A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Research Progress of Atrial Fibrillation Detection Technology

Atrial fibrillation (AF) is the most common chronic arrhythmia in clinical practice, which can cause high disability and mortality with the progress of the disease. Many studies at home and abroad have shown that the incidence of atrial fibrillation gradually increases with age. Clinically, the onset of most AF patients is insidious, which is difficult to capture by routine electrocardiogram, and there is some difficulty in the diagnosis. In order to make the early diagnosis of atrial fibrillation more efficient and accurate, this paper reviews the current status and research progress of detection technology for atrial fibrillation at home and abroad, in order to provide a scientific basis for the early diagnosis of atrial fibrillation.

Analysis of Detection and Monitoring Technology in the Construction and Maintenance of Large Bridges

Currently,there is significant attention placed on the construction,management,and maintenance of large service bridges.Within the realm of bridge maintenance management,the utilization of detection and monitoring technology is indispensable.By employing these technologies,we can effectively identify any structural defects within the bridge,promptly uncover unknown risks,proactively establish maintenance strategies,and prevent the rapid deterioration of bridge conditions.This article aims to explore the advantages of applying bridge monitoring and testing technology and to discuss various methods for implementing detection and monitoring technology throughout the construction,management,and maintenance phases of large bridges.Ultimately,this will contribute to ensuring the safe operation of large bridges.

Acoustic Non-Destructive Testing Technology in Concrete Bridge Inspection and Pile Foundation Detection

This article takes the actual construction project of a certain concrete bridge project as an example to analyze the application of acoustic non-destructive testing technology in its detection.It includes an overview of a certain bridge construction project studied and acoustic non-destructive testing technology and the application of acoustic non-destructive testing technology in actual testing.This analysis hopes to provide some guidelines for acoustic non-destructive testing of modern concrete bridge projects.

Applications of Ultrasonic Detection Technology in Bridge Concrete Pile Foundation Detection

In this paper,the application strategy of ultrasonic detection technology in the detection of concrete foundation piles is analyzed using a construction project as an example.It includes a basic overview of the project,an overview of ultrasonic testing technology in bridge concrete pile foundation testing,and an analysis of its practical application in the concrete pile foundation testing of this project.The objective of this analysis is to provide some reference for the application of ultrasonic testing technology and the improvement of the quality of bridge concrete pile foundation testing.

Research on Infrared Image Fusion Technology Based on Road Crack Detection

This study aimed to propose road crack detection method based on infrared image fusion technology.By analyzing the characteristics of road crack images,this method uses a variety of infrared image fusion methods to process different types of images.The use of this method allows the detection of road cracks,which not only reduces the professional requirements for inspectors,but also improves the accuracy of road crack detection.Based on infrared image processing technology,on the basis of in-depth analysis of infrared image features,a road crack detection method is proposed,which can accurately identify the road crack location,direction,length,and other characteristic information.Experiments showed that this method has a good effect,and can meet the requirement of road crack detection.

Discussion on Test and Detection Technology of Highway Asphalt Pavement

The road traffic industry in China has been developing rapidly in recent years.Asphalt has become the main material in road construction because of its seamless connection and short construction period,and it has an important impact on the overall quality of highway construction.Therefore,it is necessary to conduct scientific and reasonable tests and detections for highway asphalt pavement.This paper presents a discussion on the test and detection technology of highway asphalt pavement for future reference.

Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1

Human bocavirus(HBoV)1 is considered an important pathogen that mainly affects infants aged 6–24 months,but preventing viral transmission in resource-limited regions through rapid and affordable on-site diagnosis of individuals with early infection of HBoV1 remains somewhat challenging.Herein,we present a novel faster,lower cost,reliable method for the detection of HBoV1,which integrates a recombinase polymerase amplification(RPA)assay with the CRISPR/Cas12a system,designated the RPA-Cas12a-fluorescence assay.The RPA-Cas12a-fluorescence system can specifically detect target gene levels as low as 0.5 copies of HBoV1 plasmid DNA per microliter within 40 min at 37℃without the need for sophisticated instruments.The method also demonstrates excellent specificity without cross-reactivity to non-target pathogens.Furthermore,the method was appraised using 28 clinical samples,and displayed high accuracy with positive and negative predictive agreement of 90.9%and 100%,respectively.Therefore,our proposed rapid and sensitive HBoV1 detection method,the RPA-Cas12a-fluorescence assay,shows promising potential for early on-site diagnosis of HBoV1 infection in the fields of public health and health care.The established RPA-Cas12a-fluorescence assay is rapid and reliable method for human bocavirus 1 detection.The RPA-Cas12a-fluorescence assay can be completed within 40 min with robust specificity and sensitivity of 0.5 copies/μl.

Review of advanced road materials, structures, equipment, and detection technologies

As a vital and integral component of transportation infrastructure,pavement has a direct and tangible impact on socio-economic sustainability.In recent years,an influx of groundbreaking and state-of-the-art materials,structures,equipment,and detection technologies related to road engineering have continually and progressively emerged,reshaping the landscape of pavement systems.There is a pressing and growing need for a timely summarization of the current research status and a clear identification of future research directions in these advanced and evolving technologies.Therefore,Journal of Road Engineering has undertaken the significant initiative of introducing a comprehensive review paper with the overarching theme of“advanced road materials,structures,equipment,and detection technologies”.This extensive and insightful review meticulously gathers and synthesizes research findings from 39 distinguished scholars,all of whom are affiliated with 19 renowned universities or research institutions specializing in the diverse and multidimensional field of highway engineering.It covers the current state and anticipates future development directions in the four major and interconnected domains of road engineering:advanced road materials,advanced road structures and performance evaluation,advanced road construction equipment and technology,and advanced road detection and assessment technologies.

Analysis of Bridge Inspection Technology for Highway Reconstruction and Expansion

This article focuses on bridge testing technologies in highway construction and expansion projects.It provides an overview of the inspection process for bridges in highway reconstruction and expansion projects,the main inspections,and strategies for improving the quality of inspections.Relevant units should conduct bridge inspections using appearance inspections,special inspections,and bearing capacity assessments in highway reconstruction and expansion projects.To effectively improve testing quality,relevant units should also focus on establishing and improving testing standards,strengthening material testing,and improving the overall quality of the testing personnel.This research aims to enhance the quality of highway reconstruction and expansion projects by supporting the sensible application of bridge inspection technology.

Review on the application of upconversion nanomaterials in heavy metal detection

As a widespread element,heavy metals have a significant impact on human health and threaten human health.It is of great significance to develop analytical technologies that can detect heavy metal ions quickly and accurately.In comparison to conventional fluorescent materials such as organic dyes,quantum dot(QD)labels,and carbon quantum dots(CD),fluorescence detection technology utilizing lanthanide(Ln)ion-doped upconversion nanoparticles(UCNPs)stands out due to its distinctive attributes.These include a notably reduced autofluorescence background,enhanced tissue penetration capabilities,biocompatibility with cellular tissues,and minimal photodamage inflicted on biological samples.The utilization of this technology has garnered considerable attention across multiple fields.In the domain of heavy metal detection,traditional laboratory methods necessitate costly instrumentation and a fully equipped laboratory,involving intricate sample processing procedures and protracted detection periods,as well as a demand for skilled personnel.In contrast,the implementation of this material offers rapid and cost-effective detection,significantly mitigating the technical barriers for operators.Consequently,this represents an exceptional avenue to curtail expenses and broaden the scope of detection within the analytical process.This paper reviews the research progress of UCNPs in the detection of heavy metal ions,encompassing a brief elucidation of the luminescence principle of upconversion nanomaterials and commonly used detection principles.Additionally,it provides a detailed overview of the research status of several common non-metal ions and essential heavy metals.Furthermore,it summarizes the current focal points in UCNP detection and discusses the challenges and prospects associated with it.

Plant Nutrient Solution Detection System Based on ZigBee Wireless Technology

Based on the limitations of traditional plant nutrient solution detection, a ZigBee plant nutrient solution detection system based on CC2530 was developed. This system uses CC2530 as the main control chip, DS18B20 as the temperature sensor for temperature acquisition, PH electrode sensor for PH value acquisition. The experiment shows that this wireless control system equipped with temperature and pH detection sensor collects and samples the main nutrient solution parameters through the main controller, performs wireless communication transmission and terminal communication, and realizes the intelligent detection of plant nutrient solution parameters. This technique of applying wireless sensor network technology to plant factories greatly improves the reliability and stability of the nutrient monitoring system.

The analysis of application of data mining technology in the system of intrusion detection

With the economic development and the popularity of application of electronic computer, electronic commerce has rapid development. More and more commerce and key business has been carried on the lnternet because Internet has the features of interaction, openness, sharing and so on. However, during the daily commerce, people worry about the security of the network system. So a new technology which can detect the unusual behavior in time has been invented in order to protect the security of network system. The system of intrusion detection needs a lot of new technology to protect the data of the network system. The application of data mining technology in the system of intrusion detection can provide a better assistant to the users to analyze the data and improve the accuracy of the checking system.

Random Forests Algorithm Based Duplicate Detection in On-Site Programming Big Data Environment

On-site programming big data refers to the massive data generated in the process of software development with the characteristics of real-time,complexity and high-difficulty for processing.Therefore,data cleaning is essential for on-site programming big data.Duplicate data detection is an important step in data cleaning,which can save storage resources and enhance data consistency.Due to the insufficiency in traditional Sorted Neighborhood Method(SNM)and the difficulty of high-dimensional data detection,an optimized algorithm based on random forests with the dynamic and adaptive window size is proposed.The efficiency of the algorithm can be elevated by improving the method of the key-selection,reducing dimension of data set and using an adaptive variable size sliding window.Experimental results show that the improved SNM algorithm exhibits better performance and achieve higher accuracy.

Research on Obstacle Detection Method of Urban Rail Transit Based on Multisensor Technology

With the rapid development of urban rail transit,passenger traffic is increasing,and obstacle violations are more frequent,and the safety of train operation under high-density traffic conditions is becoming more and more thought provoking.In order to monitor the train operating environment in real time,this paper first adopts multisensing technology based on machine vision and lidar,which is used to collect video images and ranging data of the track area in real time,and then it performs image preprocessing and division of regions of interest on the collected video.Then,the obstacles in the region of interest are detected to obtain the geometric characteristics and position information of the obstacles.Finally,according to the danger degree of obstacles,determine the degree of impact on the train operation,and use the signal system automatic response ormanual response mode to transmit the detection results to the corresponding train,so as to control the train operation.Through simulation analysis and experimental verification,the detection accuracy and control performance of the detection method are confirmed,which provides safety guarantee for the train operation.

Intrusion Detection System with Remote Signalling for Vehicles Using an Arduino Controller and Radio-Frequency Technology

Malicious activities or policy violations have been a concern for the past years. For example, many people have been victims of robbery on vehicles. A conceptual diagram of an Intrusion Detection System (IDS) [1] [2] for vehicles with remote signaling using an Arduino controller and radio-frequency technology is proposed in this paper. To address malicious activities on vehicles, two aspects are considered here, namely: notifier and detector. Firstly, an object-oriented C module that puts on and off a controller (installed inside the vehicle) and an anti-theft electronic editing that powered using an alternator and supported by a back-up battery are implemented. Secondly, a magnetic intrusion sensor, controlled by a proximity detector using radio-frequency technology, has been installed on each vehicle door. To enable IDS, a user needs to activate the monitoring system when leaving their vehicle. This is done using a remote system. In case the user does not activate the monitoring system while leaving the vehicle, a 5-meter-proximity detector will automatically lock the system and set off the monitoring system whenever the user is outside the detection zone. The detection zone is a 5-meter radius area centered at the controller. Here, monitoring consists of geolocating any intruders within the detection zone. This means, if any of the vehicle doors is opened while the system is still locked, the controller will activate the vehicle alarm for a few seconds, thereafter send an SMS notification to the owner. The system automatically unlocks as soon as the proximity detector is within the detection zone. The contribution of this paper, as compared to other similar work, is to reinforce the electronic implementation of IDS.

Present Situation and Detection Technology of Pesticide Residues in Tobacco

This article summarizes the present situation of pesticide residue in tobacco, and introduces the extraction methods and detection tech-niques of pesticide residues commonly used in pesticide residue analysis. The advantages and disadvantages of these methods are reviewed, and the development of pesticide residue analysis technology is prospected.

Signal Processing Circuit Design of Infrared Detection System with SO2 Concentration Based on Correlation Filter Technology

Signals from infrared detector are very weak in SO2 concentration measuring system.In order to improve the sensitivity of detection,combining with filter correlation technology and infrared absorption principle,the weak signal processing circuit is designed according to correlation detection technology.Under laboratory conditions,system performance of SO2 concentration is tested,and the experimental data are analyzed and processed.Then relationship of SO2 concentration and the measuring voltage is provided to prove that the design improves measuring sensitivity of the system.

Research on Key Technology of Web Vulnerability Detection System Based on Cloud Environment

In the light of the defect of web vulnerability detection system, combined with the characteristics of high efficient and sharing in the cloud environment, a design proposal is presented based on cloud environment, which analyses the key technology of gaining the URL, task allocation and scheduling and the design of attack detection. Experiment shows its feasibility and effectiveness in this paper.

Analysis on Mathematics Teaching Goal Design Technology and Its Detection

Teaching goal design is an important link of teaching design, which has the teaching, learning and measurement function. How to establish the appropriate teaching goal and properly state to enhance its guidance and detection function are the important tasks of teaching design. This paper makes a programming and systematic analysis on the aspects of teaching goal design theory, teaching goal statement mode and technology, teaching goal statement and design and teaching goal design detection.