利用植物多酚原花青素(oligomeric proanthocyanidins,OPC)与大豆分离蛋白(soybean protein isolate,SPI)的相互作用,采用葡萄糖酸内酯诱导结合超声波处理制备OPC-SPI凝胶,旨在改善SPI凝胶性能。研究不同浓度OPC(0.1%、0.3%、0.5%)对SP...利用植物多酚原花青素(oligomeric proanthocyanidins,OPC)与大豆分离蛋白(soybean protein isolate,SPI)的相互作用,采用葡萄糖酸内酯诱导结合超声波处理制备OPC-SPI凝胶,旨在改善SPI凝胶性能。研究不同浓度OPC(0.1%、0.3%、0.5%)对SPI凝胶流变特性和抗氧化活性的影响。结果表明,随着OPC浓度的增加,包埋率增加,荷载量减小,最大荷载量达到35.5753μg/mg。随着OPC浓度的增加,OPC-SPI凝胶的弹性模量和黏性模量增加,损耗因子随之降低,对频率的依赖性降低;但对凝胶的黏度无显著差异。OPC-SPI对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基以及羟自由基的清除能力优于SPI。OPC能够有效增强SPI的凝胶黏弹性和抗氧化活性,为进一步开发新型抗氧化复合SPI凝胶提供了理论依据。展开更多
本文建立了以超高效液相色谱串联质谱法(UPLC-MS)测定低聚原花青素(OPCs)的方法。样品中原花青素在含高铁离子的酸性乙醇溶液中经密闭高温反应后生成氰定离子,通过含1%甲酸,28%水甲醇组成的流动相(72∶28,v/v)经Zorbax Eclipse Plus C1...本文建立了以超高效液相色谱串联质谱法(UPLC-MS)测定低聚原花青素(OPCs)的方法。样品中原花青素在含高铁离子的酸性乙醇溶液中经密闭高温反应后生成氰定离子,通过含1%甲酸,28%水甲醇组成的流动相(72∶28,v/v)经Zorbax Eclipse Plus C18反相色谱柱(2.1 mm×50 mm,1.8μm)分离,采用大气压电喷雾离子源(ESI)在选择离子监测(SIM)模式下以质荷比为287(m/z)的氰定正离子进行检测,并以美国药典(USP)低聚原花青素标准品进行定量。结果显示原花青素在0.2×10^(-6)~2×10^(-6) g/mL的浓度范围内呈良好线性关系,相关系数大于0.999,三个不同浓度水平的平均加标回收率在98%~104%之间,相对标准偏差(RSD)小于5%,此方法具有较高的精密度和准确度。该液质联用方法同时对比了不同标准品之间的差异,当采用低聚原花青素标准品时将有助于保证产品中原花青素测定结果的一致性。展开更多
Hepatitis C virus(HCV)is a major cause of viral hepatitis and currently infects approximately 170 million people worldwide.An infection by HCV causes high rates of chronic hepatitis(>75%)and progresses to liver cir...Hepatitis C virus(HCV)is a major cause of viral hepatitis and currently infects approximately 170 million people worldwide.An infection by HCV causes high rates of chronic hepatitis(>75%)and progresses to liver cirrhosis and hepatocellular carcinoma ultimately.HCV can be eliminated by a combination of pegylatedα-interferon and the broad-spectrum antiviral drug ribavirin;however,this treatment is still associated with poor efficacy and tolerability and is often accompanied by serious side-effects.While some novel direct-actingantivirals against HCV have been developed recently,high medical costs limit the access to the therapy in cost-sensitive countries.To search for new natural anti-HCV agents,we screened local agricultural products for their suppressive activities against HCV replication using the HCV replicon cell system in vitro.We found a potent inhibitor of HCV RNA expression in the extracts of blueberry leaves and then identified oligomeric proanthocyanidin as the active ingredient.Further investigations into the action mechanism of oligomeric proanthocyanidin suggested that it is an inhibitor of heterogeneous nuclear ribonucleoproteins(hn RNPs)such as hn RNP A2/B1.In this review,we presented an overview of functional foods and ingredients efficient for HCV infection,the chemical structural characteristics of oligomeric proanthocyanidin,and its action mechanism.展开更多
文摘利用植物多酚原花青素(oligomeric proanthocyanidins,OPC)与大豆分离蛋白(soybean protein isolate,SPI)的相互作用,采用葡萄糖酸内酯诱导结合超声波处理制备OPC-SPI凝胶,旨在改善SPI凝胶性能。研究不同浓度OPC(0.1%、0.3%、0.5%)对SPI凝胶流变特性和抗氧化活性的影响。结果表明,随着OPC浓度的增加,包埋率增加,荷载量减小,最大荷载量达到35.5753μg/mg。随着OPC浓度的增加,OPC-SPI凝胶的弹性模量和黏性模量增加,损耗因子随之降低,对频率的依赖性降低;但对凝胶的黏度无显著差异。OPC-SPI对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基以及羟自由基的清除能力优于SPI。OPC能够有效增强SPI的凝胶黏弹性和抗氧化活性,为进一步开发新型抗氧化复合SPI凝胶提供了理论依据。
基金Supported by The Collaboration of Regional Entities for the Advancement of Technological Excellence from Japan Science and Technology Agency
文摘Hepatitis C virus(HCV)is a major cause of viral hepatitis and currently infects approximately 170 million people worldwide.An infection by HCV causes high rates of chronic hepatitis(>75%)and progresses to liver cirrhosis and hepatocellular carcinoma ultimately.HCV can be eliminated by a combination of pegylatedα-interferon and the broad-spectrum antiviral drug ribavirin;however,this treatment is still associated with poor efficacy and tolerability and is often accompanied by serious side-effects.While some novel direct-actingantivirals against HCV have been developed recently,high medical costs limit the access to the therapy in cost-sensitive countries.To search for new natural anti-HCV agents,we screened local agricultural products for their suppressive activities against HCV replication using the HCV replicon cell system in vitro.We found a potent inhibitor of HCV RNA expression in the extracts of blueberry leaves and then identified oligomeric proanthocyanidin as the active ingredient.Further investigations into the action mechanism of oligomeric proanthocyanidin suggested that it is an inhibitor of heterogeneous nuclear ribonucleoproteins(hn RNPs)such as hn RNP A2/B1.In this review,we presented an overview of functional foods and ingredients efficient for HCV infection,the chemical structural characteristics of oligomeric proanthocyanidin,and its action mechanism.
