A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Study on a conical bearing for acceleration-sensitive equipment

Seismic isolation effectively reduces seismic demands on building structures by isolating the superstructure from ground vibrations during earthquakes.However,isolation strategies give less attention to acceleration-sensitive systems or equipment.Meanwhile,as the isolation layer’s displacement grows,the stiffness and frequency of traditional rolling and sliding isolation bearings increases,potentially causing self-centering and resonance concerns.As a result,a new conical pendulum bearing has been selected for acceleration-sensitive equipment to increase self-centering capacity,and additional viscous dampers are incorporated to enhance system damping.Moreover,the theoretical formula for conical pendulum bearings is supplied to analyze the device’s dynamic parameters,and shake table experiments are used to determine the proposed device’s isolation efficiency under various conditions.According to the test results,the newly proposed devices have remarkable isolation performance in terms of minimizing both acceleration and displacement responses.Finally,a numerical model of the isolation system is provided for further research,and the accuracy is demonstrated by the aforementioned experiments.

New Explorations of Laboratory and On-site Testing of Broadband Seismographs in China

According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.

Machines and Testing Equipment

National Quality Supervision ＆ Inspection Center for Refractories Business scope： Selective examination for national quality supervision; Identification of production license; Arbitration inspection and technical achievements evaluation; Commodities inspection and otherquality inspections .

Application of Using Standard Equipment for Dental Scaler Tip Testing according to ISO 18397

The objective of this work was to study the use of standard equipment in amplitude and frequency tests of dental scaler tip according to ISO 18397.Four types of standard equipment:a laser displacement sensor,a microscope,a tachometer,and an ultrasonic frequency meter,were experimentally investigated to test a tip.The standard laser displacement sensor and the standard microscope were used to test the unloaded amplitude of the scaler tip.It was found that two types of standard equipment were able to measure the unloaded amplitude of the tip.The standard microscope was also employed for the loaded amplitude test.This test was performed by pressing the scaler tip with a load of 1 N,which was measured by a load cell set.The peak-to-peak amplitude found from the test was 116.7 m.The frequency test of the scaler tip was conducted using the standard laser displacement sensor,the tachometer and the ultrasonic frequency meter.All three types of standard equipment were found to be able to test the frequency of the tip without cooling liquid.Nevertheless,only the standard tachometer was capable of measuring the frequency of the tip with cooling liquid applied.

Development of in-situ testing equipment of rock and soil thermophysical properties

A mobile in-situ testing equipment used to detect geotechnical thermophysical properties was developed. The equipment is composed of a heat pump, frequency pumps, an electric tee joint regulator valve, some sensors, an electric control system, data acquisition and control system, which can do tests under the condition of extracting and storing subsurface heat. Applying the line source and the cylinder source heat transfer model, and combining the parameters estimation, the average thermophysieal property parameters of rock and soil will be calculated, which provides the basis for designing the ground source heat pump systems.

An Experience of On-site PD Testing for Condition Monitoring of an 11 kV PILC Cable Insulation System

A cable circuit of a substation in the United Kingdom showed high level of PD activities during a survey using hand hold PD testing equipment. The authors were invited to carry out on-site PD testing experiment to further diagnose and locate the potential problem of the cable system. This paper presents the experience of the present authors carrying out the cable test. Following a brief introduction to the experiment equipments and physical connections, the paper analyses the data collected from the testing, including PD pulse shape analysis, frequency spectrum analysis and phase resolved PD pattern analysis. Associated with PD propagation direction identification, PD source diagnosis and localisation was made. Four different types of sensors, which were adapted during the testing, are shown to have different frequency bandwidths and performed differently. Aider comparing the parameters of the sensor and the PD signals detected by individual sensor, optimal PD monitoring bandwidth for cable system is suggested.

Optimization of dynamic sequential test strategy for equipment health management

Testing is the premise and foundation of realizing equipment health management （EHM）. To address the problem that the static periodic test strategy may cause deficient test or excessive test, a dynamic sequential test strategy （DSTS） for EHM is presented. Considering the situation that equipment health state is not completely observable in reality, a DSTS optimization method based on partially observable semi-Markov decision pro- cess （POSMDP） is proposed. Firstly, an equipment health state degradation model is constructed by Markov process, and the control limit maintenance policy is also introduced. Secondly, POSMDP is formulated in great detail. And then, POSMDP is converted to completely observable belief semi-Markov decision process （BSMDP） through belief state. The optimal equation and the corresponding optimal DSTS, which minimize the long-run ex- pected average cost per unit time, are obtained with BSMDP. The results of application in complex equipment show that the proposed DSTS is feasible and effective.

Modeling and Simulation for Complex Repairable System with Built-in Test Equipment

In order to research the effects of built-in test(BIT) on the system and select BITand test strategy,the complex repairable systems with BITequipment are modeled and simulated by using Simulink.Based on the model,the influences of different built-in test equipments,maintenance time and error probabilities on the system usability are evaluated.The simulation results showthat they effect on the system differently.The simulation method of complex system based on Simulink provides a technique approach to research the effects of BITon the system and select BITand test strategy.

Automatic Extraction Method of Weld Weak Defect Features for Ultra-High Voltage Equipment

To solve the problems of low precision of weak feature extraction,heavy reliance on labor and low efficiency of weak feature extraction in X-ray weld detection image of ultra-high voltage(UHV)equipment key parts,an automatic feature extraction algorithm is proposed.Firstly,the original weld image is denoised while retaining the characteristic information of weak defects by the proposed monostable stochastic resonance method.Then,binarization is achieved by combining Laplacian edge detection and Otsu threshold segmentation.Finally,the automatic identification of weld defect area is realized based on the sequential traversal of binary tree.Several characteristic analysis dimensions are established for weld defects of UHV key parts,including defect area,perimeter,slenderness ratio,duty cycle,etc.The experiment using theweld detection image of the actual production site shows that the proposedmethod can effectively extract theweak feature information ofweld defects and further provide reference for decision-making.

Research of in-situ hydraulic test method by using double packer equipment

Double packer equipment for hydraulic test can be used to measure pressure of test zone directly, and it is frequently used to perform many kinds of hydraulic tests and take groundwater sample from borehole. The test method of this equipment mainly includes the test design, implementation, interpretation and synthetic analysis. By adopting the double packer equipment for hydraulic test, the parameter distribution of rock permeability along borehole can be acquired, as well as the connectivity, water conductivity and water bearing capacity of the disclosed structure and the chemical characteristics of the deep groundwater. It is a necessary method for the research and evaluation of the complex hypotonicity terrace site selection under geological conditions. This method is not only suitable for the geological disposal of high level radioactive waste, but also can be used in the site selection of underground facilities such as storage of petroleum and carbon dioxide. Meanwhile, it has a good application prospect in other hydrogeological investigation fields.

Study on rate ripple and its adaptive suppression method in inertia guidance test equipment

This paper discusses causes of the rate ripple in inertia guidance test equipment IGET, systematically analyses their effects an the rate ripple in IGTE. The analysis result shows： The rate ripple caused by the periodic errors of inductosyn and angular encoder is higher at high speed than that caused by magnetic ripple torque and friction torque, and it cannot be eliminated by adjusting control parameters of the system. And based on the nonlinear adaptive control system theory, the paper puts forward a new control system scheme to eliminate the rate ripple caused by the periodic errors of inductosyn and angular encoder, develops the adaptive control rules and makes simulation and test. Experimental result shows a significant improvement on those tables for the period disturbs under the system scheme designed. By this plan, with the input of rate 200°/s, the rate ripple falls from 5°/s to 0. 4°/s within about 6s adaptive adjustment time, being a twelfth of before adaptation, which can not be reached by common classical controls. The experimental results conform with the simulation, which proves the validity and practicability of the plan.

Test Equipment Selection and Deployment of Materiel System Based on Grey Situation Decision

The situation is constructed when the tests are considered as events and the spare test equipments are considered as strategies. The model of grey situation decision for the test equipment selection and deployment （TESD） is founded. Through analyzing each decision objectives, their relative weights are calculated via analytic hierarchy process （AHP）. The grey situation effect matrix under each objective is computed so that the comprehensive effect measure matrix can be obtained when all of the objectives are traded off by their weights. Finally, the decision-maker can select the optimal situations by the value of the measures and such situations form the overall concept for TESD. The paper combines the AHP with grey situation decision to select and deploy the test equipments optimally. Our experimental results show that the proposed method is effective and efficient.

Making your own Automatic Test Equipment has never been easier

Using Planet ATE's SOC 'all-in-one' pin electronics solutions it is easy to make your owntester, ATE or to modify load boards for more advanced test capabilities.

Design of multi-section luffing apple pruning equipment and cutting test of the key components

In order to reduce the labor intensity and improve the efficiency of pruning,this study designed a multi-section luffing apple pruning equipment suitable for apple orchards in hilly and mountainous areas of China.In the essay,the structure and working principle of the equipment are expounded,the cutter was analyzed,and the parameters were determined.Using the self-made test bench to simulate the field experiment,this study selected the ratio of cutting speed to feeding speed and feeding speed as the influencing factors and took the cut-off rate,cutting efficiency and cutting power as the objectives to carry out single factor experiment and quadratic regression orthogonal experiment.The test results show that the cutting power increases with the increase of ratio of cutting speed to feeding speed,and the cut-off rate and cutting efficiency first increase and then decrease.As the feeding speed increases,the cutting efficiency and power increase,and the cut-off rate decreases.When the cutoff rate was greater than 80%,the optimal combination of operating parameters was the feeding speed of 0.55 m/s and ratio of cutting speed to feeding speed of 1.29.Under this combination,the cut-off rate was 84.9%,the cutting efficiency was 13.1,and the cutting power was 175.7 W.The error between the predicted target value of the model and the average measured target value was less than 7%,so the test results are reliable.According to the obtained optimal operating parameters,the actual cutting experiment of the pruning equipment in the field was carried out,and the cutting rate was 96.3%,which meet the design requirements.This study can also meet the needs of mechanized pruning of fruit trees with different crown shapes,improve the applicability of pruning equipment,and provide support for the development of mechanized pruning equipment in China.

Experimental and analytical studies on multiple tuned mass dampers for seismic protection of porcelain electrical equipment

Porcelain electrical equipment （PEE）, such as current transformers, is critical to power supply systems, but its seismic performance during past earthquakes has not been satisfactory. This paper studies the seismic performance of two typical types of PEE and proposes a damping method for PEE based on multiple tuned mass dampers （MTMD）. An MTMD damping device involving three mass units, named a triple tuned mass damper （TTMD）, is designed and manufactured. Through shake table tests and finite element analysis, the dynamic characteristics of the PEE are studied and the effectiveness of the MTMD damping method is verified. The adverse influence of MTMD redundant mass to damping efficiency is studied and relevant equations are derived. MTMD robustness is verified through adjusting TTMD control frequencies. The damping effectiveness of TTMD, when the peak ground acceleration far exceeds the design value, is studied. Both shake table tests and finite element analysis indicate that MTMD is effective and robust in attenuating PEE seismic responses. TTMD remains effective when the PGA far exceeds the design value and when control deviations are considered.

Random Vibration Analysis of the Electronic Equipment Cabinet

In order to understand the vibration characteristic of system structure of electronic equipment cabinet within the particular vibration frequency,the finite element analysis software-ANSYS is used to simulate the tests of random vibrations of the cabinet system and obtain the isopleths graph of deformation and stress of the cabinet.It can confirm maximum of deformation and stress of the cabinet and position happened.Through more analysis of the frequency response curve,which can confirm harm- ful consequences random vibrations caused and weak link of the cabinet structure.The numerical simulation results are in good a- greement with the experimental results.It shows that this research provides an efficient method for the anti-seismic design and the dynamic optimization design.

Predicting stability of integrated circuit test equipment using upper side boundary values of normal distribution

In response to the growing complexity and performance of integrated circuit(IC),there is an urgent need to enhance the testing and stability of IC test equipment.A method was proposed to predict equipment stability using the upper side boundary value of normal distribution.Initially,the K-means clustering algorithm classifies and analyzes sample data.The accuracy of this boundary value is compared under two common confidence levels to select the optimal threshold.A range is then defined to categorize unqualified test data.Through experimental verification,the method achieves the purpose of measuring the stability of qualitative IC equipment through a deterministic threshold value and judging the stability of the equipment by comparing the number of unqualified data with the threshold value,which realizes the goal of long-term operation monitoring and stability analysis of IC test equipment.

Implementation of a Subjective Visual Vertical and Horizontal Testing System Using Virtual Reality

Subjective visual vertical(SVV)and subjective visual horizontal(SVH)tests can be used to evaluate the perception of verticality and horizontality,respectively,and can aid the diagnosis of otolith dysfunction in clinical practice.In this study,SVV and SVH screen version tests are implemented using virtual reality(VR)equipment;the proposed test method promotes a more immersive feeling for the subject while using a simple equipment configuration and possessing excellent mobility.To verify the performance of the proposed VR-based SVV and SVH tests,a reliable comparison was made between the traditional screen-based SVV and SVH tests and the proposed method,based on 30 healthy subjects.The average results of our experimental tests on the VR-based binocular SVV and SVH equipment were−0.15◦±1.74 and 0.60◦±1.18,respectively.The proposed VR-based method satisfies the normal tolerance for horizontal or vertical lines,i.e.,a±3◦error,as defined in previous studies,and it can be used to replace existing test methods.

Characteristics of Fallen Seed Distribution by Domestic and Imported Aerial Seeding Equipment

In order to explore the seed drop characteristics by aerial seeding equipment,taking aerial seeding for Pinus tabulaeformis as an example,the Gaussian curve fitting and chi-square goodness-of-fit test were carried out on the data of fallen seed distribution,and the seed distribution models of domestic FB-85 and imported PZLM-18 equipment were established.The seeding performance indexes of the two kinds of equipment were calculated and compared by using the model,the existing problems of domestic equipment and their causes are analyzed,and finally,some suggestions for equipment optimization were put forward.The results indicated that the seed drop of the two kinds of equipment showed the characteristics of dense distribution in the middle and sparse distribution on both sides,and followed the Gaussian distribution as a whole;compared with PZLM-18,FB-85 had better seeding performance,but it also had the problem of uneven seed distribution;in addition to the influence of aircraft flow field,the fishtail structure design of diffuser is another important reason for the uneven seed distribution of domestic equipment;without changing the fishtail structure design,it is suggested that the principle of cross-superposition of two seeding belts should be used to replace a single large-size diffuser with two small-size diffusers,which can reduce the number of seeds in the middle and increase the number of seeds on both sides,so as to improve the uniformity of seed distribution.