A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Surface air temperature change in the Wuyi Mountains,southeast China

Detecting changes in surface air temperature in mid-and low-altitude mountainous regions is essential for a comprehensive understanding of warming trend with altitude.We use daily surface air temperature data from 64 meteorological stations in Wuyi Mountains and its adjacent regions to analyze the spatio-temporal patterns of temperature change.The results show that Wuyi Mountains have experienced significant warming from 1961 to 2018.The warming trend of the mean temperature is 0.20℃/decade,the maximum temperature is 0.17℃/decade,and the minimum temperature is 0.26℃/decade.In 1961-1990,more than 63%of the stations showed a decreasing trend in annual mean temperature,mainly because the maximum temperature decreased during this period.However,in 1971-2000,1981-2010 and 1991-2018,the maximum,minimum and mean temperatures increased.The fastest increasing trend of mean temperature occurred in the southeastern coastal plains,the quickest increasing trend of maximum temperature occurred in the northwestern mountainous region,and the increase of minimum temperature occurred faster in the southeastern coastal and northwestern mountainous regions than that in the central area.Meanwhile,this study suggests that elevation does not affect warming in the Wuyi Mountains.These results are beneficial for understanding climate change in humid subtropical middle and low mountains.

Data-driven methods for predicting the representative temperature of bridge cable based on limited measured data

Cable-stayed bridges have been widely used in high-speed railway infrastructure.The accurate determination of cable’s representative temperatures is vital during the intricate processes of design,construction,and maintenance of cable-stayed bridges.However,the representative temperatures of stayed cables are not specified in the existing design codes.To address this issue,this study investigates the distribution of the cable temperature and determinates its representative temperature.First,an experimental investigation,spanning over a period of one year,was carried out near the bridge site to obtain the temperature data.According to the statistical analysis of the measured data,it reveals that the temperature distribution is generally uniform along the cable cross-section without significant temperature gradient.Then,based on the limited data,the Monte Carlo,the gradient boosted regression trees(GBRT),and univariate linear regression(ULR)methods are employed to predict the cable’s representative temperature throughout the service life.These methods effectively overcome the limitations of insufficient monitoring data and accurately predict the representative temperature of the cables.However,each method has its own advantages and limitations in terms of applicability and accuracy.A comprehensive evaluation of the performance of these methods is conducted,and practical recommendations are provided for their application.The proposed methods and representative temperatures provide a good basis for the operation and maintenance of in-service long-span cable-stayed bridges.

Assessment of the Spatio-Temporal Trends of Annual Extreme Temperature Indices over Tanzania during the Period of 1982-2022

Extreme weather and climatic phenomena, such as heatwaves, cold waves, floods and droughts, are expected to become more common and have a significant impact on ecosystems, biodiversity, and society. Devastating disasters are mostly caused by record-breaking extreme events, which are becoming more frequent throughout the world, including Tanzania. A clear global signal of an increase in warm days and nights and a decrease in cold days and nights has been observed. The present study assessed the trends of annual extreme temperature indices during the period of 1982 to 2022 from 29 meteorological stations in which the daily minimum and maximum data were obtained from NASA/POWER. The Mann-Kendall and Sen slope estimator were employed for trend analysis calculation over the study area. The analyzed data have indicated for the most parts, the country has an increase in warm days and nights, extreme warm days and nights and a decrease in cold days and nights, extreme cold days and nights. It has been disclosed that the number of warm nights and days is on the rise, with the number of warm nights trending significantly faster than the number of warm days. The percentile-based extreme temperature indices exhibited more noticeable changes than the absolute extreme temperature indices. Specifically, 66% and 97% of stations demonstrated positive increasing trends in warm days (TX90p) and nights (TN90p), respectively. Conversely, the cold indices demonstrated 41% and 97% negative decreasing trends in TX10p and TN10p, respectively. The results are seemingly consistent with the observed temperature extreme trends in various parts of the world as indicated in IPCC reports.

Temperature field test and prediction using a GA-BP neural network for CRTS Ⅱ slab tracks

The CRTS Ⅱ slab track, which is connected in a longitudinal direction, is one of the main ballastless tracks in China, with approximately 7365 km of operational track. Temperature loading is a very vital factor leading to slab track damages such as warping and cracking. While existing research on temperature distribution rests on either site tests in special environments or theoretical analysis, the long-term temperature field characteristics are not clear. Therefore, a long-term temperature field test for the CRTS Ⅱ slab track on bridge-subgrade transition section was conducted to analyze the temperature field. A GA-BP(genetic algorithm optimized back propagation) neural network was trained on the test data to predict the temperature field. The vertical and lateral temperature distributions in four typical days were carried out. We found that the temperature along the track was distributed in a nonlinear manner. This was particularly distinct in the vertical direction for depths of less than 300 mm. The highest and lowest daily temperatures and the daily range of the temperature were analyzed. With the increasing depth, the daily highest temperatures and range of the temperature were smaller, the daily lowest temperatures were higher, and the time corresponding to this peak value appeared later in the day. Both the highest and lowest daily temperature could be predicted using the GA-BP neural network, though the accuracy in predicting the highest temperature was higher than that in predicting the lowest temperature.

Mechanical Analysis of a Multi-Test String in High-Temperature and High-Pressure Deep Wells

The mechanical behavior of the test string in deep wells is generally relatively complex as a result of the high temperature and high pressure,severe dogleg and buckling effects,which in some circumstances can even lead to string failure.Traditional computational methods for the analysis of these behaviors are often inaccurate.For this reason,here a more accurate mechanical model of the test string is introduced by considering variables such as temperature,pressure,wellbore trajectory,and buckling,as well as combining them with the deformation and string constraint conditions brought in by changes in temperature and pressure during the tripping,setting,and test operations.The model is validated by applying it to a specific high-pressure gas well(located in Northeast Sichuan).

Rheological properties of warm mix asphalt binder by DSR test at medium temperature

The rheological properties including the complex modulus G＊ and the phase angle δof matrix and warm mix asphalt （WMA）binders were measured by using the dynamic shear rheometer （DSR ） test at the medium temperature ranging from 16 to 40 ℃,and the relationships between the fatigue factor G＊ sinδand the matrix binder property,WMA additive and test temperature were established.It is found that G＊ decreases with the increasing temperature while δincreases inversely,and G＊ of the asphalt binder with high WMA additive dosage is large,and δis small.G＊sinδexponentially decreases with the increasing temperature and linearly increases with the increase in additive dosage,and the amplitudes of variation are large at low temperatures and high additive dosages.The effect of WMA additive on the rheological property is more remarkable for the matrix asphalt binder with low G＊.Besides,aging has a great effect on the property of matrix asphalt binder,and a slight effect on the interaction between asphalt and additive.The high additive dosage can increase the fatigue cracking potential of the asphalt binder.

Homogeneity Test on Temperature Series in Liaoning Province

By means of SNHT,cumulative-departure and rank test,the homogeneity test of annual mean temperature series from 48 stations in Liaoning Province from 1961 to 2008 was carried out,and the reasons for heterogencity formation were analyzed combining with the historical evolution data of these stations.The results showed that all of the three methods were valid for the heterogencity test of temperature series,and there was certain complement between rank test and SNHT test.Station relocation was the main reason for temperature heterogencity,and heterogencity breakpoint mainly appeared in 1970s.

The Coordinated Influence of Indian Ocean Sea Surface Temperature and Arctic Sea Ice on Anomalous Northeast China Cold Vortex Activities with Different Paths during Late Summer

The Northeast China cold vortex(NCCV)during late summer(from July to August)is identified and classified into three types in terms of its movement path using machine learning.The relationships of the three types of NCCV intensity with atmospheric circulations in late summer,the sea surface temperature(SST),and Arctic sea ice concentration(SIC)in the preceding months,are analyzed.The sensitivity tests by the Community Atmosphere Model version 5.3(CAM5.3)are used to verify the statistical results.The results show that the coordination pattern of East Asia-Pacific(EAP)and Lake Baikal high pressure forced by SST anomalies in the North Indian Ocean dipole mode(NIOD)during the preceding April and SIC anomalies in the Nansen Basin during the preceding June results in an intensity anomaly for the first type of NCCV.While the pattern of high pressure over the Urals and Okhotsk Sea and low pressure over Lake Baikal during late summer-which is forced by SST anomalies in the South Indian Ocean dipole mode(SIOD)in the preceding June and SIC anomalies in the Barents Sea in the preceding April-causes the intensity anomaly of the second type.The third type is atypical and is not analyzed in detail.Sensitivity tests,jointly forced by the SST and SIC in the preceding period,can well reproduce the observations.In contrast,the results forced separately by the SST and SIC are poor,indicating that the NCCV during late summer is likely influenced by the coordinated effects of both SST and SIC in the preceding months.

Normalized fatigue properties of asphalt mixture at various temperatures

This study normalized the mixture's fatigue behavior at various temperatures,and the strength and fatigue tests of the mixture were conducted.The stress state of the asphalt mixture includes direct tensile,uniaxial compression,and indirect tensile.The Desai yield surface and fatigue path were proposed.And a normalized fatigue characteristics model of the mixture was established.The following conclusions were obtained.With the increases in the loading rate,the strength of the asphalt mixture increased.As the temperature increases,the strength of the mixture is reduced.At various temperatures and rates,the strength forms a closed curved surface.The Desai strength yield surface was established,which forms a closed curved surface.When the loading rate and temperature are below a certain critical line,the asphalt mixture will not undergo strength damage.At a fixed stress state,the fatigue damage path of the mixture was determined.The stress ratio was determined considering the influence of the loading rate.In this way,a normalized model can be described to express the asphalt mixture fatigue properties at various temperatures and stress levels.For the asphalt mixture in an indirect tensile state,the normalized fatigue equation parameter is 4.09.This model is more suitable for reflecting the viscous-elastic behavior of the mixtures than the fatigue equation determined by the notional stress ratio.

Self-Sensing Test Method for the Temperature of Piezoelectric Stacks

A self-sensing test method for the temperature of piezoelectric stack,based on the high correlation between the static capacitance and the stack temperature,is proposed in order to construct a self-sufficient methodology of temperature measurement. Firstly,a theoretical model of static capacitance of the piezoelectric stack under preload was set up,and the influence of preload on the static capacitance was analyzed. Secondly,the correctness of the model was verified by static capacitance test experiments under various preloading conditions. Finally, the temperature measurement experiments at low-temperature stage for two kinds of piezoelectric stacks,namely the lowtemperature-resistant piezoelectric stack and conventional piezoelectric stack, were conducted under various preloading conditions using a polynomial fitting method. The results,which validate the accuracy of the test method,show that the maximum temperature deviations of the two kinds of piezoelectric stack are 3.9 ℃ and 2.8 ℃,respectively,when the preload force is close to the specified value. The test method uses the piezoelectric stack itself as a temperature sensor,which does not require additional equipment for temperature sensing,so that the space and equipment cost could be economized. And the test for static capacitance is concise and convenient,which indicates that in the cooling process,a concise and efficient test of the temperature of the piezoelectric stack could be realized so as to grasp the current temperature change in time.

Temperature-controlled triaxial compression/creep test device for thermodynamic properties of soft sedimentary rock and corresponding theoretical prediction

In deep geological disposal of high-level nuclear waste,one of the most important subjects is to estimate long-term stability and strength of host rock under high temperature conditions caused by radioactive decay of the waste.In this paper,some experimental researches on the thermo-mechanical characteristics of soft sedimentary rock have been presented.For this reason,a new temperature-controlled triaxial compression and creep test device,operated automatically by a computer-controlled system,whose control software has been developed by the authors,was developed to conduct the thermo-mechanical tests in different thermal loading paths,including an isothermal path.The new device is proved to be able to conduct typical thermo-mechanical element tests for soft rock.The test device and the related testing method were introduced in detail.Finally,some test results have been simulated with a thermo-elasto-viscoplastic model that was also developed by the authors.

Skin Sensitive Difference of Human Body Sections under Clothing— Smirnov Test of Skin Surface Temperatures' Dynamic Changing

Skin sensitive difference of human body sections under clothing is the theoretic foundation of thermal insulation clothing design. By a new method of researching on clothing comfort perception, the skin temperature live changing procedure of human body sections affected by the same cold stimulation is inspected. Furthermore with the Smirnov test the skin temperatures dynamic changing patterns of main human body sections are obtained.

Site-testing at Muztagh-ata site Ⅲ:temperature inversion in surface-layer atmosphere

In this article,we present detailed seasonal,monthly and daily statistics of temperature difference in the surface layer at the Muztagh-ata site based on the temperature measurements at two heights of 2 m and6 m.We find that temperature inversion occurs frequently at our site during nighttime,especially during the cold season.Strong temperature inversion always represents stable atmospheric turbulence,which is crucial for an optical observatory.By analyzing the behavior of temperature inversion and its correlation with wind and cloud amount,one conclusion can be made that radiation inversion is the main reason for the existence of temperature inversion in the surface-layer at the Muztagh-ata site.

Experimental study on temperature stress calculation and temperature control optimization of concrete based on early age parameters

Temperature control curve is the key to achieving temperature control and crack prevention of high concrete dam during construction,and its rationality depends on the accurate measurement of temperature stress.With the simulation testing machine for the temperature stress,in the present study,we carried out the deformation process tests of concrete under three temperature curves:convex,straight and concave.Besides,we not only measured the early-age elastic modulus,creep parameters and stress process,but also proposed the preferred type.The results show that at early age,higher temperature always leads to greater elastic modulus and smaller creep.However,the traditional indoor experiments have underestimated the elastic modulus and creep development at early age,which makes the calculated value of temperature stress too small,thus increasing the cracking risk.In this study,the stress values of the three curves calculated based on the strain and early-age parameters are in good agreement with the temperature stress measured by the temperature stress testing machine,which verifies the method accuracy.When the temperature changes along the concave curve,the law of stress development is in consistent with that of strength.Under this condition,the stress fluctuation is small and the crack prevention safety of the concave type is higher,so the concave type is better.The test results provide a reliable basis and support for temperature control curve design and optimization of concrete dams.

Mechanical properties of high-strength concrete subjected to high temperature by stressed test

Recently, the effects of high temperature on compressive strength and elastic modulus of high strength concrete were experimentally investigated. The present study is aimed to study the effect of elevated temperatures ranging from 20 ℃ to 700 ℃ on the material mechanical properties of high-strength concrete of 40, 60 and 80 MPa grade. During the strength test, the specimens are subjected to a 25% of ultimate compressive strength at room temperature and sustained during heating, and when the target temperature is reached, the specimens are loaded to failure. The tests were conducted at various temperatures (20-700 ℃) for concretes made with W/B ratios of 46%, 32% and 25%, respectively. The results show that the relative values of compressive strength and elastic modulus decrease with increasing compressive strength grade of specimen.

A Study on Extreme Temperature and Climate Events in Zhejiang Region of East China

The extreme high temperature anomaly (EHTA) events in a region are one of the most important climatic parameters to make climate assessment of the trend of regional climate change. The diagnosis and analysis of the EHTA event in Zhejiang Province in East China in 2022 show that the event has set the rarest record in this region in the past 71 years from both time and space perspectives. The results of Mann-Kendall trend analysis showed that the mean annual high temperature days in Zhejiang Province had a sudden change. The sudden change occurred around 2004, and the trend was rising after the sudden change.

Temperature Control and Simulation of Temperature-Altitude Test System

The temperature-Altitude Test System(TATS) supplies various testing environments.The traditional PID method controls the temperature in TATS Temperature-Pressure Cabin(TPC) over a long adjusting time and with a large overshoot.In order to solve this problem simply,a temperature control strategy with temperature difference corresponding factors is presented through a dynamic analysis and modeling of TPC temperature change.The TPC temperature descending process is simulated,and the results show that this control strategy can allot the proportion of PID heating controller and PID cooling controller in the whole control process and TPC temperature can be controlled at a set point quickly and effectively.

TENSILE TESTING OF C/C COMPOSITES AT HIGH TEMPERATURES

The tensile properties of three different carbonfiberreinforced carbon composites (C/C), mat C/C, 2D laminate and 4D C/C, were investigated under the combined influence of temperature and loading rate. From the experiments the following could be concluded: loading rate between 10-1-10 mm/min was valid; the fracture stress of the three kinds of C/C composites increased with increasing temperature in the range from room temperature to 1900, and the initial modulus of 2D laminate C/C composites increased with the increase of temperature up to 2000.

Temperature effects on the mechanical properties of slates in triaxial compression test

High geothermal temperatures appear to be unfavorable for the construction of tunnels in slate rocks with high overburden. To investigate the mechanical characteristics of slates at various levels of geothermal temperature, conventional triaxial compression tests at different levels of confining stress were carried out at 4 different temperatures from 20℃ to 120℃. The obtained results show high confining pressures weaken the thermal effects on rock mechanical characteristics while higher temperatures enhance the effect of confining pressure.At higher levels of confining stress the thermal effects on the rock strength characteristics decrease. The higher the temperature, the larger is the effect of confining pressure on the mechanical characteristics of the slate. Increase of temperature leads to a decrease of the peak strength but increases the deformability and ductility of the slate, the thermo effect on the peak strength and Poisson's ratio is larger than on the elastic modulus. Higher temperatures reduce the shear strength of slate, the decrease is mainly caused by a decrease of the cohesion. In general, the slate samples fail in shear failure.