Alzheimer's disease(AD)is affected by genetic factors.Polymorphisms in the glutathione S-transfe rase omega-1(Gsto1)gene have been shown by genetic correlation analyses performed in different ethnic populations to...Alzheimer's disease(AD)is affected by genetic factors.Polymorphisms in the glutathione S-transfe rase omega-1(Gsto1)gene have been shown by genetic correlation analyses performed in different ethnic populations to be genetic risk factors for AD.Gene expression profile data from BXD recombinant inbred mice were used in combination with genetic and bioinformatic analyses to chara cterize the mechanisms underlying regulation of Gstol variation regulation and to identify network membe rs that may contribute to AD risk or progression.Allele-specific assays confirmed that variation in Gstol expression is controlled by cis-expression quantitative trait loci.We found that Gstol mRNA levels were related to several central nervous system traits,such as glial acidic fibrillary protein levels in the caudate putamen,co rtical gray matter volume,and hippocampus mossy fiber pathway volume.We identified 2168 genes whose expression was highly correlated with that of Gsto1.Some genes were enriched for the most common neurodegenerative diseases.Some Gsto1-related genes identified in this study had previously been identified as susceptibility genes for AD,such as APP,Grin2 b,Ide,and Psenen.To evaluate the relationships between Gstol and candidate network members,we transfected astrocytes with Gstol siRNA and assessed the effect on putative downstream effecto rs.We confirmed that knockdown of Gstol had a significant influence on Pa2g4 expression,suggesting that Pa2g4 may be a downstream effector of Gstol,and that both genes intera ct with other genes in a network during AD pathogenesis.展开更多
We investigate the constraints on a generalized Chaplygin gas （GCG） model using the gold sample type-Ia supernovae （She Ia） data, the new Supernova Legacy Survey （SNLS） Sne Ia data and the size of baryonic acous...We investigate the constraints on a generalized Chaplygin gas （GCG） model using the gold sample type-Ia supernovae （She Ia） data, the new Supernova Legacy Survey （SNLS） Sne Ia data and the size of baryonic acoustic oscillation peak found in Sloan Digital Sky Survey （SDSS）. In a spatially flat universe case we obtain, at a 95.4% confidence level, A8 = 0.76^＋0.07 -0.07 and α= 0.028^＋0.322 -0.2382 Our results are consistent with the ACDM model （α= 0）, but rule out the standard Chaplygin gas model （α= 1）.展开更多
Background: The Mediterranean Diet (MD) has been linked to a reduced risk of developing degenerative diseases, including atherosclerosis, heart stroke, diabetes, arthritis and cancer. However, only a few scientific in...Background: The Mediterranean Diet (MD) has been linked to a reduced risk of developing degenerative diseases, including atherosclerosis, heart stroke, diabetes, arthritis and cancer. However, only a few scientific investigations have attempted to validate this impression. The ingredients of the MD include significant amounts of omega (ω3, ω6, and ω9) unsaturated fatty acids (UFAs). A few studies of these UFAs in the prevention or treatment of arthritis have yielded controversial results, but a general belief regarding their beneficial effects has prevailed. Objective: To investigate the effects of three relevant UFAs, namely Docosahexaenoic Acid (DHA), Arachidonic Acid (AA), and Oleic Acid (OA) (ω3, ω6, and ω9, respectively), in the development of arthritis using a murine model of Collagen-Induced Arthritis (CIA). Methods: DBA-1 mice were immunized with chicken collagen type II (CII) and were subsequently treated with ω-UFAs for 53 days. Dexamethasone (DEXA) was used as a positive anti-inflammatory agent. The effect of the treatments was evaluated through several parameters: inflammation indices, antibody levels, cell prolifera- tion, and histopathological findings. Results and Conclusion: The anti-inflammatory effect of the tested substances was inversely correlated with the histopathological findings: a greater anti- inflammatory effect was associated with less articular damage. Oleic acid (ω9) was the most efficient anti-inflammatory UFA, followed by DHA and then AA. DEXA completely inhibited the development of arthritis, whereas the untreated CII-immunized mice developed the most severe articular damage. DBA-1 mice with CII-induced arthritis constitute an adequate model for the study of arthritis and its treatment.展开更多
目的分析低剂量三氯乙烯(TCE)诱导的细胞兴奋效应时,谷胱甘肽转移酶Ω-1(GSTO-1)的表达变化。方法利用前期的双向电泳和质谱结果,参照前期兴奋效应的剂量设计,提取染毒细胞的mRNA和总蛋白,进一步使用实时定量RT-PCR和Western blotting...目的分析低剂量三氯乙烯(TCE)诱导的细胞兴奋效应时,谷胱甘肽转移酶Ω-1(GSTO-1)的表达变化。方法利用前期的双向电泳和质谱结果,参照前期兴奋效应的剂量设计,提取染毒细胞的mRNA和总蛋白,进一步使用实时定量RT-PCR和Western blotting分别从mRNA和蛋白水平上验证GSTO-1的表达变化。结果 Western blotting分析发现,0.1mmol/LTCE剂量组能诱导GSTO-1蛋白表达是对照组的2倍,但随着剂量的增加,蛋白表达改变不明显(与对照组相比,P<0.05)。RT-PCR结果提示:低剂量TCE能诱导GSTO-1基因表达增加,随着剂量的增加,表达反而有所减少。结论低剂量TCE能诱导GSTO-1基因及蛋白表达增加。展开更多
基金the Natural Science Foundation of China,Nos.81200828(to YC),32070998(to GC)the Key Research and Development Program(Social Development)of Jiangsu Province,No.BE2020667(to GC)+1 种基金the Foundation of Jiangsu Province"333 Project High-level Talents",No.BRA2020076(to GC)the Priority Academic Program Development of Jiangsu Higher Education Institutes(PAPD)。
文摘Alzheimer's disease(AD)is affected by genetic factors.Polymorphisms in the glutathione S-transfe rase omega-1(Gsto1)gene have been shown by genetic correlation analyses performed in different ethnic populations to be genetic risk factors for AD.Gene expression profile data from BXD recombinant inbred mice were used in combination with genetic and bioinformatic analyses to chara cterize the mechanisms underlying regulation of Gstol variation regulation and to identify network membe rs that may contribute to AD risk or progression.Allele-specific assays confirmed that variation in Gstol expression is controlled by cis-expression quantitative trait loci.We found that Gstol mRNA levels were related to several central nervous system traits,such as glial acidic fibrillary protein levels in the caudate putamen,co rtical gray matter volume,and hippocampus mossy fiber pathway volume.We identified 2168 genes whose expression was highly correlated with that of Gsto1.Some genes were enriched for the most common neurodegenerative diseases.Some Gsto1-related genes identified in this study had previously been identified as susceptibility genes for AD,such as APP,Grin2 b,Ide,and Psenen.To evaluate the relationships between Gstol and candidate network members,we transfected astrocytes with Gstol siRNA and assessed the effect on putative downstream effecto rs.We confirmed that knockdown of Gstol had a significant influence on Pa2g4 expression,suggesting that Pa2g4 may be a downstream effector of Gstol,and that both genes intera ct with other genes in a network during AD pathogenesis.
基金Supported in part by the National Natural Science Foundation of China under Grants Nos 10375023 and 10575035, the Programme for NCET under Grant No 04-0784, the Key Project of the Ministry of Education of China （No 205110）, and the Doctor Foundation of CSUFT.
文摘We investigate the constraints on a generalized Chaplygin gas （GCG） model using the gold sample type-Ia supernovae （She Ia） data, the new Supernova Legacy Survey （SNLS） Sne Ia data and the size of baryonic acoustic oscillation peak found in Sloan Digital Sky Survey （SDSS）. In a spatially flat universe case we obtain, at a 95.4% confidence level, A8 = 0.76^＋0.07 -0.07 and α= 0.028^＋0.322 -0.2382 Our results are consistent with the ACDM model （α= 0）, but rule out the standard Chaplygin gas model （α= 1）.
文摘Background: The Mediterranean Diet (MD) has been linked to a reduced risk of developing degenerative diseases, including atherosclerosis, heart stroke, diabetes, arthritis and cancer. However, only a few scientific investigations have attempted to validate this impression. The ingredients of the MD include significant amounts of omega (ω3, ω6, and ω9) unsaturated fatty acids (UFAs). A few studies of these UFAs in the prevention or treatment of arthritis have yielded controversial results, but a general belief regarding their beneficial effects has prevailed. Objective: To investigate the effects of three relevant UFAs, namely Docosahexaenoic Acid (DHA), Arachidonic Acid (AA), and Oleic Acid (OA) (ω3, ω6, and ω9, respectively), in the development of arthritis using a murine model of Collagen-Induced Arthritis (CIA). Methods: DBA-1 mice were immunized with chicken collagen type II (CII) and were subsequently treated with ω-UFAs for 53 days. Dexamethasone (DEXA) was used as a positive anti-inflammatory agent. The effect of the treatments was evaluated through several parameters: inflammation indices, antibody levels, cell prolifera- tion, and histopathological findings. Results and Conclusion: The anti-inflammatory effect of the tested substances was inversely correlated with the histopathological findings: a greater anti- inflammatory effect was associated with less articular damage. Oleic acid (ω9) was the most efficient anti-inflammatory UFA, followed by DHA and then AA. DEXA completely inhibited the development of arthritis, whereas the untreated CII-immunized mice developed the most severe articular damage. DBA-1 mice with CII-induced arthritis constitute an adequate model for the study of arthritis and its treatment.
文摘目的分析低剂量三氯乙烯(TCE)诱导的细胞兴奋效应时,谷胱甘肽转移酶Ω-1(GSTO-1)的表达变化。方法利用前期的双向电泳和质谱结果,参照前期兴奋效应的剂量设计,提取染毒细胞的mRNA和总蛋白,进一步使用实时定量RT-PCR和Western blotting分别从mRNA和蛋白水平上验证GSTO-1的表达变化。结果 Western blotting分析发现,0.1mmol/LTCE剂量组能诱导GSTO-1蛋白表达是对照组的2倍,但随着剂量的增加,蛋白表达改变不明显(与对照组相比,P<0.05)。RT-PCR结果提示:低剂量TCE能诱导GSTO-1基因表达增加,随着剂量的增加,表达反而有所减少。结论低剂量TCE能诱导GSTO-1基因及蛋白表达增加。
