A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Cheesy material on macroscopic on-site evaluation after endoscopic ultrasound-guided fine-needle biopsy:Don't miss the tuberculosis

Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)is an excellent investigation to diagnose pancreatic lesions and has shown high accuracy for its use in pathologic diagnosis.Recently,macroscopic on-site evaluation(MOSE)performed by an endoscopist was introduced as an alternative to rapid on-site cytologic evaluation to increase the diagnostic yield of EUS-FNB.The MOSE of the biopsy can estimate the adequacy of the sample directly by the macroscopic evaluation of the core tissue obtained from EUS-FNB.Isolated pancreatic tuberculosis is extremely rare and difficult to diagnose because of its non-specific signs and symptoms.Therefore,this challenging diagnosis is based on endoscopy,imaging,and the bacteriological and histological examination of tissue biopsies.This uncommon presentation of tuberculosis can be revealed as pancreatic mass mimicking cancer.EUS-FNB can be very useful in providing a valuable histopathological diagnosis.A calcified lesion with a cheesy core in MOSE must be suggestive of tuberculosis,leading to the request of the GeneXpert,which can detect Mycobacterium tuberculosis deoxyribonucleic acid and resistance to rifampicin.A decent diagnostic strategy is crucial to prevent unnecessary surgical resection and to supply conservative management with antitubercular therapy.

Influence of initial check, information exchange, final accuracy check, reaction information nursing on the psychology of elderly with lung cancer

BACKGROUND As one of the fatal diseases with high incidence,lung cancer has seriously endangered public health and safety.Elderly patients usually have poor self-care and are more likely to show a series of psychological problems.AIM To investigate the effectiveness of the initial check,information exchange,final accuracy check,reaction(IIFAR)information care model on the mental health status of elderly patients with lung cancer.METHODS This study is a single-centre study.We randomly recruited 60 elderly patients with lung cancer who attended our hospital from January 2021 to January 2022.These elderly patients with lung cancer were randomly divided into two groups,with the control group taking the conventional propaganda and education and the observation group taking the IIFAR information care model based on the conventional care protocol.The differences in psychological distress,anxiety and depression,life quality,fatigue,and the locus of control in psychology were compared between these two groups,and the causes of psychological distress were analyzed.RESULTS After the intervention,Distress Thermometer,Hospital Anxiety and Depression Scale(HADS)for anxiety and the HADS for depression,Revised Piper’s Fatigue Scale,and Chance Health Locus of Control scores were lower in the observation group compared to the pre-intervention period in the same group and were significantly lower in the observation group compared to those of the control group(P<0.05).After the intervention,Quality of Life Questionnaire Core 30(QLQ-C30),Internal Health Locus of Control,and Powerful Others Health Locus of Control scores were significantly higher in the observation and the control groups compared to the pre-intervention period in their same group,and QLQ-C30 scores were significantly higher in the observation group compared to those of the control group(P<0.05).CONCLUSION The IIFAR information care model can help elderly patients with lung cancer by reducing their anxiety and depression,psychological distress,and fatigue,improving their tendencies on the locus of control in psychology,and enhancing their life qualities.

Computation Tree Logic Model Checking of Multi-Agent Systems Based on Fuzzy Epistemic Interpreted Systems

Model checking is an automated formal verification method to verify whether epistemic multi-agent systems adhere to property specifications.Although there is an extensive literature on qualitative properties such as safety and liveness,there is still a lack of quantitative and uncertain property verifications for these systems.In uncertain environments,agents must make judicious decisions based on subjective epistemic.To verify epistemic and measurable properties in multi-agent systems,this paper extends fuzzy computation tree logic by introducing epistemic modalities and proposing a new Fuzzy Computation Tree Logic of Knowledge(FCTLK).We represent fuzzy multi-agent systems as distributed knowledge bases with fuzzy epistemic interpreted systems.In addition,we provide a transformation algorithm from fuzzy epistemic interpreted systems to fuzzy Kripke structures,as well as transformation rules from FCTLK formulas to Fuzzy Computation Tree Logic(FCTL)formulas.Accordingly,we transform the FCTLK model checking problem into the FCTL model checking.This enables the verification of FCTLK formulas by using the fuzzy model checking algorithm of FCTL without additional computational overheads.Finally,we present correctness proofs and complexity analyses of the proposed algorithms.Additionally,we further illustrate the practical application of our approach through an example of a train control system.

层厚对使用CT/SIM Check模体联合iQA软件检测CT模拟机的影响

目的探讨层厚大小对使用CT/SIM Check模体联合iQA软件检测放疗CT模拟机数据结果的影响。方法对1台西门子Sensation Open CT模拟机,使用6种放疗扫描协议扫描1套CT/SIM Check模体,扫描完成后重建1.5、2、3、4和5 mm共5种层厚的CT图像序列。使用iQA软件对这些CT图像序列进行自动分析并生成报表,对报表上的质控数据结果进行分析和比较。结果通过对报表数据进行分析,得到了6种扫描协议和5种层厚情形下22种参数的质控数据,对5种不同层厚的数据计算得到了相应的四分位数间距值。由数据结果可见,HU均一性和插件4内标准差的数据随层厚的增加而减小;扫描中心纵向坐标的数值随层厚的增加有下降的趋势;线宽比-灵敏度截面的数据随层厚的增加而变大。结论使用CT/SIM Check模体联合iQA软件检测CT模拟机,层厚对HU均一性、插件4内标准差、扫描中心纵向坐标和线宽比-灵敏度截面的质控数据结果有较大影响,对其他参数的数据也有不同程度的影响。本研究为物理师使用CT/SIM Check模体联合iQA软件检测放疗CT模拟机的质控工作,提供了有益的数据参考。

Improved edge lightweight YOLOv4 and its application in on-site power system work

A“cloud-edge-end”collaborative system architecture is adopted for real-time security management of power system on-site work,and mobile edge computing equipment utilizes lightweight intelligent recognition algorithms for on-site risk assessment and alert.Owing to its lightweight and fast speed,YOLOv4-Tiny is often deployed on edge computing equipment for real-time video stream detection;however,its accuracy is relatively low.This study proposes an improved YOLOv4-Tiny algorithm based on attention mechanism and optimized training methods,achieving higher accuracy without compromising the speed.Specifically,a convolution block attention module branch is added to the backbone network to enhance the feature extraction capability and an efficient channel attention mechanism is added in the neck network to improve feature utilization.Moreover,three optimized training methods:transfer learning,mosaic data augmentation,and label smoothing are used to improve the training effect of this improved algorithm.Finally,an edge computing equipment experimental platform equipped with an NVIDIA Jetson Xavier NX chip is established and the newly developed algorithm is tested on it.According to the results,the speed of the improved YOLOv4-Tiny algorithm in detecting on-site dress code compliance datasets is 17.25 FPS,and the mean average precision(mAP)is increased from 70.89%to 85.03%.

Random Forests Algorithm Based Duplicate Detection in On-Site Programming Big Data Environment

On-site programming big data refers to the massive data generated in the process of software development with the characteristics of real-time,complexity and high-difficulty for processing.Therefore,data cleaning is essential for on-site programming big data.Duplicate data detection is an important step in data cleaning,which can save storage resources and enhance data consistency.Due to the insufficiency in traditional Sorted Neighborhood Method(SNM)and the difficulty of high-dimensional data detection,an optimized algorithm based on random forests with the dynamic and adaptive window size is proposed.The efficiency of the algorithm can be elevated by improving the method of the key-selection,reducing dimension of data set and using an adaptive variable size sliding window.Experimental results show that the improved SNM algorithm exhibits better performance and achieve higher accuracy.

New Explorations of Laboratory and On-site Testing of Broadband Seismographs in China

According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.

Increased Effluent Dosage Effects on On-Site Wastewater Treatment Systems of Differing Architecture Type

Approximately 20% of homes nationwide use an on-site treatment system as a form of household wastewater management. However, approximately 10% to 20% of on-site treatment systems malfunction each year, many of which have either failed or exceeded the soil’s long-term acceptance rate (LTAR), causing environmental and human health risks. The objective of this field study was to evaluate the effects of soil condition (e.g., wet and dry) and product architecture type [i.e., chamber, gravel-less-pipe (GLP), polystyrene-aggregate, and pipe-and-aggregate] on in-product solution storage and biomat thickness in a profile-limited soil in northwest Arkansas under increased loading rates and to estimate the LTAR for each product. During Phase I of this study (March 13 to October 4, 2013), effluent loading rates were approximately doubled, while rates were approximately quadrupled during Phase II (October 8, 2013 to May 29, 2014), from the maximum allowable loading rate for each product. The pipe-and-tire-chip, 46-cm-wide trench pipe-and-gravel, and the 25-cm diameter GLP products had the greatest (p < 0.001), while the 31-cm-width and the 5.4-m-long chambers had the lowest (p < 0.001) in-product solution storage during wet-soil conditions of Phase I monitoring. The 25-cm diameter GLP product had the greatest (p < 0.001), while the 61-cm-width, 5.4-m-long chamber had the lowest (p < 0.001) in-product solution storage during Phase II. Results of this study indicate that some alternative products may be able to effectively handle effluent loading rates in excess of those currently allowed by the State of Arkansas. Further research will be required to confirm these interpretations.

On-Site Calibration Method of Dosimeter Based on X-Ray Source

The real-time monitoring of environmental radiation dose for nuclear fa-cilities is an important part of safety, in order to guarantee the accuracy of the monitoring results regular calibration is necessary. Around nuclear facilities there are so many environmental dosimeters installed dispers-edly, because of its huge quantity, widely distributed, and in real-time monitoring state;it will cost lots of manpower and finance if it were tak-en to calibrate on standard laboratory;what’s more it will make the en-vironment out of control. To solve the problem of the measurement ac-curacy of the stationary gamma radiation dosimeter, an on-site calibra-tion method is proposed. The radioactive source is X-ray spectrum, and the dose reference instrument which has been calibrated by the national standard laboratory is a high pressure ionization. On-site calibration is divided into two parts;firstly the energy response experiment of dosim-eter for high and low energy is done in the laboratory, and the energy response curve is obtained combining with Monte Carlo simulation;sec-ondly experiment is carried out in the field of the measuring dosimeter, and the substitution method to calibrate the dosimeter is used;finally the calibration coefficient is gotten through energy curve correction. In order to verify the accuracy of on-site calibration method, the calibrated dosimeter is test in the standard laboratory and the error is 3.4%. The re-sult shows that the on-site calibration method using X-ray is feasible, and it can improves the accuracy of the measurement results of the stationary &gamma;-ray instrument;what’s more important is that it has great reference value for the radiation safety management and radiation environment evaluation.

Dynamic Modeling Framework of Sediment Trapped by Check-Dam Networks:A Case Study of a Typical Watershed on the Chinese Loess Plateau

Check-dam construction is an effective and widely used method for sediment trapping in the Yellow River Basin and other places over the world that are prone to severe soil erosion.Quantitative estimations of the dynamic sediment trapped by check dams are necessary for evaluating the effects of check dams and planning the construction of new ones.In this study,we propose a new framework,named soil and water assessment tool(SWAT)–dynamic check dam(DCDam),for modeling the sediment trapped by check dams dynamically,by integrating the widely utilized SWAT model and a newly developed module called DCDam.We then applied this framework to a typical loess watershed,the Yan River Basin,to assess the time-varying effects of check-dam networks over the past 60 years(1957–2016).The DCDam module generated a specific check-dam network to conceptualize the complex connections at each time step(monthly).In addition,the streamflow and sediment load simulated by using the SWAT model were employed to force the sediment routing in the check-dam network.The evaluation results revealed that the SWAT-DCDam framework performed satisfactorily,with an overestimation of 11.50%,in simulating sediment trapped by check dams,when compared with a field survey of the accumulated sediment deposition.For the Yan River Basin,our results indicated that the designed structural parameters of check dams have evolved over the past 60 years,with higher dams(37.14%and 9.22%increase for large dams and medium dams,respectively)but smaller controlled areas(46.03%and 10.56%decrease for large dams and medium dams,respectively)in recent years.Sediment retained by check dams contributed to approximately 15.00%of the total sediment load reduction in the Yan River during 1970–2016.Thus,our developed framework can be a promising tool for evaluating check-dam effects,and this study can provide valuable information and support to decision-making for soil and water conservation and check-dam planning and management.

Flood risk assessment of check dams in the Wangmaogou watershed on the Loess Plateau of China

Check dams have been widely used in China’s Loess Plateau region due to their effectiveness in erosion and flood control.However,the safety and stability of the check dam decrease with the operation process,which increases the probability of dam failure during flood events and threatens local residents’ life and property.Thus,this study simulated flood process of the check dam failure in the Wangmaogou watershed in Yulin City,Shaanxi Province,China,calculated different types of inundation losses based on the flood inundation area within the watershed,and determined the number of key flood protection check dams by classifying the flood risk levels of the check dams.The results showed that 5 dams in the watershed were subject to overtopping during different rainfall return periods,which was related to their flood discharge capacity.Dam failure flood process showed a rapid growth trend followed by slow decrease,and the time of flood peak advanced with increase in the return period.After harmonization of evaluation scales,the magnitude of flood inundation losses can be ranked as:economic losses(212.409 million yuan) > life losses(10.368 million yuan) > ecological losses(6.433 million yuan).The risk value for both individual dams and the whole dam system decreases as the return period increases.The number of key flood protection check dams in the Wangmaogou watershed was 2,3,3,3,4,and 5 for floods with return periods of 10,20,30,50,100,and 200 years,respectively.The results provided a theoretical basis for the safe operation and risk evaluation of check dams in the Loess Plateau Hills watershed.

Identifying Counterexamples Without Variability in Software Product Line Model Checking

Product detection based on state abstraction technologies in the software product line(SPL)is more complex when compared to a single system.This variability constitutes a new complexity,and the counterexample may be valid for some products but spurious for others.In this paper,we found that spurious products are primarily due to the failure states,which correspond to the spurious counterexamples.The violated products correspond to the real counterexamples.Hence,identifying counterexamples is a critical problem in detecting violated products.In our approach,we obtain the violated products through the genuine counterexamples,which have no failure state,to avoid the tedious computation of identifying spurious products dealt with by the existing algorithm.This can be executed in parallel to improve the efficiency further.Experimental results showthat our approach performswell,varying with the growth of the system scale.By analyzing counterexamples in the abstract model,we observed that spurious products occur in the failure state.The approach helps in identifying whether a counterexample is spurious or genuine.The approach also helps to check whether a failure state exists in the counterexample.The performance evaluation shows that the proposed approach helps significantly in improving the efficiency of abstraction-based SPL model checking.

Check dam extraction from remote sensing images using deep learning and geospatial analysis:A case study in the Yanhe River Basin of the Loess Plateau,China

Check dams are widely used on the Loess Plateau in China to control soil and water losses,develop agricultural land,and improve watershed ecology.Detailed information on the number and spatial distribution of check dams is critical for quantitatively evaluating hydrological and ecological effects and planning the construction of new dams.Thus,this study developed a check dam detection framework for broad areas from high-resolution remote sensing images using an ensemble approach of deep learning and geospatial analysis.First,we made a sample dataset of check dams using GaoFen-2(GF-2)and Google Earth images.Next,we evaluated five popular deep-learning-based object detectors,including Faster R-CNN,You Only Look Once(version 3)(YOLOv3),Cascade R-CNN,YOLOX,and VarifocalNet(VFNet),to identify the best one for check dam detection.Finally,we analyzed the location characteristics of the check dams and used geographical constraints to optimize the detection results.Precision,recall,average precision at intersection over union(IoU)threshold of 0.50(AP_(50)),IoU threshold of 0.75(AP_(75)),and average value for 10 IoU thresholds ranging from 0.50-0.95 with a 0.05 step(AP_(50-95)),and inference time were used to evaluate model performance.All the five deep learning networks could identify check dams quickly and accurately,with AP_(50-95),AP_(50),and AP_(75)values higher than 60.0%,90.0%,and 70.0%,respectively,except for YOLOv3.The VFNet had the best performance,followed by YOLOX.The proposed framework was tested in the Yanhe River Basin and yielded promising results,with a recall rate of 87.0%for 521 check dams.Furthermore,the geographic analysis deleted about 50%of the false detection boxes,increasing the identification accuracy of check dams from 78.6%to 87.6%.Simultaneously,this framework recognized 568 recently constructed check dams and small check dams not recorded in the known check dam survey datasets.The extraction results will support efficient watershed management and guide future studies on soil erosion in the Loess Plateau.

Sediment sources and their impacts on a check dam-controlled watershed, Loess Plateau, China

Soil erosion is a major issue in Loess Plateau,China,and quantitative analyses of sediment sources are crucial for soil erosion control.In this study,a combination of flood couplet analysis and composite fingerprint identification was used for historical reconstructions of soil erosion in sediment source areas in Loess Plateau.Each flood couplet was constructed based on sediment 137Cs activity,and past soil erosion was calculated using soil bulk density and storage capacity curves.The contribution rates of the sediment sources were calculated using the fingerprinting method,and the amount of erosion in the sediment source areas was estimated.The best fingerprint combination(Cr,Ni,V,and TOC)enabled a 97.2%recognition of sediment sources from 29 flood events(1956–1990)in the Loess Plateau.The contribution rates of gullies,farmland,grassland,and shrubland were 44.89%,26.38%,10.49%,and 18.24%,respectively.These four land use types contributed 1,227,751,512,and 279 tons of sediments,respectively.Re-vegetation decreased soil erosion(1966–1983),whereas deforestation increased soil erosion(1956–1965 and 1984–1990).Rational soil and water conservation measures on slopes and check dam construction in gullies are therefore suggested to mitigate erosion.

Fast clogging problem of open check dams and a new type suggestion:curved footed type open check dam

Driftwood is one of the important physical components in mountainous rivers which causes severe hazards due to the clogging of bridges,culverts,and narrow sections during floods.Therefore,the understanding of driftwood dynamics and mitigation measures are crucial for managing wood in rivers.Open check dams are the most commonly used engineering measure for preventing driftwood from reaching downstream areas.Nevertheless,these open check dams frequently lose their sediment transport function when they are blocked by sediment and driftwood,especially during major flood events.This paper proposes a new type of open check dam for preventing from clogging.Thus,flume experiments were conducted to examine the influence of different types of open check dams on the characteristics of driftwood deposition.For the model with wood length(LWD)=16.5 cm,wood diameter(D)=15 mm,and wood number(N)=172,the highest trapping efficiency was observed with 90.1%and 87.2%retention rates for the classical debris flow breaker and curved footed open check dams,respectively.Laboratory tests showed that through this proposed design,woody debris blockage in a very short time was prevented from the accumulation of woods beside the dam.In addition to this,most of the sediment passed through the check dam and most of the driftwood got trapped.It can be briefly stated that the geometrical design of the structure plays an important role and can be chosen carefully to optimize trapping efficiency.By designing this type of open check dams in mountain river basins,it may provide a better understanding of the driftwood accumulation and basis for the optimal design of these structures.Further development of the solution proposed in this work can pave the way for designing different types of open check dams for effective flood management.

Review of Software Model-Checking Techniques for Dealing with Error Detection in Program Codes

Debugging software code has been a challenge for software developers since the early days of computer programming. A simple need, because the world is run by software. So perhaps the biggest engineering challenge is finding ways to make software more reliable. This review provides an overview of techniques developed over time in the field of software model checking to solve the problem of detecting errors in program code. In addition, the challenges posed by this technology are discussed and ways to mitigate them in future research and applications are proposed. A comprehensive examination of the various model verification methods used to detect program code errors is intended to lay the foundation for future research in this area.

Study on Problems and Countermeasures in the Management of Spot Check of Chinese Herbal Pieces

Objective To provide suggestions for optimizing the sampling management of Chinese herbal pieces and improving the quality Chinese herbal pieces.Methods Logical reasoning method was used to analyze the problems in the drug spot check of Chinese herbal pieces based on the data of drug quality published by the National Medical Products Administration from 2013 to 2021,combined with the current provisions and implementation of drug spot check management in China.Results and Conclusion At present,there are some problems in the drug spot check of Chinese herbal pieces,such as fewer varieties of Chinese herbal pieces in the national drug spot check,the unreasonable sampling links,the insufficient territorial management in some areas,and error-prone sample extraction.It is suggested that the actual quantity and overall quality of Chinese herbal pieces should be fully considered in the formulation of sampling plan.Besides,the variety coverage of drug spot check of traditional Chinese medicine should be strengthened.In the planning,the sampling links shall be made as a whole,and the credit rating and distribution of drug production,operation and use units within the jurisdiction shall be fully combined.While strengthening the sampling management at the production end in areas with concentrated industries,regional special drug spot check should be carried out or the administrative forces at the provincial,municipal and county levels should be fully mobilized to avoid local protection.In the assigned tasks,the name of Chinese herbal pieces should be accurately expressed to avoid the sampling errors of Chinese herbal pieces with the same original medicinal materials and different processing methods.

基于BIM和知识图谱的自动审查方法

本文在设计合规性审查中引入知识图谱技术,提出以规范知识图谱为核心的自动审查系统。通过对中国规范及结构设计规则(以下统称为规范)分析,提出面向规范最小语义单元的规范知识图谱元结构,建立了反映建筑规范复杂语义逻辑元结构的链接规则,进而以元结构为基元提出了规范知识图谱的构建方法。通过从图谱中获取数据,驱动代码生成模块自动生成用于信息检索和审查的可执行规范审查代码,可实现BIM模型的自动审查。

基于Q-checker的直升机MBD模型质量自动检查应用研究

从模型质量控制重要性入手,在现有MBD模型质量控制手段的基础上,对模型质量检查工具Q-check与审签系统相结合的质量控制应用方式进行研究。加强模型质量把控,严禁未通过模型质量检查、不符合模型质量标准的MBD模型通过审签系统审签,以达到提高生产效率的目的。