Allograft of developing metanephroi into omenta of cyclosporine-treated adult rats after preservation

To evaluate the animal models and the effect on organogenesis and function of embryonic metanephroi allografted into cyclosporine-treated aduld rats after their preservation in histidine-tryptophan-ketoglutarate (HTK) solution.Methods Whole metanephroi preserved in HTK for 3 days from embryonic day 16 and 17 (E16,E17) of Sprague-Dawley (SD) rats were grouped and allografted into the omenta of cyclosporine-treated SD adult rats which had one kidney resected.E16 metanephroi implanted directly into omenta were used as controls.3 to 4 weeks after implantation,metanephroi allografted in host rats were removed for histopathological and electronic microscopy (EM) examination or anastomosed for renal function measurement 8 weeks later.Results 3 to 4 weeks post-implantation,E16 and E17 metanephroi had formed mature nephrons and collecting ducts with few lympocytic infiltration.EM examination showed they had cellular ultrastructure of normal nephrons and collecting ducts.E16 and E17 metanephroi allografted after preservation for 3 days had no significantly different values of wet weight (P=0.346,P=0.705),urine volume (P=0.396,P=0.687),and creatinine clearances (P=0.469,P=0.543) from those of E16 metanephroi implanted directly.Conclusion E16 and E17 metanephroi allografted into cyclosporine-treated adult rats undergo growth and differentiation and exhibit excretory function after preservation in HTK solution for 3 days.9 refs,1 tabs.

人腹膜间皮细胞的分离培养

目的体外培养的人腹膜间皮细胞（ＨＰＭＣ）是研究腹膜功能的有用模型，我们为此建立了一种改良消化法，以从人大网膜分离腹膜间皮细胞。方法用０．２％胰蛋白酶－０．０２％ＥＤＴＡＮａ２消化人大网膜组织碎片，细胞悬液经１００目筛网滤过后进行培养：瑞士姬姆萨染色观察细胞形态，计算细胞纯度：免疫组化（ＡＢＣ法）鉴定细胞。结果分离培养的细胞光镜下呈铺路鹅卵石状，纯度达９５％以上。细胞表面标志鉴定显示角蛋白、波形蛋白抗原阳性，第Ⅷ因子相关抗原、白细胞ＣＤ４５抗原阴性，证实分离培养的确是ＨＰＭＣ。结论胰蛋白酶消化法是一种简单实用的分离ＨＰＭＣ的方法。

大网膜病变的CT诊断

目的探讨大网膜常见病变的CT影像征象,为临床正确诊断提供依据。方法经病理或临床证实的大网膜病变18例,术前均行多排螺旋CT平扫及增强检查。结果大网膜病变表现为大网膜弥漫浸润性改变的为13例,实性及囊性肿块性病变4例,1例表现为膈疝。大网膜炎性感染性病变10例,肿瘤性病变7例。结论侵犯大网膜的病变种类多样,临床和影像特征具有非特异性和重叠性,需要综合判断提高诊断准确性。

小儿肠系膜、网膜损伤的诊治分析

目的:探讨小儿肠系膜、网膜损伤的诊断和治疗。方法:对9例肠系膜、网膜损伤的临床特点和手术方法进行分析。结果:3例肠系膜损伤、5例网膜损伤治愈。1例胆总管横断伤因误诊多次手术,并发胆汁性肝硬化自动出院。结论:腹部创伤术中应注意探查肠系膜、网膜,根据损伤的部位和程度决定手术方式。

大网膜在实验性大鼠急性坏死性胰腺炎中的作用

对急性坏死性胰腺炎（ＡＮＰ）的机理作进一步探讨。方法：建立大鼠ＡＮＰ模型，同时切除大鼠大网膜。结果：胰性腹水的量减少，腹水中胰酶及毒性物质的含量减低，由此进入血液的毒性物质的含量亦减低。结论：大网膜在ＡＮＰ中的作用不可低估。只要能减少脂肪组织就能减少胰性腹水，减少胰酶及其毒性物质的含量，胰酶的活性也可降低，对周围组织的破坏作用也可减轻。

腹膜后纤维化致肾积水4例报告

目的:探讨腹膜后纤维化的病因、诊断及治疗方法。方法:采用输尿管松解、带蒂大网膜包裹的方法治疗腹膜后纤维化致肾积水4例。结果:随访0.5～3年,4例患者肾功能正常,肾积水基本消失。1例继发性腹膜后纤维化患者因肾积水严重,行患肾切除术,随访3年4个月,对侧肾功能正常。结论:逆行肾盂造影是重要的诊断手段,带蒂大网膜包裹术是有效的治疗方法。