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The Effect of Key Nodes on theMalware Dynamics in the Industrial Control Network
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作者 Qiang Fu JunWang +1 位作者 Changfu Si Jiawei Liu 《Computers, Materials & Continua》 SCIE EI 2024年第4期329-349,共21页
As industrialization and informatization becomemore deeply intertwined,industrial control networks have entered an era of intelligence.The connection between industrial control networks and the external internet is be... As industrialization and informatization becomemore deeply intertwined,industrial control networks have entered an era of intelligence.The connection between industrial control networks and the external internet is becoming increasingly close,which leads to frequent security accidents.This paper proposes a model for the industrial control network.It includes a malware containment strategy that integrates intrusion detection,quarantine,and monitoring.Basedonthismodel,the role of keynodes in the spreadofmalware is studied,a comparisonexperiment is conducted to validate the impact of the containment strategy.In addition,the dynamic behavior of the model is analyzed,the basic reproduction number is computed,and the disease-free and endemic equilibrium of the model is also obtained by the basic reproduction number.Moreover,through simulation experiments,the effectiveness of the containment strategy is validated,the influence of the relevant parameters is analyzed,and the containment strategy is optimized.In otherwords,selective immunity to key nodes can effectively suppress the spread ofmalware andmaintain the stability of industrial control systems.The earlier the immunization of key nodes,the better.Once the time exceeds the threshold,immunizing key nodes is almost ineffective.The analysis provides a better way to contain the malware in the industrial control network. 展开更多
关键词 key nodes dynamic model industrial control network SIMULATION
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Sequence Difference of Mitochondrial DNA Control Region and Genetic Differentiation of Cervinae in China 被引量:1
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作者 涂剑锋 邢秀梅 +2 位作者 徐佳萍 杨颖 杨福合 《Agricultural Science & Technology》 CAS 2011年第11期1717-1720,共4页
[Objective] The study aimed at analyzing Genetic Differentiation of Cervinae. [Method] The complete mitochondrial DNA control region of five species of Cervinae was determined by direct DNA sequencing. Homologous sequ... [Objective] The study aimed at analyzing Genetic Differentiation of Cervinae. [Method] The complete mitochondrial DNA control region of five species of Cervinae was determined by direct DNA sequencing. Homologous sequences of others Cervinae were gained from GenBank, and then analyzed through biology software. [Result] The lengths of their control region were 921-1 072 bp, the nucleotide content of T, A, C, and G was 32.1%, 30.2%, 22.7% and 15.0% separately. Genetic distance of each species ranged from 0.062-0.106, which belonged to level of inter-genus. The molecular phylogenetic tree indicated that red deer, skia deer and White-lipped Deer had close relationship, and they constituted one branch with sambar deer. Eld's deer and elk deer constituted the second branch, and hog deer constituted the third branch alone. [Conclusion] Hog deer and fallow deer should be incorporated in Cervus, divergence time is about 1.55-2.60 million years among Cervinae in China. 展开更多
关键词 Cervinae control Region sequence Analysis PHYLOGENY
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A test sequence generation method of zone controller based on timed automata 被引量:4
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作者 SONG Shuang CHEN Yue-dong 《Journal of Measurement Science and Instrumentation》 CAS CSCD 2019年第3期266-276,共11页
In order to solve the problem of artificial generation and low efficiency of test sequences for zone controller (ZC), a model-based automatic generation method of test sequence is proposed. Firstly, the timed automata... In order to solve the problem of artificial generation and low efficiency of test sequences for zone controller (ZC), a model-based automatic generation method of test sequence is proposed. Firstly, the timed automata model is established based on function analysis of the zone controller, and the correctness of the model is verified by UPPAAL. Then by parsing the timed automata model files, state information and transition conditions can be extracted to generate test case sets. Finally, according to the serialization conditions of test cases, the test cases are serialized into test sequences by using the improved depth first search algorithm. A case, the ZC controls the train running within its jurisdiction, shows that the method is correct and can effectively improve the efficiency of test sequence generation. 展开更多
关键词 test sequence zone controller (ZC) timed automata model file parsing case serialization
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Evolution and controlling factors of the gravity flow deposits in the Miocene sequence stratigraphic framework,the Lower Congo-Congo Fan Basin,West Africa 被引量:1
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作者 CHEN Hua LIN Changsong +7 位作者 ZHANG Zhongmin ZHANG Demin LI Ming WU Gaokui ZHU Yixuan XU Hai LU Wenming CHEN Jihua 《Petroleum Exploration and Development》 CSCD 2021年第1期146-158,共13页
To understand the evolution of the Miocene gravity flow deposits in the Lower Congo-Congo Fan Basin,this paper documents the Miocene sequence stratigraphic framework,the depositional characteristics and the controllin... To understand the evolution of the Miocene gravity flow deposits in the Lower Congo-Congo Fan Basin,this paper documents the Miocene sequence stratigraphic framework,the depositional characteristics and the controlling factors of the gravity flow system.Based on the establishment of high-resolution sequence stratigraphic framework,lithofacies characteristics and sedimentary units of the gravity flow deposits in the region are identified by using seismic,well logging and core data comprehensively,and the sedimentary evolution process is revealed and the controlling factors are discussed.The Miocene can be divided into four 3 rd-order sequences(SQ1-SQ4).The gravity flow deposits mainly include siliciclastic rock and pelite.The main sedimentary units include slumping deposits,mass transport deposits(MTD),channel fills,levee-overbank deposits,and frontal lobes.In the Early Miocene(SQ1),mainly gull-wing,weakly restricted to unrestricted depositional channel-overbank complexes and lobes were formed.In the early Middle Miocene(SQ2),W-shaped and weakly restricted erosional-depositional channels(multi-phase superposition)were subsequently developed.In the late Middle Miocene(SQ3),primarily U-shaped and restricted erosional channels were developed.In the Late Miocene(SQ4),largely V-shaped and deeply erosional isolated channels were formed in the study area.Climate cooling and continuous fall of the sea level made the study area change from toe of slope-submarine plain to lower continental slope,middle continental slope and finally to upper continental slope,which in turn affected the strength of the gravity flow.The three times of tectonic uplifting and climate cooling in the West African coast provided abundant sediment supply for the development of gravity flow deposits.Multistage activities of salt structures played important roles in redirecting,restricting,blocking and destroying the gravity flow deposits.Clarifying the characteristics,evolution and controlling factors of the Miocene gravity flow deposits in the Lower Congo-Congo Fan Basin can provide reference for deep-water petroleum exploration in this basin. 展开更多
关键词 gravity flow deposits sequence architecture depositional characteristics controlling factors MIOCENE Lower Congo-Congo Fan Basin
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Design of control sequence of pulses for the population transfer of high dimensional spin 1/2 quantum systems
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作者 Cong Shuang Lou Yuesheng 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2008年第6期1226-1234,共9页
On the basis of the relationship between the Hamiltonian of spin 1/2 quantum system under control and the energy level structure and transitions, a radio frequency pulse sequence is designed using intuitive and half c... On the basis of the relationship between the Hamiltonian of spin 1/2 quantum system under control and the energy level structure and transitions, a radio frequency pulse sequence is designed using intuitive and half counter-intuitive sequences of pulse to transfer the population of the 3-qubit system coherently. The effectiveness of the designed control sequence is verified through the system simulation experiment of the evolution of state. In principle, the design method of the control pulse sequence proposed can be generalized to use in the quantum systems of higher dimension. 展开更多
关键词 quantum system control population transfer pulse sequence design intuitive sequence half counter-intuitive sequence
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ANALYSIS OF THE MUTUAL INFORMATION BETWEEN INPUT AND OUTPUT OF A CLASS OF CLOCK-CONTROLLED SEQUENCES 被引量:3
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作者 Fan Xiubin Li Shiqu(Department of Information Researches, Zhengzhou Information Engineering Institute, Zhengzhou 450002) 《Journal of Electronics(China)》 2000年第2期185-192,共8页
In this paper, the mutual information between clock-controlled input and output sequences is discussed. It is proved that the mutual information is a strictly monotone increasing function of the length of output seque... In this paper, the mutual information between clock-controlled input and output sequences is discussed. It is proved that the mutual information is a strictly monotone increasing function of the length of output sequence, and its divergent rate is gaven. 展开更多
关键词 Clock-controlled sequenceS Mutual information π-class Σ-ALGEBRA
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From Control Theory to Gravitational Waves
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作者 Jean-Francois Pommaret 《Advances in Pure Mathematics》 2024年第2期49-100,共52页
When D:ξ→η is a linear ordinary differential (OD) or partial differential (PD) operator, a “direct problem” is to find the generating compatibility conditions (CC) in the form of an operator D<sub>1:</su... When D:ξ→η is a linear ordinary differential (OD) or partial differential (PD) operator, a “direct problem” is to find the generating compatibility conditions (CC) in the form of an operator D<sub>1:</sub>η→ξ such that Dξ = η implies D<sub>1</sub>η = 0. When D is involutive, the procedure provides successive first-order involutive operators D<sub>1</sub>,...,D<sub>n </sub>when the ground manifold has dimension n. Conversely, when D<sub>1</sub> is given, a much more difficult “inverse problem” is to look for an operator D:ξ→η having the generating CC D<sub>1</sub>η = 0. If this is possible, that is when the differential module defined by D<sub>1</sub> is “torsion-free”, that is when there does not exist any observable quantity which is a sum of derivatives of η that could be a solution of an autonomous OD or PD equation for itself, one shall say that the operator D<sub>1</sub> is parametrized by D. The parametrization is said to be “minimum” if the differential module defined by D does not contain a free differential submodule. The systematic use of the adjoint of a differential operator provides a constructive test with five steps using double differential duality. We prove and illustrate through many explicit examples the fact that a control system is controllable if and only if it can be parametrized. Accordingly, the controllability of any OD or PD control system is a “built in” property not depending on the choice of the input and output variables among the system variables. In the OD case and when D<sub>1</sub> is formally surjective, controllability just amounts to the formal injectivity of ad(D<sub>1</sub>), even in the variable coefficients case, a result still not acknowledged by the control community. Among other applications, the parametrization of the Cauchy stress operator in arbitrary dimension n has attracted many famous scientists (G. B. Airy in 1863 for n = 2, J. C. Maxwell in 1870, E. Beltrami in 1892 for n = 3, and A. Einstein in 1915 for n = 4). We prove that all these works are already explicitly using the self-adjoint Einstein operator, which cannot be parametrized and the comparison needs no comment. As a byproduct, they are all based on a confusion between the so-called div operator D<sub>2</sub> induced from the Bianchi operator and the Cauchy operator, adjoint of the Killing operator D which is parametrizing the Riemann operator D<sub>1</sub> for an arbitrary n. This purely mathematical result deeply questions the origin and existence of gravitational waves, both with the mathematical foundations of general relativity. As a matter of fact, this new framework provides a totally open domain of applications for computer algebra as the quoted test can be studied by means of Pommaret bases and related recent packages. 展开更多
关键词 Differential Operator Differential sequence Killing Operator Riemann Operator Bianchi Operator Cauchy Operator control Theory controllability Elasticity General Relativity
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CONSTRUCTING AND ANALYZING A CLASS OF CONTROLLABLE SEQUENCES
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作者 冯登国 肖国镇 《Journal of Electronics(China)》 1995年第3期259-263,共5页
By using m-sequence over the finite field GF(2m) of degree L to control m-sequence over GF(2m) of degree L, a class of periodic sequences with large linear complexity is constructed. The lower bounds of the linear com... By using m-sequence over the finite field GF(2m) of degree L to control m-sequence over GF(2m) of degree L, a class of periodic sequences with large linear complexity is constructed. The lower bounds of the linear complexity of these sequences are L((L +1)m-Lm). 展开更多
关键词 Linear COMPLEXITY controlLABLE sequence Feedback POLYNOMIAL
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POWER CONTROL WITH CODER-ESTIMATOR SEQUENCES
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作者 Li Xia Wong Wing Shing(Dept. of Electrical Engineering, Shenzhen University, Shenzhen 518060) (Dept. of Information Engineering, the Chinese University of Hong Kong, Shatin, N.T., Hong Kong) 《Journal of Electronics(China)》 1999年第3期263-268,共6页
Power control is an important issue in mobile communication systems and has been studied extensively in the literature. This paper has taken on a new perspective and modeled the problem as a state estimation problem w... Power control is an important issue in mobile communication systems and has been studied extensively in the literature. This paper has taken on a new perspective and modeled the problem as a state estimation problem with finite communication constraints. The problem is solved by using the 1-bit mean coder-estimator sequence. The performance of the coder-estimator sequence is compared with that of the simple feedback power control technique by evaluating their respective fade margins as in commonly done in radio system engineering. 展开更多
关键词 MOBILE communication Power control Coder-estimator sequence
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Comparative Analysis of Mitochondrial Control Region Sequence from Three Flatfish Species(Pleuronectidae)
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作者 YUJianzhong KONGXiaoyu +1 位作者 YUZiniu ZHOULishi 《Journal of Ocean University of China》 SCIE CAS 2005年第1期80-84,共5页
The 5’-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids spe... The 5’-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids species retrieved from GenBank. A phylogenetic tree was constructed based on the partial control region sequences. The results of phyloge- netic analysis are consistent with those of conventional systematics. Compared to previous studies, the structure of the 5’-end of mitochondrial control region was analyzed. The terminal associated sequence motif and its complementary motif were i- dentified at the 5’-end of the sequences. A conserved sequence block, named as CM5’d, was identified in the 5’-end of con- trol region sequences in all Pleuronectids. Another central conserved sequence block, named as CSB-F, was detected in the central conserved blocks. 展开更多
关键词 PLEURONECTIDAE FLATFISH mitochondrial control region terminal associatedsequence conserved sequence block
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The General Configuration of CEV1 Electric Vehicle's Electrical System and the Design of Its Control Sequence
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作者 崔淑梅 Qiu Changhua +1 位作者 Ge Xin CHENG Shukang 《High Technology Letters》 EI CAS 2001年第2期83-86,共4页
The configuration an electric vehicle's electrical system and its control sequence design scheme was discussed. Vehicle management unit (VMU), power management unit (PMU) and battery management unit (BMU) were use... The configuration an electric vehicle's electrical system and its control sequence design scheme was discussed. Vehicle management unit (VMU), power management unit (PMU) and battery management unit (BMU) were used as the control units in the vehicle. VMU was used as master control unit, PMU was used as the traction control unit and BMU was used as battery management unit. The concentrated single module control was found to be on one of its configurations. 展开更多
关键词 Electric vehicle Electrical system sequence control
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Living/controlled polymerization of vinylpyridines into sequence-regulated copolymers by Lewis pair
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作者 Qiankun Ding Wuchao Zhao +2 位作者 Fukuan Li Jianghua He Yuetao Zhang 《Science China Chemistry》 SCIE EI CAS CSCD 2024年第8期2737-2746,共10页
The poly(vinylpyridine)(PVP) based(co)polymers are of particular interest in materials science, due to their multifunctionality and diverse applications. So far, there is no report on the sequence-regulated copolymeri... The poly(vinylpyridine)(PVP) based(co)polymers are of particular interest in materials science, due to their multifunctionality and diverse applications. So far, there is no report on the sequence-regulated copolymerization of vinylpyridines(VPs) and methacrylate monomer in one-step manner yet. Here we designed and synthesized a series of guanidine phosphines as Lewis base(LB), which is combined with bulky organoaluminium to construct Lewis pairs(LPs) for polymerization of VPs. The living/controlled polymerization of 4-vinylpyridine(4-VP) or 2-vinylpyridine(2-VP) can be accomplished with remarkable efficiency by such Lewis pair polymerization(LPP), furnishing polymers with high molecular weight(up to 288 kg/mol) and narrow molecular weight distribution(as low as 1.17). Mechanistic studies reveal the interaction of LPs and formation of zwitterionic intermediates, providing solid evidences to support the proposed polymerization mechanism. More importantly, by simply adjusting the LA dosage, this LPP strategy realizes the unprecedented control over the sequence regulation of 2-VP-based copolymers from gradient to block in one-step manner, regardless of the monomer ratio, which greatly expands the versatility of the LPP. 展开更多
关键词 guanidine phosphine Lewis pair polymerization living/controlled polymerization vinyl pyridine sequence regulation
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Fully Decoupled Branch Energy Balancing Control Method for Modular Multilevel Matrix Converter Based on Sequence Circulating Components
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作者 Zexin Zhao Weijiang Chen +5 位作者 Zhichang Yang Guoliang Zhao Bin Han Yunfei Xu Nianwen Xiang Shulai Wang 《CSEE Journal of Power and Energy Systems》 SCIE EI CSCD 2024年第1期235-247,共13页
The modular multilevel matrix converter(M3C)is a potential frequency converter for low-frequency AC transmission.However,capacitor voltage control of high-voltage and largecapacity M3C is more difficult,especially for... The modular multilevel matrix converter(M3C)is a potential frequency converter for low-frequency AC transmission.However,capacitor voltage control of high-voltage and largecapacity M3C is more difficult,especially for voltage balancing between branches.To solve this problem,this paper defines sequence circulating components and theoretically analyzes the influence mechanism of different sequence circulating components on branch capacitor voltage.A fully decoupled branch energy balancing control method based on four groups of sequence circulating components is proposed.This method can control capacitor voltages of nine branches in horizontal,vertical and diagonal directions.Considering influences of both circulating current and voltage,a cross decoupled control is designed to improve control precision.Simulation results are taken from a low-frequency transmission system based on PSCAD/EMTDC,and effectiveness and precision of the proposed branch energy balancing control method are verified in the case of nonuniform parameters and an unbalanced power system. 展开更多
关键词 Branch energy balancing control decoupled control low-frequency AC transmission modular multilevel matrix converter(M3C) sequence circulating components
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Effect of Sludge Retention Time on Nitrite Accumulation in Real-time Control Biological Nitrogen Removal Sequencing Batch Reactor 被引量:7
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作者 吴昌永 彭永臻 +2 位作者 王淑莹 李晓玲 王然登 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2011年第3期512-517,共6页
In this study,four sequencing batch reactors(SBR),with the sludge retention time(SRT)of 5,10,20 and 40 d,were used to treat domestic wastewater,and the effect of SRT on nitrite accumulation in the biological nitrogen ... In this study,four sequencing batch reactors(SBR),with the sludge retention time(SRT)of 5,10,20 and 40 d,were used to treat domestic wastewater,and the effect of SRT on nitrite accumulation in the biological nitrogen removal SBR was investigated.The real-time control strategy based on online parameters,such as pH,dissolved oxygen(DO)and oxidation reduction potential(ORP),was used to regulate the nitrite accumulation in SBR. The model-based simulation and experimental results showed that with the increase of SRT,longer time was needed to achieve high level of nitritation.In addition,the nitrite accumulation rate(NAR)was higher when the SRT was relatively shorter during a 112-day operation.When the SRT was 5 d,the system was unstable with the mixed liquor suspended solids(MLSS)decreased day after day.When the SRT was 40 d,the nitrification process was significantly inhibited.SRT of 10 to 20 d was more suitable in this study.The real-time control strategy combined with SRT control in SBR is an effective method for biological nitrogen removal via nitrite from wastewater. 展开更多
关键词 biological nitrogen removal nitrite accumulation real time control sludge retention time sequencing batch reactors
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A Genetic Algorithm on Multiple Sequences Alignment Problems in Biology 被引量:3
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作者 Shi Feng, Huang Jing, Mo Zhong-xi, Zheng Hui-rao School of Mathematics and Statistics, Wuhan University, Wuhan 430072, Hubei, China 《Wuhan University Journal of Natural Sciences》 CAS 2002年第2期139-144,共6页
The study and comparison of sequences of characters from a finite alphabet is relevant to various areas of science, notably molecular biology. The measurement of sequence similarity involves the consideration of the p... The study and comparison of sequences of characters from a finite alphabet is relevant to various areas of science, notably molecular biology. The measurement of sequence similarity involves the consideration of the possible sequence alignments in order to find an optimal one for which the “distance” between sequences is minimum. In biology informatics area, it is a more important and difficult problem due to the long length (100 at least) of sequence, this cause the compute complexity and large memory require. By associating a path in a lattice to each alignment, a geometric insight can be brought into the problem of finding an optimal alignment, this give an obvious encoding of each path. This problem can be solved by applying genetic algorithm, which is more efficient than dynamic programming and hidden Markov model using commomly now. 展开更多
关键词 key words sequence comparison biological sequences genetic algorithm
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Multiple UIO-based test sequence generation for distributed systems 被引量:2
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作者 刘文宇 曾红卫 缪淮扣 《Journal of Shanghai University(English Edition)》 CAS 2008年第5期438-443,共6页
In developing distributed systems, conformance testing is required to determine whether an implementation under test (IUT) conforms to its specification. With distributed test architectures involving multiple remote... In developing distributed systems, conformance testing is required to determine whether an implementation under test (IUT) conforms to its specification. With distributed test architectures involving multiple remote testers, testing approaches may become more complicated because of issues known as controllability and observability problems. Based on a finite state machine (FSM) representation of the system's specification, this paper proposes a new method to generate a test sequence utilizing multiple UIO sequences. The method is essentially guided by the way of minimizing the use of external coordination messages and input/output operations. Experiments are given to evaluate the proposed method. 展开更多
关键词 distributed test controlLABILITY OBSERVABILITY finite state machine (FSM) multiplc UIO sequences
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Evaluation of Population Structure, Genetic Diversity and Origin of Northeast Asia Weedy Rice Based on Simple Sequence Repeat Markers 被引量:1
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作者 LI Mao-bai WANG Hui CAO Li-ming 《Rice science》 SCIE CSCD 2015年第4期180-188,共9页
Weedy rice exerts a severe impact on rice production by competing for sunlight, water and nutrients. This study assayed the population structure, genetic diversity and origin of Northeast Asia weedy rice by using 48 s... Weedy rice exerts a severe impact on rice production by competing for sunlight, water and nutrients. This study assayed the population structure, genetic diversity and origin of Northeast Asia weedy rice by using 48 simple sequence repeat markers. The results showed that weedy rice in Northeast Asia had a high genetic diversity, with Shannon's diversity index (I) of 0.748 and the heterozygosity (He) of 0.434. In each regional population, I value varied widely. The widest range of I (0.228-0.489) was observed in the weedy rice of Eastern China, which was larger than that of Northeast China and Korea (0.168-0.270). The F-statistics of regional populations (Fis, Fit and Fst) also showed higher values in the weedy rice of Eastern China than those of Northeast China and Korea All weedy rice accessions were grouped into two clusters in the unweighted pair group method with arithmetic mean cluster analysis dendrogram, namely Eastern China branch and Northeastern China plus Korea branch. There was significant differentiation in genetic characteristics in weedy rice of northeastern and eastern Asia, especially in Eastern China. 展开更多
关键词 weedy rice simple sequence repeat genetic diversity population structure weed control
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An autobias control system for the electro optic modulator used in a quantum key distribution system 被引量:1
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作者 陈文芬 魏正军 +6 位作者 郭莉 侯丽燕 王赓 王金东 张智明 郭建平 刘颂豪 《Chinese Physics B》 SCIE EI CAS CSCD 2014年第8期261-268,共8页
In a quantum key distribution system, it is crucial to keep the extinction ratio of the coherent pulses stable. This means that the direct current bias point of the electro-optic modulator (EOM) used for generating ... In a quantum key distribution system, it is crucial to keep the extinction ratio of the coherent pulses stable. This means that the direct current bias point of the electro-optic modulator (EOM) used for generating coherent pulses must be locked. In this paper, an autobias control system based on a lock-in-amplifier for the EOM is introduced. Its drift information extracting theory and control method are analyzed comprehensively. The long term drift of the extinction ratio of the coherent pulses is measured by a single photon detector, which indicates that the autobias control system is effective for stabilizing the bias point of the EOM. 展开更多
关键词 quantum key distribution electro-optic modulator autobias control lock-in-amplifier
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Molecular Cloning and Nucleotide Sequence of the Attenuated Hog Cholera VirusLapinized Chinese Strain 被引量:1
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作者 Wang Jiafu Zhang Chuyu +3 位作者 Fu Liezhen Wang Ning Huang Qianhua Zhang Pengwei(College of Life Sciences, Wuhan University, Wuhan 430072, China) 《Wuhan University Journal of Natural Sciences》 CAS 1998年第4期499-503,共5页
The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt) including 374 nt and 2... The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt) including 374 nt and 239 nt at the 5′ and 3′-noncoding region, respectively. The complete genome sequence contained one large open reading frame which encoded an amino acid sequence of 3 898 residues with a calculated molecular weight of 437×103. Although there were mostly only small differences between the sequence of the HCLV strain and the published sequences of strains ALD, GPE?, Alfort and Brescia, there was one notable insertion of 12 nucleotides, TTTTCTTTTTTC in the 3′ non-coding region of HCLV strain. 展开更多
关键词 key words hog cholera virus molecular cloning sequence
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Molecular Cloning and NucleotideSequence of Classical SwineFever Virus Strain Shimen 被引量:1
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作者 Huang Qianhua Zhang Chuyu +2 位作者 Wang Jiafu Wang Ning Fu Liezhen(College of Life Sciences, Wuhan University, Wuhan, 430072,China) 《Wuhan University Journal of Natural Sciences》 CAS 1998年第4期504-508,共5页
According to the previously published CSFV sequences, 18 paris of partially overlapping primers which span the entire genome of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was... According to the previously published CSFV sequences, 18 paris of partially overlapping primers which span the entire genome of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was amplified by RT-PCR method from the anticoagulant blood of strain Shimen infected pig. The PCR fragments were cloned into pGEM-T vector respectively and sequenced. The results show that we have obtained the nucleotide sequence of strain Shimen. The viral RNA consists of 12 297 nucleotides including noncoding regions of 373 and 227 bases at the 5′ and 3′ end, respectively, and a single large open reading frame spanning 11 697 nucleotides in the middle, which encodes an amino acid sequence of 3 989 residues with a calculated molecular weight of 437.6×103. The precisely sequencing of 5′ and 3′ termini is undertaking. 展开更多
关键词 key words classical swine fever virus strain Shimen CLONING sequencING
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