Intravascular photoacoustic and optical coherence tomography imaging dual-mode system for detecting spontaneous coronary artery dissection: A feasibility study

In this work,we present an intravascular dual-mode endoscopic system capable of both intravascular photoacoustic imaging(IVPAI)and intravascular optical coherence tomography(IVOCT)for recognizing spontaneous coronary artery dissection(SCAD)phantoms.IVPAI provides high-resolution and high-penetration images of intramural hematoma(IMH)at different depths,so it is especially useful for imaging deep blood clots associated with imaging phantoms.IVOCT can readily visualize the double-lumen morphology of blood vessel walls to identify intimal tears.We also demonstrate the capability of this dual-mode endoscopic system using mimicking phantoms and biological samples of blood clots in ex vivo porcine arteries.The results of the experiments indicate that the combined IVPAI and IVOCT technique has the potential to provide a more accurate SCAD assessment method for clinical applications.

Experimental Study on the Variation of Optical Imaging Characteristics with Zenith Angle due to Internal Solitary Waves in Sunglint

Internal solitary waves(ISWs)change the roughness of the sea surface,thus producing dark and bright bands in optical images.However,reasons for changes in imaging characteristics with the solar zenith angle remain unclear.In this paper,the optical imaging pattern of ISWs in sunglint under different zenith angles of the light source is investigated by collecting optical images of ISWs through physical simulation.The experiment involves setting 10 zenith angles of the light source,which are divided into area a the optical images of ISWs in the three areas show dark-bright mode,single bright band,and bright-dark mode,which are consistent with those observed by optical remote sensing.In addition,this study analyzed the percentage of the dark and bright areas of the bands and the change in the relative gray difference and found changes in both areas under different zenith angles of the light source.The MODIS and ASAR images display a similar brightness-darkness distance of the same ISWs.Therefore,the relationship between the brightness-darkness distance and the characteristic half-width of ISWs is determined in accordance with the eKdV theory and the imaging mechanism of ISWs of the SAR image.Overall,the relationship between them in the experiment is almost consistent with the theoretical result.

MACS-W:A modified optical clearing agent for imaging 3D cell cultures

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

Deep learning-based inpainting of saturation artifacts in optical coherence tomography images

Limited by the dynamic range of the detector,saturation artifacts usually occur in optical coherence tomography(OCT)imaging for high scattering media.The available methods are difficult to remove saturation artifacts and restore texture completely in OCT images.We proposed a deep learning-based inpainting method of saturation artifacts in this paper.The generation mechanism of saturation artifacts was analyzed,and experimental and simulated datasets were built based on the mechanism.Enhanced super-resolution generative adversarial networks were trained by the clear–saturated phantom image pairs.The perfect reconstructed results of experimental zebrafish and thyroid OCT images proved its feasibility,strong generalization,and robustness.

The Application of Digital Optical 3D Image Analyzer Evaskin in The Evaluation of Wrinkles

To verify the effectiveness of digital optical 3D image analyzer EvaSKIN in the objective and quantitative evaluation of wrinkles.A total of 115 subjects were recruited,the facial images of the subjects were collected by digital optical 3D image analyzer and manual camera,the changes of crow’s feet with age were analyzed.Pictures obtained by manual photography can be directly used for observation and preliminary grading of wrinkles.However,the requirements for evaluators are high,and the results are prone to errors,which will affect the accuracy of the evaluation.Therefore,skilled raters are needed.Compared with the manual photography method,the digital optical 3D image analyzer EvaSKIN can realize three-dimensional extraction of wrinkles,and obtain the change trend of crow’s feet with age.20~30 years old,wrinkles begin to appear slowly;wrinkles will increase rapidly at the age of 30~50;The length of 50~60 year old wrinkles is basically fixed,the wrinkles develop longitudewise,gradually widen and deepen,and the area,depth and volume increase is obvious,and the skin aging condition is intensified.the digital optical 3D image analyzer EvaSKIN realizes the 3D extraction of wrinkles,quantifies the circumference,area,average depth,maximum depth and volume of wrinkles,realizes the objective and quantitative evaluation of wrinkle state,is more accurate in the measurement of wrinkles,and provides a new instrument and method for the evaluation of wrinkles.it is a perfect and supplement to the traditional evaluation methods,and to a certain extent,it helps the research and development and evaluation institutions of cosmetics to obtain more abundant and three-dimensional data support.

An optical tweezer-based microdroplet imaging technology

Microspheres can break the diffraction limit and magnify nano-structure imaging,and with its advantages of low cost and label-free operation,microsphere-assisted imaging has become an irreplaceable tool in the life sciences and for precision measurements.However,the tiny size and limited imaging field of traditional solid microspheres cause difficulties when imaging large sample areas.Alternatively,droplets have similar properties to those of microspheres,with large surface curvature and refractive-index difference from the surrounding environment,and they can also serve as lenses to focus light for observation and imaging.Previous work has shown that droplets with controllable size can be generated using an optical tweezer system and can be driven by optical traps to move precisely like solid microspheres.Here,a novel microdroplet-assisted imaging technology based on optical tweezers is proposed that better integrates the generation,manipulation,and utilization of droplets.

Optical encryption scheme based on spread spectrum ghost imaging

An optical encryption(OE) scheme based on the spread spectrum ghost imaging(SSGI), named as SSGI-OE, is proposed to obtain a high security with a smaller key. In the scheme, the randomly selected row number of a Hadamard matrix of order N is used as the secure key, and shared with the authorized user, Bob, through a private channel. Each corresponding row vector of the order-N Hadamard matrix is then used as the direct sequence code to modulate a speckle pattern for the ghost imaging system, and an image is encrypted with the help of the SSGI. The measurement results from the bucket detector, named as ciphertext, are then transmitted to Bob through a public channel. The illuminating speckle patterns are also shared with Bob by the public channel. With the correct secure key, Bob could reconstruct the image with the aid of the SSGI system, whereas the unauthorized user, Eve, could not obtain any useful information of the encrypted image. The numerical simulations and experimental results show that the proposed scheme is feasible with a higher security and a smaller key. For the 32 × 32 pixels image, the number of bits sent from Alice to Bob by using SSGIOE(M = 1024, N = 2048) scheme is only 0.0107 times over a computational ghost imaging optical encryption scheme.When the eavesdropping ratio(ER) is less than 40%, the eavesdropper cannot acquire any information of the encrypted image. The extreme circumstance for the proposed SSGI-OE scheme is also discussed, where the eavesdropper begins to extract the information when ER is up to 15%.

Real-time and high-transmission middle-infrared optical imaging system based on a pixel-wise metasurface micro-polarization array

Real-time polarization medium-wave infrared(MIR)optical imaging systems enable the acquisition of infrared and polarization information for a target.At present,real-time polarization MIR devices face the following problems:poor real-time performance,low transmission and high requirements for fabrication and integration.Herein,we aim to improve the performance of real-time polarization imaging systems in the MIR waveband and solve the above-mentioned defects.Therefore,we propose a MIR polarization imaging system to achieve real-time polarization-modulated imaging with high transmission as well as improved performance based on a pixel-wise metasurface micro-polarization array(PMMPA).The PMMPA element comprises several linear polarization(LP)filters with different polarization angles.The optimization results demonstrate that the transmittance of the center field of view for the LP filters is up to 77%at a wavelength of4.0μm and an extinction ratio of 88 d B.In addition,a near-diffraction-limited real-time MIR imaging optical system is designed with a field of view of 5°and an F-number of 2.The simulation results show that an MIR polarization imaging system with excellent real-time performance and high transmission is achieved by using the optimized PMMPA element.Therefore,the method is compatible with the available optical system design technologies and provides a way to realize real-time polarization imaging in MIR wavebands.

Research on the model of high robustness computational optical imaging system

Computational optical imaging is an interdisciplinary subject integrating optics, mathematics, and information technology. It introduces information processing into optical imaging and combines it with intelligent computing, subverting the imaging mechanism of traditional optical imaging which only relies on orderly information transmission. To meet the high-precision requirements of traditional optical imaging for optical processing and adjustment, as well as to solve its problems of being sensitive to gravity and temperature in use, we establish an optical imaging system model from the perspective of computational optical imaging and studies how to design and solve the imaging consistency problem of optical system under the influence of gravity, thermal effect, stress, and other external environment to build a high robustness optical system. The results show that the high robustness interval of the optical system exists and can effectively reduce the sensitivity of the optical system to the disturbance of each link, thus realizing the high robustness of optical imaging.

Wide-spectrum optical synthetic aperture imaging via spatial intensity interferometry

High resolution imaging is achieved using increasingly larger apertures and successively shorter wavelengths.Optical aperture synthesis is an important high-resolution imaging technology used in astronomy.Conventional long baseline amplitude interferometry is susceptible to uncontrollable phase fluctuations,and the technical difficulty increases rapidly as the wavelength decreases.The intensity interferometry inspired by HBT experiment is essentially insensitive to phase fluctuations,but suffers from a narrow spectral bandwidth which results in a lack of effective photons.In this study,we propose optical synthetic aperture imaging based on spatial intensity interferometry.This not only realizes diffraction-limited optical aperture synthesis in a single shot,but also enables imaging with a wide spectral bandwidth,which greatly improves the optical energy efficiency of intensity interferometry.And this method is insensitive to the optical path difference between the sub-apertures.Simulations and experiments present optical aperture synthesis diffraction-limited imaging through spatial intensity interferometry in a 100 nm spectral width of visible light,whose maximum optical path difference between the sub-apertures reaches 69λ.This technique is expected to provide a solution for optical aperture synthesis over kilometer-long baselines at optical wavelengths.

Observing single cells in whole organs with optical imaging

Cells are the basic unit of human organs that are not fully understood.The revolutionary advancements of optical imaging alowed us to observe single cells in whole organs,revealing the complicated composition of cells with spatial information.Therefore,in this review,we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals.Then,we describe optical imaging to achieve threedimensional spatial discrimination for biological tisutes.Due to the milky appearance of tissues,the spatial information burred deep in the whole organ.Fortunately,strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.

Optical coherence tomography enhanced depth imaging of chorioretinal folds in patients with orbital tumors

AIM:To characterize spectral-domain optical coherence tomography(SD-OCT)features of chorioretinal folds in orbital mass imaged using enhanced depth imaging(EDI).METHODS:Prospective observational case-control study was conducted in 20 eyes of 20 patients,the uninvolved eye served as a control.All the patients underwent clinical fundus photography,computed tomography,EDI SDOCT imaging before and after surgery.Two patients with cavernous hemangiomas underwent intratumoral injection of bleomycin A5;the remaining patients underwent tumor excision.Patients were followed 1 to 14mo following surgery(average follow up,5.8mo).RESULTS:Visual acuity prior to surgery ranged from 20/20 to 20/200.Following surgery,5 patients’visual acuity remained unchanged while the remaining 15 patients had a mean letter improvement of 10(range 4 to 26 letters).Photoreceptor inner/outer segment defects were found in 10 of 15 patients prior to surgery.Following surgical excision,photoreceptor inner/outer segment defects fully resolved in 8 of these 10 patients.CONCLUSION:Persistence of photoreceptor inner/outer segment defects caused by compression of the globe by an orbital mass can be associated with reduced visual prognosis.Our findings suggest that photoreceptor inner/outer segment defects on EDI SD-OCT could be an indicator for immediate surgical excision of an orbital mass causing choroidal compression.

Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters

BACKGROUND Radionuclides produce Cherenkov radiation(CR),which can potentially activate photosensitizers(PSs)in phototherapy.Several groups have studied Cherenkov energy transfer to PSs using optical imaging;however,cost-effectively identifying whether PSs are excited by radionuclide-derived CR and detecting fluorescence emission from excited PSs remain a challenge.Many laboratories face the need for expensive dedicated equipment.AIM To cost-effectively confirm whether PSs are excited by radionuclide-derived CR and distinguish fluorescence emission from excited PSs.METHODS The absorbance and fluorescence spectra of PSs were measured using a microplate reader and fluorescence spectrometer to examine the photo-physical properties of PSs.To mitigate the need for expensive dedicated equipment and achieve the aim of the study,we developed a method that utilizes a chargecoupled device optical imaging system and appropriate long-pass filters of different wavelengths(manual sequential application of long-pass filters of 515,580,645,700,750,and 800 nm).Tetrakis(4-carboxyphenyl)porphyrin(TCPP)was utilized as a model PS.Different doses of copper-64(^(64)CuCl_(2))(4,2,and 1 mCi)were used as CR-producing radionuclides.Imaging and data acquisition were performed 0.5 h after sample preparation.Differential image analysis was conducted by using ImageJ software(National Institutes of Health)to visually evaluate TCPP fluorescence.RESULTS The maximum absorbance of TCPP was at 390-430 nm,and the emission peak was at 670 nm.The CR and CRinduced TCPP emissions were observed using the optical imaging system and the high-transmittance long-pass filters described above.The emission spectra of TCPP with a peak in the 645-700 nm window were obtained by calculation and subtraction based on the serial signal intensity(total flux)difference between^(64)CuCl_(2)+TCPP and^(64)CuCl_(2).Moreover,the differential fluorescence images of TCPP were obtained by subtracting the^(64)CuCl_(2)image from the^(64)CuCl_(2)+TCPP image.The experimental results considering different^(64)CuCl_(2)doses showed a dosedependent trend.These results demonstrate that a bioluminescence imaging device coupled with different longpass filters and subtraction image processing can confirm the emission spectra and differential fluorescence images of CR-induced TCPP.CONCLUSION This simple method identifies the PS fluorescence emission generated by radionuclide-derived CR and can contribute to accelerating the development of Cherenkov energy transfer imaging and the discovery of new PSs.

基于改进CGAN的海冰SAR-to-Optical影像转换

遥感海冰监测是当前研究热点,通过条件生成对抗网络(CGAN)将海冰SAR影像转换成光学影像,可获得全天时全天候且形象直观的监测数据,但该方法得到的转换结果存在影像模糊、纹理弱化和颜色失真等问题。本文针对以上问题设计了改进的CGAN网络,综合当前的改进方式,新模型在网络结构上加入了空洞空间金字塔模块并设计了加入交叉特征融合模块的跳跃连接,使用结构相似性和L1范数联合损失函数。本文选取东波弗特海地区5景Sentinel-1影像和7景Sentinel-2影像开展实验,实验结果表明,改进CGAN转换的影像具有更好的视觉效果,峰值信噪比(PSNR)提高了3.4 dB,结构相似性(SSIM)提高了0.11,均方根误差(RMSE)降低了13%,并且经过转换后的影像比SAR影像海冰分类结果准确度提高了7.33%。

Bioinspired Polarized Optical Flow Enables Turbid Underwater Target Motion Estimation

Underwater target motion estimation is a challenge for ocean military and scientific research.In this work,we propose a method based on the combination of polarization imaging and optical flow for turbid underwater target detection.Polarization imaging can reduce the influence of backscattered light and obtain high-quality images underwater.The optical flow shows the motion and structural information of the target.We use polarized optical flow to obtain the optical flow field and estimate the target motion.The experimental results of different targets under varying water turbidity levels illustrate that our method is realizable and robust.The precision is verified by comparing the results with the precise displacement data and calculating two error measures.The proposed method based on polarized optical flow can obtain accurate displacement information and a good recognition effect.Moving target segmentation based on the Otsu method further proves the superiority of the polarized optical flow under turbid water.This study is valuable for target detection and motion estimation in scattering environments.

NIR-II fluorescence imaging in liver tumor surgery: A narrative review

In liver tumor surgery,the recognition of tumor margin and radical resection of microcancer focis have always been the crucial points to reduce postoperative recurrence of tumor.However,naked-eye inspection and palpation have limited effectiveness in identifying tumor boundaries,and traditional imaging techniques cannot consistently locate tumors in real time.As an intraoperative real-time navigation imaging method,NIRfluorescence imaging has been extensively studied for its simplicity,reliable safety,and superior sensitivity,and is expected to improve the accuracy of liver tumor surgery.In recent years,the research focus of NIRfluorescence has gradually shifted from the-rst near-infrared window(NIR-I,700–900 nm)to the second near-infrared window(NIR-II,1000–1700 nm).Fluorescence imaging in NIR-II reduces the scattering effect of deep tissue,providing a preferable detection depth and spatial resolution while signi-cantly eliminating liver autofluorescence background to clarify tumor margin.Developingfluorophores combined with tumor antibodies will further improve the precision offluorescence-guided surgical navigation.With the development of a bunch offluorophores with phototherapy ability,NIR-II can integrate tumor detection and treatment to explore a new therapeutic strategy for liver cancer.Here,we review the recent progress of NIR-IIfluorescence technology in liver tumor surgery and discuss its challenges and potential development direction.

Automatic diagnosis of diabetic retinopathy using vision transformer based on wide-field optical coherence tomography angiography

Diabetic retinopathy(DR)is one of the major causes of visual impairment in adults with diabetes.Optical coherence tomography angiography(OCTA)is nowadays widely used as the golden criterion for diagnosing DR.Recently,wide-field OCTA(WF-OCTA)provided more abundant information including that of the peripheral retinal degenerative changes and it can contribute in accurately diagnosing DR.The need for an automatic DR diagnostic system based on WF-OCTA pictures attracts more and more attention due to the large diabetic population and the prevalence of retinopathy cases.In this study,automatic diagnosis of DR using vision transformer was performed using WF-OCTA images(12 mm×12 mm single-scan)centered on the fovea as the dataset.WF-OCTA images were automatically classified into four classes:No DR,mild nonproliferative diabetic retinopathy(NPDR),moderate to severe NPDR,and proliferative diabetic retinopathy(PDR).The proposed method for detecting DR on the test set achieves accuracy of 99.55%,sensitivity of 99.49%,and specificity of 99.57%.The accuracy of the method for DR staging reaches up to 99.20%,which has been proven to be higher than that attained by classical convolutional neural network models.Results show that the automatic diagnosis of DR based on vision transformer and WF-OCTA pictures is more effective for detecting and staging DR.

Multiplexed stimulated emission depletion nanoscopy(mSTED)for 5-color live-cell long-term imaging of organelle interactome

Stimulated emission depletion microscopy(STED)holds great potential in biological science applications,especially in studying nanoscale subcellular structures.However,multi-color STED imaging in live-cell remains challenging due to the limited excitation wavelengths and large amount of laser radiation.Here,we develop a multiplexed live-cell STED method to observe more structures simultaneously with limited photo-bleaching and photo-cytotoxicity.By separating live-cell fluorescent probes with similar spectral properties using phasor analysis,our method enables five-color live-cell STED imaging and reveals long-term interactions between different subcellular structures.The results here provide an avenue for understanding the complex and delicate interactome of subcellular structures in live-cell.

The Application Progress of Skin Imaging Technology in Psoriasis

Skin imaging technologies such as dermoscopy, high-frequency ultrasound, reflective confocal microscopy and optical coherence tomography are developing rapidly in clinical application. Skin imaging technology can improve clinical diagnosis rate, and its non-invasiveness and repeatability make it occupy an irreplaceable position in clinical diagnosis. With the “booming development” of medical technology, skin imaging technology can improve clinical diagnosis rate. Researchers have made significant advances in assisting clinical diagnosis, prediction, and treatment of disease. This article reviews the application and progress of skin imaging in the diagnosis of psoriasis.

A promising approach for quantifying focal stroke modeling and assessing stroke progression:optical resolution photoacoustic microscopy photothrombosis

To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly age-related.In this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed laser.We observed the molding process in mice of different ages and presented age-dependent vascular embolism differentiation.Moreover,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a stroke.Our imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.