A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Construction and Development of the National Experimental Teaching Demonstration Center for Drama and Film and Television Disciplines

Taking the National Experimental Teaching Demonstration Center of the Central Academy of Drama as an example,this article explores the construction and development of the National Experimental Teaching Demonstration Center for the practical teaching of drama and film and television disciplines.The article first introduces the background and importance of the demonstration center and then analyzes in depth the construction content and achievements of the demonstration center from four aspects:practical teaching philosophy,practical teaching system,practical teaching resources,and practical teaching effects.Finally,the article summarizes the experience and shortcomings of the demonstration center and proposes future development directions and suggestions.

Cheesy material on macroscopic on-site evaluation after endoscopic ultrasound-guided fine-needle biopsy:Don't miss the tuberculosis

Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)is an excellent investigation to diagnose pancreatic lesions and has shown high accuracy for its use in pathologic diagnosis.Recently,macroscopic on-site evaluation(MOSE)performed by an endoscopist was introduced as an alternative to rapid on-site cytologic evaluation to increase the diagnostic yield of EUS-FNB.The MOSE of the biopsy can estimate the adequacy of the sample directly by the macroscopic evaluation of the core tissue obtained from EUS-FNB.Isolated pancreatic tuberculosis is extremely rare and difficult to diagnose because of its non-specific signs and symptoms.Therefore,this challenging diagnosis is based on endoscopy,imaging,and the bacteriological and histological examination of tissue biopsies.This uncommon presentation of tuberculosis can be revealed as pancreatic mass mimicking cancer.EUS-FNB can be very useful in providing a valuable histopathological diagnosis.A calcified lesion with a cheesy core in MOSE must be suggestive of tuberculosis,leading to the request of the GeneXpert,which can detect Mycobacterium tuberculosis deoxyribonucleic acid and resistance to rifampicin.A decent diagnostic strategy is crucial to prevent unnecessary surgical resection and to supply conservative management with antitubercular therapy.

Rural sustainable development:A case study of the Zaozhuang Innovation Demonstration Zone in China

Sustainable development is the central theme of modern global development.With the arrival of the urban era,the vulnerability and instability of rural areas have significantly increased,and rural sustainable development faces serious challenges.To address these issues,the study took the Zaozhuang Innovation Demonstration Zone in China under the National Sustainable Development Agenda as a case,combined with economic,social and land use data during 2016-2020,and applied Granger causality test method to explore the theoretical and practical pathways of“innovation-driven rural sustainable development”.The results showed that rural sustainable development and economic sustainability displayed a trend of synergistic change,with“explosive”growth from 2018 to 2020.The social sustainability steadily increased from 2016 to 2020.Ecological and spatial sustainability continuously declined during the study period.Moreover,the rural innovation capacity of the Zaozhuang Innovation Demonstration Zone displayed rapid growth during 2016-2020.Although the rural innovation capacity of the Zaozhuang Innovation Demonstration Zone has rapidly improved,it has a weak driving effect on rural sustainable development and economic sustainability.There are two primary challenges that must be overcome to ensure the rural sustainable development of the Zaozhuang Innovation Demonstration Zone.The first challenge is the imbalance among the multi-dimensional relationships in the process of rural sustainable development,and the second challenge is the weakening of rural innovation capacity to drive rural sustainable development.To overcome these challenges,this study proposed a systematic pathway for rural sustainable development in the Zaozhuang Innovation Demonstration Zone from multidimensions,such as policy actions,technologies,projects,and institutional guarantees,and formed a universal and representative“Zaozhuang model”.This study expands the theoretical foundation of rural sustainable development and provides theoretical and practical support for innovation-driven rural sustainable development.

Research on the Strategy of Promoting Daqing to Create a National Public Employment and Entrepreneurship Service Demonstration City

Based on the research on the basic status quo of the construction of public employment and entrepreneurship service system in Daqing, this paper analyzes the problems existing in Daqing’s establishment of a national public employment and entrepreneurship service demonstration city, and gives countermeasures and suggestions from three levels: optimizing the public employment and entrepreneurship service system, innovating the public employment and entrepreneurship service mode, and improving the entrepreneurship driven employment support system.

Evaluation of Women’s Contribution in Agroforestry Demonstration through Moringa Species Introduction in Western Oromia,Ethiopia

Agroforestry technologies are very keen practices in most small-scale farming systems where gender issues are not well considered in most developing countries.Moringa is a tropical plant that consists of 13 species,while five of them are found in Ethiopia.This study aimed to evaluate the potential and contribution of women in agroforestry demonstrations where Moringa oleifera and Moringa stenopetala are introduced in farmers’fields.Selection of the best performing moringa species and preferred by farmers for further scaling up was also another objective.The activity was conducted in five districts of west and east Wallagga zones.A total of 10 FREG comprising 150 farmers were established.The two moringa species were planted on 100 farmers’fields where 50 women and 50 men were purposively selected and given responsibilities of moringa demonstration.Leaflets and practical training on moringa production,importance and utilization were prepared and given to all concerned bodies.84%of women and only 32%of men effectively demonstrated the moringa species.A total of 200 farmers were interviewed to evaluate and select the best from the two species depending on their growth performance,fresh leaf taste and odor,and survival rate.Based on the above criteria,143(71.5%)farmers preferred Moringa oleifera.In general,the two moringa species were performed well in most places,and its utilization started at household level.Finally,the authors recommend that women are the potential for demonstrating agroforestry technologies,and Moringa oleifera is a more preferred species in western Oromia.

Plan and Practice of Ecological and Leisure Agricultural Demonstration Park ——A case of Xiangtian Village in Jin'an County

With Xiangtian Village, Jin＇an County as an example, development objec- tive and functional orientation of ecological and leisure agricultural demonstration park were described in detail, based on which a characteristic and promising plan of the park was proposed.

Planning of Modern Agricultural Demonstration Zone from the Perspective of Industrial Convergence——Also Study on the Expansion of Its Tourist Functions

Features of agritourism emerging in the development of modern agriculture were analyzed, it was stressed that industrial convergence was a basic feature of modern agriculture, and modern agriculture was the integration of agriculture, science and technology, integration of agriculture, industry and trade, and integration of rural and urban areas. Agritourism was believed as the optimal carrier of expanding functions of modern agriculture combining agriculture, ecology and tourism industry. From such perspectives, causes for the loss of tourist functions in the development of agricultural demonstration zone were identified as 5 aspects, specifically including various planners from diverse fields; no any technical standard for the planning, or systematic planning theory and technique; many individual planning cases with poor applicability; no innovative idea brought into the tourism project planning; the loss of cultures in new tourist products. After proposing key points in the expansion planning of tourist functions, this research pointed out 4 countermeasures, i.e. (1) design concepts should be changed; (2) planning functions should be reoriented to match service facility planning with laws of tourism development and properly design tourist projects; (3) landscaping planning should be focused on; and (4) eco-environment protection and brand building should be enhanced.

Research of Ecological Restoration of Jari Agricultural Demonstration Site in Ethiopia

In order to resolve the issue of soil erosion in East African plateau, a micro-landscape irrigation region was established in Ethiopia plateau to research status quo of agricultural demonstration site in Jari in Ethiopia and to analyze the relation between structure and function of inner elements in different landscapes of demonstration site. Furthermore, in accordance with ecology, silviculture, agriculture and economics, the ecological landscapes were classified as per landscape functions; lands returning from farming were classified and re-used; ecosystems of grassland, river, agriculture and courtyard were researched; the feasibility of ecological restoration and sustainable development in demonstration site was demonstrated; the model for rational irrigation and water conservation was proposed. The research guarantees sustainable development of agriculture and animal husbandry and provides references for undeveloped countries with similar problems.

Problem and Countermeasure Analysis of Popularization Mode for Rural Residential Demonstration Project

Popularization mode of government-dominated rural residential demonstration projects and its features were introduced.It was proposed that such a popularization mode still remained at the primary development stage and was still suffering from many problems such as guiding role of government not fully played,lack of assessment standards,unitary funding channels,ineffective popularization effects and unpractical management regulations.In view of these problems,popularization strategies for rural residential demonstration projects were given from the perspective of establishing authorized coordination and supervision agencies,improving incentive policies of demonstration projects and also regulation system for these projects,expanding funding channels,and building information platforms.The study was to provide practical references for the future development of rural residential demonstration projects.

Selection of Tree and Shrub Species for Ecological Recovery in Jari Demonstration Plot in Ethiopia

Environmental degradation and deforestation in Jari Demonstration Plot needed forestation and reforestation,and the plant species selection was a key issue in the ecological recovery.Ecological information was fully considered regarding each species in various agro-climatic zones,altitude ranges,specific niches in different landscapes,soil preference,drought resistance and certain other important ecological factors.The species selection model represents an idea of ecological recovery and soil erosion control in the demonstration plot.

Public Speaking的技巧与策略(9)——如何进行Demonstration Presentation

你也许经常听到有人问你“做给我看看好吗?”我们几乎都是在演示过程中学会做每件事的。有时这种演示是正式的,比如在工厂或某种技术培训时。在其它情况下的演示则为非正式的。在演示过程中,我们通过观察和提问来学习,最后自己动手做某件事。教别人做事的过程就是一个演示过程,演示性演讲用途广泛,因此,对我们每个人来说都很重要。掌握了演示性演讲的技巧。

Potential and Suitability Evaluation of CO_2 Geological Storage in Major Sedimentary Basins of China,and the Demonstration Project in Ordos Basin

From 2010 to 2012, the China Geological Survey Center for Hydrogeology and Environmental Geology Survey （CHEGS） carried out the project ＂Potential evaluation and demonstration project of CO2 Geological Storage in China＂. During this project, we developed an evaluation index system and technical methods for the potential and suitability of CO2 geological storage based on China＇s geological conditions, and evaluated the potential and suitability of the primary basins for COz geological storage, in order to draw a series of regional scale maps （at a scale of 1：5000000） and develop an atlas of the main sedimentary basins in China. By using these tools, we delineated many potential targets for CO2 storage. We also built techniques and methods for site selection and the exploration and assessment of CO2 geological storage in deep saline aquifers. Furthermore, through cooperation with the China Shenhua Coal to Liquid and Chemical Co., Ltd., we successfully constructed the first coal-based demonstration project for CO2 geological storage in deep saline aquifers in the Yijinhuoluo Banner of Ordos in the Inner Mongolia Autonomous Region, which brought about the basic preliminary theories, techniques, and methods of geological CO2 storage in deep saline aquifers under China＇s geological conditions.

Rapid on-site evaluation of endoscopic-ultrasound-guided fine-needle aspiration diagnosis of pancreatic masses

Endoscopic ultrasound (EUS) has become an essential tool for the study of pancreatic diseases. Specifically, EUS plays a pivotal role evaluating patients with a known or suspected pancreatic mass. In this setting, differential diagnosis remains a clinical challenge. EUS-guided fine-needle aspiration (FNA) and fine-needle biopsy (FNB) have been proven to be safe and useful tools in this setting. EUS-guided FNA and FNB, by obtaining cytological and/or histological samples, are able to diagnose pancreatic lesions with high sensitivity and specificity. In this context, several methodological features, trying to increase the diagnostic yield of EUS-guided FNA and FNB, have been evaluated. In this review, we focus on the role of rapid on-site evaluation (ROSE). From data reported in the literature, ROSE may increase diagnostic yield of EUS-FNA specimens by 10%-30%, and thus, diagnostic accuracy. However, we should point out that many recent studies have reported adequacy rates of > 90% without ROSE, indicating that, perhaps, at high-volume centers, ROSE may not be indispensable to achieve excellent results. The use of ROSE can be considered important during the learning curve of EUS-FNA, and also in hospital with diagnostic accuracy rates < 90%.

Poverty alleviation through e-commerce:Village involvement and demonstration policies in rural China

The diffusion of e-commerce has played a significant role in recent rural economic development in China.E-commerce is also considered as an efficient channel to alleviate poverty in rural China.Voluminous studies have investigated the contribution of e-commerce to agricultural development,yet it is lacking empirical evidence as to the effects of e-commerce on rural poverty alleviation.Since the year of 2014,in order to develop rural e-commerce,Chinese government launched the National Rural E-commerce Comprehensive Demonstration Project.This gradual involvement policy offered a natural experiment for evaluation of e-commerce.Based on village-level survey data from rural China and Heckit method,our study finds that rural e-commerce has a significantly positive effect on rural income.Moreover,the effect is inverted U-shaped for the relative-poverty villages.The estimation of the propensity scores matching model confirms that the results are robust.The following policy recommendations are proposed:(1)policy support to rural e-commerce should prioritize the povertystricken villages.By doing so,the marginal income effects of e-commerce will be maximized.(2)Investment in internet infrastructure and establishment of human resources for e-commerce in rural areas will have spillover effects,increasing rural income through the"digital dividend".

Demonstration of a Sub-Sampling Phase Lock Loop Based Microwave Source for Reducing Dick Effect in Atomic Clocks

We demonstrate a simple scheme of 6.835 GHz microwave source based on the sub-sampling phase lock loop(PLL). A dielectric resonant oscillator of 6.8 GHz is directly phase locked to an ultra-low phase noise 100 MHz oven controlled crystal oscillator(OCXO) utilizing the sub-sampling PLL. Then the 6.8 GHz is mixed with 35 MHz from an direct digital synthesizer(DDS) which is also referenced to the 100 MHZ OCXO to generate the final6.835 GHz signal. Benefiting from the sub-sampling PLL, the processes of frequency multiplication, which are usually necessary in the development of a microwave source, are greatly simplified. The architecture of the microwave source is pretty simple. Correspondingly, its power consumption and cost are low. The absolute phase noises of the 6.835 GHz output signal are-47 d Bc/Hz,-77 dBc/Hz,-104 dBc/Hz and-121 dBc/Hz at1 Hz, 10 Hz, 100 Hz and 1 kHz offset frequencies, respectively. The frequency stability limited by the phase noise through the Dick effect is theoretically estimated to be better than 5.0 × 10^-14τ^1/2 when it is used as the local oscillator of the Rb atomic clocks. This low phase noise microwave source can also be used in other experiments of precision measurement physics.

Vehicle fuel from biogas with carbon membranes; a comparison between simulation predictions and actual field demonstration

The energy contents of biogas could be significantly enhanced by upgrading it to vehicle fuel quality.A pilot-scale separation plant based on carbon hollow fiber membranes for upgrading biogas to vehicle fuel quality was constructed and operated at the biogas plant,Gl?r IKS,Lillehammer Norway.Vehicle fuel quality according to Swedish legislation was successfully achieved in a single stage separation process.The raw biogas from anaerobic digestion of food waste contained 64±3 mol%CH_4,30–35 mol%CO_2 and less than one percent of N_2 and a minor amount of other impurities.The raw biogas was available at 1.03 bar with a maximum flow rate of 60 Nm^3h^(à1).Pre-treatment of biogas was performed to remove bulk H_2O and H_2S contents up to the required limits in the vehicle fuel before entering to membrane system.The membrane separation plant was designed to process 60 Nm^3h^(à1)of raw biogas at pressure up to 21 bar.The initial tests were,however,performed for the feed flow rate of 10 Nm^3h^(à1)at 21 bar.The successful operation of the pilot plant separation was continuously run for 192 h(8days).The CH_4 purity of 96%and maximum CH_4 recovery of 98%was reached in a short-term test of 5 h.The permeate stream contained over20 mol%CH_4which could be used for the heating application.Aspen Hysys~?was integrated with Chem Brane(in-house developed membrane model)to run the simulations for estimation of membrane area and energy requirement of the pilot plant.Cost estimation was performed based on simulation data and later compared with actual field results.

Comprehensive Evaluation on Demonstration Farmers' Specialized Cooperatives on the Basis of AHP Model

On the basis of AHP method,this paper establishes comprehensive evaluation model of demonstration farmers' specialized cooperatives,uses the Delphi method and AHP software to assign scientific and objective weight to 7 criteria in criteria layer and 27 indices in index layer,and evaluate the overall level of 153 farmers' specialized cooperatives whose main business is fruits and vegetables in Region R.The results show that the overall operation level of farmers' specialized cooperatives is common;democratic and financial management is open;the information-based level and social sensation are not insufficient;the marking results of questionnaire is basically scientific and objective.This paper discusses the new way to evaluate operation level of farmers' specialized cooperatives,and puts forward relevant countermeasures and suggestions as follows:promote the information-based level of demonstration cooperatives;strengthen the service capacity of demonstration cooperatives;use means of science and technology to promote product quality;amplify social sensation of farmers' specialized cooperatives.

Constructing demonstration zones to promote the implementation of Sustainable Development Goals

After the United Nations Agenda 2030 was officially launched in September 2015,governments around the world have taken actions to implement the ambitious plan.This paper summarizes the actions and policies taken in China for promoting the implementation of the UN’2030 Agenda for Sustainable Development,with emphasis on promoting the development of demonstration zones for delivery of the Sustainable Development Goals(SDGs).The Chinese government has taken two types of measures.One is to formulate a series of national plans and strategies and the other is to construct pilot and demonstration zones.Two approaches were presented to optimize the selection criteria for demonstration zones:a problem-based approach;and a major function-based approach.Both provided priority regions and major themes for selection and construction of demonstration zones.Suggestions for promoting the development of demonstration zones include:(1)building a monitoring system for the implementation of SDGs in each demonstration zone and establishing an open online platform to track the progress;(2)forming an effective stakeholder participation mechanism.The experiences in constructing innovation-driven demonstration zones are insightful for other countries to take practical actions in the delivery of SDGs.

Planning and Ecological Greening of Agricultural Sci-Tech Ecological Demonstration Park in Suning County,Hebei Province

Taking the control detailed planning of Suning Agricultural Sci-Tech Ecological Demonstration Park in Hebei Province for example,general methods for the planning of modern agricultural sci-tech parks were introduced from the perspective of general situation of the planning area,technical routes,guiding concepts and planning principles,ecological greening and plant confi guration of the parks was discussed.