Optimization of Ultrasonic-assisted Extraction Process for Polysaccharide from Lactarius deliciosus by Response Surface Methodology and Its Antioxidant Activity

[Objectives]Polysaccharide was extracted from Lactarius deliciosus by ultrasonic-assisted method to improve polysaccharide yield.[Methods]Five variables including extraction temperature,extraction time,ultrasonic power,ultrasonic time and material-to-liquid ratio were selected for single factor experiments.The extraction process of L.deliciosus polysaccharide was optimized by response surface analysis,and its antioxidant activity was evaluated by measuring its total reduction ability and DPPH free radical scavenging capacity.[Results]The optimal extraction conditions were determined as follows:material-to-liquid ratio 1:35 g:ml,ultrasonic power 462 W,ultrasonic time 10 min,extraction time 110 min and extraction temperature 90℃.Under these conditions,the extraction rate of polysaccharide was(10.83±0.03)%.The antioxidant test results showed that when the mass concentration of L.deliciosus polysaccharide was 0.5 mg/ml,its absorbance and DPPH free radical scavenging rate reached their maximum values,which were 3.274%and 41.27%,respectively.The L.deliciosus polysaccharide had good antioxidant properties.[Conclusions]This study provides a theoretical basis for further development and utilization of L.deliciosus polysaccharide in the future.

Optimization of Polysaccharides Extraction from Physalis alkekengi L.Peel and Its Effect on the Expression of Inflammation-Related Proteins in SW620 Cells

Objective:To establish an optimized aqueous extraction process for polysaccharides from Physalis alkekengi L.peel and to preliminarily explore its in vitro anti-inflammatory activity against colorectal cancer SW620 cells.Methods:A single-factor test combined with orthogonal test analysis was used to evaluate the effects of the material-to-liquid ratio,extraction temperature,and extraction time on the yield of polysaccharides from Physalis alkekengi L.peel.The antioxidant activity of the polysaccharides was assessed by analyzing their free radical scavenging ability in vitro,and the anti-inflammatory effect was evaluated using SW620 cells.Results:The optimal extraction conditions were a material-to-liquid ratio of m(g):V(mL)=1:30,an extraction temperature of 100℃,and an extraction time of 40 minutes,with a predicted polysaccharide yield of 25.7%.The polysaccharides from Physalis peruviana peel effectively scavenged DPPH,superoxide anion,and hydroxyl radicals.After treatment with Physalis peruviana polysaccharides,the levels of IL-1β,IL-18,and TNF-αin the cell culture medium were significantly reduced,and the phosphorylation level of P65 protein in SW620 cells was decreased.Conclusion:This extraction method is stable and reliable,and the prepared Physalis alkekengi L.polysaccharides exhibit significant in vitro antioxidant and anti-inflammatory activities.This study provides a theoretical basis for developing drugs for the prevention and treatment of colorectal cancer.

Ultrasonic Extraction of Polysaccharides from Laminaria japonica and Their Antioxidative and Glycosidase Inhibitory Activities

In the present study, ultrasonic extraction technique （UET） is used to improve the yield of polysaccharides from Lami- naria japonica （LJPs）. And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design （BBD） combined with response surface methodology （RSM） is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80℃ and ratio of material to solvent at 1：50 （g mL-1）. Under these optimal ultrasonic extraction conditions, an actual experimental yield （5.75% ＋ 0.3%） is close to the predicted result （5.67%） with no significant difference （P〉0.05）. Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides （LJP） and two major ethanol precipitated fractions （LJP1 and LJP2） are in a concentration-dependent manner. LJP2 （30%-60% ethanol precipitated polysaccharides） possesses the strongest α-glucosidase in- hibitory activity and moderate scavenging activity against hydroxyl radicals （66.09% ±2.19%, 3.0 mg mL-l）. Also, the inhibitory activity against a-glucosidase （59.08% ± 3.79%, 5.0 mg mL-1） is close to that of acarbose （63.99% ± 3.27%, 5.0 mg mL-l）. LJP 1 （30% ethanol precipitated polysaccharides） exhibits the strongest scavenging activity against hydroxyl radicals （99.80%q-0.00%, 3.0mg mL-1） and moderate a-glucosidase inhibitory activity （47.76%± 1.92%, 5.0 mgmL-1）. LJP shows the most remarkable DPPH scav- enging activity （66.20%±0.11%, 5.0mgmL-1） but weakest a-glucosidase inhibitory activity （37.77%±1.30%, 5.0mgmL-1）. How- ever, all these LJPs exert weak inhibitory effects against a-amylase. These results show that UET is an effective method for extract- ing bioactive polysaccharides from seaweed materials. LJP 1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of diabetes. This study provides scientific evidence and advances in the preparation technology and a hypoglycemic activities evaluation method for seaweed polysaccharides, especially glycosidase inhibition in com- bination with an antioxidative activity evaluation method.

A Study on the Ultrasonic Extraction and Antioxidant Activity of Lychee Pericarp Polysaccharides

The aim was to optimize ultrasonic extraction conditions and study antioxidant activities of lychee pericarp polysaccharides. The effects of extraction temperature,time,ultrasonic power,solid-liquid ratio on polysaccharides yield were investigated by single-factor experiment and orthogonal experiment to obtain optimum extraction conditions; the antioxidant activities of lychee pericarp polysaccharides were evaluated by the total antioxidant activity. Optimum extraction process was under extraction temperature of 80 ℃,extraction time of 2. 5 h,ultrasonic power of 180 W,solid-liquid ratio of 1∶ 25 g / m L; the total antioxidant activity was increased with the increasing concentration of polysaccharides. The extraction process is simple and efficient. The extraction rate of lychee pericarp polysaccharides with antioxidant activities is high.

Study on the Optimization of Extraction Technology of Rose laevigate Michx. Polysaccharides and Its in vitro Antioxidant Activity

[Objectives]This study was conducted to optimize the optimal process conditions for extracting polysaccharides from Rose laevigate Michx.with hot water.[Methods]The extraction rate of R.laevigate was used as an evaluation index,and extraction temperature,material-to-liquid ratio,extraction time and extraction times were selected as influencing factors.On the basis of single factor tests,L 9(34)orthogonal tests were carried out for optimization,and the content of polysaccharides was determined by the phenol-sulfuric acid method.The in vitro antioxidant activity of the extracted R.laevigate polysaccharides was studied.[Results]The effects of various influencing factors on the extraction rate of R.laevigate polysaccharides ranked as material-liquid ratio>extraction temperature>extraction times>extraction time.The optimal extraction process was extraction temperature 80℃,extraction time 2 h,and material-to-liquid ratio 1∶25,extraction times 3 times,under which the extraction rate was(9.55±0.12)%.R.laevigate polysaccharides had certain scavenging capacities of DPPH free radicals and hydroxyl free radicals,and its scavenging capacity of DPPH free radicals was better than that of hydroxyl free radicals.[Conclusions]This study provides a theoretical basis for the optimization of the extraction process of R.laevigate polysaccharides and the in-depth development and application of polysaccharides.

Extraction of Pumpkin Polysaccharide by Complex Enzyme Method and Its Antioxidant Research

[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.

Extraction of Water-soluble Polysaccharide from Pumpkin and Its Antioxidant Activity

In this study,an orthogonal experiment was performed to explore the optimal extraction technique of water-soluble polysaccharides from pumpkin.Moreover,the polysaccharides＇ capabilities to scavenge ·OH and DPPH· and their gross reducing power was measured with Vc and BHT as references.In the orthogonal experiment,various factors including temperature,extraction time,solid-liquid ratio,were optimized.The results showed that the polysaccharides extracted from pumpkin are endowed with strong in vitro antioxidant properties.Our study provided references for the extraction of pumpkin polysaccharides and for their use as antioxidants.

Extraction and Antioxidant Functions of Polysaccharides from Seaweed

Dried seaweed powder was used as the raw material to extract polysaccharides by ethanol precipitation after water bath. Extraction temperature, extrac- tion duration, solution pH and solid to liquid ratio were selected as technical parameters which required to be optimized. With the extraction rate of seaweed poly- saccharides as an index, single-factor experiment and orthogonal experiment were carried out to determine the optimal extraction technique. Seaweed polysaceharides were extracted with the optimal technical parameters to analyze the biological activities. Savage method was used to process seaweed polysaceharide extract. In vitro biological activities of preliminarily purified seaweed polysaeeharides were investigated successively, including reducing ability, antioxidant ability and antibacterial activity. The results showed that there was a positive correlation between polysaccharide concentration and its reducing ability, antioxidant ability and antibacterial activity. Furthermore, there was also a correlation among the reducing ability, antioxidant ability and antibacterial activity of seaweed polysaecharides; the relation- ship between each two of these three indicators was similar to positive linear correlation.

Extraction and Antioxidant Activity Analysis of Crude Polysaccharides from Wild Lactarius volemus Fr. in Yunnan Province

[ Objective ] This study aimed to investigate the optimal extraction process of crude polysaccharides from wild Lactarius volemus Fr. in Yunnan Province and preliminarily analyzed its antioxidant activity in vitro. [ Method ] With water extraction and alcohol precipitation method, the optimal conditions for extracting crude polysaccharides from wild L. volemus Ft. were screened by single-factor and orthogonal experiments. The antioxidant activity of the extracted crude polysac- charides was determined with DPPH assay. [ Result ] The optimal conditions for pigment removal with activated carbon were： activated carbon amount of 20 g/L, water bath time of 40 min, water bath temperature of40 ℃ ; the optimal conditions for extracting crude polysaccharides from wild L. vo/emus Fr. with hot water ex- traction method were： hot water extraction time of 3 h, solid-liquid ratio of 1： 45, extraction frequency of twice. Under the optimized extraction conditions, the yield of crude polysaccharides was 21.33 mg/g. In addition, the antioxidant activity of 0. 665 mg/ml crude polysaccharides was 52.46% ; the amount of crude polysac- eharides was proportional to the antioxidant activity. [ Conclusion] Hot water extraction method can be used as a high-efficiency extraction technology of crude pol- ysaecharides from wild L. volemus Fr. with simple operation and low costs. Crude polysaccharides extracted from L. volemus Ft. exhibited certain antioxidant activi- ty in vitro.

Polysaccharides in Sipunculus nudus: Extraction Condition Optimization and Antioxidant Activities

Marine organisms constitute unlimited resource of bioactive substances due to their high biodiversity and represent a valuable source of new compounds. This study optimized the alkali-extraction conditions and antioxidant activities of soluble polysaccharides from the body wall of Sipunculus nudus. The effects of solid-liquid ratio, extraction duration, extraction temperature, and alkali concentration on the yield of S. nudus polysaccharides(SNP) were examined, according to which the optimal combination of extraction parameters was obtained by an orthogonal test. The relative influencing importance of different extraction parameters on the yield of SNP followed the order as solid-liquid ratio > extraction temperature > alkali concentration > extraction duration. The highest extraction yield, 1.98%, was achieved under an optimal extraction condition: temperature 60℃, solid-liquid ratio 1:6 g mL^(-1), duration 5 h, and alkali(Na OH) mass fraction 6%. The in vitro antioxidant activities examination showed that extracted SNP under this optimized condition had strong power in reducing certain hydroxyl and superoxide radical scavenging abilities. The promising results showed that extracted SNP could be a potent natural antioxidant.

Extraction Process and Antioxidant Activity of Polysaccharides from Cyathula Capitata( Wall. ) Moq.

[Objectives]To optimize the ultrasonic-assisted extraction process of polysaccharides from Cyathula Capitata( Wall.) Moq. and evaluate its antioxidant capacity in vitro. [Methods] The single factor orthogonal design was applied to explore the effects of ultrasonic time,solid-to-liquid ratio,extraction time and ethanol precipitation concentration on the extraction rate of crude polysaccharides from C. Capitata,to obtain the optimal extraction process; DPPH and ABTS^+ free radical scavenging ability was detected to evaluate its in vitro antioxidant activity.[Results]The optimal extraction process of C. Capitata crude polysaccharide was as follows: solid-to-liquid ratio was 40∶ 1,the extraction temperature was 50℃,the ultrasonic time was 80 min. Under such conditions,the extraction rate of C. Capitata crude polysaccharide was 10. 51%,and the purity was 56. 9%; C. Capitata crude polysaccharide has good scavenging ability to DPPH and ABTS^+ free radicals,and its IC_(50) values are 0. 468 and 0. 231 mg/mL,respectively. [Conclusions]The optimized process is simple and feasible,and the C. Capitata polysaccharide has good antioxidant activity in vitro. This experiment provides a basis for the further development and utilization of the C. Capitata polysaccharide.

Evaluation of the Antioxidant Activity of Ethanolic Extracts of Some Varieties of Onion

The content of polyphenolic in commercial onions has been determined. The antioxidant activity of their ethanolic extracts, as well as their effects on the oxidation of edible corn oil during accelerated ageing, was studied. Maceration of taxonomically identified commercial vegetable samples, previously peeled and thinly sliced, was carried out at ambient temperature, out of direct light, with occasional agitation and ultrasound, employing 95% ethyl alcohol as the extraction solvent, allowing them to stand for 7 days. The total polyphenolic contents were determined on the filtrated extracts using the Folin-Ciocalteau method. The antioxidant activity was evaluated on emulsions of ethanolic extracts of onion prepared in edible commercial corn oil, using sorbitan monooleate as emulsifying agent. The peroxide values were analyzed using the iodometric method;oxidation induction times were obtained from the peroxide evolution graphs, using the tangent method. Oil samples emulsified with ethanolic onion extracts showed an extension of the induction period. A 7 day ageing study at 45℃ was additionally performed to determine the conjugated dienes on pure commercial corn oil and its emulsions by visible spectrophotometry. The spectral analysis showed an increase of the measured absorbances in oil samples without additives and no change for the oils emulsified with onion extract. An increasing of diene values was observed for corn oil without additives during ageing;no changes in the value were observed in oils emulsified with onion extracts.

Evaluation of the Antioxidant Activity of Ethanolic Extracts of Some Varieties of Onions

The content of polyphenolic substances in commercial onions has been determined. The antioxidant activity of their ethanolic extracts, as well as their effects on the oxidation of edible corn oil during accelerated ageing was studied. Maceration of taxonomically identified commercial vegetable samples, previously peeled and thinly sliced, was carried out at ambient temperature, out of direct light, with occasional agitation and ultrasound, employing 95% ethyl alcohol as the extraction solvent, allowing them to stand for 7 days. The total polyphenolic contents were determined on the filtrated extracts using the Folin-Ciocalteau method. The antioxidant activity was evaluated on emulsions of ethanolic extracts of onion prepared in edible commercial corn oil, using sorbitan monooleate as emulsifying agent. The peroxide values were analyzed using the iodometric method;oxidation induction times were obtained from the peroxide evolution graphs, using the tan- gent method. Oil samples emulsified with ethanolic onion extracts showed an extension of the induction period. A 7-day ageing study at 45?C was additionally performed to determine the conjugated dienes on pure commercial corn oil and its emulsions by visible spectrophotometry. The spectral analysis showed an increase of the measured absorbancies in oil samples without additives and no change for the oils emulsified with onion extract. An increasing of diene values was observed for corn oil without additives during ageing;no changes in the value were observed in oils emulsified with onion extracts.

Ultrasound extraction optimization, structural features, andantioxidant activity of polysaccharides from Tricholoma matsutake

An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholoma matsutake fruiting bodies. Single-factor tests and orthogonal experimental design(L_9(3~3)) were used to obtain the optimal extraction conditions. Results showed that the optimal parameters were as follows: ultrasonic temperature, 40 °C; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 k Hz; and ultrasonic power, 100 W. Three novel T. matsutake polysaccharide(TMP) fractions(TMP30, TMP60, and TMP80) were isolated and purified from TMP by stepwise alcohol precipitation. Their preliminary structural features were determined by highperformance anion-exchange chromatography with pulsed-amperometric detection(HPAEC-PAD) and Fourier transform infrared spectrophotometer(FT-IR) analyses. Furthermore, their in vitro antioxidant activity was investigated in terms of a reducing power assay and the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl(DPPH) and hydroxyl radicals. The order of the various fractions based on their antioxidant activity was TMP80>TMP>TMP60>TMP30. These findings suggested that novel polysaccharide fractions from T. matsutake, especially TMP80, could be promising active macromolecules for biomedical use.

多花黄精多糖提取条件优化及其抗氧化能力评价

以多花黄精为主要原料,采用超声波提取法提取多花黄精多糖,选择微波功率、固液比、微波时间为单因素进行分析,采用正交实验优化多花黄精多糖提取条件;利用铁离子还原能力、DPPH·清除能力、ABTS^(+)·清除能力综合分析多花黄精多糖的体外抗氧化能力。研究结果显示,当微波功率为350 W、固液比为1∶20、微波时间为10 min时,多花黄精多糖的提取率为8.85%,效果最优。在此条件下提取多花黄精多糖,进行抗氧化能力评价,其铁离子还原能力为(59.35±0.73)μmol FE/g dw;DPPH·清除能力为(63.24±2.31)%;ABTS^(+)·清除能力为(47.58±1.49)%。总体可达到多花黄精多糖含量为3 mg/mL的水平。研究结果表明,在最优条件下提取多花黄精多糖提取率高,适合作为多花黄精深加工食品开发手段。

植物多糖的提取及生物学功能研究进展

植物多糖是由植物单糖聚合脱水形成的高分子聚合物,广泛分布于植物的各个器官中,具有抗肿瘤、抗菌、抗氧化、降血糖血脂等生物活性。不同提取方式提取的植物多糖分子量和分子形态不同,生物活性也有所差异。本文综述了目前研究较多的植物多糖提取方法,分析了植物多糖的主要生物活性,并总结了植物多糖在食品方面的应用,为植物多糖在食品领域的开发利用提供参考。

杨树桑黄与紫孢侧耳共培养胞内多糖提取工艺优化及抗氧化活性分析

本研究旨在探究超声波协同半仿生法提取杨树桑黄与紫孢侧耳液体共培养胞内多糖(CC-IPS)的工艺。通过单因素实验、Plackett-Burman设计和响应面设计试验,探究第1、2、3次的提取料液比、提取时间、提取温度和提取液pH这12个因素对CC-IPS提取量的影响,以DPPH和ABTS^(+)自由基清除率评价CC-IPS的体外抗氧化能力。结果表明:最佳提取工艺参数为第1、2、3次提取料液比分别为1:17、1:40、1:30(g/mL),第1、2、3次提取时间分别为31、30、30 min,第1、2、3次提取温度分别为44、40、50℃,第1、2、3次提取液pH分别为3、8、10。在此最优工艺条件下,CC-IPS提取量为86.4921±1.1924 mg/g,与预测值85.3981 mg/g接近。CCIPS对DPPH和ABTS^(+)自由基清除率的IC_(50)分别为0.2425和0.1376 mg/mL。该研究为真菌液体共培养胞内多糖的综合高效利用开发提供理论基础。

基于Box-Behnken设计优化毛菊苣药渣粗多糖酶法提取工艺

为了实现新疆特色植物药毛菊苣的药渣中有效成分毛菊苣粗多糖的高效提取与合理利用,采用响应面法优化了毛菊苣药渣粗多糖提取工艺条件,对提取得到的毛菊苣粗多糖运用液相色谱示差折光检测法(HPLC-RID)鉴定果糖和葡萄糖的含量,最后考察其体外抗氧化活性。以毛菊苣根醇提后的药渣为原料,毛菊苣粗多糖得率为考察指标,提取温度、提取时间、料液比及酶量为主要影响因素,在单因素试验的基础上,采用响应面法对毛菊苣药渣粗多糖提取工艺进行优化,运用国标检测方法HPLC-RID鉴定毛菊苣粗多糖中果糖和葡萄糖两种单糖的含量,通过1,1-二苯基-2-苦基肼自由基(1,1-diphenyl-2-picryl-hydrazylradical,DPPH)和2,2’-联氮-双-3-乙基苯并噻唑林-6-磺硫(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid),ABTS)对毛菊苣粗多糖的体外抗氧化活性进行考察。响应面试验法对毛菊苣药渣粗多糖进行优化的最优方案是提取时间2.0h,酶量2.0%,料液比1∶40(g·mL^(-1)),提取温度51℃,毛菊苣药渣使用该条件进行提取多糖的得率为(20.64±0.34)%,HPLC-RID法在乙腈∶水(75∶25,V∶V)为流动相,流速1.0mL·min^(-1),柱温35℃条件下能够实现高效分离鉴定,体外抗氧化试验表明毛菊苣粗多糖对DPPH自由基和ABTS自由基都有较好的清除作用。本研究获得了毛菊苣药渣粗多糖酶法的最佳提取工艺,并鉴定出毛菊苣粗多糖中果糖和葡萄糖两种单糖成分,且证明了毛菊苣药渣粗多糖具有良好的体外抗氧化活性。

鲍鱼脏器碱提多糖的分离纯化、结构表征及抗氧化活性研究

目的:分离纯化鲍鱼脏器碱提多糖(alkali-extracted abalone vscera polysaccharides,Aavp),并研究其结构和抗氧化活性,以期为鲍鱼脏器碱提多糖的开发及应用提供参考。方法:通过热碱提醇沉获得鲍鱼脏器碱提粗多糖,经DEAE Sepharose Fast Flow和Sephacryl S-400 HR纯化得到高纯度多糖,并利用气相色谱、高效液相色谱、红外光谱和热重分析仪等工具分析其结构及抗氧化活性。结果:粗多糖纯化后得到四个组分,包括Aavp Ia、Aavp Ib、Aavp IIa和Aavp IIb,其中Aavp IIa得率较高,选择其进一步进行结构分析。分析发现,Aavp IIa由木糖和半乳糖组成,分子量为166513 Da;糖苷键构型为α型,组成如下:半乳糖1→4糖苷键摩尔百分比为11.81%,半乳糖1→3糖苷键为34.14%,半乳糖1→2糖苷键为10.14%;木糖1→3糖苷键摩尔百分比为33.85%,1→2和1→4糖苷键共占10.06%。热重分析显示,Aavp IIa在226.4-332.6℃下断裂分解,热失重率为43.65%。抗氧化实验显示Aavp IIa对O2-·的清除率为85.89%,对DPPH·的清除率为62.17%,具有一定的抗氧化活性。结论:提取的鲍鱼脏器碱提多糖是一类杂多糖,并具有一定的抗氧化活性。

超声波-微波联合辅助提取澳洲坚果青皮多糖工艺优化及其抗氧化活性

为了开发澳洲坚果青皮多糖产品,促进澳洲坚果青皮的综合利用,我们以澳洲坚果青皮为试材,比较澳洲坚果果实不同部位的多糖提取率,并采用超声波-微波联合辅助法,运用单因素与正交试验比较微波功率、提取时间、料液比、超声温度4个因素对多糖提取率的影响,研究其最优提取条件。以维生素C为对照,测定澳洲坚果青皮多糖清除1,1-二苯基-2-三硝基苯肼(DPPH)、羟基自由基的能力与还原力,评价其抗氧化活性。结果表明,澳洲坚果果实不同部位中,青皮多糖提取率最高。青皮多糖超声波-微波联合辅助提取工艺中各因素对提取率的影响力从高到低依次为提取时间>料液比>超声温度>微波功率,且均达到极显著水平(p<0.01),最佳提取工艺条件为提取时间90 min、超声温度65℃、料液比(1∶25)g/mL、微波功率400 W,此条件下提取率达到1.96%。澳洲坚果青皮多糖的抗氧化活性与质量浓度呈极显著正相关(p<0.01),相关系数R分别为0.9707、0.9813、0.9912,具有较强的DPPH、羟基自由基清除能力和较高的还原力,其半抑制浓度(IC 50)分别为38.55、20.38、41.51μg/mL,相同浓度下略低于维生素C。