AIM： To investigate the association between ocular demodex folliculorum infestation and ocular surface manifestations in meibomian gland dysfunction（MGD）.METHODS： Eight-six patients with MGD were enrolled. All enr...AIM： To investigate the association between ocular demodex folliculorum infestation and ocular surface manifestations in meibomian gland dysfunction（MGD）.METHODS： Eight-six patients with MGD were enrolled. All enrolled subjects were tested in the following sequence： ocular surface disease index（OSDI）, slit-lamp biomicroscope examination, corneal surface regularity index（SRI） and surface asymmetry index（SAI）, tear fluid collection, fluorescein tear film break-up time（F-BUT）, corneal fluorescein staining（CFS）, Schirmer I test（SIT） and demodex folliculorum counting. Tear matrix metalloproteinase（MMP）-9 activity was assessed using MMP-9 activity assay kit.RESULTS： Among 86 MGD patients, 40 were positive for demodex. The ocular demodex-positive group showed significantly increased scores of OSDI（25.96±13.74 vs 18.07±11.55, P=0.01）, lid margin abnormality（2.38±0.87 vs 1.98±0.91, P=0.04） and CFS（1.28±2.00 vs 0.94±1.36, P=0.01） compared to the ocular demodex-free group. The tear MMP-9 activity was higher in the ocular demodex-positive group（102.9±32.4 ng/mL） than the ocular demodex-free group（46.2±19.2 ng/mL, P=0.03）. There was no significant difference in meibum quality and expressibility, SRI, SAI, F-BUT and SIT between the two groups（P〉0.05 for each）. No significant correlation was noted between the number of demodex and ocular surface parameters in demodexpositive MGD group（P〉0.05 for each）.CONCLUSION： Ocular demodex folliculorum infestation may be associated with ocular discomfort and ocular surface damage in MGD.展开更多
AIM： To determine the prevalence of ocular demodicosis by both microscopic examination and molecular detection among patients at King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok.METHODS： One hun...AIM： To determine the prevalence of ocular demodicosis by both microscopic examination and molecular detection among patients at King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok.METHODS： One hundred individuals were enrolled in the study and were divided into five age groups. The meibomian gland dysfunction （MGD） score and qualities of cylindrical dandruff （CD） were also determined. Demodex mite infestations of eyelash samples were screened by both microscopic examination and semi-nested polymerase chain reaction （PCR）. RESULTS： The prevalence of ocular demodicosis as determined by microscopic examination was 42% [Demodex folliculorum （D. folliculorum） 41% and Demodex brevis （D. brevis） 1%]. Among patients who had ocular Demodex infestation, 69% have CD and had an average MGD score of 4; in patients without demodicosis, 15.5% had CD and had an average MGD score of 4.12. Prevalence of ocular demodicosis as determined by semi-nested PCR was 79% （D. folliculorum 78% and D. brevis 1%）.CONCLUSION： This is the first report on the prevalence of ocular demodicosis in Thailand. Patients with CD also had Demodex mites present. Semi-nested PCR is better than microscopy for Demodex infestation detection. An extensive survey with more representative samples is required to determine the prevalence in the country.展开更多
Ocular rosacea is an important and underdiagnosed chronic inflammatory disorder observed in children. A clinical spectrum ranging from chronic eyelid inflammation, recurrent ocular redness, photophobia and/or hordeola...Ocular rosacea is an important and underdiagnosed chronic inflammatory disorder observed in children. A clinical spectrum ranging from chronic eyelid inflammation, recurrent ocular redness, photophobia and/or hordeola/chalazions and conjunctival/corneal phlyctenules evolving to neovascularization and scarring may occur. Visual impairment and consequent amblyopia are frequent and corneal perforation although rare is the most feared complication. Ocular manifestations usually precede cutaneous lesions. Although few cases of pediatric ocular rosacea(POR) have been reported in the literature, many cases must have been underdiagnosed or misdiagnosed. The delay in diagnosis is greater than one year in the large majority of cases and may lead to serious ocular sequelae. This review aims to highlight the clinical features of POR, its epidemiology, easy diagnosis and effective treatment. We also propose new diagnostic criteria, in which at least three of the five clinical criteria must be present:(1) Chronic or recurrent keratoconjunctivitis and/or red eye and/or photophobia;(2) Chronic or recurrent blepharitis and/or chalazia/hordeola;(3) Eyelid telangiectasia documented by an ophthalmologist;(4) Primary periorificial dermatitis and/or primary features of rosacea; and (5) Positive familial history of cutaneous and/or ocular rosacea.展开更多
目的:探究蠕形螨感染对睑板腺功能障碍(MGD)患者眼表功能的影响作用。方法:选取2018-04/09南方医科大学附属珠江医院眼科门诊MGD患者94例94眼,入组患者检查以下指标:眼表疾病指数(OSDI)、Schirmer I试验(SⅠt)、泪膜破裂时间(BUT)、角...目的:探究蠕形螨感染对睑板腺功能障碍(MGD)患者眼表功能的影响作用。方法:选取2018-04/09南方医科大学附属珠江医院眼科门诊MGD患者94例94眼,入组患者检查以下指标:眼表疾病指数(OSDI)、Schirmer I试验(SⅠt)、泪膜破裂时间(BUT)、角膜荧光染色(FL)、泪河高度、上睑板腺缺失评分、下睑板腺缺失评分、睑板腺缺失总评分、蠕形螨计数,对比蠕形螨检出阳性组和阴性组以上指标有无差异并分析蠕形螨检出阳性组蠕形螨计数与以上指标相关性。结果: MGD患者94眼中54眼蠕形螨阳性,检出阳性率57%,与年龄无明显相关,男性中检出率高于女性。蠕形螨检出阳性组上睑板腺缺失评分及睑板腺缺失总评分高于蠕形螨检出阴性组[2.00(1.00,2.00) vs 1.00(1.00,1.50)分, P =0.02;3.00(2.00,4.00) vs 2.00(1.00,3.00)分, P =0.04],OSDI、S I t、BUT、FL、下睑板腺缺失评分无差异( P >0.05)。蠕形螨检出阳性的MGD患者中蠕形螨计数与上睑板腺缺失分级评分(rs =0.37, P =0.03)、睑板腺缺失总评分(rs =0.44, P =0.01)呈正相关,与其他检查指标无明显相关性。结论:蠕形螨在睑板腺功能障碍患者眼表功能有一定影响作用,与睑板腺缺失呈正相关,蠕形螨感染数量增加,睑板腺缺失加重,进而加重眼部不适症状。展开更多
基金Supported by the National Natural Science Foundation of China(No.81500693)
文摘AIM： To investigate the association between ocular demodex folliculorum infestation and ocular surface manifestations in meibomian gland dysfunction（MGD）.METHODS： Eight-six patients with MGD were enrolled. All enrolled subjects were tested in the following sequence： ocular surface disease index（OSDI）, slit-lamp biomicroscope examination, corneal surface regularity index（SRI） and surface asymmetry index（SAI）, tear fluid collection, fluorescein tear film break-up time（F-BUT）, corneal fluorescein staining（CFS）, Schirmer I test（SIT） and demodex folliculorum counting. Tear matrix metalloproteinase（MMP）-9 activity was assessed using MMP-9 activity assay kit.RESULTS： Among 86 MGD patients, 40 were positive for demodex. The ocular demodex-positive group showed significantly increased scores of OSDI（25.96±13.74 vs 18.07±11.55, P=0.01）, lid margin abnormality（2.38±0.87 vs 1.98±0.91, P=0.04） and CFS（1.28±2.00 vs 0.94±1.36, P=0.01） compared to the ocular demodex-free group. The tear MMP-9 activity was higher in the ocular demodex-positive group（102.9±32.4 ng/mL） than the ocular demodex-free group（46.2±19.2 ng/mL, P=0.03）. There was no significant difference in meibum quality and expressibility, SRI, SAI, F-BUT and SIT between the two groups（P〉0.05 for each）. No significant correlation was noted between the number of demodex and ocular surface parameters in demodexpositive MGD group（P〉0.05 for each）.CONCLUSION： Ocular demodex folliculorum infestation may be associated with ocular discomfort and ocular surface damage in MGD.
基金Supported by National Science and Technology Development Agency(Thailand)for a Research Chair Grant
文摘AIM： To determine the prevalence of ocular demodicosis by both microscopic examination and molecular detection among patients at King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok.METHODS： One hundred individuals were enrolled in the study and were divided into five age groups. The meibomian gland dysfunction （MGD） score and qualities of cylindrical dandruff （CD） were also determined. Demodex mite infestations of eyelash samples were screened by both microscopic examination and semi-nested polymerase chain reaction （PCR）. RESULTS： The prevalence of ocular demodicosis as determined by microscopic examination was 42% [Demodex folliculorum （D. folliculorum） 41% and Demodex brevis （D. brevis） 1%]. Among patients who had ocular Demodex infestation, 69% have CD and had an average MGD score of 4; in patients without demodicosis, 15.5% had CD and had an average MGD score of 4.12. Prevalence of ocular demodicosis as determined by semi-nested PCR was 79% （D. folliculorum 78% and D. brevis 1%）.CONCLUSION： This is the first report on the prevalence of ocular demodicosis in Thailand. Patients with CD also had Demodex mites present. Semi-nested PCR is better than microscopy for Demodex infestation detection. An extensive survey with more representative samples is required to determine the prevalence in the country.
文摘Ocular rosacea is an important and underdiagnosed chronic inflammatory disorder observed in children. A clinical spectrum ranging from chronic eyelid inflammation, recurrent ocular redness, photophobia and/or hordeola/chalazions and conjunctival/corneal phlyctenules evolving to neovascularization and scarring may occur. Visual impairment and consequent amblyopia are frequent and corneal perforation although rare is the most feared complication. Ocular manifestations usually precede cutaneous lesions. Although few cases of pediatric ocular rosacea(POR) have been reported in the literature, many cases must have been underdiagnosed or misdiagnosed. The delay in diagnosis is greater than one year in the large majority of cases and may lead to serious ocular sequelae. This review aims to highlight the clinical features of POR, its epidemiology, easy diagnosis and effective treatment. We also propose new diagnostic criteria, in which at least three of the five clinical criteria must be present:(1) Chronic or recurrent keratoconjunctivitis and/or red eye and/or photophobia;(2) Chronic or recurrent blepharitis and/or chalazia/hordeola;(3) Eyelid telangiectasia documented by an ophthalmologist;(4) Primary periorificial dermatitis and/or primary features of rosacea; and (5) Positive familial history of cutaneous and/or ocular rosacea.
文摘目的:探究蠕形螨感染对睑板腺功能障碍(MGD)患者眼表功能的影响作用。方法:选取2018-04/09南方医科大学附属珠江医院眼科门诊MGD患者94例94眼,入组患者检查以下指标:眼表疾病指数(OSDI)、Schirmer I试验(SⅠt)、泪膜破裂时间(BUT)、角膜荧光染色(FL)、泪河高度、上睑板腺缺失评分、下睑板腺缺失评分、睑板腺缺失总评分、蠕形螨计数,对比蠕形螨检出阳性组和阴性组以上指标有无差异并分析蠕形螨检出阳性组蠕形螨计数与以上指标相关性。结果: MGD患者94眼中54眼蠕形螨阳性,检出阳性率57%,与年龄无明显相关,男性中检出率高于女性。蠕形螨检出阳性组上睑板腺缺失评分及睑板腺缺失总评分高于蠕形螨检出阴性组[2.00(1.00,2.00) vs 1.00(1.00,1.50)分, P =0.02;3.00(2.00,4.00) vs 2.00(1.00,3.00)分, P =0.04],OSDI、S I t、BUT、FL、下睑板腺缺失评分无差异( P >0.05)。蠕形螨检出阳性的MGD患者中蠕形螨计数与上睑板腺缺失分级评分(rs =0.37, P =0.03)、睑板腺缺失总评分(rs =0.44, P =0.01)呈正相关,与其他检查指标无明显相关性。结论:蠕形螨在睑板腺功能障碍患者眼表功能有一定影响作用,与睑板腺缺失呈正相关,蠕形螨感染数量增加,睑板腺缺失加重,进而加重眼部不适症状。
