Viscoelasticity of repaired sciatic nerve by poly(lactic-co-glycolic acid) tubes

Medical-grade synthetic poly（lactic-co-glycolic acid） polymer can be used as a biomaterial for nerve repair because of its good biocompatibility, biodegradability and adjustable degradation rate. The stress relaxation and creep properties of peripheral nerve can be greatly improved by repair with poly（lactic-co-glycolic acid） tubes. ＂Fen sciatic nerve specimens were harvested from fresh corpses within 24 hours of death, and were prepared into sciatic nerve injury models by creating a 10 mm defect in each specimen. Defects were repaired by anastomosis with nerve autografts and poly（lactic-co-glycolic acid） tubes. Stress relaxation and creep testing showed that at 7 200 seconds the sciatic nerve anastomosed by poly（lactic-co-glycolic acid） tubes exhibited a greater decrease in stress and increase in strain than those anastomosed by nerve autografts. These findings suggest that poly（lactic-co-glycolic acid） exhibits good viscoelasticity to meet the biomechanical require- ments for a biomaterial used to repair sciatic nerve injury.

A novel artificial nerve graft for repairing longdistance sciatic nerve defects:a self-assembling peptide nanofiber scaffold-containing poly (lactic-co-glycolic acid) conduit

In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold （SAPNS）-containing poly（lactic-co-glycolic acid） （PLGA） conduit （SPC） and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury.

Dorsal root ganglion-derived Schwann cells combined with poly(lactic-co-glycolic acid)/chitosan conduits for the repair of sciatic nerve defects in rats

Schwann cells, nerve regeneration promoters in peripheral nerve tissue engineering, can be used to repair both the peripheral and central nervous systems. However, isolation and puriifcation of Schwann cells are complicated by contamination with ifbroblasts. Current reported measures are mainly limited by either high cost or complicated procedures with low cell yields or purity. In this study, we collected dorsal root ganglia from neonatal rats from which we obtained highly puriifed Schwann cells using serum-free melanocyte culture medium. The purity of Schwann cells （〉95%） using our method was higher than that using standard medium containing fetal bovine serum. The obtained Schwann cells were implanted into poly（lactic-co-glycolic acid）/chi-tosan conduits to repair 10-mm sciatic nerve defects in rats. Results showed that axonal diameter and area were signiifcantly increased and motor functions were obviously improved in the rat sciatic nerve tissue. Experimental ifndings suggest that serum-free melanocyte culture medium is conducive to purify Schwann cells and poly（lactic-co-glycolic acid）/chitosan nerve conduits combined with Schwann cells contribute to restore sciatic nerve defects.

Poly(lactic-co-glycolic acid) conduit for repair of injured sciatic nerve A mechanical analysis

Tensile stress and tensile strain directly affect the quality of nerve regeneration after bridging nerve defects by poly（lactic-co-glycolic acid） conduit transplantation and autogenous nerve grafting for sciatic nerve injury. This study collected the sciatic nerve from the gluteus maximus muscle from fresh human cadaver, and established 10-mm-long sciatic nerve injury models by removing the ischium, following which poly（lactic-co-glycolic acid） conduits or autogenous nerve grafts were transplanted. Scanning electron microscopy revealed that the axon and myelin sheath were torn, and the vessels of basilar membrane were obstructed in the poly（lactic-co-glycolic acid） conduit-repaired sciatic nerve following tensile testing. There were no significant differences in tensile tests with autogenous nerve graft-repaired sciatic nerve. Following poly（lactic-co-glycolic acid） conduit transplantation for sciatic nerve repair, tensile test results suggest that maximum tensile load, maximum stress, elastic limit load and elastic limit stress increased compared with autogenous nerve grafts, but elastic limit strain and maximum strain decreased. Moreover, the tendencies of stress-strain curves of sciatic nerves were similar after transplantation of poly（lactic-co-glycolic acid） conduits or autogenous nerve grafts. Results showed that after transplantation in vitro for sciatic nerve injury, poly（lactic-co-glycolic acid） conduits exhibited good intensity, elasticity and plasticity, indicating that poly（lactic-co-glycolic acid） conduits are suitable for sciatic nerve injury repair.

A porous poly(lactic-co-glycolic acid) scaffold induces innervation in a rabbit model of disc degeneration following annular injury

BACKGROUND： A degradable poly（lactic-co-glycolic acid） （PLGA） scaffold has been used to construct a degradable porous scaffold. This template can simulate the in vivo microenvironment and promote tissue formation. OBJECTIVE： To observe the histopathological changes during degeneration and regeneration of the intervertebral disc, and to analyze the effects of a PLGA scaffold on nerve fiber ingrowth into the lesion in vivo. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Orthopaedic Laboratory, Clinic Medical Research Institution, Sir Run Run Shaw Hospital, Zhejiang University, from December 2007 to July 2008. MATERIALS： PLGA （China Textile Academy）; growth-associated protein-43 （Life-span, USA）; and protein gene product 9.5 antibody （AbD, United Kingdom） were used in this study. METHODS： Three consecutive segments of the intervertebral disc of thirty-two healthy adult male New Zealand rabbits were exposed, comprising L3-4, L4-5 and L5-6. Experimental intervertebral disc （L4-5 and L5-6） models were established by two different methods. In the test （trephine ＋ scaffold） group, a 5-mm deep hole was drilled into the annulus fibrosus using a 3-mm diameter trephine, and the PLGA scaffold was implanted into the hole. In the acupuncture group, the remaining experimental intervertebral disc annulus fibrosus was damaged using a 16G needle at a depth of 5 mm. The L3-4 disc served as a control. MAIN OUTCOME MEASURES： Intervertebral disc degeneration was assessed using radiography, magnetic resonance imaging, and histological examination at various time points post-surgery. Nerve fiber ingrowth into the degenerated intervertebral disc was observed using immunohistochemical staining for growth-associated protein-43 and protein gene product 9.5. RESULTS： Compared with the normal controls, the heights of the damaged intervertebral discs were decreased, and T2 signal intensity was decreased in the test and acupuncture groups 2 weeks post-surgery. Intervertebral disc degeneration was faster in the test group than in the acupuncture group. PLGA was coated with newly formed tissue, gradually degraded, and absorbed, and could induce tissue ingrowth deep into the annulus fibrosus. Results of immunohistochemical staining showed that nerve fibers were distributed in newly formed tissue in the test group, and in the superficial layer or surrounding scar tissue in the acupuncture group. CONCLUSION： A porous PLGA scaffold provides an important biological channel to induce nerve fiber ingrowth deep into the degenerated intervertebral disc.

Controlled release of cisplatin and cancer cell apoptosis with cisplatin encapsulated poly(lactic-co-glycolic acid) nanoparticles

The goal of the present study is to utilize cis-diamminedichloroplatinum (cisplatin) loaded polymer nanoparticles (NPs) to give a controlled, extended, and local drug therapy for the treatment of cancer. We have used biodegradable and biocompatible poly(lactic-co-glycolic acid) (PLGA) to prepare the NPs by adjusting the double emulsion technique using poly(vinylalcohol) as a surface active agent. The PLGA NPs were characterized for particle size and shape, controlled release of cisplatin, and degradation. Cisplatin solubility in deionized water was increased up to 4 mg/mL by simply changing the solution parameters. Cisplatin encapsulated NPs were incubated in phosphate buffered saline (PBS) at 37?C to study the release kinetics of cisplatin. Cisplatin was released in a sustained manner with less than 20% release during a 3-day period followed by 50% release during a 21-day period. A degradation study of PLGA NPs demonstrated the loss of spherical shape during a 21-day period. We also examined the cisplatin sensitive A2780 cell apoptosis when cells were incubated with cisplatin encapsulated PLGA NPs. A large number of cell apoptosis occurred as a result of cisplatin release from the PLGA NPs. These results suggest that cisplatin encapsulated PLGA NPs can be used to treat the cancer cells by injecting them into a localized site minimizing the side effects.

Evaluation of in vitro and in vivo immunostimulatory activities of poly(lactic-co-glycolic acid) nanoparticles loaded with soluble and autoclaved Leishmania infantum antigens: A novel vaccine candidate against visceral leishmaniasis

Objective: To prepare and characterize poly lactic-co-glycolic acid(PLGA) nanoparticles loaded with soluble leishmanial antigen or autoclaved leishmanial antigen and explore in vitro and in vivo immunogenicity of antigen encapsulated nanoparticles. Methods: Water/oil/water double emulsion technique was employed to synthesize PLGA nanoparticles, and scanning electron microscopy, Fourier transform infrared spectroscopy and Zeta-potential measurements were used to identify the characteristics of nanoparticles. Cytotoxicity of synthetized nanoparticles on J774 macrophage were investigated by MTT assays. To determine the in vitro immunostimulatory efficacies of nanoparticles, griess reaction and ELISA was used to measure the amounts of NO and cytokines. During the in vivo analysis, Balb/c mice were immunized with vaccine formulations, and protective properties of nanoparticles were measured by Leishman Donovan unit in the liver following the infection. Cytokine levels in spleens of mice were determined by ELISA. Results: MTT assay showed that neither soluble leishmanial antigen nor autoclaved leishmanial antigen encapsulated nanoparticles showed cytotoxicity against J774 macrophage cells. Contrary to free antigens, both autoclaved leishmanial antigen-nanoparticle and soluble leishmanial antigen-nanoparticle formulations led to a 10 and 16-fold increase in NO amounts by macrophages, respectively. Leishman Donovan unit calculations revealed that soluble leishmanial antigen-nanoparticles and autoclaved leishmanial antigen-nanoparticles yielded 52% and 64% protection against visceral leishmaniasis in mouse models. Besides, in vitro and in vivo tests demonstrated that by increasing IFN-γ and IL-12 levels and inhibiting IL-4 and IL-10 secretions, autoclaved leishmanial antigen-nanoparticles and soluble leishmanial antigennanoparticles triggered Th1 immune response. Conclusions: Both autoclaved leishmanial antigen-nanoparticles and soluble leishmanial antigen-nanoparticles formulations provide exceptional in vitro and in vivo immunostimulatory activities. Hence, PLGA-based antigen delivery systems are recommended as potential vaccine candidates against visceral leishmaniasis.

Effects of poly lactic-co-glycolic acid-Nogo A antibody delayed-release microspheres on regeneration of injured spinal cord in rats

BACKGROUND： Nogo A antigen is the major inhibiting factor blocking regeneration of the injured spinal cord. Neutralizing Nogo A antigens using Nogo A antibodies may help promote neurite regeneration and nervous function recovery. For successful regeneration, sustained release of the antibody from a biodegradable material loaded with Nogo A antibodies to the injury site is required. OBJECTIVE： To compare the therapeutic effects of poly lactic-co-glycolic acid （PLGA）-Nogo A antibody delayed-release microspheres and Nogo A antibody alone on spinal regeneration in Sprague-Dawley rats with complete transverse injury to the spinal cord. DESIGN, TIME AND SETTING： A randomized, controlled animal trial was performed at the Pharmacological Laboratory of West China Center of Medical Sciences, Sichuan University, between October 2007 and January 2008. MATERIALS： Goat anti-rat Nogo A monoclonal antibody was purchased from Santa, American; goat anti-rat neurofilament 200 monoclonal antibody was from Zhongshan Goldenbridge, Beijing, China; PLGA-Nogo A antibody delayed-release microspheres were provided by the College of Pharmacy, Sichuan University. METHODS： A total of 36 adult female Sprague Dawley rats were used to establish models of completely transected spinal cord injury, at T10. Animals were randomly divided into three groups （n=12）： model, Nogo A antibody alone, and Nogo A antibody delayed-release microsphere groups. After transverse injury of the spinal cord, 50 μ L normal saline solution, 50 μL normal saline solution containing 50μL g Nogo A antibody, and 50 μL normal saline solution containing 50 μg Nogo A antibody microspheres were administered to the respective groups at the injury site. MAIN OUTCOME MEASURES： The expression of Nogo A and neurofilament 200 in injured spinal cord was tested immunohistochemically, and motor function of rats was assessed by Basso-Beattie-Bresnahan （BBB） locomotor rating scale. RESULTS： Four weeks after injury, expression of Nogo A in microsphere group was significantly less than model and Nogo A antibody alone groups （P 〈 0.05）; while there was no significant difference between model and Nogo A antibody alone groups （P 〉 0.05）. Ten weeks after injury, microsphere group showed a significantly greater expression of neurofilament 200 than model and Nogo A antibody alone groups （P 〈 0.05）; while no significant difference was found between model and Nogo A antibody alone groups （P 〉 0.05）. At postoperative weeks 5 and 6, the score of BBB locomotor rating scale in microsphere group was significantly greater than the model group （P 〈 0.05）, and at postoperative weeks 7 10, the score was much greater than model and Nogo A antibody alone groups （P 〈 0.05）. CONCLUSION： Nogo A antibody delayed-release microspheres decreased Nogo A expression, increased neurofilament 200 expression in the injured spinal cord of rats, and promoted recovery of motor function through sustained drug release over a long-term period.

A Study of Surface Modification of Poly(lactic-co-glycolic) Acid Using Argon Ion Irradiation

The effect of Argon ion irradiation to the surface properties of poly(lactic-co-glycolic) acid (PLGA) was studied. A beam of 170 keV Argon ions was implanted at different fluencies (1 × 1012, 1 × 1013, 1 × 1014, and 1 × 1015 ions/cm2). X-ray photoelectron spectroscopy (XPS) was used to analyze the evolution of the bonding microstructure of PLGA due to irradiation. Surface morphology was monitored using atomic force microscopy (AFM). AFM analysis shows that film roughness increased to maximum at the dose of 1 × 1014 ions/cm2 where the formations of hillocks were also detected. Hydrophilicity of PLGA is important for their applications in biomedicine such as bioscaffolds. Hydrophilicity was monitored using water contact angle measurements for both unmodified and ion-modified PLGA. It was observed that hydrophilicity of PLGA changes with the ion irradiation. This demonstrates that ion irradiation can be an alternative approach to control hydrophilicity of PLGA. PLGA scaffolds modified with ion irradiation could therefore be more suitable for the biomedical applications.

基于Landsat 8 OLI的东圳水库水质参数反演研究

遥感技术是监测内陆水体水质的有效手段,东圳水库是莆田市水源地,为了对水质进行实时监测,了解其空间分布情况,本文基于Landsat 8 OLI遥感影像,结合124个采样点实测获得的Chl-a、浊度、COD浓度分别构建统计回归模型、BP神经网络模型、XGBoost模型,并采用R^(2)、MAE、RMSE进行精度检验。结果表明BP神经网络模型效果优于统计回归模型,R^(2)均大于0.9,但存在过拟合现象;XGBoost模型可以有效防止过拟合,表现出较强的拟合能力和较高的预测精度。

基于Landsat 8 OLI和资源3号立体像对数据的桉树森林蓄积量估测

【目的】探索Landsat8 OLI数据和立体数据在估算桉树森林蓄积量(forest stock volume,FSV)中的潜力,并且准确地估计桉树的FSV。【方法】以3幅Landsat8 OLI图像和资源3号立体数据为遥感数据源,并且结合少量地面调查数据实现了桉树FSV的遥感估计。研究中提取了三类遥感特征用于估计桉树FSV:第一类是包括植被指数和单波段反射率在内的光谱特征;第二类是基于Landsat 8 OLI图像的单波段提取的8种纹理特征;第三类是基于资源3号立体像对数据和开源的数字高程模型(digital elevation model,DEM)提取的冠层高度模型(canopy height model,CHM)。利用Boruta算法对三类遥感特征进行提取,之后建立了随机森林(random forest,RF)、K-最近邻(K-nearest neighbor,KNN)和支持向量机(support vector machine,SVM)3种机器学习模型以及传统的多源线性回归模型(multiple linear regression,MLR),并以决定系数(R2)、均方根误差(root mean square error,RMSE)和相对均方根误差(relative root mean square error,rRMSE)作为评价指标对模型结果进行评估。【结果】基于ZY-3立体像对数据和开源的DEM数据提取的CHM与桉树的FSV具有很强的相关性,Pearson相关系数达到了0.71。仅仅利用基于Landsat 8 OLI图像提取的光谱和纹理特征难以准确2地估计桉树的FSV,估测模型的R为0.29~0.38,rRMSE为35.65%~43.30%,存在严重的数据饱和问题。2当变量集中加入CHM后,模型的估测精度明显提高,R达到了0.64~0.66,rRMSE为25.74%~26.41%。【结论】使用Landsat 8 OLI数据估算桉树FSV时存在严重的数据饱和问题,并且使用空间分辨率为30 m的纹理特征难以有效地改善森林蓄积量的估计精度。利用资源3号立体像对数据和开源的DEM数据可以提取较为准确的CHM,并且所提取的CHM可以解决改善光学数据的饱和问题,从而提高桉树FSV的估计精度。

基于Acolite模型的Landsat8卫星影像大气校正

大气校正是将卫星传感器观测值转换为真实地表反射率的重要手段,也是构建各类卫星遥感产品的前提基础。本研究以临沂市及周边区域为研究区,应用Acolite模型对Landsat8卫星遥感影像开展植被、水体和人造地表等多种地物目标的大气校正,并对校正结果进行分析及精度评价。通过其与FLAASH模型及Landsat8 Collection2表面反射率数据集进行综合对比,可以发现,Acolite及FLAASH都能够在不同程度去除大气影响。相比而言,Acolite模型大气校正效果优于FLAASH模型,其地表双向反射率光谱曲线与Landsat8 Collection2表面反射率数据变化趋势基本一致。总体来看,Acolite模型对各类地物目标的大气校正整体精度较高,且应用该模型的方案具有操作简便,人工干预少等优点,可为各类地表遥感反演产品的构建提供技术支持。

Mapping lineaments using Landsat 8 OLI and SRTM data;a case study of the eastern part of the Ouarzazate Basin,Morocco

The integration of remote sensing and geographic information system(GIS)was employed in this study to delineate the structural lineaments within the eastern section of the Ouarzazate Basin,situated between the southern front of the Central High Atlas and the northern slopes of the Eastern Anti-Atlas(also known as the Saghro Massif).To achieve this objective,Landsat 8 Operational Land Imager(OLI)and Shuttle Radar Topography Mission(SRTM)data were used.Principal Component Analysis(PCA)was computed and a directional filter was applied to the first PCA and the panchromatic band(B8).Additionally,shading was applied to the SRTM data in four directions;N0°,N45°,N90°,N135°.After removing of the non-geological linear structures,the results obtained,using the automatic extraction method,allowed us to produce a synthetic map that included 1251 lineaments with an average length of 1331 m and was dominated by NE-SW,ENE-WSW and E-W directions,respectively.However,the high lineament density is clearly noted in the Anti-Atlas(Saghro Massif)and at the level of the northern part,extending from the Ait Ibrirne to Arg-Ali Oubourk villages.High lineament density can always be found around the major faults affecting this area.The data collected during the field investigations and from geological maps show that the major direction of the faults and structural accidents range mostly between N45°,N70°and N75°.The correlation of remote sensing results with those collected in the field shows a similarity and coincidence with each other.From these results,it is possible to consider the automatic extraction method as a supplementary kind that can serve classical geology by quickly enriching it with additional data.As shown in this work,this method provides more information when applied in arid areas where the fields are well outcropped.

基于OLI数据的石马河水质参数反演研究

根据河流水质高效快速监测需要,以广东石马河水质为研究对象,利用Landsat8的OLI遥感影像进行化学需氧量反演研究。结果表明:Landsat8卫星OLI遥感影像B2波段与B5波段组合(波长的差值)与石马河水体COD浓度相关性较强,据此建立的三次多项式水质参数反演模型精度较高、误差较小,反演结果可以用于快速研判水污染状况。

Electrospun and woven silk fibroin/poly(lactic-coglycolic acid) nerve guidance conduits for repairing peripheral nerve injury

We have designed a novel nerve guidance conduit（NGC） made from silk fibroin and poly（lactic-co-glycolic acid） through electrospinning and weaving（ESP-NGCs）. Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties, including thickness, tensile stiffness, infrared spectroscopy, porosity, and water absorption were determined in vitro. To assess the biological properties, Schwann cells were cultured in ESP-NGC extracts and were assessed by morphological observation, the MTT assay, and immunohistochemistry. In addition, ESP-NGCs were subcutaneously implanted in the backs of rabbits to evaluate their biocompatibility in vivo. The results showed that ESP-NGCs have high porosity, strong hydrophilicity, and strong tensile stiffness. Schwann cells cultured in the ESP-NGC extract fluids showed no significant differences compared to control cells in their morphology or viability. Histological evaluation of the ESP-NGCs implanted in vivo indicated a mild inflammatory reaction and high biocompatibility. Together, these data suggest that these novel ESP-NGCs are biocompatible, and may thus provide a reliable scaffold for peripheral nerve repair in clinical application.

企业数字化与国际化扩张:基于新OLI范式的研究框架

数字化是企业利用数字技术和信息改变商业运营的过程,数字化的发展促进了企业国际化扩张中各种资源和知识信息的获取和快速流动。数字全球化背景下的新国际商务理论——新OLI范式强调数字化可能促进企业开放式资源的获取、商业关系联结的建立以及对海内外各部门的协调整合从而推进和加速企业国际化扩张。基于此理论,以2010—2019年实施国际化扩张的中国制造业上市企业为研究样本,探讨企业数字化对国际化扩张的影响效果和作用渠道。研究发现,企业数字化对国际化扩张程度和国际化扩张速度均有促进作用,该结果在控制内生性以及一系列稳健性检验后同样成立。开放式资源获取、关系联结和协调整合是企业数字化影响国际化扩张的重要路径。异质性分析表明,企业的技术依赖性、研发投入、外部支持、资产情况和组织冗余是影响企业数字化对国际化扩张的促进作用的重要因素。该研究拓宽了数字全球化情境下企业国际化扩张战略研究的新思路,为我国制造业企业借助数字化转型重塑新时代下的国际竞争优势提供重要参考。

基于TM/OLI影像的肥东县土地利用变化及预测分析

以合肥市肥东县2008年Landsat5 TM影像和2013年、2018年Landsat8 OLI影像为数据源,采用最大似然法、马氏距离法、最小距离法、支持向量机法依次进行土地利用分类与信息提取,最终选择平均总体精度高达96.88%、平均kappa系数为0.95的支持向量机法为研究工具。从土地利用面积变化、单一土地利用动态度、土地利用状态指数等方面分析研究区土地利用的动态变化情况。结果表明肥东县土地利用类型主要是耕地和建设用地,且近10年来,耕地面积不断减少,建设用地呈集中性的趋势增加,植被和水域的变化相对不明显。并利用GM(1,1)模型预测2023年该县植被、水域、耕地和建筑用地面积值分别为70.3228 km^(2)、244.8718 km^(2)、924.6914 km^(2)、975.0727 km^(2)。

基于Landsat TM/OLI影像的哈尔滨市植被覆盖度与景观格局动态变化分析

以哈尔滨市为研究对象,依据2004、2010、2015、2020年Landsat TM/OLI遥感影像,利用归一化植被指数和像元二分模型方法提取该区域的植被覆盖度,结合植被覆盖度转移矩阵,分析植被覆盖度的时空动态变化。根据植被覆盖度分级结果,利用Fragstats4.2软件计算斑块面积、斑块个数、蔓延度与香农多样性指数4个景观格局指数,研究哈尔滨市景观格局的时空演变特征。结果表明,哈尔滨市2004—2020年高植被覆盖度和中高植被覆盖度的区域面积累计和分别占总面积的77.81%、72.54%、73.01%、83.31%,整体处于上升趋势。2004—2020年高植被覆盖度区域的景观百分比占据主导地位,其次是中高植被覆盖度区域,且大部分植被覆盖度区域斑块数量先增加后减少。从景观层次上,斑块聚合度降低、多样性略增加。探究哈尔滨市植被覆盖度和景观格局的动态变化,对城市规划设计和景观格局优化具有一定的指导和参考意义。

Preparation of poly(lactide-co-glycolide)microspheres and evaluation of pharmacokinetics and tissue distribution of BDMC-PLGA-MS in rats

The aim of the present study was to develop a novel long-acting Poly(lactic-co-glycolic acid)(PLGA)-based microspheres formulation of Bisdemethoxycurcum(BDMC) by emulsionsolvent evaporation method. Meanwhile, the effects of the volume ratio of the dispersed phase and continuous phase, the concentration of PLGA and PVA, the theoretical drug loading and stirring speed were investigated. The mean diameter of the microspheres was 8.5 μm and the size distribution was narrow. The encapsulation efficiency(EE) and drug loading efficiency(DLE) of BDME loaded PLGA microspheres(BDMC-PLGA-MS) was 94.18% and 8.14%,respectively. In an in vitro study of drug release, it can be concluded that the BDMC-PLGAMS exhibited sustained and long-term release properties for 96 h. Stability studies suggested that the microspheres we prepared had a very good stability. Furthermore, the results of an in vivo study indicated that the BDMC-PLGA-MS had sustained release effect and was mainly distributed in the lung tissue, and less distribution in other tissues, which indicated that microspheres could be an effective parenteral carrier for the delivery of BDMC in lung cancer treatment.

基于Landsat 8 OLI卫星遥感数据的黄前水库水源地保护区水土流失监测

近年来,为了提升饮用水水源地的综合监管水平,遥感监测技术得到了广泛应用。本文中基于landsat8 OLI卫星遥感数据,采用ENVI、Arc GIS等遥感图像处理技术,研究了黄前水库水源地保护区的植被覆盖度及水土流失分布特征。结果表明:黄前水库饮用水水源地保护区的林草覆盖度较高,占保护区总面积的57.32%;而水土流失较为严重,土壤侵蚀面积占55.30%,其中,以轻度、中度为主。该成果为黄前水库饮用水水源地保护区的水土流失监测和生态环境综合治理提供了技术支撑。