The vitamin E, proteins, amino acids, sugar, trace elements and fatty acids in the oil of Hippophae Rhamnoides L. seeds were quantitatively analyzed. Several methods for analyzing vitamin E were compared with eac...The vitamin E, proteins, amino acids, sugar, trace elements and fatty acids in the oil of Hippophae Rhamnoides L. seeds were quantitatively analyzed. Several methods for analyzing vitamin E were compared with each other and the content of gossypol in the oil was first determined.展开更多
A Hippophae rhamnoides Linn oil nanoemulsion was developed, and its physicochemical properties and in vitro transdermal characteristics were investigated. Then its skin irritation was investigated. The optimum Hippoph...A Hippophae rhamnoides Linn oil nanoemulsion was developed, and its physicochemical properties and in vitro transdermal characteristics were investigated. Then its skin irritation was investigated. The optimum Hippophae rhamnoides Linn oil nanoemulsion prescription was determined using Cremophor EL as an emulsifier, Transcutol P as a co-emulsifier, and isopropyl myristate(IPM) as the oil phase(IPM%: Cremophor EL%: Hippophae rhamnoides Linn oil%= 5:16:20, Km = 4:1). The prepared Hippophae rhamnoides Linn oil nanoemulsion was pale yellow and transparent. Globular droplets were observed under a transmission electron microscope(TEM). The average particle size was 52.2±4.8 nm. The loading capacity was 10.68%, and the oil-in-water(O/W) type nanoemulsion was stable after centrifugation. The CLSM results showed that the fluorescence intensity of the OB Hippophae rhamnoides Linn oil nanoemulsion group was stronger than that of the Hippophae rhamnoides Linn oil cream and Hippophae rhamnoides Linn oil groups in each layer of skin. The study also showed that skin surface treated with the Hippophae rhamnoides Linn oil nanoemulsion had strong fluorescence. The skin depth had weak fluorescence, while the areas near the hair follicle and its appendages had the strongest fluorescence. The skin irritation test showed that the prepared Hippophae rhamnoides Linn oil nanoemulsion was applied to the normal and damaged skin of the rabbit, and there was no apparent redness and swelling. These results suggested that the nanoemulsion coated with Hippophae rhamnoides Linn oil was a potential delivery system for skin disorders.展开更多
在沙棘籽油提取过程中,经粉碎干燥后的籽料颗粒是一种开放体系,脂滴以游离形式存在,采用油红O(Oil Red O,ORO)对其进行染色时,常规方法一次性滴加染液的方式以及后续的洗脱步骤均会导致脂滴迁移、过染等问题出现。研究构建了逐级染色法...在沙棘籽油提取过程中,经粉碎干燥后的籽料颗粒是一种开放体系,脂滴以游离形式存在,采用油红O(Oil Red O,ORO)对其进行染色时,常规方法一次性滴加染液的方式以及后续的洗脱步骤均会导致脂滴迁移、过染等问题出现。研究构建了逐级染色法,采用少量多次滴加染液的方式,并优化了染色间隔时间为1.5 min,染色级数和染液浓度可根据样品的实际油含量进行调整,因而能够客观地显影脂滴在籽料颗粒内的分布状态。以此构建的面积占比法可用于精确测定籽料中的油脂含量,其准确度与萃取质量法相当,两者的相关系数为0.998,并具有较高重现性,相对标准偏差约为2.49%。展开更多
文摘The vitamin E, proteins, amino acids, sugar, trace elements and fatty acids in the oil of Hippophae Rhamnoides L. seeds were quantitatively analyzed. Several methods for analyzing vitamin E were compared with each other and the content of gossypol in the oil was first determined.
基金National Natural Science Foundation of China(Grant No.81460539)the excellent postgraduate project in Xinjiang Medical University(Grant No.CXCY096)
文摘A Hippophae rhamnoides Linn oil nanoemulsion was developed, and its physicochemical properties and in vitro transdermal characteristics were investigated. Then its skin irritation was investigated. The optimum Hippophae rhamnoides Linn oil nanoemulsion prescription was determined using Cremophor EL as an emulsifier, Transcutol P as a co-emulsifier, and isopropyl myristate(IPM) as the oil phase(IPM%: Cremophor EL%: Hippophae rhamnoides Linn oil%= 5:16:20, Km = 4:1). The prepared Hippophae rhamnoides Linn oil nanoemulsion was pale yellow and transparent. Globular droplets were observed under a transmission electron microscope(TEM). The average particle size was 52.2±4.8 nm. The loading capacity was 10.68%, and the oil-in-water(O/W) type nanoemulsion was stable after centrifugation. The CLSM results showed that the fluorescence intensity of the OB Hippophae rhamnoides Linn oil nanoemulsion group was stronger than that of the Hippophae rhamnoides Linn oil cream and Hippophae rhamnoides Linn oil groups in each layer of skin. The study also showed that skin surface treated with the Hippophae rhamnoides Linn oil nanoemulsion had strong fluorescence. The skin depth had weak fluorescence, while the areas near the hair follicle and its appendages had the strongest fluorescence. The skin irritation test showed that the prepared Hippophae rhamnoides Linn oil nanoemulsion was applied to the normal and damaged skin of the rabbit, and there was no apparent redness and swelling. These results suggested that the nanoemulsion coated with Hippophae rhamnoides Linn oil was a potential delivery system for skin disorders.
文摘在沙棘籽油提取过程中,经粉碎干燥后的籽料颗粒是一种开放体系,脂滴以游离形式存在,采用油红O(Oil Red O,ORO)对其进行染色时,常规方法一次性滴加染液的方式以及后续的洗脱步骤均会导致脂滴迁移、过染等问题出现。研究构建了逐级染色法,采用少量多次滴加染液的方式,并优化了染色间隔时间为1.5 min,染色级数和染液浓度可根据样品的实际油含量进行调整,因而能够客观地显影脂滴在籽料颗粒内的分布状态。以此构建的面积占比法可用于精确测定籽料中的油脂含量,其准确度与萃取质量法相当,两者的相关系数为0.998,并具有较高重现性,相对标准偏差约为2.49%。
