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siRNA对胃癌细胞系BGC-823生长抑素基因表达的抑制效应 被引量:2
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作者 朱晓燕 张艳 张钦宪 《解剖学杂志》 CAS CSCD 北大核心 2006年第4期426-429,449,共5页
目的:研究siRNA对胃癌细胞BGC-823生长抑素(SOM)分泌的抑制效应。方法:设计4条针对SOM基因不同位点的寡核苷酸序列,应用RiboMAXT7体外转录合成siRNA并转染胃癌细胞系BGC-823,经RT- PCR、免疫细胞化学法检测SOM mRNA和蛋白的表达水平,... 目的:研究siRNA对胃癌细胞BGC-823生长抑素(SOM)分泌的抑制效应。方法:设计4条针对SOM基因不同位点的寡核苷酸序列,应用RiboMAXT7体外转录合成siRNA并转染胃癌细胞系BGC-823,经RT- PCR、免疫细胞化学法检测SOM mRNA和蛋白的表达水平,筛选抑制效果最佳的序列,MTT法检测BGC-823细胞的增殖变化。结果:转染后24、48及72h,SOM基因的表达被抑制,抑制效率有差异,并呈浓度及时间依赖性。结论:体外合成siRNA抑制胃癌细胞系SOM基因的表达,增强了细胞的增殖能力。 展开更多
关键词 生长抑素 RNA干扰 抑制
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人胃、十二指肠粘膜SS,AVP,EK和β-EP含量测定及其临床意义 被引量:1
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作者 张宏博 张忠兵 +2 位作者 张学庸 胡家露 樊代明 《第四军医大学学报》 1995年第3期208-210,共3页
作者应用放免分析法对24例十二指肠溃疡(DU),15例胃溃疡(GU)患者胃、十二指肠粘膜进行了生长抑素(SS),精氨酸加压素(AVP)、脑啡肽(EK)和β-内啡肽(β-EP)含量测定.同时以20例慢性浅表性胃炎(CS... 作者应用放免分析法对24例十二指肠溃疡(DU),15例胃溃疡(GU)患者胃、十二指肠粘膜进行了生长抑素(SS),精氨酸加压素(AVP)、脑啡肽(EK)和β-内啡肽(β-EP)含量测定.同时以20例慢性浅表性胃炎(CSG)为对照.结果表明,DU患者球、窦和体部粘膜SS含量(依次为153.5±27.5,330.9±23.9和177.1±30.9pg/mg)显著低于(P<0.05)对照(依次为253.7±34.2,588.9±85.3和288.8±40.5pg/mg),EK(依次为98.6±13.2,108.2±16.5和58.5±10.3)和β-EP(依次为113.0±22.6,88.7±16.8和82.8±18.8pg/mg)显著高于(P<0.01)对照(EK依次为50.1±8.8,62.9±11.4和30.9±3.0;β-EP依次为82.3±8.1,39.8±6.8和49.8±9.1).DU患者球部粘膜AVP(52.3±7.0pg/mg)显著高于(P<0.05)CSG(37.7±4.3pg/mg).GU组上述激素含量与对照组比较均无显著差异.提示:①AVP,SS,EK和β-EP在人胃、十二指肠皆有分布;②粘? 展开更多
关键词 胃肠激素 消化性溃疡 生长抑素 精氨酸加压素
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Biochemically curative surgery for gastrinoma in multiple endocrine neoplasia type 1 patients 被引量:5
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作者 Masayuki Imamura Izumi Komoto +5 位作者 Shuichi Ota Takuya Hiratsuka Shinji Kosugi Ryuichiro Doi Masaaki Awane Naoya Inoue 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第10期1343-1353,共11页
AIM: To search for the optimal surgery for gastrinoma and duodenopancreatic neuroendocrine tumors in patients with multiple endocrine neoplasia type 1. METHODS: Sixteen patients with genetically confirmed multiple e... AIM: To search for the optimal surgery for gastrinoma and duodenopancreatic neuroendocrine tumors in patients with multiple endocrine neoplasia type 1. METHODS: Sixteen patients with genetically confirmed multiple endocrine neoplasia type 1 (MEN 1) and Zollinger-Ellison syndrome (ZES) underwent resection of both gastrinomas and duodenopancreatic neuroendocrine tumors (NETs) between 1991 and 2009. For localization of gastrinoma, selective arterial secretagogue injection test (SASI test) with secretin or calcium solution was performed as well as somatostatin receptor scintigraphy (SRS) and other imaging methods such as computed tomography (CT) or magnetic resonance imaging (MRI). The modus of surgery for gastrinoma has been changed over time, searching for the optimal surgery: pancreaticoduodenectomy (PD) was first performed guided by localization with the SAST test, then local resection of duodenal gastrinomas with dissection of regional lymph nodes (LR), and recently pancreas-preserving total duodenectomy (PPTD) has been performed for multiple duodenal gastrinomas. RESULTS: Among various types of preoperative localizing methods for gastrinoma, the SASI test was the most useful method. Imaging methods such as SRS or CT made it essentially impossible to differentiate functioning gastrinoma among various kinds of NETs. However, recent imaging methods including SRS or CT were useful for detecting both distant metastases and ectopic NETs; therefore they are indispensable for staging of NETs. Biochemical cure of gastrinoma was achieved in 14 of 16 patients (87.5%); that is, 100% in 3 patients who underwent PD, 100% in 6 patients who underwent LR (although in 2 patients (33.3%) second LR was performed for recurrence of duodenal gastri- noma), and 71.4% in 7 patients who underwent PPTD. Pancreatic NETs more than 1 cm in diameter were resected either by distal pancreatectomy or enucleations, and no hepatic metastases have developed postoperatively. Pathological study of the resected specimens revealed co-existence of pancreatic gastrinoma with duodenal gastrinoma in 2 of 16 patients (13%), and G cell hyperplasia and/or microgastrinoma in the duodenal Brunner's gland was revealed in all of 7 duodenal specimens after PPTD. CONCLUSION: Aggressive resection surgery based on accurate localization with the SASI test was useful for biochemical cure of gastrinoma in patients with MEN 1.Imamura Metal. Curative resection of gastrinoma in MEN-1 展开更多
关键词 GASTRINOMA Duodenopancreatic neuroendocrine tumors Multiple endocrine neoplasia type 1 Selective arterial secretagogue injection test 5omatostatin receptor scintigraphy Pancreas-preserving total duode- nectomy PANCREATICODUODENECTOMY
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生长抑素——可能是人体内影响最广泛的一个胃肠激素 被引量:23
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作者 黄象谦 《华人消化杂志》 1998年第2期93-96,共4页
生长抑素———可能是人体内影响最广泛的一个胃肠激素黄象谦Subjectheadingssomatostatin/metabolism;somatostatin/physiology;somatostatin/an... 生长抑素———可能是人体内影响最广泛的一个胃肠激素黄象谦Subjectheadingssomatostatin/metabolism;somatostatin/physiology;somatostatin/analogsandderivativ... 展开更多
关键词 生长抑素 胃肠激素 代谢 生理学 奥曲肽
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