Based on the helix rotating screen and the digital micro-mirror device （DMD）, the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an ...Based on the helix rotating screen and the digital micro-mirror device （DMD）, the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an imaging space meliorate the defects, such as the smaller image space, the fewer voxels and the severer voxel overlap dead zone caused by planar rotating screen. DMD with spatial light modular （SLM） technology increases the transmission bandwidth of 3-D data in the voxel activation subsystem and activate multi-voxel once time. The volumetric-swept system based on helix rotating screen and DMD is developed. The experimental results show that the image space, the vision dead zone, the voxels on slice, and the voxel activation capacity of the designed proto are superior to the plane rotating screen system.展开更多
In this study, a multipurpose M13KE phage display vector was constructed from wild-type M13KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput sc...In this study, a multipurpose M13KE phage display vector was constructed from wild-type M13KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput screening. Based on the relationship between the structure and function of minor coat protein of wild-type MI3KE （wt-plII）, a truncated gene III （tglll） encoding minor coat protein from M13KE phage was cloned. A fusion gene fragment harboring a hw/tac promoter, signal peptide and C-terminal region sequence of gill was assembled with SOEing-PCR （splice-overlapping-extension polymerase chain reaction） method and inserted into M13KE vector. SDS-PAGE and Western blot analysis with anti-M13 pIII moneclonal antibody were employed to detect the expression of re- combinant protein, c-Myc and HA tag sequences were fused into the recombinant protein. The results showed that tglll was inserted into an unessential region of M13KE. According to the results of SDS-PAGE and Western blot with anti-M13 pIII antibody, pIII was expressed by wt-gIII and tgIII, glII harboring two tags ex- pressed both c-Myc and HA peptides using SDS-PAGE and Western blot with the corresponding monoclonal antibodies. In this study, a multipurpose M13KE phage display system was successfully constructed, which could express both short and long peptide libraries without helper phage. In future, the obtained M13KE phage display system may be used for targeted high-throughput screening of long peptide libraries without helper phage.展开更多
Designing military screen (display) is not complicated in the past, the design should contains easiness of using, visibility and functionality. And it is important that contains all of the information what the system ...Designing military screen (display) is not complicated in the past, the design should contains easiness of using, visibility and functionality. And it is important that contains all of the information what the system could be gotten. Today military display design can be valued by consideration of human’s cognition model. A few information, which operators of military display could easily miss, could bring critical loss. So the military display needs the ability to help operator decide the military decision with sufficient consideration of battlefield information in a short time. The information is considerably complicated because it has many military entities and many attributes for each entities. For this reason this research contains the methodology of information organization especially for visual information. When the operator use military display, the required information should be recognized at a glance and located where the operator can place his eye easily. Therefore, the display screen should efficiently organize and effectively transfer the information. This paper specifically proposes design methodology to effectively organize and visualize the information in the display screen. The theory of information visualization in a variety of fields already exist, but it has no established theory for the origin of the display screen design. Since the theory was selected to organize the military display interface design for the display screen of the battlefield situation existing information visualization theory.展开更多
介绍1款视频处理ASIC芯片中OSD系统的设计方案,先读取OSD内部SRAM中的属性和字符内容,然后控制电路根据属性将字符内容以RGB的形式表现出来,最后将OSD信息与视频内容进行α混合,从而可以在屏幕上得到OSD信息。该OSD系统用户自定义性强,...介绍1款视频处理ASIC芯片中OSD系统的设计方案,先读取OSD内部SRAM中的属性和字符内容,然后控制电路根据属性将字符内容以RGB的形式表现出来,最后将OSD信息与视频内容进行α混合,从而可以在屏幕上得到OSD信息。该OSD系统用户自定义性强,使用改进的内部SRAM中1/2/4像素深度(BPP,Bits Per Pixel)字符或图形点阵的存储结构以提高字符或图形存储量,其具有闪烁、阴影、透明、单个字符宽高加倍、非线性放大、可编程的行列数和颜色等诸多功能。芯片在UMC0.18μm CMOS工艺下流片,其OSD系统的工作频率可达170 MHz。展开更多
Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhI...Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhIFNa2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNa2b.RhIFNa2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNa2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNa2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNa2b detection assay had a limit of detection of 1 mg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other commercial products,with a great potential for routine use in detecting counterfeit recombinant protein products.展开更多
A novel see-through display with a liquid crystal lens array was proposed.A liquid crystal Fresnel lens display（LCFLD） with a holographic screen was demonstrated.The proposed display system has high efficiency,simpl...A novel see-through display with a liquid crystal lens array was proposed.A liquid crystal Fresnel lens display（LCFLD） with a holographic screen was demonstrated.The proposed display system has high efficiency,simple fabrication,and low manufacturing cost due to the absence of a polarizer and color filter.展开更多
This thesis paper is a brief summary of user centered design, (particularly in the situation of under the battlefield), which incorporates the related information after reviewing many other papers. A display screen us...This thesis paper is a brief summary of user centered design, (particularly in the situation of under the battlefield), which incorporates the related information after reviewing many other papers. A display screen user requires an ability to provide proper information while considering a variety of information in a short period. While operating the display screen, the user is heavily pressed for time as the missions are allocated through many different steps of orders. In addition, since a failure would cause fatal damage or cause further defeat, this increases the pressure of time. The right environment for operating a display screen is most useful when the user is in the most vulnerable time period or long-term continuous operation of the display screen. In that kind of sterile environment, the operating display screen is there to help the user’s decisions for the matter at hand, in this it should follow the user centered design and improve the user’s handiness and use. Furthermore, the information should be provided in accordance with the user's informational and perceptional process, and the capacity of the information process should be improved with the movement through each process and step. This thesis paper can be used as a reference material when designing the display screen in order to apply a user’s experiences.展开更多
The satellite laser ranging （SLR） data quality from the COMPASS was analyzed, and the difference between curve recognition in computer vision and pre-process of SLR data finally proposed a new algorithm for SLR was ...The satellite laser ranging （SLR） data quality from the COMPASS was analyzed, and the difference between curve recognition in computer vision and pre-process of SLR data finally proposed a new algorithm for SLR was discussed data based on curve recognition from points cloud is proposed. The results obtained by the new algorithm are 85 % （or even higher） consistent with that of the screen displaying method, furthermore, the new method can process SLR data automatically, which makes it possible to be used in the development of the COMPASS navigation system.展开更多
In this Letter,we present a display system based on a curved screen and parallax barrier,which provides stereo images with a horizontal field of view of 360°without wearing any eyewear,to achieve an immersive aut...In this Letter,we present a display system based on a curved screen and parallax barrier,which provides stereo images with a horizontal field of view of 360°without wearing any eyewear,to achieve an immersive autostereoscopic effect.The display principle and characteristics of this display system are studied theoretically in detail.Three consecutive pixels on a curved screen and parallax barrier form a display unit,which can generate separate viewing zones for the left and right eyes,respectively.Simulation and experimental results show that the non-crosstalk effect can be obtained in the viewing zones,which proves the effectiveness of this display system.This study provides some new ideas for the improvement of the autostereoscopic display and to enable envisioned applications in virtual reality technology.展开更多
基金Supported by the National High Technology Research and Development Program of China(″863″Program)(2007AA01Z338)the National Science Foundation for Post-doctoral Scientists of China(20080441051)the Jiangsu Province Science Foundation for Post-doctoral Scientists(0802014c)~~
文摘Based on the helix rotating screen and the digital micro-mirror device （DMD）, the former proto of volumetric-swept display system is improved. The 3-D display system adopting a helix rotating screen to construct an imaging space meliorate the defects, such as the smaller image space, the fewer voxels and the severer voxel overlap dead zone caused by planar rotating screen. DMD with spatial light modular （SLM） technology increases the transmission bandwidth of 3-D data in the voxel activation subsystem and activate multi-voxel once time. The volumetric-swept system based on helix rotating screen and DMD is developed. The experimental results show that the image space, the vision dead zone, the voxels on slice, and the voxel activation capacity of the designed proto are superior to the plane rotating screen system.
基金Supported by Youth Fund of Suzhou Chien-shiung Institute of Technology(2013QNJJ38)
文摘In this study, a multipurpose M13KE phage display vector was constructed from wild-type M13KE phage for long peptide or protein display libraries without helper phage to expand the scope of targeted high-throughput screening. Based on the relationship between the structure and function of minor coat protein of wild-type MI3KE （wt-plII）, a truncated gene III （tglll） encoding minor coat protein from M13KE phage was cloned. A fusion gene fragment harboring a hw/tac promoter, signal peptide and C-terminal region sequence of gill was assembled with SOEing-PCR （splice-overlapping-extension polymerase chain reaction） method and inserted into M13KE vector. SDS-PAGE and Western blot analysis with anti-M13 pIII moneclonal antibody were employed to detect the expression of re- combinant protein, c-Myc and HA tag sequences were fused into the recombinant protein. The results showed that tglll was inserted into an unessential region of M13KE. According to the results of SDS-PAGE and Western blot with anti-M13 pIII antibody, pIII was expressed by wt-gIII and tgIII, glII harboring two tags ex- pressed both c-Myc and HA peptides using SDS-PAGE and Western blot with the corresponding monoclonal antibodies. In this study, a multipurpose M13KE phage display system was successfully constructed, which could express both short and long peptide libraries without helper phage. In future, the obtained M13KE phage display system may be used for targeted high-throughput screening of long peptide libraries without helper phage.
文摘Designing military screen (display) is not complicated in the past, the design should contains easiness of using, visibility and functionality. And it is important that contains all of the information what the system could be gotten. Today military display design can be valued by consideration of human’s cognition model. A few information, which operators of military display could easily miss, could bring critical loss. So the military display needs the ability to help operator decide the military decision with sufficient consideration of battlefield information in a short time. The information is considerably complicated because it has many military entities and many attributes for each entities. For this reason this research contains the methodology of information organization especially for visual information. When the operator use military display, the required information should be recognized at a glance and located where the operator can place his eye easily. Therefore, the display screen should efficiently organize and effectively transfer the information. This paper specifically proposes design methodology to effectively organize and visualize the information in the display screen. The theory of information visualization in a variety of fields already exist, but it has no established theory for the origin of the display screen design. Since the theory was selected to organize the military display interface design for the display screen of the battlefield situation existing information visualization theory.
文摘介绍1款视频处理ASIC芯片中OSD系统的设计方案,先读取OSD内部SRAM中的属性和字符内容,然后控制电路根据属性将字符内容以RGB的形式表现出来,最后将OSD信息与视频内容进行α混合,从而可以在屏幕上得到OSD信息。该OSD系统用户自定义性强,使用改进的内部SRAM中1/2/4像素深度(BPP,Bits Per Pixel)字符或图形点阵的存储结构以提高字符或图形存储量,其具有闪烁、阴影、透明、单个字符宽高加倍、非线性放大、可编程的行列数和颜色等诸多功能。芯片在UMC0.18μm CMOS工艺下流片,其OSD系统的工作频率可达170 MHz。
基金support was provided by the National Science and Technology Major Project(Grant No.:2015ZX09501008)。
文摘Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhIFNa2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNa2b.RhIFNa2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNa2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNa2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNa2b detection assay had a limit of detection of 1 mg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other commercial products,with a great potential for routine use in detecting counterfeit recombinant protein products.
基金Project supported by Partner State Key Laboratory on Advanced Displays and Optoelectronics Technologies HKUST,Chinathe National Natural Science Foundation of China(Grant Nos.61435008 and 61575063)the Fundamental Research Funds for the Central Universities,China(Grant No.WM1514036)
文摘A novel see-through display with a liquid crystal lens array was proposed.A liquid crystal Fresnel lens display（LCFLD） with a holographic screen was demonstrated.The proposed display system has high efficiency,simple fabrication,and low manufacturing cost due to the absence of a polarizer and color filter.
文摘This thesis paper is a brief summary of user centered design, (particularly in the situation of under the battlefield), which incorporates the related information after reviewing many other papers. A display screen user requires an ability to provide proper information while considering a variety of information in a short period. While operating the display screen, the user is heavily pressed for time as the missions are allocated through many different steps of orders. In addition, since a failure would cause fatal damage or cause further defeat, this increases the pressure of time. The right environment for operating a display screen is most useful when the user is in the most vulnerable time period or long-term continuous operation of the display screen. In that kind of sterile environment, the operating display screen is there to help the user’s decisions for the matter at hand, in this it should follow the user centered design and improve the user’s handiness and use. Furthermore, the information should be provided in accordance with the user's informational and perceptional process, and the capacity of the information process should be improved with the movement through each process and step. This thesis paper can be used as a reference material when designing the display screen in order to apply a user’s experiences.
文摘The satellite laser ranging （SLR） data quality from the COMPASS was analyzed, and the difference between curve recognition in computer vision and pre-process of SLR data finally proposed a new algorithm for SLR was discussed data based on curve recognition from points cloud is proposed. The results obtained by the new algorithm are 85 % （or even higher） consistent with that of the screen displaying method, furthermore, the new method can process SLR data automatically, which makes it possible to be used in the development of the COMPASS navigation system.
基金supported by the Youth Innovation Promotion Association,Chinese Academy of Sciences(Nos.2018251 and 2017264)the National Natural Science Foundation of China(Nos.11704378 and 61705221)。
文摘In this Letter,we present a display system based on a curved screen and parallax barrier,which provides stereo images with a horizontal field of view of 360°without wearing any eyewear,to achieve an immersive autostereoscopic effect.The display principle and characteristics of this display system are studied theoretically in detail.Three consecutive pixels on a curved screen and parallax barrier form a display unit,which can generate separate viewing zones for the left and right eyes,respectively.Simulation and experimental results show that the non-crosstalk effect can be obtained in the viewing zones,which proves the effectiveness of this display system.This study provides some new ideas for the improvement of the autostereoscopic display and to enable envisioned applications in virtual reality technology.
