Lead （Pb） is one of the most common environmental toxicants, exposure to which can cause significant neurotoxicity and an associated decline in brain function. This study investigated the possible neuroprotective ro...Lead （Pb） is one of the most common environmental toxicants, exposure to which can cause significant neurotoxicity and an associated decline in brain function. This study investigated the possible neuroprotective role oflndigofera oblongifolia leaf methanolic extract （IOLME） against lead-induced neurotoxicity. Rats were intraperitoneally injected with lead acetate, with or without IOLME （intragastric administration for 5 days）, and the neuroprotective effect of IOLME was assessed by measuring the lead concentration, redox status （lipid peroxidation, nitric oxide and glutathione）, enzymatic antioxidant activities （superoxide dismutase, catalase, glutathione peroxidase and reductase）, PCR assays of apoptosis markers （Bax and Bcl-2） and histopathology of the brain. The increases in the lipid peroxidation, nitric oxide, and apoptosis, the decreases in the glutathione level and the activity of antioxidant enzymes, and the altered histology of the brain induced by lead acetate were mitigated in the brain of rats pre-treated with IOLME. These findings indicate that IOLME has beneficial effects and it mitigates lead acetate-induced neurotoxicity via its antiox-idant and anti-apoptotic activities.展开更多
Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China.Understanding of genetic diversity plays an imp...Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China.Understanding of genetic diversity plays an important role in C.oblongifolia survival and sustainable development.The wild C.oblongifolia population was artificially divided into four subpopulations according to the microhabitats,and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds.Then,the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms(SNPs)using specific-locus amplified fragment sequencing(SLAF-seq).Based on these SNPs,we aimed to characterize the genetic diversity and population structure of C.oblongifolia and provide an illumination and reference for effective management of such a small endemic population.The level of genetic diversity was low at the species level,and the progeny subpopulations had a relatively higher genetic diversity than the wild subpopulations.This may be attributed to a high gene flow and an excess heterozygosity to reduce the threat of genetic drift-based hazards.Moreover,the progeny subpopulations had the ability to form new clusters and a great contribution to the genetic structure variation of C.oblongifolia.These results will assist with the development of conservation and management strategies,such as properly evacuating competitive trees to provide more chance for pollen and seed flow in situ conservation,and establishing sufficient seedling plantlets under laboratory conditions for reintroduction to enlarge the effective population size.展开更多
为了探明江苏宝华山宝华鹅耳枥[Carpinus oblongifolia(Hu) Hu et W. C. Cheng]种群现状,在其集中分布区域随机设置了6个样方,并对每个样方进行了实地调查;在此基础上,以树高(H)和胸径(DBH)为指标,对该区域宝华鹅耳枥种群的龄级结构进...为了探明江苏宝华山宝华鹅耳枥[Carpinus oblongifolia(Hu) Hu et W. C. Cheng]种群现状,在其集中分布区域随机设置了6个样方,并对每个样方进行了实地调查;在此基础上,以树高(H)和胸径(DBH)为指标,对该区域宝华鹅耳枥种群的龄级结构进行了分析,并对其所在群落的物种组成和结构特征进行了分析。结果表明:供试6个样方内共有宝华鹅耳枥44株,其种群径级结构呈正态分布,其中,Ⅲ级(2.5 cm≤DBH<7.5 cm)和Ⅳ级(7.5 cm≤DBH<15.0 cm)的株数明显高于其余径级,而Ⅰ级(H<33 cm, DBH<2.5 cm)和Ⅱ级(H≥33 cm, DBH<2.5 cm)的株数偏少。供试6个样方共有维管植物38科55属63种,部分样方缺少灌木层植物;乔木层中Ⅲ级及以上的植株有157株,包括宝华鹅耳枥37株、其他种类120株;宝华鹅耳枥的径级结构仅在1个样方中完整,而在其余5个样方中均呈现断级现象。从乔木层植物的重要值看,宝华鹅耳枥的重要值在多数样方中并不是第1位。综上所述,宝华鹅耳枥在群落中不占有优势地位,且其种群的径级结构不完整,尤以低龄级的幼苗和幼树数量较少,种群难以维持稳定发展。因此,建议采取就地保护辅以迁地保护的保护措施,并进一步探明其幼苗和幼树建成的影响因子,以便创造合适条件,增强宝华鹅耳枥种群的天然更新能力。展开更多
基金the Deanship of Scientific Research at King Saud University for funding the work through the research group project,No.PRG-1436-02
文摘Lead （Pb） is one of the most common environmental toxicants, exposure to which can cause significant neurotoxicity and an associated decline in brain function. This study investigated the possible neuroprotective role oflndigofera oblongifolia leaf methanolic extract （IOLME） against lead-induced neurotoxicity. Rats were intraperitoneally injected with lead acetate, with or without IOLME （intragastric administration for 5 days）, and the neuroprotective effect of IOLME was assessed by measuring the lead concentration, redox status （lipid peroxidation, nitric oxide and glutathione）, enzymatic antioxidant activities （superoxide dismutase, catalase, glutathione peroxidase and reductase）, PCR assays of apoptosis markers （Bax and Bcl-2） and histopathology of the brain. The increases in the lipid peroxidation, nitric oxide, and apoptosis, the decreases in the glutathione level and the activity of antioxidant enzymes, and the altered histology of the brain induced by lead acetate were mitigated in the brain of rats pre-treated with IOLME. These findings indicate that IOLME has beneficial effects and it mitigates lead acetate-induced neurotoxicity via its antiox-idant and anti-apoptotic activities.
基金This work was funded by the Full-Cover Conservation Plan of Native Plant Species in China(KFJ-3W-No1-141)the Open Fund of Jiangsu Key Laboratory for Research and Utilization of Plant Resources(JSPKLB201844).
文摘Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China.Understanding of genetic diversity plays an important role in C.oblongifolia survival and sustainable development.The wild C.oblongifolia population was artificially divided into four subpopulations according to the microhabitats,and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds.Then,the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms(SNPs)using specific-locus amplified fragment sequencing(SLAF-seq).Based on these SNPs,we aimed to characterize the genetic diversity and population structure of C.oblongifolia and provide an illumination and reference for effective management of such a small endemic population.The level of genetic diversity was low at the species level,and the progeny subpopulations had a relatively higher genetic diversity than the wild subpopulations.This may be attributed to a high gene flow and an excess heterozygosity to reduce the threat of genetic drift-based hazards.Moreover,the progeny subpopulations had the ability to form new clusters and a great contribution to the genetic structure variation of C.oblongifolia.These results will assist with the development of conservation and management strategies,such as properly evacuating competitive trees to provide more chance for pollen and seed flow in situ conservation,and establishing sufficient seedling plantlets under laboratory conditions for reintroduction to enlarge the effective population size.
基金Supported by Plant Specimen Digitization and Chinese Virtual Herbarium Establishment(2005DKA21401)a project from the Chinese Academy of Sciences(KSCX2-YW-Z-067)the National Natural Science Foundation of China(30600035)
文摘Litsea rotundifolia var.oblongifolia(Nees)Allen作为豺皮樟学名被广泛使用,而Iozoste rotundifolia var. oblongifolia Nees则长期被认为是其基名,经研究发现Litsea rotundifoliavar.oblongifolia(Nees)Allen的基名是Actinodaphne chinensis var. oblongifolia Nees。
文摘为了探明江苏宝华山宝华鹅耳枥[Carpinus oblongifolia(Hu) Hu et W. C. Cheng]种群现状,在其集中分布区域随机设置了6个样方,并对每个样方进行了实地调查;在此基础上,以树高(H)和胸径(DBH)为指标,对该区域宝华鹅耳枥种群的龄级结构进行了分析,并对其所在群落的物种组成和结构特征进行了分析。结果表明:供试6个样方内共有宝华鹅耳枥44株,其种群径级结构呈正态分布,其中,Ⅲ级(2.5 cm≤DBH<7.5 cm)和Ⅳ级(7.5 cm≤DBH<15.0 cm)的株数明显高于其余径级,而Ⅰ级(H<33 cm, DBH<2.5 cm)和Ⅱ级(H≥33 cm, DBH<2.5 cm)的株数偏少。供试6个样方共有维管植物38科55属63种,部分样方缺少灌木层植物;乔木层中Ⅲ级及以上的植株有157株,包括宝华鹅耳枥37株、其他种类120株;宝华鹅耳枥的径级结构仅在1个样方中完整,而在其余5个样方中均呈现断级现象。从乔木层植物的重要值看,宝华鹅耳枥的重要值在多数样方中并不是第1位。综上所述,宝华鹅耳枥在群落中不占有优势地位,且其种群的径级结构不完整,尤以低龄级的幼苗和幼树数量较少,种群难以维持稳定发展。因此,建议采取就地保护辅以迁地保护的保护措施,并进一步探明其幼苗和幼树建成的影响因子,以便创造合适条件,增强宝华鹅耳枥种群的天然更新能力。
