To select a marine teleost fish which can be used as a bioindicator of cadmium （Cd） pollution in the Gulf of Gabes in Tunisia, Cd concentrations in liver and gill were compared in three benthic fish species includin...To select a marine teleost fish which can be used as a bioindicator of cadmium （Cd） pollution in the Gulf of Gabes in Tunisia, Cd concentrations in liver and gill were compared in three benthic fish species including Salaria basilisca, Zosterisessor ophiocephalus and Solea vulgaris. Fish samples were collected from three selected sites in the Gulf of Gabes, with different degrees of Cd contamination： the industrialized coast of Sfax （S 1）, the coast of Douar Chatt （S2） and the coast of Luza （S3）. The results shows that Cd concentrations in both sediment and water collected from S1 were significantly higher （t9 〈 0.0001） than those from S2 and S3. For each species, Cd concentrations, in both liver and gill, showed the decreasing order： S 1 〉 S2 〉 S3. The highest concentration of Cd was detected in the liver of S. basilisca, and only S. basilisca showed bioaccumulation factors （BAF） greater than 1 in all studied sites. In S 1 and S2, BAF values respect the following order： S. basilisca 〉 Z. ophiocephalus 〉 S. vulgaris. These results of significant bioaccumulation of Cd, in terms of hepatic concentrations and bioaccumulation factors, indicated that S. basilisca can be used as bioindicator to evaluate the evolution of Cd pollution in the Gulf of Gabes.展开更多
文摘以乌鳢(Ophiocephalus argus)为研究对象,采用不同日粮饲喂,试验W1组饲喂冰鲜鱼,试验W2组饲喂畜禽内脏。12周后,取肠道内容物,利用16S rDNA技术研究投喂不同饲粮的乌鳢肠道生物群落结构差异和微生物多样性,分别获得W1组和W2组乌鳢肠道细菌有效序列51124和39298条。结果分析表明;W1组和W2组物种注释(operational taxonomic unit,OTU)数目分别为2631和4114条,分类地位明确的细菌种类分别隶属5个属和49个属,W1组和W2组乌鳢肠道细菌种类差异很大。W1组乌鳢肠道细菌的优势类群是邻单胞菌属(Plesiomonas)、狭义梭菌属(Clostridium sensu stricto)、鲸蜡菌属(Cetobacterium);W2组乌鳢肠道细菌的优势类群是狭义梭菌属(Clostridium sensu stricto)、邻单胞菌属(Plesiomonas)、Spartobacteria、甲基副球菌属(Methyloparacoccus)、杆菌属(Alsobacter)、Saccharibacteria、分支杆菌属(Mycobacterium)。W2组乌鳢肠道细菌香农指数高于W1组乌鳢肠道细菌,表明前者肠道细菌多样性大于后者。本实验揭示了畜禽内脏饲喂乌鳢肠道细菌的群落种类多样性、OTU丰富度和物种总数均高于冰鲜鱼饲喂乌鳢;W1组肠道有益菌OTU丰富度大于W2组,且W2组还存在一些潜在致病菌。本研究为乌鳢的健康养殖和后续肠道微生物资源的挖掘提供了理论依据。
文摘To select a marine teleost fish which can be used as a bioindicator of cadmium （Cd） pollution in the Gulf of Gabes in Tunisia, Cd concentrations in liver and gill were compared in three benthic fish species including Salaria basilisca, Zosterisessor ophiocephalus and Solea vulgaris. Fish samples were collected from three selected sites in the Gulf of Gabes, with different degrees of Cd contamination： the industrialized coast of Sfax （S 1）, the coast of Douar Chatt （S2） and the coast of Luza （S3）. The results shows that Cd concentrations in both sediment and water collected from S1 were significantly higher （t9 〈 0.0001） than those from S2 and S3. For each species, Cd concentrations, in both liver and gill, showed the decreasing order： S 1 〉 S2 〉 S3. The highest concentration of Cd was detected in the liver of S. basilisca, and only S. basilisca showed bioaccumulation factors （BAF） greater than 1 in all studied sites. In S 1 and S2, BAF values respect the following order： S. basilisca 〉 Z. ophiocephalus 〉 S. vulgaris. These results of significant bioaccumulation of Cd, in terms of hepatic concentrations and bioaccumulation factors, indicated that S. basilisca can be used as bioindicator to evaluate the evolution of Cd pollution in the Gulf of Gabes.
