Post-translational modification (PTM) increases the functional diversity of proteins by introducing new functional groups to the side chain of amino acid of a protein. Among all amino acid residues, the side chain of ...Post-translational modification (PTM) increases the functional diversity of proteins by introducing new functional groups to the side chain of amino acid of a protein. Among all amino acid residues, the side chain of lysine (K) can undergo many types of PTM, called K-PTM, such as “acetylation”, “crotonylation”, “methylation” and “succinylation” and also responsible for occurring multiple PTM in the same lysine of a protein which leads to the requirement of multi-label PTM site identification. However, most of the existing computational methods have been established to predict various single-label PTM sites and a very few have been developed to solve multi-label issue which needs further improvement. Here, we have developed a computational tool termed mLysPTMpred to predict multi-label lysine PTM sites by 1) incorporating the sequence-coupled information into the general pseudo amino acid composition, 2) balancing the effect of skewed training dataset by Different Error Cost method, and 3) constructing a multi-label predictor using a combination of support vector machine (SVM). This predictor achieved 83.73% accuracy in predicting the multi-label PTM site of K-PTM types. Moreover, all the experimental results along with accuracy outperformed than the existing predictor iPTM-mLys. A user-friendly web server of mLysPTMpred is available at http://research.ru.ac.bd/mLysPTMpred/.展开更多
This study examined the factors associated with financial support in old age from three primary mechanisms-personal savings,family support,and social insurance-to explore the implications for the future development of...This study examined the factors associated with financial support in old age from three primary mechanisms-personal savings,family support,and social insurance-to explore the implications for the future development of China's rural social welfare system.Cross-sectional surveys of 1392 young and middle-aged rural residents were conducted in July and August2012.The results showed that while social insurance was increasingly acceptable,personal savings and family support still had fundamental value.Combining the three mechanisms,the rural old-age welfare system presented nontraditional features.China's new rural endowment insurance is discussed as a means to address the need for financial support among the rural aging population.展开更多
Using the aggregate measurement of support （AMS） approach of the World Trade Organization （WTO）, this study estimates China＇s product-specific and non-product- specific amber box support levels between 2008 and 2...Using the aggregate measurement of support （AMS） approach of the World Trade Organization （WTO）, this study estimates China＇s product-specific and non-product- specific amber box support levels between 2008 and 2013. The result indicates that China＇s product-specific amber box support level has significantly increased but not yet exceeded the tolerable ceiling of de minimis and remains consistent with WTO rules on agricultural domestic support,＂ that there is still policy room for product-specific amber box support but the ceiling of de minimis has already imposed substantial constraint on China＇s grain price support policies; and that despite rapid increases in non-product-specific amber box support levels, future policy space remains significant. The result also suggests that China should make appropriate adjustments to product-specific amber box support policies in accordance with WTO rules on agricultural domestic support and make proper use of non- product-specific amber box support policies.展开更多
文摘Post-translational modification (PTM) increases the functional diversity of proteins by introducing new functional groups to the side chain of amino acid of a protein. Among all amino acid residues, the side chain of lysine (K) can undergo many types of PTM, called K-PTM, such as “acetylation”, “crotonylation”, “methylation” and “succinylation” and also responsible for occurring multiple PTM in the same lysine of a protein which leads to the requirement of multi-label PTM site identification. However, most of the existing computational methods have been established to predict various single-label PTM sites and a very few have been developed to solve multi-label issue which needs further improvement. Here, we have developed a computational tool termed mLysPTMpred to predict multi-label lysine PTM sites by 1) incorporating the sequence-coupled information into the general pseudo amino acid composition, 2) balancing the effect of skewed training dataset by Different Error Cost method, and 3) constructing a multi-label predictor using a combination of support vector machine (SVM). This predictor achieved 83.73% accuracy in predicting the multi-label PTM site of K-PTM types. Moreover, all the experimental results along with accuracy outperformed than the existing predictor iPTM-mLys. A user-friendly web server of mLysPTMpred is available at http://research.ru.ac.bd/mLysPTMpred/.
基金supported by some projects from Chinese central universities'basic scientific research[Grant No.SKZD201206]Humanities and Social Sciences Project from Education Ministry[grant number:13YJC630131]Nanjing Agricultural University Social Science Fund[Grant No.SK2012006]
文摘This study examined the factors associated with financial support in old age from three primary mechanisms-personal savings,family support,and social insurance-to explore the implications for the future development of China's rural social welfare system.Cross-sectional surveys of 1392 young and middle-aged rural residents were conducted in July and August2012.The results showed that while social insurance was increasingly acceptable,personal savings and family support still had fundamental value.Combining the three mechanisms,the rural old-age welfare system presented nontraditional features.China's new rural endowment insurance is discussed as a means to address the need for financial support among the rural aging population.
基金sponsored by the Cultural and Social Sciences Youth Foundation of the Ministry of Education(Grant No.12YJC790296)the National Natural Sciences Foundation Program(Grants No.71273069 and No.71403277)
文摘Using the aggregate measurement of support （AMS） approach of the World Trade Organization （WTO）, this study estimates China＇s product-specific and non-product- specific amber box support levels between 2008 and 2013. The result indicates that China＇s product-specific amber box support level has significantly increased but not yet exceeded the tolerable ceiling of de minimis and remains consistent with WTO rules on agricultural domestic support,＂ that there is still policy room for product-specific amber box support but the ceiling of de minimis has already imposed substantial constraint on China＇s grain price support policies; and that despite rapid increases in non-product-specific amber box support levels, future policy space remains significant. The result also suggests that China should make appropriate adjustments to product-specific amber box support policies in accordance with WTO rules on agricultural domestic support and make proper use of non- product-specific amber box support policies.
