UV/Vis-based process analytical technology to improve monoclonal antibody and host cell protein separation

Process analytical technology(PAT) is gaining more interest in the biomanufacturing industry because of its potential to improve operational control and compliance through real-time quality assurance.Currently, biopharmaceutical producers mainly monitor chromatographic processes with ultraviolet/visible(UV/Vis) absorbance. However, this measurement has a very limited correlation with purity and quantity. The current study aims to determine the concentration of monoclonal antibody(mAb) and host cell proteins(HCPs) using a build-in UV/Vis monitoring during Protein A affinity chromatography and to optimize the separation conditions for high purity of mAb and minimizing the HCPs content. The eluate was analyzed through in-line UV/Vis at 280 and 410 nm, representing mAb and HCPs concentration,respectively. Each 0.1 column volume(CV) fraction of UV/Vis chromatogram peak area were calculated,and different separation conditions were then compared. The optimum conditions of mAb separation were found as 12 CV loading, elution at pH 3.5, and starting the collection at 0.5 CV point, resulting in high m Ab recovery of 95.92% and additional removal of 49.98% of HCP comparing with whole elution pool. This study concluded that UV/Vis-based in-line monitoring at 280 and 410 nm showed a high potential to optimize and real-time control Protein A affinity chromatography for mAb purification from HCPs.

Design and development of analytical CRM based on OLAM technology

Some key technologies of actualizing customer relationship management(CRM)systems are researched.The customer analysis mining prototype systems on the basis of on-line analytical mining(OLAM)is designed.After transaction analysis,the data warehouse of CRM is constructed.The CLV/CL/CC customer division model based on customer lifetime value,customer loyalty and customer credit is emphatically researched.Three parameters of customer division—customer lifetime value,customer loyalty and customer credit—are calculated by corresponding algorithms,which can realize customer divisions effectively and improve the accuracy of distinguishing among customers.The data of product sales are analyzed by the sequence association rules algorithm,the potential rules of the products relevance are discovered,which can provide evidence for supporting decisions such as promotion strategies.The transaction data such as product sales volumes and order lists are analyzed on-line through multi-dimensional and multi-level up-drills,down-drills,and horizontal/longitudinal sections.The customer property factors are analyzed as well.The theory and practice of OLAM and its visualization are further explored.

Raman spectroscopy as process analytical tool in downstream processing of biotechnology

Downstream processing or product recovery plays a vital role in the development of bioprocesses.To improve the bioprocess efficiency,some unconventional methods are much required.The continuous manufacturing in downstream processing makes the Process Analytical Technologies(PATs)as an important tool.Monitoring and controlling bioprocess are an essential factor for the principles of PAT and quality by design.Spectroscopic methods can apply to monitor multiple analytes in real-time with less sample processing with significant advancements.Raman spectroscopy is an extensively used technique as an analytical and research tool owing to its modest process form,non-destructive,non-invasive optical molecular spectroscopic imaging with computer-based analysis.Generally,its application is essential for the analysis and characterization of biological samples,and it is easy to operate with minimal sample.The innovation on various types of enhanced Raman spectroscopy was designed to enhance the Raman analytical technique.Raman spectroscopy could couple with chemometrics to provide reliable alternative analysis method of downstream process analysis.Thus,this review aims to provide useful insight on the application of Raman spectroscopy for PAT in downstream processing of biotechnology and Raman data analysis in biological fields.

Research Progress of On-line Monitoring Technology for Electromagnetic Environment of Power Transmission and Transformation Projects

Based on the collection of relevant literature and cases,the research and application status of on-line monitoring technology for electromagnetic environment of power transmission and transformation projects at home and abroad were introduced.Moreover,the problems existing in the on-line monitoring of electromagnetic environment were expounded,and the development prospect was forecasted.

Microfluidic Analytical System with On-Line Luminol Chemiluminescence Detection Based on Annular Flow of Phase Separation Multiphase Flow

Microfluidic analytical system was developed based on annular flow of phase separation multiphase flow with a ternary water-hydrophilic/hydrophobic organic solvent solution. The analytical system was combined with on-line luminol chemiluminescence detection for catechin analysis. The water (10 mM phosphate buffer, pH 7.3)-acetonitrile-ethyl acetate mixed solution (3:8:4, volume ratio) containing 60 μM luminol and 2 mM hydrogen peroxide as a carrier was fed into the capillary tube (open-tubular fused-silica, 75 μm inner diameter, 110 cm effective length) at a flow rate of 1.0 μL·min-1. The carrier solution showed stable chemiluminescence as a baseline on the flow chart. Eight catechins were detected as negative peaks for their antioxidant potential with different detection times. The system was applied to analyze the amounts of catechin in commercially available green tea beverages.

Coupling of on-Line Pre-Column Oxidative Cleavage and Solid-Phase Enrichment with Liquid Chromatography Using an Eco-Friendly Analytical Procedure to Determine Low Levels of Methotrexate

A simple, sensitive and precise green high-performance liquid chromatographic method including on-line pre-column oxidation combined by column switching with a short Hypersil ODS analytical column (100 mm × 4.0 mm i.d.) for enrichment and separation was developed and validated to determine low levels of methotrexate (MTX). The method was based on oxidative cleavage of MTX into highly fluorescence products, 2,4-diaminopteridine-6-carboxaldehyde and the corresponding 2,4-diaminopteridine-6-carboxylic acid, during the flow of phosphate buffer (0.04 M, pH 3.4) containing the analyte through the packed reactor of cerium (IV) trihydroxyhydroperoxide (CTH) at a flow-rate of 0.2 mL/min and 40℃. The fluorescent products were enriched on the head of ODS analytical column for the final separation. The separation was performed at room temperature using an environmentally friendly mobile phase consisting of ethanol and phosphate buffer (0.04 M, pH 3.4) in the ratio of 10:90 (v/v). The eluent was monitored at emission and excitation wavelengths of 463 and 367 nm, respectively. The method was successfully applied, without any interference from the excipients, for the determination of drug in tablets and vials with a detection limit of 0.06 ng/mL from 500 ?L of sample MTX.

Privacy-preserving analytics for the securitization market: a zero-knowledge distributed ledger technology application

A zero-knowledge proof or protocol is a cryptographic technique for verifying private data without revealing it in its clear form.In this paper,we evaluate the potential for zero-knowledge distributed ledger technology to alleviate asymmetry of information in the asset-backed securitization market.To frame this inquiry,we conducted market data analyses,a review of prior literature,stakeholder interviews with investors,originators and security issuers and collaboration with blockchain engineers and researchers.We introduce a new system which could enable all market participants in the securitization lifecycle(e.g.investors,rating agencies,regulators and security issuers)to interact on a unique decentralized platform while maintaining the privacy of loan-level data,therefore providing the industry with timely analytics and performance data.Our platform is powered by zkLedger(Narula et al.2018),a zero-knowledge protocol developed by the MIT Media Lab and the first system that enables participants of a distributed ledger to run publicly verifiable analytics on masked data.

A review on edge analytics:Issues,challenges,opportunities,promises,future directions,and applications

Edge technology aims to bring cloud resources(specifically,the computation,storage,and network)to the closed proximity of the edge devices,i.e.,smart devices where the data are produced and consumed.Embedding computing and application in edge devices lead to emerging of two new concepts in edge technology:edge computing and edge analytics.Edge analytics uses some techniques or algorithms to analyse the data generated by the edge devices.With the emerging of edge analytics,the edge devices have become a complete set.Currently,edge analytics is unable to provide full support to the analytic techniques.The edge devices cannot execute advanced and sophisticated analytic algorithms following various constraints such as limited power supply,small memory size,limited resources,etc.This article aims to provide a detailed discussion on edge analytics.The key contributions of the paper are as follows-a clear explanation to distinguish between the three concepts of edge technology:edge devices,edge computing,and edge analytics,along with their issues.In addition,the article discusses the implementation of edge analytics to solve many problems and applications in various areas such as retail,agriculture,industry,and healthcare.Moreover,the research papers of the state-of-the-art edge analytics are rigorously reviewed in this article to explore the existing issues,emerging challenges,research opportunities and their directions,and applications.

Study progression in application of process analytical technologies on film coating

Film coating is an important unit operation to produce solid dosage forms,thereby,the monitoring of this process is helpful to find problems in time and improve the quality of coated products.Traditional methods adopted to monitor this process include measurement of coating weight gain,performance of disintegration and dissolution test,etc.However,not only do these methods cause destruction to the samples,but also consume time and energy.There have recently emerged the applications of process analytical technologies(PAT)on film coating,especially some novel spectroscopic and imaging technologies,which have the potential to real-time track the progress in film coating and optimize production efficiency.This article gives an overview on the application of such technologies for film coating,with the goal to provide a reference for the further researches.

Applications and Challenges of Smart Technologies in Supply Chain Management

The advent of intelligent technologies in supply chain management(SCM)through the use of artificial intelligence(AI),the Internet of Things(IoT),blockchain,big data analytics,and others is a game changer.These improvements increase clarity,effectiveness,and flexibility,allowing companies to quickly deal with market changes and interruptions.Nevertheless,adopting such technologies comes with difficulties,such as information security,compatibility,exorbitant prices,and employee opposition.The applications of intelligent technologies for SCM are examined in this article,as well as the importance of their implementation and solutions that provide a competitive advantage for businesses.

MUETEK Analytic GmbH和BTG Pulp & Paper Technology合并业务

BTG浆纸科技公司(BTG Pulp ＆ Paper Technolo-gy)于2001年1月16日,以其在瑞士Eclpens的集团总部取得了德国Muetek Analytic BmbH多数股权。BTG聘请Ms.Lydia Bley和Mr.Alfred

Process analytical technologies and self-optimization algorithms in automated pharmaceutical continuous manufacturing

The pharmaceutical industry is now paying increased attention to continuous manufacturing.While the revolution to continuous and automated manufacturing is deepening in most of the top pharma companies in the world,the advancement of automated pharmaceutical continuous manufacturing in China is relatively slow due to some key challenges including the lack of knowledge on the related technologies and shortage of qualified personnels.In this review,emphasis is given to two of the crucial technologies in automated pharmaceutical continuous manufacturing,i.e.,process analytical technology(PAT)and self-optimizing algorithm.Research work published in recent 5 years employing advanced PAT tools and self-optimization algorithms is introduced,which represents the great progress that has been made in automated pharmaceutical continuous manufacturing.

Review on recent progress in on-line monitoring technology for atmospheric pollution source emissions in China

Emissions from mobile sources and stationary sources contribute to atmospheric pollution in China,and its components,which include ultrafine particles(UFPs),volatile organic compounds(VOCs),and other reactive gases,such as NH3and NOx,are the most harmful to human health.China has released various regulations and standards to address pollution from mobile and stationary sources.Thus,it is urgent to develop online monitoring technology for atmospheric pollution source emissions.This study provides an overview of the main progress in mobile and stationary source monitoring technology in China and describes the comprehensive application of some typical instruments in vital areas in recent years.These instruments have been applied to monitor emissions from motor vehicles,ships,airports,the chemical industry,and electric power generation.Not only has the level of atmospheric environment monitoring technology and equipment been improving,but relevant regulations and standards have also been constantly updated.Meanwhile,the developed instruments can provide scientific assistance for the successful implementation of regulations.According to the potential problem areas in atmospheric pollution in China,some research hotspots and future trends of atmospheric online monitoring technology are summarized.Furthermore,more advanced atmospheric online monitoring technology will contribute to a comprehensive understanding of atmospheric pollution and improve environmental monitoring capacity.

Processing, Recovery and Analysis of Precious Metals Using Molecular Recogntion Technology

IBC Advanced Technologies' Molecular Recognition Technology(MRT) products,trade named SuperLig,selectively and rapidly bind with target metal ions to remove them from solution.The MRT process can produce a high purity separation product of maximum added value at low cost.In this paper,applications of MRT in the precious metals industry,including selective commercial separations involving Au,Pd,Pt,Rh,and Ru,are described and discussed.Application of MRT to the analytical determination of precious metals is presented.Potential use of MRT in recovering precious metals from end-of-life(EOL) products is discussed.

The Multiplicative Analytic Hierarchy Process (MIAHP) as a Quality Criterion Determining the Technological Value of the Egyptian Cotton Varieties

This study was undertaken to develop a numerical process that can be used as a quality criterion to determine the technological value of the Egyptian cotton varieties, which in turn would denote the end-use of their fibers. However the material used in the study comprised the 6 Egyptian cotton varieties Giza70, Giza80, Giza86, Giza88, Giza90 and Giza92. According to the local practice in Egypt, Giza70, Giza88 and Giza92 belong to the Extra-Long Staple (ELS) category, while Giza80, Giza86 and Giza90 are included under the Long Staple (LS) category. The regression analysis of the relationships between fiber properties and yarn skein strength (lea product) of the 2 carded ring counts 40 and 50 Ne, was employed to drive an equation for calculating the Multiplicative Analytic Hierarchy Process (MIAHP) values. The values of the MIAHP have been used as numerical determinations of the technological values of the Egyptian cotton varieties. Nevertheless, the findings of this study clarified that with respect to the criteria weights, the pair-wise comparisons denoted that fiber length properties of Egyptian cotton ranked first where they revealed the most dominant effect on yarn strength, while tensile properties ranked second with a relative weight close to that of fiber length. On the contrary, the relative weight of fiber fineness (micronaire reading) was found to be marginal. With regard to the relative weight of sub-criterion, the pair-wise comparisons indicated that the role of fiber tenacity as a determinant of yarn strength is much superior to that of fiber elongation. Further the global weights of the sub-criterion of fiber length pointed out that the UHML (upper half mean length) plays an important role in determining yarn strength of the Egyptian cotton comparing with either the UI (uniformity index) or the SFC (short fiber content). In conformity with the values of the MIAHP, it was found that in the order of descending rank, Giza88 ranked first, followed by Giza92, Giza70, Giza86, Giza80 and finally Giza90.

An Analytical Model for the Electrolyser Performance Derived from Materials Parameters

Hydrogen is seen as a key element for the transition from a fossil fuel based economy to a renewable, sustainable economy. Hydrogen can be used either directly as an energy carrier or as a feedstock for the reduction of CO2 to synthetic hydrocarbons. Hydrogen can be produced by electrolysis, decomposing water in oxygen and hydrogen. This paper presents an overview of the three major electrolysis technologies: acidic (PEM), alkaline (AEL) and solid oxide electrolysis (SOEC). An updated list of existing electrolysers and commercial providers is provided. Most interestingly, the specific prices of commercial devices are also given when available. Despite tremendous development of the PEM technology in the past decades, the largest and most efficient electrolysers are still alkaline. Thus, this technology is expected to play a key role in the transition to the hydrogen society. A detailed description of the components in an alkaline electrolyser and an analytical model of the process are provided. The analytical model allows investigating the influence of the different operating parameters on the efficiency. Specifically, the effect of temperature on the electrolyte conductivity—and thus on the efficiency—is analyzed. It is found that in the typical range of operating temperatures for alkaline electrolysers of 65°C - 220°C, the efficiency varies by up to 3.5 percentage points, increasing from 80% to 83.5% at 65°C and 220°C, respectively.

Using the Analytical Hierarchy Process Model in the Prioritization of Information Assurance Defense In-Depth Measures?—A Quantitative Study

Organizational computing devices are increasingly becoming targets of cyber-attacks, and organizations have become dependent on the safety and security of their computer networks and their organizational computing devices. Business and government often use defense in-depth information assurance measures such as firewalls, intrusion detection systems, and password procedures across their enterprises to plan strategically and manage IT security risks. This quantitative study explores whether the analytical hierarchy process (AHP) model can be effectively applied to the prioritization of information assurance defense in-depth measures. In response to these threats, the President, legislators, experts, and others have characterized cyber security as a pressing national security issue. The methods used in this study consisted of emailing study participants a survey requesting that they prioritize five defense in-depth information assurance measures, anti-virus, intrusion detection, password, smart-cards, and encryption, with a range of responses from 1 - 5 using a Likert scale to consider standard cost, effectiveness, and perceived ease of use in terms of protection of organizational computing devices. The measures were then weighted, based on ranking. A pair-wise comparison of each of the five measures is then made using AHP to determine whether the Likert scale and the AHP model could be effectively applied to the prioritization of information assurance measures to protect organizational computing devices. The findings of the research reject the H0 null hypothesis that AHP does not affect the relationship between the information technology analysts’ prioritization of five defense in-depth dependent variables and the independent variables of cost, ease of use, and effectiveness in protecting organizational devices against cyber-attacks.

Understanding RFID Technology within a Business Intelligence Framework

Radio Frequency Identification (RFID) technology provides new and exciting opportunities for increasing organiza- tional, financial, and operational performance. With its focus on organizational efficiency and effectiveness, RFID technology is superior to barcodes in its ability to provide source automation features that increase the speed and volume of data collection for analysis. Today, applications that employ RFID are growing rapidly and this technology is in a continuous state of evolution and growth. As it continues to progress, RFID provides us with new opportunities to use business intelligence (BI) to monitor organizational operations and learn more about markets, as well as consumer attitudes, behaviors, and product preferences. This technology can even be used to prevent potentially faulty or spoiled products from ending up in the hands of consumers. However, RFID offers significant challenges to organizations that attempt to employ this technology. Most significantly, there exists the potential for RFID to overwhelm data collection and BI analytic efforts if organizations fail to effectively address RFID data integration issues. To this end, the purpose of this article is to explicate the dynamic technology of RFID and how it is being used today. Additionally, this article will provide insights into how RFID technology is evolving and how this technology relates to BI and issues related to data integration. This knowledge has never been more essential. While IT academic research into RFID development and issues has declined in recent years, RFID continues to be a vital area of exploration, especially as it relates to BI in the 21st century.

Transformation and risk assessment of the East Kolkata Wetlands (India)using fuzzy MCDM method and geospatial technology

The East Kolkata Wetlands (EKW) is located on the eastern periphery of the city of Kolkata and extends up to theBidyadhari-Matla River confluence. It is a Ramsar Site and acts as an absorber basin for a large number of con-taminants drained from Kolkata. Agricultural lands, sewage-fed fisheries, garbage dumping fields, horticulture,and built-up areas are included in this protected area, that covers approximately 125 km2. It reveals that climatechange reduces the variety of wetland ecosystem services and increases socio-economic vulnerability and eco-nomic stress. The human encroachment, reclamation of land for agriculture, aquaculture, and urban expansion inand around Kolkata has recently adversely threatened the EKW. The remotely sensed data, socio-economic data,and responses of inhabitants have been used to analyse the EKW’s risk and vulnerability. We employed geospatialanalysis by using the Multi-Criteria Decision Making (MCDM) method using nine risk factors. An in-depth analysisof the EKW using geospatial techniques and the Fuzzy Analytic Hierarchy Process (FAHP) helped to understandthe EKW transformations through vulnerability and risk analysis. The results show that the transformation of thewetland to aquaculture, eutrophication and pollution, road proximity, waste dumping, population density, andgrowth are the main factors for the deteriorating health, quality, and environment of the EKW. It also reveals thatquantitative and qualitative evaluations of ecosystem services, wetland degradation, transformation, and cause-effect rapport should be periodically assessed using scientific methods like FAHP, RS, GIS to formulate resilient,integrated plans and strategy for the sustainable management of the EKW.

Application of Industry： A AHP Method to Technolo Selection for Emerging Case Study of Chinese Biotechnology Enterprises

Selecting the promising technologies to incorporate in new products is challenging for enterprises, especially for those who compete in emerging high-technology industries. By integrating external factor evaluation （EFE） and internal factor evaluation （1FE） matrix, this study proposes a new technology selection method. The proposed fi＇am^work is applied to a real technology selection problem faced by a bio-technology enterprise in the context of China＇ s economic, political and social environment. Two major aspects and 24 external/internal factors are determined. This study uses analytic hierarchy process （AHP） method to sort the information and derives weights of each criterion. Empirical results indicate that the candidate technology is promising, and shall be developed as one of the enterprise＇s primary products.