Camellia oil and olive oil with superior economic value are easily adulterated with other,cheaper oils.It is difficult to identify both oils by traditional methods because of their similar fatty acid profiles.In the p...Camellia oil and olive oil with superior economic value are easily adulterated with other,cheaper oils.It is difficult to identify both oils by traditional methods because of their similar fatty acid profiles.In the present study,a novel method for qualitative and quantitative analysis ofβ-sitosterol using GC/MS and GC/FID was developed to identify camellia oil and olive oil.The method validation ofβ-sitosterol analysis showed good linearity and satisfactory values for recovery,accuracy,precision,and repeatability.The linear regression coefficient(R^(2))of the calibration curve was 0.9985.An acceptable limit of detection(0.36 mg/100 g)and limit of quantification(1.20 mg/100 g)were achieved.The spiked recoveries were 95.0%to 100.3%.The relative standard deviation(RSD)of within-day precision was less than 3.26%,and the RSD of retention times and peak areas for repeatability were within 0.03%and 1.08%,respectively.The contents ofβ-sitosterol in virgin camellia oil and virgin olive oil were in the range of 14.1–30.2 mg/100 g and 94.3–173.2 mg/100 g,respectively,indicating that theβ-sitosterol content in the former is seven times that in the latter,andβ-sitosterol could be a potential marker for the authentication and adulteration detection of both oils.展开更多
基金This research was funded by the“Pioneer”and“Leading Goose”R&D Program of Zhejiang Province(No.2023C02044)Zhejiang Province Key Research and Development Program(No.2021C02032)Zhejiang Provincial Natural Science Foundation(No.LQ20C200013),China.
文摘Camellia oil and olive oil with superior economic value are easily adulterated with other,cheaper oils.It is difficult to identify both oils by traditional methods because of their similar fatty acid profiles.In the present study,a novel method for qualitative and quantitative analysis ofβ-sitosterol using GC/MS and GC/FID was developed to identify camellia oil and olive oil.The method validation ofβ-sitosterol analysis showed good linearity and satisfactory values for recovery,accuracy,precision,and repeatability.The linear regression coefficient(R^(2))of the calibration curve was 0.9985.An acceptable limit of detection(0.36 mg/100 g)and limit of quantification(1.20 mg/100 g)were achieved.The spiked recoveries were 95.0%to 100.3%.The relative standard deviation(RSD)of within-day precision was less than 3.26%,and the RSD of retention times and peak areas for repeatability were within 0.03%and 1.08%,respectively.The contents ofβ-sitosterol in virgin camellia oil and virgin olive oil were in the range of 14.1–30.2 mg/100 g and 94.3–173.2 mg/100 g,respectively,indicating that theβ-sitosterol content in the former is seven times that in the latter,andβ-sitosterol could be a potential marker for the authentication and adulteration detection of both oils.
