The aim of this study was to investigate antibacterial activity of Origanum compactum essential oils collected at three phenological stages on Escherichia coli and Bacillus subtilis. The antibacterial activity was eva...The aim of this study was to investigate antibacterial activity of Origanum compactum essential oils collected at three phenological stages on Escherichia coli and Bacillus subtilis. The antibacterial activity was evaluated using the agar-well diffusion assay. The MIC and MBC values were determined using the micro-dilution assay. The investigation of the antibacterial action was carried out by the evaluation of the effect of O. compactum essential oils on the antibacterial kinetic growth, the integrity of cell membrane and permeability of the cell membrane. The anti-quorum sensing activity was tested by the inhibition of the biofilm formation. The findings of this study showed that O. compactum essential oil has potent antibacterial activities against E. coli and B. subtilis. The lowest inhibition value against B. subtilis was obtained with O. compactum essential oil at the post-flowering stage (MIC=MBC=0.0312%(v/v)). The antibacterial mechanisms of O. compactum essential oils are related to the disturbing of the cell membrane integrity and the increasing of the membrane permeability, which leads to the leakage of genetic materials (DNA and RNA). Moreover, O. compactum essential oils inhibited the formation of the biofilms, a phenotype that has been known to be quorum sensing regulated.展开更多
Objective:To evaluate the larvicidal activity of essential oil of Origanum mtijoruna(Lamiaceae)cultivated in Morocco against Culex pipiens(Diptera:Culicidae).Methods:The analysis and the identification of the various ...Objective:To evaluate the larvicidal activity of essential oil of Origanum mtijoruna(Lamiaceae)cultivated in Morocco against Culex pipiens(Diptera:Culicidae).Methods:The analysis and the identification of the various constituents of essential oil were carried out by gas chromatography coupled with mass spectrometry.Biological test was performed according to a standard methodology inspired by the World Health Organization protocol with slight modification.Results:This oil mainly consisted of monoterpene and sesquiterpenes.The majority compounds are 4-terpinene(28.96%),y-terpinene(18.57%),α-terpinene(12.72%) and sabinene(8.02%).The lethal concentrations(LC_(50) and LC_(90)) measured for the essential oil Origanum majorana,were respectively of the order of 258.71 mg/L and 580.49 mg/L.Conclusions:The results could be useful in search for newer,safer,and more effective natural larvicidal agents.展开更多
To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexne...To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F 2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The results showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F 2a. and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.展开更多
In this study are presented the results of studies for oregano in 16 locations (sites) of Albania for the content of the quantity of oils and their fractions.Albaniais the one of the important diversity centers for Or...In this study are presented the results of studies for oregano in 16 locations (sites) of Albania for the content of the quantity of oils and their fractions.Albaniais the one of the important diversity centers for Oregano because of their adaptation to diverse agro ecological conditions as a result of natural selection. The design of the experiment was based on split plot methods. From the total samples collected, were selected 16 mostly widespread samples of natural populations of origano (Origanum vulgare L. sp. vulgare and sp. hirtum). Essential oil isolation from oregano was performed by hydro distillation. Identification of the components was made by comparing mass spectra of components in essential oils with those from Nist, Wiley and Adams mass spectra libraries. The results show that the total oil content varies from 3.45% to 0.1%. The populations of Oregano in the part of southern Albania have a higher amount of etheric oil compared with those of the North part. The higher content of carvacrol in 55.63%, of the samples was determined in sites of the southern part of Albania, while in the other two investigated area central and north part it was 55.63% and 48.49% respectively. The same indicators were realised also for content of linalool and thymol, while North area has sites with high content of caryophyllen-oxide and β-pinen 3. But, in all sites, the oils of thymol and carvacrolit were dominant.展开更多
The experimental results showed that volatile oil of Origanum vulgare L. (VO) significantly inhibited skindelayed type hypersensitivity (DTH) of mice induced by Dinitrochlorobenzene (DNCB) and sheep red blood cell(SRB...The experimental results showed that volatile oil of Origanum vulgare L. (VO) significantly inhibited skindelayed type hypersensitivity (DTH) of mice induced by Dinitrochlorobenzene (DNCB) and sheep red blood cell(SRBC), and also inhibited the production of hemolysin in serum and SRBC hemolysis mediated by spleen cells atthe same dose of 100 mg·kg-1×7 d ip. The results also indicated it could cause thymus and spleen atrophyin mice at the dose. When the dose of VO was reduced to 50 mg·kg-1·d-1×7 d ip, it significantly increasedthe level of hemolysin in the serum of the mice and SRBC hemolysis mediated by spleen cells. VO couldremarkably inhibited DTH in mice induced by DNCB and SRBC at the dose. It suggested that VO could inhibit specificCell-mediated immunity and had different effects on specific humoral immunity with different doses.展开更多
基金the "Centre National pour la Recherche Scientifique et Technique" (CNRST) "Agence Nationale des Plantes Medicinales et Aromatiques" (ANPMA) for their funding supports
文摘The aim of this study was to investigate antibacterial activity of Origanum compactum essential oils collected at three phenological stages on Escherichia coli and Bacillus subtilis. The antibacterial activity was evaluated using the agar-well diffusion assay. The MIC and MBC values were determined using the micro-dilution assay. The investigation of the antibacterial action was carried out by the evaluation of the effect of O. compactum essential oils on the antibacterial kinetic growth, the integrity of cell membrane and permeability of the cell membrane. The anti-quorum sensing activity was tested by the inhibition of the biofilm formation. The findings of this study showed that O. compactum essential oil has potent antibacterial activities against E. coli and B. subtilis. The lowest inhibition value against B. subtilis was obtained with O. compactum essential oil at the post-flowering stage (MIC=MBC=0.0312%(v/v)). The antibacterial mechanisms of O. compactum essential oils are related to the disturbing of the cell membrane integrity and the increasing of the membrane permeability, which leads to the leakage of genetic materials (DNA and RNA). Moreover, O. compactum essential oils inhibited the formation of the biofilms, a phenotype that has been known to be quorum sensing regulated.
文摘Objective:To evaluate the larvicidal activity of essential oil of Origanum mtijoruna(Lamiaceae)cultivated in Morocco against Culex pipiens(Diptera:Culicidae).Methods:The analysis and the identification of the various constituents of essential oil were carried out by gas chromatography coupled with mass spectrometry.Biological test was performed according to a standard methodology inspired by the World Health Organization protocol with slight modification.Results:This oil mainly consisted of monoterpene and sesquiterpenes.The majority compounds are 4-terpinene(28.96%),y-terpinene(18.57%),α-terpinene(12.72%) and sabinene(8.02%).The lethal concentrations(LC_(50) and LC_(90)) measured for the essential oil Origanum majorana,were respectively of the order of 258.71 mg/L and 580.49 mg/L.Conclusions:The results could be useful in search for newer,safer,and more effective natural larvicidal agents.
文摘To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F 2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The results showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F 2a. and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.
文摘In this study are presented the results of studies for oregano in 16 locations (sites) of Albania for the content of the quantity of oils and their fractions.Albaniais the one of the important diversity centers for Oregano because of their adaptation to diverse agro ecological conditions as a result of natural selection. The design of the experiment was based on split plot methods. From the total samples collected, were selected 16 mostly widespread samples of natural populations of origano (Origanum vulgare L. sp. vulgare and sp. hirtum). Essential oil isolation from oregano was performed by hydro distillation. Identification of the components was made by comparing mass spectra of components in essential oils with those from Nist, Wiley and Adams mass spectra libraries. The results show that the total oil content varies from 3.45% to 0.1%. The populations of Oregano in the part of southern Albania have a higher amount of etheric oil compared with those of the North part. The higher content of carvacrol in 55.63%, of the samples was determined in sites of the southern part of Albania, while in the other two investigated area central and north part it was 55.63% and 48.49% respectively. The same indicators were realised also for content of linalool and thymol, while North area has sites with high content of caryophyllen-oxide and β-pinen 3. But, in all sites, the oils of thymol and carvacrolit were dominant.
文摘The experimental results showed that volatile oil of Origanum vulgare L. (VO) significantly inhibited skindelayed type hypersensitivity (DTH) of mice induced by Dinitrochlorobenzene (DNCB) and sheep red blood cell(SRBC), and also inhibited the production of hemolysin in serum and SRBC hemolysis mediated by spleen cells atthe same dose of 100 mg·kg-1×7 d ip. The results also indicated it could cause thymus and spleen atrophyin mice at the dose. When the dose of VO was reduced to 50 mg·kg-1·d-1×7 d ip, it significantly increasedthe level of hemolysin in the serum of the mice and SRBC hemolysis mediated by spleen cells. VO couldremarkably inhibited DTH in mice induced by DNCB and SRBC at the dose. It suggested that VO could inhibit specificCell-mediated immunity and had different effects on specific humoral immunity with different doses.
