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Real-Time Detection and Instance Segmentation of Strawberry in Unstructured Environment
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作者 Chengjun Wang Fan Ding +4 位作者 Yiwen Wang Renyuan Wu Xingyu Yao Chengjie Jiang Liuyi Ling 《Computers, Materials & Continua》 SCIE EI 2024年第1期1481-1501,共21页
The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-r... The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-ronment is a challenging task.Current instance segmentation algorithms for strawberries suffer from issues such as poor real-time performance and low accuracy.To this end,the present study proposes an Efficient YOLACT(E-YOLACT)algorithm for strawberry detection and segmentation based on the YOLACT framework.The key enhancements of the E-YOLACT encompass the development of a lightweight attention mechanism,pyramid squeeze shuffle attention(PSSA),for efficient feature extraction.Additionally,an attention-guided context-feature pyramid network(AC-FPN)is employed instead of FPN to optimize the architecture’s performance.Furthermore,a feature-enhanced model(FEM)is introduced to enhance the prediction head’s capabilities,while efficient fast non-maximum suppression(EF-NMS)is devised to improve non-maximum suppression.The experimental results demonstrate that the E-YOLACT achieves a Box-mAP and Mask-mAP of 77.9 and 76.6,respectively,on the custom dataset.Moreover,it exhibits an impressive category accuracy of 93.5%.Notably,the E-YOLACT also demonstrates a remarkable real-time detection capability with a speed of 34.8 FPS.The method proposed in this article presents an efficient approach for the vision system of a strawberry-picking robot. 展开更多
关键词 YOLACT real-time detection instance segmentation attention mechanism STRAWBERRY
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NFA:A neural factorization autoencoder based online telephony fraud detection
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作者 Abdul Wahid Mounira Msahli +1 位作者 Albert Bifet Gerard Memmi 《Digital Communications and Networks》 SCIE CSCD 2024年第1期158-167,共10页
The proliferation of internet communication channels has increased telecom fraud,causing billions of euros in losses for customers and the industry each year.Fraudsters constantly find new ways to engage in illegal ac... The proliferation of internet communication channels has increased telecom fraud,causing billions of euros in losses for customers and the industry each year.Fraudsters constantly find new ways to engage in illegal activity on the network.To reduce these losses,a new fraud detection approach is required.Telecom fraud detection involves identifying a small number of fraudulent calls from a vast amount of call traffic.Developing an effective strategy to combat fraud has become challenging.Although much effort has been made to detect fraud,most existing methods are designed for batch processing,not real-time detection.To solve this problem,we propose an online fraud detection model using a Neural Factorization Autoencoder(NFA),which analyzes customer calling patterns to detect fraudulent calls.The model employs Neural Factorization Machines(NFM)and an Autoencoder(AE)to model calling patterns and a memory module to adapt to changing customer behaviour.We evaluate our approach on a large dataset of real-world call detail records and compare it with several state-of-the-art methods.Our results show that our approach outperforms the baselines,with an AUC of 91.06%,a TPR of 91.89%,an FPR of 14.76%,and an F1-score of 95.45%.These results demonstrate the effectiveness of our approach in detecting fraud in real-time and suggest that it can be a valuable tool for preventing fraud in telecommunications networks. 展开更多
关键词 Telecom industry Streaming anomaly detection Fraud analysis Factorization machine real-time system Security
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Real-Time Object Detection and Face Recognition Application for the Visually Impaired
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作者 Karshiev Sanjar Soyoun Bang +1 位作者 SookheeRyue Heechul Jung 《Computers, Materials & Continua》 SCIE EI 2024年第6期3569-3583,共15页
The advancement of navigation systems for the visually impaired has significantly enhanced their mobility by mitigating the risk of encountering obstacles and guiding them along safe,navigable routes.Traditional appro... The advancement of navigation systems for the visually impaired has significantly enhanced their mobility by mitigating the risk of encountering obstacles and guiding them along safe,navigable routes.Traditional approaches primarily focus on broad applications such as wayfinding,obstacle detection,and fall prevention.However,there is a notable discrepancy in applying these technologies to more specific scenarios,like identifying distinct food crop types or recognizing faces.This study proposes a real-time application designed for visually impaired individuals,aiming to bridge this research-application gap.It introduces a system capable of detecting 20 different food crop types and recognizing faces with impressive accuracies of 83.27%and 95.64%,respectively.These results represent a significant contribution to the field of assistive technologies,providing visually impaired users with detailed and relevant information about their surroundings,thereby enhancing their mobility and ensuring their safety.Additionally,it addresses the vital aspects of social engagements,acknowledging the challenges faced by visually impaired individuals in recognizing acquaintances without auditory or tactile signals,and highlights recent developments in prototype systems aimed at assisting with face recognition tasks.This comprehensive approach not only promises enhanced navigational aids but also aims to enrich the social well-being and safety of visually impaired communities. 展开更多
关键词 Artificial intelligence deep learning real-time object detection application
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Online Fault Monitoring of On-Load Tap-Changer Based on Voiceprint Detection
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作者 Kitwa Henock Bondo 《Journal of Power and Energy Engineering》 2024年第3期48-59,共12页
The continuous operation of On-Load Tap-Changers (OLTC) is essential for maintaining stable voltage levels in power transmission and distribution systems. Timely fault detection in OLTC is essential for preventing maj... The continuous operation of On-Load Tap-Changers (OLTC) is essential for maintaining stable voltage levels in power transmission and distribution systems. Timely fault detection in OLTC is essential for preventing major failures and ensuring the reliability of the electrical grid. This research paper proposes an innovative approach that combines voiceprint detection using MATLAB analysis for online fault monitoring of OLTC. By leveraging advanced signal processing techniques and machine learning algorithms in MATLAB, the proposed method accurately detects faults in OLTC, providing real-time monitoring and proactive maintenance strategies. 展开更多
关键词 online Fault Monitoring OLTC On-Load Tap Change Voiceprint detection
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A CNN-Based Single-Stage Occlusion Real-Time Target Detection Method
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作者 Liang Liu Nan Yang +4 位作者 Saifei Liu Yuanyuan Cao Shuowen Tian Tiancheng Liu Xun Zhao 《Journal of Intelligent Learning Systems and Applications》 2024年第1期1-11,共11页
Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The m... Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The method adopts the overall design of backbone network, detection network and algorithmic parameter optimisation method, completes the model training on the self-constructed occlusion target dataset, and adopts the multi-scale perception method for target detection. The HNM algorithm is used to screen positive and negative samples during the training process, and the NMS algorithm is used to post-process the prediction results during the detection process to improve the detection efficiency. After experimental validation, the obtained model has the multi-class average predicted value (mAP) of the dataset. It has general advantages over traditional target detection methods. The detection time of a single target on FDDB dataset is 39 ms, which can meet the need of real-time target detection. In addition, the project team has successfully deployed the method into substations and put it into use in many places in Beijing, which is important for achieving the anomaly of occlusion target detection. 展开更多
关键词 real-time Mask Target CNN (Convolutional Neural Network) Single-Stage detection Multi-Scale Feature Perception
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Online Intrusion Detection Mechanism Based on Model Migration in Intelligent Pumped Storage Power Stations 被引量:1
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作者 Yue Zong Yuanlin Luo +5 位作者 Yuechao Wu Jiande Huang Bowen Yang Xiaoyu Kang Shumei Liu Yao Yu 《China Communications》 SCIE CSCD 2023年第4期368-381,共14页
With the continuous integration of new energy into the power grid,various new attacks continue to emerge and the feature distributions are constantly changing during the deployment of intelligent pumped storage power ... With the continuous integration of new energy into the power grid,various new attacks continue to emerge and the feature distributions are constantly changing during the deployment of intelligent pumped storage power stations.The intrusion detection model trained on the old data is hard to effectively identify new attacks,and it is difficult to update the intrusion detection model in time when lacking data.To solve this issue,by using model-based transfer learning methods,in this paper we propose a convolutional neural network(CNN)based transfer online sequential extreme learning machine(TOS-ELM)scheme to enable the online intrusion detection,which is called CNN-TOSELM in this paper.In our proposed scheme,we use pre-trained CNN to extract the characteristics of the target domain data as input,and then build online learning classifier TOS-ELM to transfer the parameter of the ELM classifier of the source domain.Experimental results show the proposed CNNTOSELM scheme can achieve better detection performance and extremely short model update time for intelligent pumped storage power stations. 展开更多
关键词 transfer learning intrusion detection online classification
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Force Sensitive Resistors-Based Real-Time Posture Detection System Using Machine Learning Algorithms
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作者 Arsal Javaid Areeb Abbas +4 位作者 Jehangir Arshad Mohammad Khalid Imam Rahmani Sohaib Tahir Chauhdary Mujtaba Hussain Jaffery Abdulbasid S.Banga 《Computers, Materials & Continua》 SCIE EI 2023年第11期1795-1814,共20页
To detect the improper sitting posture of a person sitting on a chair,a posture detection system using machine learning classification has been proposed in this work.The addressed problem correlates to the third Susta... To detect the improper sitting posture of a person sitting on a chair,a posture detection system using machine learning classification has been proposed in this work.The addressed problem correlates to the third Sustainable Development Goal(SDG),ensuring healthy lives and promoting well-being for all ages,as specified by the World Health Organization(WHO).An improper sitting position can be fatal if one sits for a long time in the wrong position,and it can be dangerous for ulcers and lower spine discomfort.This novel study includes a practical implementation of a cushion consisting of a grid of 3×3 force-sensitive resistors(FSR)embedded to read the pressure of the person sitting on it.Additionally,the Body Mass Index(BMI)has been included to increase the resilience of the system across individual physical variances and to identify the incorrect postures(backward,front,left,and right-leaning)based on the five machine learning algorithms:ensemble boosted trees,ensemble bagged trees,ensemble subspace K-Nearest Neighbors(KNN),ensemble subspace discriminant,and ensemble RUSBoosted trees.The proposed arrangement is novel as existing works have only provided simulations without practical implementation,whereas we have implemented the proposed design in Simulink.The results validate the proposed sensor placements,and the machine learning(ML)model reaches a maximum accuracy of 99.99%,which considerably outperforms the existing works.The proposed concept is valuable as it makes it easier for people in workplaces or even at individual household levels to work for long periods without suffering from severe harmful effects from poor posture. 展开更多
关键词 Posture detection FSR sensor machine learning real-time KNN
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Towards Cache-Assisted Hierarchical Detection for Real-Time Health Data Monitoring in IoHT
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作者 Muhammad Tahir Mingchu Li +4 位作者 Irfan Khan Salman AAl Qahtani Rubia Fatima Javed Ali Khan Muhammad Shahid Anwar 《Computers, Materials & Continua》 SCIE EI 2023年第11期2529-2544,共16页
Real-time health data monitoring is pivotal for bolstering road services’safety,intelligence,and efficiency within the Internet of Health Things(IoHT)framework.Yet,delays in data retrieval can markedly hinder the eff... Real-time health data monitoring is pivotal for bolstering road services’safety,intelligence,and efficiency within the Internet of Health Things(IoHT)framework.Yet,delays in data retrieval can markedly hinder the efficacy of big data awareness detection systems.We advocate for a collaborative caching approach involving edge devices and cloud networks to combat this.This strategy is devised to streamline the data retrieval path,subsequently diminishing network strain.Crafting an adept cache processing scheme poses its own set of challenges,especially given the transient nature of monitoring data and the imperative for swift data transmission,intertwined with resource allocation tactics.This paper unveils a novel mobile healthcare solution that harnesses the power of our collaborative caching approach,facilitating nuanced health monitoring via edge devices.The system capitalizes on cloud computing for intricate health data analytics,especially in pinpointing health anomalies.Given the dynamic locational shifts and possible connection disruptions,we have architected a hierarchical detection system,particularly during crises.This system caches data efficiently and incorporates a detection utility to assess data freshness and potential lag in response times.Furthermore,we introduce the Cache-Assisted Real-Time Detection(CARD)model,crafted to optimize utility.Addressing the inherent complexity of the NP-hard CARD model,we have championed a greedy algorithm as a solution.Simulations reveal that our collaborative caching technique markedly elevates the Cache Hit Ratio(CHR)and data freshness,outshining its contemporaneous benchmark algorithms.The empirical results underscore the strength and efficiency of our innovative IoHT-based health monitoring solution.To encapsulate,this paper tackles the nuances of real-time health data monitoring in the IoHT landscape,presenting a joint edge-cloud caching strategy paired with a hierarchical detection system.Our methodology yields enhanced cache efficiency and data freshness.The corroborative numerical data accentuates the feasibility and relevance of our model,casting a beacon for the future trajectory of real-time health data monitoring systems. 展开更多
关键词 real-time health data monitoring Cache-Assisted real-time detection(CARD) edge-cloud collaborative caching scheme hierarchical detection Internet of Health Things(IoHT)
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LDA-ID:An LDA-Based Framework for Real-Time Network Intrusion Detection
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作者 Weidong Zhou Shengwei Lei +1 位作者 Chunhe Xia Tianbo Wang 《China Communications》 SCIE CSCD 2023年第12期166-181,共16页
Network intrusion poses a severe threat to the Internet.However,existing intrusion detection models cannot effectively distinguish different intrusions with high-degree feature overlap.In addition,efficient real-time ... Network intrusion poses a severe threat to the Internet.However,existing intrusion detection models cannot effectively distinguish different intrusions with high-degree feature overlap.In addition,efficient real-time detection is an urgent problem.To address the two above problems,we propose a Latent Dirichlet Allocation topic model-based framework for real-time network Intrusion Detection(LDA-ID),consisting of static and online LDA-ID.The problem of feature overlap is transformed into static LDA-ID topic number optimization and topic selection.Thus,the detection is based on the latent topic features.To achieve efficient real-time detection,we design an online computing mode for static LDA-ID,in which a parameter iteration method based on momentum is proposed to balance the contribution of prior knowledge and new information.Furthermore,we design two matching mechanisms to accommodate the static and online LDA-ID,respectively.Experimental results on the public NSL-KDD and UNSW-NB15 datasets show that our framework gets higher accuracy than the others. 展开更多
关键词 feature overlap LDA-ID optimal topic number determination real-time intrusion detection
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Portable FBAR based E-nose for cold chain real-time bananas shelf time detection
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作者 Chen Wu Jiuyan Li 《Nanotechnology and Precision Engineering》 CAS CSCD 2023年第1期32-39,共8页
Being cheap,nondestructive,and easy to use,gas sensors play important roles in the food industry.However,most gas sensors are suitable more for laboratory-quality fast testing rather than for cold-chain continuous and... Being cheap,nondestructive,and easy to use,gas sensors play important roles in the food industry.However,most gas sensors are suitable more for laboratory-quality fast testing rather than for cold-chain continuous and cumulative testing.Also,an ideal electronic nose(E-nose)in a cold chain should be stable to its surroundings and remain highly accurate and portable.In this work,a portable film bulk acoustic resonator(FBAR)-based E-nose was built for real-time measurement of banana shelf time.The sensor chamber to contain the portable circuit of the E-nose is as small as a smartphone,and by introducing an air-tight FBAR as a reference,the E-nose can avoid most of the drift caused by surroundings.With the help of porous layer by layer(LBL)coating of the FBAR,the sensitivity of the E-nose is 5 ppm to ethylene and 0.5 ppm to isoamyl acetate and isoamyl butyrate,while the detection range is large enough to cover a relative humidity of 0.8.In this regard,the E-nose can easily discriminate between yellow bananas with green necks and entirely yellow bananas while allowing the bananas to maintain their biological activities in their normal storage state,thereby showing the possibility of real-time shelf time detection.This portable FBAR-based E-nose has a large testing scale,high sensitivity,good humidity tolerance,and low frequency drift to its surroundings,thereby meeting the needs of cold-chain usage. 展开更多
关键词 Film bulk acoustic resonator(FBAR) Portable E-nose real-time detection Layer by layer
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Online Fault Detection Configuration on Equipment Side of a Variable-Air-Volume Air Handling Unit
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作者 杨学宾 李鑫海 +2 位作者 杨思钰 王吉 罗雯军 《Journal of Donghua University(English Edition)》 CAS 2023年第2期225-231,共7页
With the development of the technology of the Internet of Things,more and more operational data can be collected from air conditioning systems.Unfortunately,the most of existing air conditioning controllers mainly pro... With the development of the technology of the Internet of Things,more and more operational data can be collected from air conditioning systems.Unfortunately,the most of existing air conditioning controllers mainly provide controlling functions more than storing,processing or computing the measured data.This study develops an online fault detection configuration on the equipment side of air conditioning systems to realize these functions.Modbus communication is served to collect real-time operational data.The calculating programs are embedded to identify whether the measured signals exceed their limits or not,and to detect if sensor reading is frozen and other faults in relation to the operational performance are generated or not.The online fault detection configuration is tested on an actual variable-air-volume(VAV)air handling unit(AHU).The results show that the time ratio of fault detection exceeds 95.00%,which means that the configuration exhibits an acceptable fault detection effect. 展开更多
关键词 fault detection software configuration online monitoring equipment side variable-air-volume(VAV) air handling unit(AHU)
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Design of Online Vision Detection System for Stator Winding Coil
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作者 李艳 李芮 徐洋 《Journal of Donghua University(English Edition)》 CAS 2023年第6期639-648,共10页
The quality of the stator winding coil directly affects the performance of the motor.A dual-camera online machine vision detection method to detect whether the coil leads and winding regions were qualified was designe... The quality of the stator winding coil directly affects the performance of the motor.A dual-camera online machine vision detection method to detect whether the coil leads and winding regions were qualified was designed.A vision detection platform was designed to capture individual winding images,and an image processing algorithm was used for image pre-processing,template matching and positioning of the coil lead area to set up a coordinate system.After eliminating image noise by Blob analysis,the improved Canny algorithm was used to detect the location of the coil lead paint stripped region,and the time was reduced by about half compared to the Canny algorithm.The coil winding region was trained with the ShuffleNet V2-YOLOv5s model for the dataset,and the detect file was converted to the Open Neural Network Exchange(ONNX)model for the detection of winding cross features with an average accuracy of 99.0%.The software interface of the detection system was designed to perform qualified discrimination tests on the workpieces,and the detection data were recorded and statistically analyzed.The results showed that the stator winding coil qualified discrimination accuracy reached 96.2%,and the average detection time of a single workpiece was about 300 ms,while YOLOv5s took less than 30 ms. 展开更多
关键词 machine vision online detection V2-YOLOv5s model Canny algorithm stator winding coil
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Real-Time Spammers Detection Based on Metadata Features with Machine Learning
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作者 Adnan Ali Jinlong Li +2 位作者 Huanhuan Chen Uzair Aslam Bhatti Asad Khan 《Intelligent Automation & Soft Computing》 2023年第12期241-258,共18页
Spammer detection is to identify and block malicious activities performing users.Such users should be identified and terminated from social media to keep the social media process organic and to maintain the integrity ... Spammer detection is to identify and block malicious activities performing users.Such users should be identified and terminated from social media to keep the social media process organic and to maintain the integrity of online social spaces.Previous research aimed to find spammers based on hybrid approaches of graph mining,posted content,and metadata,using small and manually labeled datasets.However,such hybrid approaches are unscalable,not robust,particular dataset dependent,and require numerous parameters,complex graphs,and natural language processing(NLP)resources to make decisions,which makes spammer detection impractical for real-time detection.For example,graph mining requires neighbors’information,posted content-based approaches require multiple tweets from user profiles,then NLP resources to make decisions that are not applicable in a real-time environment.To fill the gap,firstly,we propose a REal-time Metadata based Spammer detection(REMS)model based on only metadata features to identify spammers,which takes the least number of parameters and provides adequate results.REMS is a scalable and robust model that uses only 19 metadata features of Twitter users to induce 73.81%F1-Score classification accuracy using a balanced training dataset(50%spam and 50%genuine users).The 19 features are 8 original and 11 derived features from the original features of Twitter users,identified with extensive experiments and analysis.Secondly,we present the largest and most diverse dataset of published research,comprising 211 K spam users and 1 million genuine users.The diversity of the dataset can be measured as it comprises users who posted 2.1 million Tweets on seven topics(100 hashtags)from 6 different geographical locations.The REMS’s superior classification performance with multiple machine and deep learning methods indicates that only metadata features have the potential to identify spammers rather than focusing on volatile posted content and complex graph structures.Dataset and REMS’s codes are available on GitHub(www.github.com/mhadnanali/REMS). 展开更多
关键词 Spam detection online social networks METADATA machine learning
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Cyber Resilience through Real-Time Threat Analysis in Information Security
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作者 Aparna Gadhi Ragha Madhavi Gondu +1 位作者 Hitendra Chaudhary Olatunde Abiona 《International Journal of Communications, Network and System Sciences》 2024年第4期51-67,共17页
This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends t... This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends that they are not the same. The concept of cyber security is explored, which goes beyond protecting information resources to include a wider variety of assets, including people [1]. Protecting information assets is the main goal of traditional information security, with consideration to the human element and how people fit into the security process. On the other hand, cyber security adds a new level of complexity, as people might unintentionally contribute to or become targets of cyberattacks. This aspect presents moral questions since it is becoming more widely accepted that society has a duty to protect weaker members of society, including children [1]. The study emphasizes how important cyber security is on a larger scale, with many countries creating plans and laws to counteract cyberattacks. Nevertheless, a lot of these sources frequently neglect to define the differences or the relationship between information security and cyber security [1]. The paper focus on differentiating between cybersecurity and information security on a larger scale. The study also highlights other areas of cybersecurity which includes defending people, social norms, and vital infrastructure from threats that arise from online in addition to information and technology protection. It contends that ethical issues and the human factor are becoming more and more important in protecting assets in the digital age, and that cyber security is a paradigm shift in this regard [1]. 展开更多
关键词 Cybersecurity Information Security Network Security Cyber Resilience real-time Threat Analysis Cyber Threats Cyberattacks Threat Intelligence Machine Learning Artificial Intelligence Threat detection Threat Mitigation Risk Assessment Vulnerability Management Incident Response Security Orchestration Automation Threat Landscape Cyber-Physical Systems Critical Infrastructure Data Protection Privacy Compliance Regulations Policy Ethics CYBERCRIME Threat Actors Threat Modeling Security Architecture
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Real-Time Detection of Cracks on Concrete Bridge Decks Using Deep Learning in the Frequency Domain 被引量:9
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作者 Qianyun Zhang Kaveh Barri +1 位作者 Saeed K.Babanajad Amir H.Alavi 《Engineering》 SCIE EI 2021年第12期1786-1796,共11页
This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequen... This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequency domain.The so-called 1D-CNN-LSTM algorithm is trained using thousands of images of cracked and non-cracked concrete bridge decks.In order to improve the training efficiency,images are first transformed into the frequency domain during a preprocessing phase.The algorithm is then calibrated using the flattened frequency data.LSTM is used to improve the performance of the developed network for long sequence data.The accuracy of the developed model is 99.05%,98.9%,and 99.25%,respectively,for training,validation,and testing data.An implementation framework is further developed for future application of the trained model for large-scale images.The proposed 1D-CNN-LSTM method exhibits superior performance in comparison with existing deep learning methods in terms of accuracy and computation time.The fast implementation of the 1D-CNN-LSTM algorithm makes it a promising tool for real-time crack detection. 展开更多
关键词 Crack detection Concrete bridge deck Deep learning real-time
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A fast and adaptive method for automatic weld defect detection in various real-time X-ray imaging systems 被引量:10
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作者 邵家鑫 都东 +2 位作者 石涵 常保华 郭桂林 《China Welding》 EI CAS 2012年第1期8-12,共5页
A first and effective method is proposed to detect weld deject adaptively in various Dypes of real-time X-ray images obtained in different conditions. After weld extraction and noise reduction, a proper template of me... A first and effective method is proposed to detect weld deject adaptively in various Dypes of real-time X-ray images obtained in different conditions. After weld extraction and noise reduction, a proper template of median filter is used to estimate the weld background. After the weld background is subtracted from the original image, an adaptite threshold segmentation algorithm is proposed to obtain the binary image, and then the morphological close and open operation, labeling algorithm and fids'e alarm eliminating algorithm are applied to pracess the binary image to obtain the defect, ct detection result. At last, a fast realization procedure jbr proposed method is developed. The proposed method is tested in real-time X-ray image,s obtairted in different X-ray imaging sutems. Experiment results show that the proposed method is effective to detect low contrast weld dejects with few .false alarms and is adaptive to various types of real-time X-ray imaging systems. 展开更多
关键词 non-destructive testing real-time X-ray imaging weld defect automatie detection
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Real-time Fluorescence PCR Method for Detection of Burkholderia glumae from Rice 被引量:5
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作者 FANG Yuan XU Li-hui TIAN Wen-xiao HUAI Yan YU Shan-hong LOU Miao-miao XIE Guan-lin 《Rice science》 SCIE 2009年第2期157-160,共4页
Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further ... Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1:100. 展开更多
关键词 Burkholderia glumae bacterial grain rot detection real-time fluorescence polymerase chain reaction DCE
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Development and validation of a TaqMan^(TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda 被引量:2
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作者 XIE Guosi HUANG Jie +3 位作者 ZHANG Qingli HAN Nana SHI Chengyin WANG Xiuhua 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2012年第4期140-148,共9页
Edwardsiella tarda is one of the most important emerging pathogens in tile global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In t... Edwardsiella tarda is one of the most important emerging pathogens in tile global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In this study, primers and a TaqMan probe specific to the conservative sequences of the 16S rRNA gene of E. tarda were designed. The concentration of primers and TaqMan probe were optimized to 200 nmol/L and 120 nmol/L, respectively. The detection sensitivity of the FQ- PCR assay was determined to be as low as five copies of the target sequence per reaction using the pGEM-16S rDNA recombinant plasmid as a template, which was 100 times more sensitive than conventional PCR. A standard curve by plotting the threshold cycle values (y) against the common logarithmic copies (logl0n~ as x; n~ is copy number) of pGEM-16S rDNA was generated. The results of intra- and inter-assay variability tests demonstrate that the established FQ-PCR method was highly reproducible. The assay was specific for E. tarda as it showed that there was no cross-reactivity to eight additional bacterial pathogen strains in aquaculture. Thus, the FQ-PCR assay has the potential for diagnostic purposes and for other applications, especially for the rapid detection and quantification of low-grade E. tarda infections. 展开更多
关键词 Edwardsiella tarda TAQMAN real-time PCR detection 16S rDNA
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Real-time RT-PCR Assay for the detection of Tahyna Virus 被引量:2
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作者 LI Hao CAO Yu Xi +6 位作者 HE Xiao Xia FU Shi Hong LYU Zhi HE Ying GAO Xiao Yan LIANG Guo Dong WANG Huan Yu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第5期374-377,共4页
A real-time RT-PCR (RT-qPCR) assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequ... A real-time RT-PCR (RT-qPCR) assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequences encoding the nucleocapsid protein from the Tahyna virus. Primers and probes were selected in conserved regions by aligning genetic sequences from various Tahyna virus strains available from GenBank. The sensitivity of the RT-qPCR approach was compared to that of a standard plaque assay in BHK cells. RT-qPCR assay can detect 4.8 PFU of titrated Tahyna virus. Assay specificities were determined by testing a battery of arboviruses, including representative strains of Tahyna virus and other arthropod-borne viruses from China. Seven strains of Tahyna virus were confirmed as positive; the other seven species of arboviruses could not be detected by RT-qPCR. Additionally, the assay was used to detect Tahyna viral RNA in pooled mosquito samples. The RT-qPCR assay detected Tahyna virus in a sensitive, specific, and rapid manner; these findings support the use of the assay in viral surveillance. 展开更多
关键词 PCR real-time RT-PCR Assay for the detection of Tahyna Virus TIME RT
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Online volume rendering of incrementally accumulated LSCEM images for superficial oral cancer detection 被引量:2
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作者 Wei Ming Chiew Feng Lin +1 位作者 Kemao Qian Hock Soon Seah 《World Journal of Clinical Oncology》 CAS 2011年第4期179-186,共8页
Laser scanning confocal endomicroscope(LSCEM)has emerged as an imaging modality which provides noninvasive,in vivo imaging of biological tissue on a microscopic scale.Scientific visualizations for LSCEM datasets captu... Laser scanning confocal endomicroscope(LSCEM)has emerged as an imaging modality which provides noninvasive,in vivo imaging of biological tissue on a microscopic scale.Scientific visualizations for LSCEM datasets captured by current imaging systems require these datasets to be fully acquired and brought to a separate rendering machine.To extend the features and capabilities of this modality,we propose a system which is capable of performing realtime visualization of LSCEM datasets.Using field-programmable gate arrays,our system performs three tasks in parallel:(1)automated control of dataset acquisition;(2)imaging-rendering system synchronization;and(3)realtime volume rendering of dynamic datasets.Through fusion of LSCEM imaging and volume rendering processes,acquired datasets can be visualized in realtime to provide an immediate perception of the image quality and biological conditions of the subject,further assisting in realtime cancer diagnosis.Subsequently,the imaging procedure can be improved for more accurate diagnosis and reduce the need for repeating the process due to unsatisfactory datasets. 展开更多
关键词 CONFOCAL endomicroscope Field-programmable gate arrays Incrementally accumulated volume RENDERING REALTIME online cancer detection
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