Manipulating an exogenous or endogenous gene of interest at a defined level is critical for a wide variety of experiments.The Gal4/UAS system has been widely used to direct gene expression for studying complex genetic...Manipulating an exogenous or endogenous gene of interest at a defined level is critical for a wide variety of experiments.The Gal4/UAS system has been widely used to direct gene expression for studying complex genetic and biological problems in Drosophila melanogaster and other model organisms.Driven by a given tissue-specific Gal4,expressing UAS-transgene or UAS-RNAi(RNA interference)could be used to up-or down-regulate target gene expression,respectively.However,the efficiency of the Gal4/UAS system is roughly predefined by properties of transposon vector constructs and the insertion site in the transgenic stock.Here,we describe a simple way to modulate optomotor blind(omb)expression levels in its endogenous expression region of the wing disc.We co-expressed UAS-omb and UAS-omb-RNAi together under the control of dpp-Gal4 driver which is expressed in the omb expression region of the wing pouch.The repression effect is more sensitive to temperature than that of overexpression.At low temperature,overexpression plays a dominant role but the efficiency is attenuated by UAS-omb-RNAi.In contrast,at high temperature RNAi predominates in gene expression regulation.By this strategy,we could manipulate omb expression levels at a moderate level.It allows us to manipulate omb expression levels in the same tissue between overexpression and repression at different stages by temperature control.展开更多
本研究利用WRF模式及其三维变分同化系统实现了对NOAA-16 AMSU-A微波资料的直接同化,针对2010年6月19日江西地区的一次强降水过程开展模拟与同化试验,并利用中国区域土壤湿度同化系统(CLSMDAS—China Land Soil Moisture Data Assimilat...本研究利用WRF模式及其三维变分同化系统实现了对NOAA-16 AMSU-A微波资料的直接同化,针对2010年6月19日江西地区的一次强降水过程开展模拟与同化试验,并利用中国区域土壤湿度同化系统(CLSMDAS—China Land Soil Moisture Data Assimilation System)输出的土壤湿度值替换NCEP(National Centers for Environmental Prediction)资料中的土壤湿度,研究土壤湿度初值对辐射率资料直接同化中观测场与背景场偏差调整的影响。结果表明:采用CLSMDAS输出土壤湿度初值条件下模拟的亮温值与实际观测值更为接近,经过质量控制和偏差订正后更多的观测资料能够进入到同化系统中,说明改进的土壤湿度初值条件下观测算子的计算值得到正的调整,对低层地表通道的改进效果明显,尤其以50.3 GHz的窗区通道3的结果最为理想;针对此次强降水过程中24 h累积降水分布的模拟结果,CLSMDAS输出土壤湿度初值条件下同化AMSU-A资料,能够较为准确的把握整个雨带的走向、大雨以上级别降水的落区范围、降水中心落区及强度等。说明准确的土壤湿度初值能够改进卫星辐射率资料的同化结果,进而提高数值模式的模拟预报能力。展开更多
基金supported by National Natural Science Foundation of China(NSFC31402021and NSFC31672364)2018 Special Talents Projects in Shanxi Province,China(201805D211019).
文摘Manipulating an exogenous or endogenous gene of interest at a defined level is critical for a wide variety of experiments.The Gal4/UAS system has been widely used to direct gene expression for studying complex genetic and biological problems in Drosophila melanogaster and other model organisms.Driven by a given tissue-specific Gal4,expressing UAS-transgene or UAS-RNAi(RNA interference)could be used to up-or down-regulate target gene expression,respectively.However,the efficiency of the Gal4/UAS system is roughly predefined by properties of transposon vector constructs and the insertion site in the transgenic stock.Here,we describe a simple way to modulate optomotor blind(omb)expression levels in its endogenous expression region of the wing disc.We co-expressed UAS-omb and UAS-omb-RNAi together under the control of dpp-Gal4 driver which is expressed in the omb expression region of the wing pouch.The repression effect is more sensitive to temperature than that of overexpression.At low temperature,overexpression plays a dominant role but the efficiency is attenuated by UAS-omb-RNAi.In contrast,at high temperature RNAi predominates in gene expression regulation.By this strategy,we could manipulate omb expression levels at a moderate level.It allows us to manipulate omb expression levels in the same tissue between overexpression and repression at different stages by temperature control.
文摘本研究利用WRF模式及其三维变分同化系统实现了对NOAA-16 AMSU-A微波资料的直接同化,针对2010年6月19日江西地区的一次强降水过程开展模拟与同化试验,并利用中国区域土壤湿度同化系统(CLSMDAS—China Land Soil Moisture Data Assimilation System)输出的土壤湿度值替换NCEP(National Centers for Environmental Prediction)资料中的土壤湿度,研究土壤湿度初值对辐射率资料直接同化中观测场与背景场偏差调整的影响。结果表明:采用CLSMDAS输出土壤湿度初值条件下模拟的亮温值与实际观测值更为接近,经过质量控制和偏差订正后更多的观测资料能够进入到同化系统中,说明改进的土壤湿度初值条件下观测算子的计算值得到正的调整,对低层地表通道的改进效果明显,尤其以50.3 GHz的窗区通道3的结果最为理想;针对此次强降水过程中24 h累积降水分布的模拟结果,CLSMDAS输出土壤湿度初值条件下同化AMSU-A资料,能够较为准确的把握整个雨带的走向、大雨以上级别降水的落区范围、降水中心落区及强度等。说明准确的土壤湿度初值能够改进卫星辐射率资料的同化结果,进而提高数值模式的模拟预报能力。
