Optical barcodes have demonstrated a great potential in multiplexed bioassays and cell tracking for their distinctive spectral fingerprints.The vast majority of optical barcodes were designed to identify a specific ta...Optical barcodes have demonstrated a great potential in multiplexed bioassays and cell tracking for their distinctive spectral fingerprints.The vast majority of optical barcodes were designed to identify a specific target by fluorescence emission spectra,without being able to characterize dynamic changes in response to analytes through time.To overcome these limitations,the concept of the bioresponsive dynamic photonic barcode was proposed by exploiting interfacial energy transfer between a microdroplet cavity and binding molecules.Whispering-gallery modes resulting from cavity-enhanced energy transfer were therefore converted into photonic barcodes to identify binding activities,in which more than trillions of distinctive barcodes could be generated by a single droplet.Dynamic spectral barcoding was achieved by a significant improvement in terms of signal-to-noise ratio upon binding to target molecules.Theoretical studies and experiments were conducted to elucidate the effect of different cavity sizes and analyte concentrations.Timeresolved fluorescence lifetime was implemented to investigate the role of radiative and non-radiative energy transfer.Finally,microdroplet photonic barcodes were employed in biodetection to exhibit great potential in fulfilling biomedical applications.展开更多
The method for batch continuous production of polymerized cholesteric liquid crystal(PCLC) microdisks based on centrifugal microfluidics was proposed. The prototype centrifugal microfluidic chip was fabricated by the ...The method for batch continuous production of polymerized cholesteric liquid crystal(PCLC) microdisks based on centrifugal microfluidics was proposed. The prototype centrifugal microfluidic chip was fabricated by the wetetching technique with a series of ladder-like concentric ring channels whose depths decrease radially. Two types of dye-doped PCLC microdisks with different doping procedures were generated by this method, the tunable lasing properties were characterized, and corresponding potential applications in a micro-cavity laser and optical barcode were demonstrated. The method is also applicable to a broad range of other polymerized materials.展开更多
基金We would like to thank the Centre of Bio-Devices and Bioinformatics and CNRS International—Nanyang Technological University-Thales Research Alliance(CINTRA)for lab supportWe would also like to thank NTU for the startup grant(SUG-M4082308.040).
文摘Optical barcodes have demonstrated a great potential in multiplexed bioassays and cell tracking for their distinctive spectral fingerprints.The vast majority of optical barcodes were designed to identify a specific target by fluorescence emission spectra,without being able to characterize dynamic changes in response to analytes through time.To overcome these limitations,the concept of the bioresponsive dynamic photonic barcode was proposed by exploiting interfacial energy transfer between a microdroplet cavity and binding molecules.Whispering-gallery modes resulting from cavity-enhanced energy transfer were therefore converted into photonic barcodes to identify binding activities,in which more than trillions of distinctive barcodes could be generated by a single droplet.Dynamic spectral barcoding was achieved by a significant improvement in terms of signal-to-noise ratio upon binding to target molecules.Theoretical studies and experiments were conducted to elucidate the effect of different cavity sizes and analyte concentrations.Timeresolved fluorescence lifetime was implemented to investigate the role of radiative and non-radiative energy transfer.Finally,microdroplet photonic barcodes were employed in biodetection to exhibit great potential in fulfilling biomedical applications.
基金supported by the National Natural Science Foundation of China(NSFC)(No.61675172)the Natural Science Foundation of Fujian Province,China(No.2017J01124)。
文摘The method for batch continuous production of polymerized cholesteric liquid crystal(PCLC) microdisks based on centrifugal microfluidics was proposed. The prototype centrifugal microfluidic chip was fabricated by the wetetching technique with a series of ladder-like concentric ring channels whose depths decrease radially. Two types of dye-doped PCLC microdisks with different doping procedures were generated by this method, the tunable lasing properties were characterized, and corresponding potential applications in a micro-cavity laser and optical barcode were demonstrated. The method is also applicable to a broad range of other polymerized materials.
