Aim To study the activity and mechanism of inhibition of myocardial hypertrophy of total flavonoids of Cydonia oblonga Mill. in spontaneously hypertensive rats. Methods Total flavonoids of COM （COMF） were sepa- rate...Aim To study the activity and mechanism of inhibition of myocardial hypertrophy of total flavonoids of Cydonia oblonga Mill. in spontaneously hypertensive rats. Methods Total flavonoids of COM （COMF） were sepa- rated and purified by the optimal process. SHtl were divided into 6 groups： SHR control group （SHR） , captopril group （SHR ＋ CAP, 25 μg · g^-1）, Eucommia ulmoides Oliver group （SHR ＋ EUO, 30 μg · g^-1）, low （SHR ＋ COMF-L, 40 μg · g^-1） ,middle （SHR ＋ COMF-M, 80 μg· g^-1） and high dose （SHR ＋ COMF-H, 160 μg · g^-1） of COMF groups. Wistar-Kyoto （WKY） rats （n= 8 ） were given distilled water as control The drugs were given by intragastric administration for 16 weeks. The histological and pathological examination of the heart were performed and organic damage were valued. The levels of Ang II and ALD in blood and heart were evaluated. The mRNA and protein expression of ACE, ACE2 and AT~ was determined by RT-PCR and Western blot to evaluate the effect of COMF on RAAS. Results Compared with SHR control group, HW, HW/BW, LVM and LVM/BW de- creased in SHR ＋ COMF-M and SHR ＋ COMF-H groups; Cadiomyocyte hypertrophy was inhibited in COMF groups; The concentration of Ang 11 and ALD in heart and blood decreased; ACE and AT1 mRNA and protein ex- pression in heart tissue decreased while ACE2 mRNA expression increased （P 〈 0.01 or P 〈 0.05） , Conclusion Total flavonoids of Cydonia oblonga Mill. showed the effect of inhibition of myocardial hypertrophy in spontaneously hypertensive rats and the mechanism was related to. inhibit the activity of Renin-angiotensin-aldosterone system.展开更多
Aim To study the anti-hypertensive effect of total flavonoids of Cydonia oblonga Mill. in spontaneously hypertensive rats, then preliminarily investigate the mechanism of action based on anti-inflammatory function. Me...Aim To study the anti-hypertensive effect of total flavonoids of Cydonia oblonga Mill. in spontaneously hypertensive rats, then preliminarily investigate the mechanism of action based on anti-inflammatory function. Methods Total flavonoids of COM （COMF） were separated and purified by the optimal process, SHR were divid- ed into 6 groups as SHR control group （SHR）, captopril group （CAP, 25mg· kg^-1 · d^-1）, Eucommia ulmoides Oliver group （EUO, 30 mg · kg^-1· d^-1）, low （COMF-L, 40 mg · kg^-1· d^-1）, middle （COMF-M, 80 mg · kg^-1 · d^-1） and high dose （COMF-H, 160 mg · kg^-1· d ^-1） of COMF groups. 8 Wistar-Kyoto （WKY） rats were given distilled water instead as control. The drugs were given by intragastric administration for 16 weeks, then the levels of IL-1β, IL-6, IL-10, TNF-α and CRP in serum were tested. Results Compared with WKY group, the levels of IL-I~, IL-6, TNF-α and CRP were increased and IL-10 was decreased; Compared with SHR group, the levels of IL-1β, IL-6, TNF-α and CRP were decreased in COMF groups （P 〈0.01 or P 〈0.05） , while IL-10 was increased a little but without any significant difference （P 〈 0.05 ）. Conclusion Total flavonoids of Cydonia ob- longa Mill. showed the anti-hypertensive activity in spontaneously hypertensive rats and the mechanism was related to the function of anti-inflammatory.展开更多
Salacia oblonga (Wall.) belonging to the family Celastraceae is an important medicinal plant available in India and Sri Lanka. It possesses an array of pharmacological and therapeutic properties. In the present study ...Salacia oblonga (Wall.) belonging to the family Celastraceae is an important medicinal plant available in India and Sri Lanka. It possesses an array of pharmacological and therapeutic properties. In the present study phenolic compounds of S. oblonga methanolic extracts were estimated by high performance liquid chromatography (HPLC) indicating the presence of catechin, quercetin, synapic acid and syringic acid. The study involved evaluation of S. oblonga extracts for cytotoxic and cytoprotective effects on human lymphocytes. Aerial extracts have significant protective activity against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) induced cytotoxic activity in comparison to the root extracts. The results relate the cytoprotective effect of S. oblonga due to the presence of poly phenols and other phytochemicals, as they have significant ability to remove free radicals or reactive species and might induce the expression of antioxidant enzymes inside the cells.展开更多
【目的】研究榅桲果实木质化与CAD基因的关系。【方法】基于GenBank报道的近缘物种CAD基因cDNA序列,应用Primer Premier 5.0软件设计PCR扩增引物。提取榅桲总RNA,经反转录后合成cDNA,应用RT-PCR方法成功扩增出CAD基因片段并克隆到pMD18-...【目的】研究榅桲果实木质化与CAD基因的关系。【方法】基于GenBank报道的近缘物种CAD基因cDNA序列,应用Primer Premier 5.0软件设计PCR扩增引物。提取榅桲总RNA,经反转录后合成cDNA,应用RT-PCR方法成功扩增出CAD基因片段并克隆到pMD18-T载体。通过DNAstar软件进行同源序列比对,ClustalX结合MEGA4.1软件构建系统进化树,采用Protparam在线程序分析蛋白质的理化性质,用DNAstar的Protean程序预测二级结构。通过间苯二酚染色鉴定果肉发育时期木质素的积累程度,并利用RT-qPCR对CAD基因在发育期时期的表达规律进行检测。【结果】榅桲CAD基因其开放阅读框(ORF)序列为1 071 bp,编码356个氨基酸,与其他物种序列同源性最高达96%,进化关系上与苹果较近。在果实6个发育时期,木质素积累逐渐降低,而CAD基因表达与果实木质化程度紧密相关,随着木质素合成趋缓呈现一个逐步下调的趋势。【结论】CAD基因是调控榅桲果实木质化的关键基因。展开更多
文摘Aim To study the activity and mechanism of inhibition of myocardial hypertrophy of total flavonoids of Cydonia oblonga Mill. in spontaneously hypertensive rats. Methods Total flavonoids of COM （COMF） were sepa- rated and purified by the optimal process. SHtl were divided into 6 groups： SHR control group （SHR） , captopril group （SHR ＋ CAP, 25 μg · g^-1）, Eucommia ulmoides Oliver group （SHR ＋ EUO, 30 μg · g^-1）, low （SHR ＋ COMF-L, 40 μg · g^-1） ,middle （SHR ＋ COMF-M, 80 μg· g^-1） and high dose （SHR ＋ COMF-H, 160 μg · g^-1） of COMF groups. Wistar-Kyoto （WKY） rats （n= 8 ） were given distilled water as control The drugs were given by intragastric administration for 16 weeks. The histological and pathological examination of the heart were performed and organic damage were valued. The levels of Ang II and ALD in blood and heart were evaluated. The mRNA and protein expression of ACE, ACE2 and AT~ was determined by RT-PCR and Western blot to evaluate the effect of COMF on RAAS. Results Compared with SHR control group, HW, HW/BW, LVM and LVM/BW de- creased in SHR ＋ COMF-M and SHR ＋ COMF-H groups; Cadiomyocyte hypertrophy was inhibited in COMF groups; The concentration of Ang 11 and ALD in heart and blood decreased; ACE and AT1 mRNA and protein ex- pression in heart tissue decreased while ACE2 mRNA expression increased （P 〈 0.01 or P 〈 0.05） , Conclusion Total flavonoids of Cydonia oblonga Mill. showed the effect of inhibition of myocardial hypertrophy in spontaneously hypertensive rats and the mechanism was related to. inhibit the activity of Renin-angiotensin-aldosterone system.
文摘Aim To study the anti-hypertensive effect of total flavonoids of Cydonia oblonga Mill. in spontaneously hypertensive rats, then preliminarily investigate the mechanism of action based on anti-inflammatory function. Methods Total flavonoids of COM （COMF） were separated and purified by the optimal process, SHR were divid- ed into 6 groups as SHR control group （SHR）, captopril group （CAP, 25mg· kg^-1 · d^-1）, Eucommia ulmoides Oliver group （EUO, 30 mg · kg^-1· d^-1）, low （COMF-L, 40 mg · kg^-1· d^-1）, middle （COMF-M, 80 mg · kg^-1 · d^-1） and high dose （COMF-H, 160 mg · kg^-1· d ^-1） of COMF groups. 8 Wistar-Kyoto （WKY） rats were given distilled water instead as control. The drugs were given by intragastric administration for 16 weeks, then the levels of IL-1β, IL-6, IL-10, TNF-α and CRP in serum were tested. Results Compared with WKY group, the levels of IL-I~, IL-6, TNF-α and CRP were increased and IL-10 was decreased; Compared with SHR group, the levels of IL-1β, IL-6, TNF-α and CRP were decreased in COMF groups （P 〈0.01 or P 〈0.05） , while IL-10 was increased a little but without any significant difference （P 〈 0.05 ）. Conclusion Total flavonoids of Cydonia ob- longa Mill. showed the anti-hypertensive activity in spontaneously hypertensive rats and the mechanism was related to the function of anti-inflammatory.
文摘Salacia oblonga (Wall.) belonging to the family Celastraceae is an important medicinal plant available in India and Sri Lanka. It possesses an array of pharmacological and therapeutic properties. In the present study phenolic compounds of S. oblonga methanolic extracts were estimated by high performance liquid chromatography (HPLC) indicating the presence of catechin, quercetin, synapic acid and syringic acid. The study involved evaluation of S. oblonga extracts for cytotoxic and cytoprotective effects on human lymphocytes. Aerial extracts have significant protective activity against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) induced cytotoxic activity in comparison to the root extracts. The results relate the cytoprotective effect of S. oblonga due to the presence of poly phenols and other phytochemicals, as they have significant ability to remove free radicals or reactive species and might induce the expression of antioxidant enzymes inside the cells.
文摘【目的】研究榅桲果实木质化与CAD基因的关系。【方法】基于GenBank报道的近缘物种CAD基因cDNA序列,应用Primer Premier 5.0软件设计PCR扩增引物。提取榅桲总RNA,经反转录后合成cDNA,应用RT-PCR方法成功扩增出CAD基因片段并克隆到pMD18-T载体。通过DNAstar软件进行同源序列比对,ClustalX结合MEGA4.1软件构建系统进化树,采用Protparam在线程序分析蛋白质的理化性质,用DNAstar的Protean程序预测二级结构。通过间苯二酚染色鉴定果肉发育时期木质素的积累程度,并利用RT-qPCR对CAD基因在发育期时期的表达规律进行检测。【结果】榅桲CAD基因其开放阅读框(ORF)序列为1 071 bp,编码356个氨基酸,与其他物种序列同源性最高达96%,进化关系上与苹果较近。在果实6个发育时期,木质素积累逐渐降低,而CAD基因表达与果实木质化程度紧密相关,随着木质素合成趋缓呈现一个逐步下调的趋势。【结论】CAD基因是调控榅桲果实木质化的关键基因。
