数的敏感性并不是一成不变的,在不同流域,不同评价目标下,会发生一定程度的改变。 展开更多
关键词 分布式物理水文模型 LH-oat 参数敏感性 流溪河模型
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基于OAT-GIS技术的地下水污染风险评估指标权重敏感性分析 被引量:6
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作者 梁欢欢 安达 +3 位作者 王月 张伯强 席北斗 吴明红 《环境工程技术学报》 CAS 2016年第2期139-146,共8页
权重赋值准确性是地下水污染风险评估结论可靠性的重要基础,分析并量化指标权重敏感性是验证权重赋值准确性的难点。以我国37个危险废物填埋场为研究对象,基于构建的填埋场地下水污染风险排序指标体系,利用多准则决策分析模型(MCDA)中... 权重赋值准确性是地下水污染风险评估结论可靠性的重要基础,分析并量化指标权重敏感性是验证权重赋值准确性的难点。以我国37个危险废物填埋场为研究对象,基于构建的填埋场地下水污染风险排序指标体系,利用多准则决策分析模型(MCDA)中的层次分析(AHP)模块对各指标进行权重赋值;利用计算所得的权重最小变化量,基于OAT(one-factor-at-atime)方法,应用MCDA的权重敏感性分析模块对14项指标权重进行依次改变,得出14项指标的权重敏感性大小;分析指标权重赋值过程中的不确定性,并基于地理信息系统(GIS)平台,开展可视化的空间数据统计与分析。结果表明:我国37个危险废物填埋场地下水污染风险评估的14项指标中,使用年限、渗滤液量和地形坡度3项指标权重改变后,填埋场地下水污染风险排序变化最大,即这3项指标权重敏感性最高;而包气带渗透性系数指标权重改变导致的风险排序变化最小,说明其权重敏感性最低。该方法可以有效地分析、验证指标权重赋值的准确性,识别权重赋值的不确定性,帮助相关决策者有针对性地进行风险管理。 展开更多
关键词 单因素轮换法 地理信息系统 地下水 权重敏感性分析
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基于LH-OAT的双曲正切模型参数敏感性分析 被引量:1
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作者 刘慧如 吴建华 +1 位作者 孙毅 张玉胜 《水电能源科学》 北大核心 2016年第9期84-86,共3页
为定性了解无资料地区的产流模拟计算时,不同降雨频率下吸收率S_r、导水率K_s和流域持水度B_0三个参数对双曲正切产流模型的影响,利用基于拉丁超立方抽样法和随机OAT法(LH-OAT)的全局敏感性分析方法分析双曲正切产流模型的参数敏感性。... 为定性了解无资料地区的产流模拟计算时,不同降雨频率下吸收率S_r、导水率K_s和流域持水度B_0三个参数对双曲正切产流模型的影响,利用基于拉丁超立方抽样法和随机OAT法(LH-OAT)的全局敏感性分析方法分析双曲正切产流模型的参数敏感性。吕梁兴县廿里铺村的集水小流域产流计算结果表明,模型参数敏感性排序为B_0>S_r>K_s,B_0、S_r随降雨频率的减小呈减弱趋势,K_s随降雨频率的减小呈增强趋势,三个参数随降雨频率变化的速率排序为|vB_0|>|vS_r|>|vK_s|。研究成果为无资料地区的小流域产流计算提供了参考。 展开更多
关键词 LH-oat模型 双曲正切模型 降雨频率 产流计算 敏感性分析
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尿毒素硫酸吲哚酚通过OAT-3诱发HK-2细胞纤维化的作用 被引量:1
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作者 阿孜古力·克热木 艾克拜尔·吾曼尔 +3 位作者 麦伍拉尼·马木提 买买提·依斯热依力 王晨宇 李九智 《西部医学》 2023年第12期1723-1728,共6页
目的探讨尿毒素硫酸吲哚酚(IS)通过有机阴离子转运蛋白-3(OAT-3)诱发人正常肾小管上皮(HK-2)细胞氧化应激和纤维化因子的作用。方法HK-2细胞进行传代培养,待孔板中细胞密度达到80%~90%时分为空白对照组(Control组,加入培养液中正常培养... 目的探讨尿毒素硫酸吲哚酚(IS)通过有机阴离子转运蛋白-3(OAT-3)诱发人正常肾小管上皮(HK-2)细胞氧化应激和纤维化因子的作用。方法HK-2细胞进行传代培养,待孔板中细胞密度达到80%~90%时分为空白对照组(Control组,加入培养液中正常培养不予干预)、IS处理组(IS组,加入250μmoL IS培养),两组细胞培养24 h;同时,进一步在HK-2细胞中用小干扰RNA(siRNA)沉默OAT-3的表达后提取总RNA,其浓度测定并进行RT-PCR以及Western blot试验检测氧化应激(Nox-4)及纤维化因子(Collagen I、TGF-β1、Smad-3、α-SMA)的mRNA和蛋白表达水平;最后HK-2细胞以抗氧化剂N-乙酰半胱氨酸(NAC)预处理后,加入250μmoL IS刺激24 h,采用RT-PCR及Western blot试验检测上述指标的mRNA和蛋白表达水平。结果RT-PCR结果显示,HK-2细胞以250μmoL IS刺激24 h后,IS显著增加HK-2细胞中氧化应激(Nox-4)及纤维化因子(Collagen I、TGF-β1、Smad-3、α-SMA)的mRNA表达水平(P<0.05);HK-2细胞中siRNA沉默OAT-3的表达后发现,IS诱导的上述氧化应激和纤维化因子的mRNA和蛋白表达水平显著降低(P<0.05)。以NAC预处理后,HK-2细胞IS刺激24 h发现,NAC有效抑制IS诱导Nox-4、Collagen I、TGF-β1、Smad-3、α-SMA的mRNA和蛋白表达水平(P<0.05)。结论硫酸吲哚酚经OAT-3摄取到HK-2细胞内诱发氧化应激和纤维化因子高表达。 展开更多
关键词 硫酸吲哚酚 HK-2细胞 oat-3 氧化应激 纤维化因子
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Optimization of the Fermentation Conditions of Sweet Oats by Response Surface Methodology
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作者 张泓 黄艳杰 +1 位作者 黄志兵 许杨 《Agricultural Science & Technology》 CAS 2014年第3期483-487,498,共6页
Objective] The purpose of this study was to evaluate the effects of five variables (steaming time, moisture content before inoculation, inoculation amount, fer-mentation temperature, fermentation time) on the sweetn... Objective] The purpose of this study was to evaluate the effects of five variables (steaming time, moisture content before inoculation, inoculation amount, fer-mentation temperature, fermentation time) on the sweetness of sweet oats, obtained the best fermentation conditions. [Method] Plackett-Burman (PB) was to evaluate the effects of five variables, and selected significant factors. The steepest ascent was used to approach the optimal response surface experimental area. The optimal fer-mentation condition was obtained by central composite design and response surface analysis. [Results] It was indicated that moisture content, inoculation amount and fer-mentation temperature had significant influences on the content of the reducing sugars. The optimal conditions of moisture content, inoculation amount and temper-ature were 45.26%, 0.014%(g/g) and 28 ℃, respectively. The predicted value of the reducing sugar content was 13.16 mg/g. [Conclusion] Under the optimal conditions, the content of the reducing sugars in the sweet oats could be up to 12.91 mg/g, which was on the whole consistent with the predictive maximum value. 展开更多
关键词 Sweet oats FERMENTATION Reducing sugars Response surface methodology
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基于LH-OAT的推理公式法参数敏感性分析 被引量:5
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作者 孙毅 《水力发电》 北大核心 2017年第9期23-25,30,共4页
为定性了解不同洪水频率下汇流参数m,平均比降J两个参数对推理公式法进行无资料地区产流模拟的影响,利用LH-OAT全局敏感度分析方法对推理公式法进行参数敏感性分析。以两个不同分区的小流域(山西省方山县圪洞沟和孝义市下堡河流域)汇流... 为定性了解不同洪水频率下汇流参数m,平均比降J两个参数对推理公式法进行无资料地区产流模拟的影响,利用LH-OAT全局敏感度分析方法对推理公式法进行参数敏感性分析。以两个不同分区的小流域(山西省方山县圪洞沟和孝义市下堡河流域)汇流为例,研究确定不同洪水频率下推理公式法参数的敏感性,确定参数敏感程度和变化规律,为模型参数率定提供数值参考。研究表明,推理公式法参数对洪峰值敏感性排序为m>J;m和J的敏感性随着洪水频率的减小呈现减弱趋势,两个参数随洪水频率变化的速率为V_m>V_J。 展开更多
关键词 敏感性分析 洪水频率 推理公式法 LH-oat模型
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