Simple Culture Techniques for Germinationpromoting Fungi and Armillariella mellea in Artificial Planting of Gastrodia elata

This study aimed to explore simple culture techniques for symbiotic germination-promoting fungi and Armillariella mellea in artificial planting of Gastrodia elata, i.e., how to use a corner of ordinary house as inoculation room and cultivating site, how to use a pressure cooker for cooking food as sterilization tool, and how to use ordinary household heating as heating measures.

Regulations on Planting Techniques of Harmless Feeding Sorghum bicolor(L.) Moench in Hebei

According to many years of experimental summary, regulations on planting technique of harmless feeding Sorghum bicolor （L.） Moenchwere studied from the application scope, basic demands of planting, preparation be fore seeding, demands of seeding, field management, clipping and harvesting, transportation, storage, ledger management and other technical requirements, and specific measures and technical indicators of the technical regulations were analyzed, so as to provide normalized, standardized, industrial and marketization technical support for the planting of harmless feeding S. bicolor （L.） Moench.

Study on Plant Landscaping Techniques in Book of Superfluities

From the perspective of ideological origins,landscaping principles and techniques,the study planned to conclude relevant content on plant landscaping in Book of Superfluities,so as to enrich landscaping methods and design concept of modern garden plants based on full understanding of plant landscaping in ancient gardens.

Simplified heavy metal staining techniques demonstrated with Fast Plant leaf tissue

Fast Plant(Brassica rapa,Cruciferae)leaf tissuefixed in glutaraldehyde-acrolein and post-fixed in os-mium,was examined for response to several easily-prepared heavy metal stains.Lead and uranium,separately and in combination,gave typical resultsacross the spectrum of cell organellets.As a single stainfollowing osmium,bismuth produced images seeminglyequivalent to lead and uranium.Phosphotungstic acidproduced very good membrane delineation but produceda washed-out background image similar to that from leadstaining.Carbohydrate compounds were especiallyresponsive to ruthenium;the cytoplasm and the matrixof all organelles were also stained very well.Theprocedures were no more demanding than traditionalstaining methods and may be easily used in research andteaching.Fast Plant materials are a reliable,quick andeasy source of living material.

Studies on Plant Population and Stand Establishment Techniques for Increasing Productivity of Rice in Dera Ismail Khan, Pakistan

Rice production in Pakistan is constraint by many factors pertaining to prevalent planting techniques. A research on the feasibility of new planting techniques （direct seeding on flat, transplanting on flat, direct seeding on ridges, transplanting on ridges and parachute planting） in transplanted and direct wet-seeded rice was undertaken at Dera Ismail Khan region of Pakistan＇s North West Frontier Province during 2002 and 2003. Among the planting techniques, the best performance for the yield formation and economic evaluation was noted for transplanting on flat during both years. Chinese parachute planting technology also showed very promising results in most of the parameters. Direct seeding on ridges could not excel transplanting on flat and parachute planting during both cropping seasons. The findings concluded the feasibility of parachute planting technology along with traditional rice transplanting on flat over all other planting techniques being practiced in the area.

Stable Oxygen and Deuterium Isotope Techniques to Identify Plant Water Sources

There is still very little information on the sources of water absorbed by oil palm plant. This information is very important for water management system in oil palm plantation. Thus, this study was carried out to determine current water sources absorbed by the oil palm roots using oxygen (δ18O) and deuterium isotopes (δD) techniques. Sketches of oxygen and deuterium isotope were total rainfall, throughfall, runoff, measurement at 5 soil depths (namely: 20 cm, 50 cm, 100 cm, 150 cm, and 200 cm), and oil palm stem. Results of this study showed huge variance in the values of oxygen and deuterium isotope. Based on Least Significant Difference (LSD) test, there was no significant value in the oxygen and deuterium isotope of stem water and others;however, a similar value was obtained at the depths of 0 - 20 cm and 20 - 50 cm with the stem water. This indicated that oil palm absorbed water from 0 - 50 cm depth. This result agreed with the oil palm rooting system, which has verified that the root quarter is the most active root of oil palm.

Application of Off-season Cultivation Techniques in Landscape Engineering of Beijing Garden Expo

It is a key and difficult point in landscape engineering to keep higher survival rate of off-season cultivation.This paper takes widely-planted species such as Ginkgo biloba,Metasequoia glyptostroboides and Cedrus deodara in Beijing Garden Expo for example,summarizes key off-season cultivation techniques in urban landscaping,elaborated key technical links such as seedling selection,cultivation techniques,after-cultivation maintenance,and provided useful references for the off-season cultivation in urban landscaping of north China.

The RAPD techniques used to assess the genetic diversity in Draba dorneri,a critically endangered plant species

A successful management and preservation of the natural populations is depending on accurate assessment of genetic diversity. Knowledge of genetic diversity within a population is important for the conservation of the species. Our aim was to assess the genetic diversity in Draba dorneri Heuff. population (Brassicaceae family)—an endemic plant species of conservative interest using Random Amplified Polymorphic DNA (RAPD) technique. The plant species is strictly protected at national level as well as at international level through “Convention on the Conservation of European Wildlife and Natural Habitats”, Bern, 1979 European Council. In this study, a total of 52 primers were scored initially. A total of 77 repro- ducible bands with an average of 6.41 bands per primer were obtained from the 12 primers selected. A cluster analysis (UPGAMA) was used to generate a dendrogram based on Dice coefficient. We found 67% similarity between the samples from the two analyzed slopes. Comparing with other rare plants species, our data revealed a higher level of genetic diversity in D. dorneri population in Retezat Mountains.

Real-Time PCR Technique and Its Application in Quantification of Plant Nucleic Acid Molecules

Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of initial template copy numbers as PCR products are generated. This technique significantly simplifies and accelerates the process of producing reproducible quantification of nucleic acid molecules. It not only is a sensitive, accurate and rapid quantitative method, but it also provides an easier way to calculate the absolute starting copy number of nucleic acid molecules to be tested. Together with molecular bio-techniques, like microarray, real-time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics. This review introduces the detailed principles and application of the real-time PCR technique, describes a recently developed system for exact quantification of AUX/IAA genes In Arabidopsis, and discusses the problems with the real-time PCR process.

Study on Planting Techniques of Medicinal Plant Anchusa italica Retz

Anchusa italica Retz.,a perennial herb,has the effects of clearing away heat and toxic materials,and killing parasites to relieve itching.It is mainly used for breast abscess,sore swollen poison,scabies and so on,and serves as one of the commonly used medicinal materials in Uygur medicine.A.italica is distributed in Iran,Europe,Afghanistan and Kazakhstan.It is cultivated in China,and Xinjiang mostly imports it from Pakistan.This study belongs to the technical field of traditional Chinese medicine planting.The planting method solves the technical problems of sowing,field management,harvesting and processing of A.italica.

Damage and deterioration mechanism and curing technique of concrete structure in main coal cleaning plants

Concrete structures in main coal cleaning plants have been rebuilt and reinforced in the coal mines of the Shanghai Datun Energy Sources Co. Ltd., the first colliery of the Pingdingshan Coal Co. Ltd. and the Sanhejian mine of the Xuzhou Mining Group Co. Ltd. In these projects, the operating environment and reliability of concrete structures in the main plants of the three companies were investigated and the safety of the structures inspected. Qualitative and quantitative analyses were made on the special natural, technological and mechanical environments around the structures. On the basis of these analyses, we discuss the long-term, combined actions of the harsh natural (corrosive gases, liquids and solids) and mechanical environments on concrete structures and further investigated the damage and deteriorating mechanisms and curing techniques of concrete structures in the main coal cleaning plants. Our study can provide a theoretical basis for ensuring the reliability of concrete structures in main coal cleaning plants.

Research of Protected Cultivation Techniques of Toona sinensis on Mountain Slopes

In order to prolong the supply period and increase the economic value of Toona sinensis,the protected cultivation of T. sinensis was carried out on mountainous slope to achieve stagger-season planting and the year round supply. The results showed that in late October to midNovember,the temperature was moderate( 1-10℃) and suitable for the lifting of seedlings and temporary planting dormancy. The line spacing of rooted seedlings in greenhouse was( 22-24) cm ×( 4-5) cm and planting density was 900 000-1 200 000 seedlings/ha. The line spacing of seedlings was( 14-13) cm ×( 3-4) cm and planting density was 1 950 000-2 100 000 seedlings/ha. T. sinensis began to sprout at 8-10℃ and stretch at 10-12℃. When the temperature was stable at 14℃ or above,T. sinensis started bolting and emerging leaves. The terminal buds sprouted in 40-50 d for the T. sinensis planted in greenhouse with the day temperature of 15-22℃ and night temperature over 10℃; the terminal buds sprouted in about 15 d for those planted in the greenhouse with day temperature of 20-25℃ and night temperature over 10℃. T. sinensis could be picked 3 times when planted in greenhouse,and the yield was about 24 720 kg/ha.

The Agronomic Techniques as Determinants of the Phenolic Content and the Biological Antioxidant Effect of Palm-Tree Kale

The content of phytochemicals in vegetables is strongly affected by genetic, agronomic, and environmental factors, and by transportation and storage conditions, potentially affecting the sensory and putative health-promoting properties. In this study the impact of different agricultural techniques on the phenolics content and antioxidant activity of extracts from a traditional Italian kale landrace, called palm-tree kale (Brassica oleracea L., ssp. acephala DC, var. sabellica L.) was evaluated. Furthermore, the antioxidant effects of the different extracts were assessed in a biological system using primary cultures of neonatal rat cardiomyocytes. The final aim was to evidence whether and how the agronomic practices can affect the antioxidant properties of kale not only in vitro but also in cells. Results herein reported underline the influence of the crop production strategies in establishing the potential health benefits of a vegetable. This research approach could be useful for the selection of production techniques that are able to provide edible vegetables with higher functional activity, and evidences the need to study the food domain as a whole with the nutrition domain, and to integrate all results in order to have an overall “foodomics” vision allowing the improvement of health and well-being.

Technical Regulations for Planting of Forage Millet Rich in Selenium in Hebei Province

According to the summary of many years of experiments, the application scope, basic demands, preparation before seeding, seeding demands, field management, mowing, transportation, storage, ledger management and other technical requirements of technical regulations for planting of forage millet rich in selenium were studied, and specific measures and technical indicators of the technical regulations were analyzed, aiming to provide normalized, standardized, industrial and marketization technical support for the planting of forage millet rich in selenium.

Performance and High-Yield Cultural Techniques of Two-Line Hybrid Rice Xinlingyou998 at Direct Seeding Cultivation

Xinliangyou 998 is a new indica two-line hybrid rice variety using Xin＇er Sx H0998 as parent. The variety was introduced and demonstrated in Yingshan County in 2016, and showed intermediate growth duration, strong tillering ability, firm culm, tolerance to high fertility and resistance to lodging, tolerance to high tempera- ture and wide range of adaptation, etc. The high-yield cultural techniques were con- cluded.

New Standardized Cultivation Techniques for Tea Seed Production

The tea from tea plants,a kind of traditional leaf plant,is deeply loved by people,but the production of tea fruit and its important functional value have been seriously underestimated for a long time.To this end,the oil plant function and comprehensive utilization value of tea oil from tea fruit were introduced,and a set of new standardized cultivation technique of tea plants that could fully exert the potential of tea seed yield was put forward.The technique could increase the tea seed yield per mu from less than 50kg in traditional tea gardens to more than 200 kg,which broke through the production mode of single leaf picking in traditional tea gardens,gave play to the natural reproductive growth advantages of tea plants,achieved"leaf-seed dual harvest",and promoted the improvement of tea industry quality and efficiency,thereby creating a new path to achieve the"leaf-fruit dual use"of tea plants.

Key Artificial Cultivation Technique by the Naxi Nationality for Dioscorea bulbifera:A Traditional Medicinal and Edible Plant

Dioscorea bulbifera is a medicinal and edible plant of the Naxi nationality living in Lijiang area,and its tuber as a vegetable has the functions of clearing away heat and detoxification,and its bulbil has the functions of clearing away heat and resolving phlegm,relieving cough and relieving asthma,dispersing the accumulation of evils and detoxification,etc.Related studies have shown that it mainly contains steroids,diterpene lactones,flavonoids,polyphenols,alkaloids and so on,and it can also fight cancer,inhibit bacteria,relieve pain,resist inflammation and so on.There is also related literature about hepatotoxicity after taking it.The key technique of traditional vegetable cultivation in Lijiang area is systematically summarized in this paper for reference in the future cultivation process.

Experimental Study on Key Techniques for Indoor Cultivation of Sprout Vegetables in Families

This paper introduced the advantages of planting sprout vegetables in family rooms,expounded assembly principle of indoor cultivating device and the key techniques for indoor cultivating sprout vegetables in families.In the experiment,we assembled a set of indoor cultivating device for sprout vegetables,and summarized the indoor cultivating techniques of sprout vegetables in families.The indoor planting device was suitable for family members to cultivate sprout vegetables in the living rooms,kitchen,balcony and other corner space by using their spare time.It could not only provide high-quality vegetables for families,but also beautify the indoor environments of family rooms,and increase the oxygen content in rooms.This set of indoor cultivating device and techniques was suitable for planting various sprout vegetables(e.g.radish seedling,water spinach seedling,pea seedling,pine willow seedling,sunflower seedling and various bean seedlings,etc.).This is a set of high-efficient spatial planting technique in families,and also an effective way to increase organic vegetable supply for family.

The Technology and Application of the Tree Planting Baseplate in Bare Rock Area

The ecological greening technology of the bare rock area of the arbor governance be-longs to the field of ecological environment restoration, selection of suitable arbors for vegetation restoration in bare rock area, vegetation recovery is the primary approach to governance the ecological environment of bare rock area. The reconstruction of bare rock area arbor-shrub-grass ecosystems is a global environmental science problem. Paying attention to Geological conditions with rich rock fissures and abundant groundwater in these fissures, following the idea to reconstruct ecosystem from arbor to shrub and grass and the principle one tree in a baseplate, authors develop a baseplate technique for tree planting in bare rock area. The baseplate includes the parent body, the root, and the cover to prevent evaporation. Especially, there are filled in nutritional soil for the parent body and the root, and the composition of nutritional soil are selected by test in laboratory, while optimal mix ratio of the composition is obtained. Then, application method in field is put forward. The technique can guarantee survival at early stage and growth in the later for tree planting in the baseplate. In particular, the root provides a good channel to guide tree roots into fissure rock and absorb groundwater in rock. Test in field shows that the baseplate technique has strong practicality in vegetation recovery of bare rock area. Test in field shows that the baseplate technique has strong practicality in vegetation recovery of bare rock area in the world.