期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
A DNA G-quadruplex converts SOD1 into fibrillar aggregates
1
作者 Wenqian Liu Yulin Xu +5 位作者 Xue Li Yan Meng Huiling Wang Chunrong Liu Changlin Liu Li Wang 《Chinese Chemical Letters》 SCIE CAS CSCD 2021年第7期2322-2326,共5页
Nucleic acids with G4 elements play a role in the formation of aggregates involved in intracellular phase transitions.Our previous studies suggest that different forms of DNA could act as an accelerating template in C... Nucleic acids with G4 elements play a role in the formation of aggregates involved in intracellular phase transitions.Our previous studies suggest that different forms of DNA could act as an accelerating template in Cu/Zn superoxide dismutase(SOD1)aggregation.Here,we examined the regulation of fo rmation and cytotoxicity of the SOD1 aggregates by single-stranded 12-merdeoxynucleotide oligomers(dN)_(12)(N=A,T,G,C;ssDNAs)under acidic conditions.The ssDNAs can be divided into two groups based on their roles in SOD1 binding,exposure of hydrophobic clusters in SOD1,accelerated formation,morphology and cytotoxicity of SOD1 aggregates.G-quadruplexes convert SOD1 into fibrillar aggregates as a template,a fact which was observed for the first time in the nucleic acid regulation of protein aggregation.Moreover,the fibrillar or fibril-like SOD1 species with a G-quadruplex provided by(dG)_(12)were less toxic than the amorphous species with(dN)_(12)(N=A,T).This study not only indicates that both morphology and cytotoxicity of protein aggregates can be regulated by the protein-bound DNAs,but also help us understand roles of nucleic aid G-quadruplexes in the formation of aggregates and membrane less organelles involved in intracellular phase transitions. 展开更多
关键词 SOD1 Deoxynucleotide oligomers(dn)_(12) G-QUADRUPLEXES Fibrillar aggregates
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部