A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Committed to Safe Production China-Indonesia joint venture HBAP strives to ensure safe, stable operations while fulfilling social responsibility

Recently,PT Huadian Bukit Asam Power (HBAP) was awarded the"Zero Accident Award"by the Ministry of Manpower of the Republic of Indonesia.This national-level award for safe production in Indonesia serves to testify to the enterprise's achievements in safe and stable operations from June 1,2018,to December 31,2023.

Evaluation of Driver-Induced Human Errors in Smart Construction Tower Crane Operations Based on DEMATEL-ISM-MICMAC

With the advent of Industry 4.0, smart construction sites have seen significant development in China. However, accidents involving digitized tower cranes continue to be a persistent issue. Among the contributing factors, human unsafe behavior stands out as a primary cause for these incidents. This study aims to assess the human reliability of tower crane operations on smart construction sites. To proactively enhance safety measures, the research employs text mining techniques (TF-IDF-Truncated SVD-Complement NB) to identify patterns of human errors among tower crane operators. Building upon the SHEL model, the study categorizes behavioral factors affecting human reliability in the man-machine interface, leading to the establishment of the Performance Shaping Factors (PSFs) system. Furthermore, the research constructs an error impact indicator system for the intelligent construction site tower crane operator interface. Using the DEMATEL method, it analyzes the significance of various factors influencing human errors in tower crane operations. Additionally, the ISM-MICMAC method is applied to unveil the hierarchical relationships and driving-dependent connections among these influencing factors. The findings indicate that personal state, operating procedures, and physical environment directly impact human errors, while personal capability, technological environment, and one fundamental organizational management factor contribute indirectly. .

Research and Practice of the Blended Teaching Model of BOPPPS Teaching Method Under the Background of Digital Education: Taking Operations Research Course as an Example

The rapid development of digital education provides new opportunities and challenges for teaching model innovation.This study aims to explore the application of the BOPPPS(Bridge-in,Objective,Pre-assessment,Participatory learning,Post-assessment,Summary)teaching method in the development of a blended teaching model for the Operations Research course under the background of digital education.In response to the characteristics of the course and the needs of the student group,the teaching design is reconstructed with a student-centered approach,increasing practical teaching links,improving the assessment and evaluation system,and effectively implementing it in conjunction with digital educational technology.This teaching model has shown significant effectiveness in the context of digital education,providing valuable experience and insights for the innovation of the Operations Research course.

Dynamic Analysis of Pipeline Lifting Operations for Different Current Velocities and Wave Heights

Pipelines are widely used for transporting oil resources in the context of offshore oil exploitation.The pipeline stress-strength analysis is an important stage in related design and ensuing construction techniques.In this study,assuming representative work environment parameters,pipeline lifting operations are investigated numerically.More specifically,a time-domain coupled dynamic analysis method is used to conduct a hydrodynamic analysis under different current velocities and wave heights.The results show that proper operation requires the lifting points are reasonably set in combination with the length of the pipeline and the position of the lifting device on the construction ship.The impact of waves on the pipeline is limited,however lifting operations under strong wind and waves should be avoided as far as possible.

Study on the Characteristics of A New Hybrid Mooring System for DualPlatform Joint Operations

As the sustainable exploitation of marine resources develops,dual-platform joint operation has caught increasing attention.Dual-platform joint operation requires smaller relative motion between the two sub-platforms,which is normally difficult to be satisfied by the traditional mooring system.Therefore,a new hybrid mooring system is developed and studied in this article.To ensure safety during platform movements,both the number of anchor chains and the relative motion between the two sub-platforms are reduced in the new hybrid mooring system.By performing numerical simulations based on three-dimensional potential flow theory in AQWA and physical experiments,the performances of both the new hybrid and traditional mooring systems under two different wave conditions(i.e.,working wave and freak wave conditions) are systematically investigated.Regarding the new hybrid mooring system,the relative stability between the two sub-platforms of the new system is better,and the platforms can restore stability faster when affected by freak waves.

Cheesy material on macroscopic on-site evaluation after endoscopic ultrasound-guided fine-needle biopsy:Don't miss the tuberculosis

Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)is an excellent investigation to diagnose pancreatic lesions and has shown high accuracy for its use in pathologic diagnosis.Recently,macroscopic on-site evaluation(MOSE)performed by an endoscopist was introduced as an alternative to rapid on-site cytologic evaluation to increase the diagnostic yield of EUS-FNB.The MOSE of the biopsy can estimate the adequacy of the sample directly by the macroscopic evaluation of the core tissue obtained from EUS-FNB.Isolated pancreatic tuberculosis is extremely rare and difficult to diagnose because of its non-specific signs and symptoms.Therefore,this challenging diagnosis is based on endoscopy,imaging,and the bacteriological and histological examination of tissue biopsies.This uncommon presentation of tuberculosis can be revealed as pancreatic mass mimicking cancer.EUS-FNB can be very useful in providing a valuable histopathological diagnosis.A calcified lesion with a cheesy core in MOSE must be suggestive of tuberculosis,leading to the request of the GeneXpert,which can detect Mycobacterium tuberculosis deoxyribonucleic acid and resistance to rifampicin.A decent diagnostic strategy is crucial to prevent unnecessary surgical resection and to supply conservative management with antitubercular therapy.

Operations of Knapsack Sprayer and Its Impacts on Physiological Parameters of Selected Operators

The study was aimed at determining the impacts of operating Manually Operated Hand Lever Knapsack Sprayers (MOHLKS) on physiological responses of the operators as dependent on anthropometric variations and sex. Twenty eight subjects, (4 female, 24 male) Mean ± SD: Age 22.5 ± 1.92, 24.29 ± 2.2 years;Body Mass Index 24.6 ± 4.8, 21.7 ± 2.4 kg/m2 were employed in the study. Selected anthropometric parameters of weight and height were used to determine body mass index (BMI), with these are arm-reach forward, elbow to fingertip, hand length and hand width were measured to establish human variations in diversity. Subjects undertook the operation at 5 replicates each, before and after which information about operators’ body pain locations and body physiological changes of heart rates were obtained. Measured parameters were used in the determination of expended energy (EE), physiological cost (PC), oxygen intake (VO2) and aerobic power (VO2max). Alongside with these were operational parameters of stroke, pace and time taken to get the operation done and environmental factors of temperature and relative humidity. The results revealed on the average that the BMI (24.61 ± 4.78 kg/m2) in female operators was higher, this corresponded to PC and VO2, while the VO2max (34.83 ± 3.30 ml/min/kg) in males is higher. More EE was obtained in female subjects (3.53 ± 3.76 kCal/min) as compared to male subjects (3.42 ± 7.48 kCal/min). The main effects plot of operational factors on EE displayed the stroke made by the subjects during spraying operation as parameter with largest effect on EE. Regression equation for EE and PCI is given as PCI = 1.97 + 25.2 EE, while the P-value at α = 0.05 is 0.000 and R2 = 98.8%. Post operational body pain showed that 19 out of 28 subjects incurred at least one type of body pain, with shoulder pain as most frequent. The results of the study suggest that early incidence of fatigue may occur in female operators as compared to the males, and in addition, cumulative trauma at shoulder, back, and upper and lower arm may result over time. Hence, it is recommended that the tank volume should be reduced and the straps for the shoulders should be supported with additional cushion.

Fractal Fractional Order Operators in Computational Techniques for Mathematical Models in Epidemiology

New fractional operators, the COVID-19 model has been studied in this paper. By using different numericaltechniques and the time fractional parameters, the mechanical characteristics of the fractional order model areidentified. The uniqueness and existence have been established. Themodel’sUlam-Hyers stability analysis has beenfound. In order to justify the theoretical results, numerical simulations are carried out for the presented methodin the range of fractional order to show the implications of fractional and fractal orders.We applied very effectivenumerical techniques to obtain the solutions of themodel and simulations. Also, we present conditions of existencefor a solution to the proposed epidemicmodel and to calculate the reproduction number in certain state conditionsof the analyzed dynamic system. COVID-19 fractional order model for the case of Wuhan, China, is offered foranalysis with simulations in order to determine the possible efficacy of Coronavirus disease transmission in theCommunity. For this reason, we employed the COVID-19 fractal fractional derivative model in the example ofWuhan, China, with the given beginning conditions. In conclusion, again the mathematical models with fractionaloperators can facilitate the improvement of decision-making for measures to be taken in the management of anepidemic situation.

Novel Investigation of Stochastic Fractional Differential Equations Measles Model via the White Noise and Global Derivative Operator Depending on Mittag-Leffler Kernel

Because of the features involved with their varied kernels,differential operators relying on convolution formulations have been acknowledged as effective mathematical resources for modeling real-world issues.In this paper,we constructed a stochastic fractional framework of measles spreading mechanisms with dual medication immunization considering the exponential decay and Mittag-Leffler kernels.In this approach,the overall population was separated into five cohorts.Furthermore,the descriptive behavior of the system was investigated,including prerequisites for the positivity of solutions,invariant domain of the solution,presence and stability of equilibrium points,and sensitivity analysis.We included a stochastic element in every cohort and employed linear growth and Lipschitz criteria to show the existence and uniqueness of solutions.Several numerical simulations for various fractional orders and randomization intensities are illustrated.

AN EXPLANATION ON FOUR NEW DEFINITIONS OF FRACTIONAL OPERATORS

Fractional calculus has drawn more attentions of mathematicians and engineers in recent years.A lot of new fractional operators were used to handle various practical problems.In this article,we mainly study four new fractional operators,namely the CaputoFabrizio operator,the Atangana-Baleanu operator,the Sun-Hao-Zhang-Baleanu operator and the generalized Caputo type operator under the frame of the k-Prabhakar fractional integral operator.Usually,the theory of the k-Prabhakar fractional integral is regarded as a much broader than classical fractional operator.Here,we firstly give a series expansion of the k-Prabhakar fractional integral by means of the k-Riemann-Liouville integral.Then,a connection between the k-Prabhakar fractional integral and the four new fractional operators of the above mentioned was shown,respectively.In terms of the above analysis,we can obtain this a basic fact that it only needs to consider the k-Prabhakar fractional integral to cover these results from the four new fractional operators.

An Intelligent MCGDM Model in Green Suppliers Selection Using Interactional Aggregation Operators for Interval-Valued Pythagorean Fuzzy Soft Sets

Green supplier selection is an important debate in green supply chain management(GSCM),attracting global attention from scholars,especially companies and policymakers.Companies frequently search for new ideas and strategies to assist them in realizing sustainable development.Because of the speculative character of human opinions,supplier selection frequently includes unreliable data,and the interval-valued Pythagorean fuzzy soft set(IVPFSS)provides an exceptional capacity to cope with excessive fuzziness,inconsistency,and inexactness through the decision-making procedure.The main goal of this study is to come up with new operational laws for interval-valued Pythagorean fuzzy soft numbers(IVPFSNs)and create two interaction operators-the intervalvalued Pythagorean fuzzy soft interaction weighted average(IVPFSIWA)and the interval-valued Pythagorean fuzzy soft interaction weighted geometric(IVPFSIWG)operators,and analyze their properties.These operators are highly advantageous in addressing uncertain problems by considering membership and non-membership values within intervals,providing a superior solution to other methods.Moreover,specialist judgments were calculated by the MCGDM technique,supporting the use of interaction AOs to regulate the interdependence and fundamental partiality of green supplier assessment aspects.Lastly,a statistical clarification of the planned method for green supplier selection is presented.

Weighted multilinear p-adic Hardy operators and commutators on p-adic Herz-type spaces

In this paper,we introduce the weighted multilinear p-adic Hardy operator and weighted multilinear p-adic Ces`aro operator,we also obtain the boundedness of these two operators on the product of p-adic Herz spaces and p-adic Morrey-Herz spaces,the corresponding operator norms are also established in each case.Moreover,the boundedness of commutators of these two operators with symbols in central bounded mean oscillation spaces and Lipschitz spaces on p-adic Morrey-Herz spaces are also given.

Application of Specialized Group Management in the Quality Control of Perioperative Nursing

Objective:To explore the role of specialized group management in the quality control of perioperative nursing.Methods:45 surgical nurses from our hospital were selected as the research subjects.Traditional operating room management was adopted from July 2019 to June 2020,and specialized group management was adopted from July 2020 to June 2021.The surgeon’s satisfaction,surgical nurses’core professional competence,and surgical patients’satisfaction were obtained through surveys and the results were analyzed.Results:Surgeon satisfaction before the implementation of specialized group management was significantly lower than after its implementation(P<0.05).Besides,surgical nurses’core professional competency scores before the implementation of specialized group management were significantly lower than after its implementation(P<0.05).Lastly,surgical patients’satisfaction before the implementation of specialized group management was significantly lower than after its implementation(P<0.05).Conclusion:Specialized group management helps to improve the quality of perioperative care and should be applied in clinical practice.

Maximal operators of pseudo-differential operators with rough symbols

Consider a pseudo-differential operator T_(a)f(x)=∫_(R^(n))e^(ix,ζ)a(x,ζ)f(ζ)dζwhere the symbol a is in the rough Hormander class L^(∞)S_(ρ)^(m)with m∈R andρ∈[0,1].In this note,when 1≤p≤2,if n(ρ-1)/p and a∈L^(∞)S_(ρ)^(m),then for any f∈S(R^(n))and x∈R^(n),we prove that M(T_(a)f)(x)≤C(M(|f|^(p))(x))^(1/p) where M is the Hardy-Littlewood maximal operator.Our theorem improves the known results and the bound on m is sharp,in the sense that n(ρ-1)/p can not be replaced by a larger constant.

Hydrogeochemical Characterization of Groundwater from Fissured Aquifers in the Angovia Mine Operating Permit Area (Central-West Côte d’Ivoire)

The main objective of this study is to determine the hydrogeochemical specificities of the groundwater of the Angovia mine operating permit, located in the Yaouré mountains in the center-west of Côte d’Ivoire. To do so, descriptive and multivariate statistical analysis methods with the SOM (Self Organizing Maps) algorithm were applied to the physicochemical parameters of 17 boreholes using the calcite (ISC) and dolomite (ISD) saturation indices. The results obtained have shown that the groundwater in the Angovia mine operating permit area has an average temperature of 27.52°C (long rainy season) and 27.87&degC (long dry season) and has an average pH of 7.09 ± 0.35 during the main rainy season and 7.32 ± 0.35 during the main dry season. They are mineralized with an average electrical conductivity of 505.98 ± 302.85 μS/cm during the long rainy season and with 450.33 ± 233.74 μS/cm as average during the long dry season. The main phenomena at the origin of groundwater mineralization are water residence time, oxidation-reduction and surface inflow. The study of the relative age of the water shows that the groundwater in the Angovia mine operating permit area is mainly undersaturated with respect to calcite and dolomite. They are therefore very old in the aquifer with a slow circulation speed during the long rainy season and the long dry season.

Appropriate Combination of Crossover Operator and Mutation Operator in Genetic Algorithms for the Travelling Salesman Problem

Genetic algorithms(GAs)are very good metaheuristic algorithms that are suitable for solving NP-hard combinatorial optimization problems.AsimpleGAbeginswith a set of solutions represented by a population of chromosomes and then uses the idea of survival of the fittest in the selection process to select some fitter chromosomes.It uses a crossover operator to create better offspring chromosomes and thus,converges the population.Also,it uses a mutation operator to explore the unexplored areas by the crossover operator,and thus,diversifies the GA search space.A combination of crossover and mutation operators makes the GA search strong enough to reach the optimal solution.However,appropriate selection and combination of crossover operator and mutation operator can lead to a very good GA for solving an optimization problem.In this present paper,we aim to study the benchmark traveling salesman problem(TSP).We developed several genetic algorithms using seven crossover operators and six mutation operators for the TSP and then compared them to some benchmark TSPLIB instances.The experimental studies show the effectiveness of the combination of a comprehensive sequential constructive crossover operator and insertion mutation operator for the problem.The GA using the comprehensive sequential constructive crossover with insertion mutation could find average solutions whose average percentage of excesses from the best-known solutions are between 0.22 and 14.94 for our experimented problem instances.

Wigner function of optical cumulant operator and its dissipation in thermo-entangled state representation

To conveniently calculate the Wigner function of the optical cumulant operator and its dissipation evolution in a thermal environment, in this paper, the thermo-entangled state representation is introduced to derive the general evolution formula of the Wigner function, and its relation to Weyl correspondence is also discussed. The method of integration within the ordered product of operators is essential to our discussion.

Operations Improvement Function and Organisational Adaptability of Petroleum Tank Farms in South-South, Nigeria

The study examined the nexus between operations improvement function (dimensioned by contingency planning, benchmarking and continuous improvement processes) and organisational adaptability of Petroleum tank farms in South-South, Nigeria. The contingency theory and the theory of routine dynamics underpinned the study, and positivism was the underlying philosophy. The study adopted the cross-sectional survey through the use of questionnaire. 820 middle and top-level managers constituted the elements of the population, and the Krejcie & Morgan’s formula was used to determine the sample size of 262 respondents. Structural Equation Modeling was deployed to test the hypotheses at a 0.05 significance level. The results showed that contingency planning;benchmarking and continuous improvement processes all have a significant positive relationship with organisational adaptability of Petroleum tank farms in South-South, Nigeria. The study concludes that Petroleum tank farms’ operations should focus on the adoption of contingency planning, benchmarking and continuous improvement processes to enhance organisational adaptability. Therefore, it is recommended that the management of Petroleum tank farms should put in place mechanisms to advance continuous improvement processes by allocating the necessary amount of resources, such as energy, time and money, in order to promote the continuous development of the continuous improvement systems. Furthermore, managers of Petroleum tank farms should make better the adoption of contingency planning, ensuring that there is as much necessary training and information for employees on how to act during a crises situation, in order to evaluate safety and prepare in advance for recovery from disasters.

Arithmetic Operations of Generalized Trapezoidal Picture Fuzzy Numbers by Vertex Method

In this article, we define the arithmetic operations of generalized trapezoidal picture fuzzy numbers by vertex method which is assembled on a combination of the (α, γ, β)-cut concept and standard interval analysis. Various related properties are explored. Finally, some computations of picture fuzzy functions over generalized picture fuzzy variables are illustrated by using our proposed technique.