Recent advances in optical techniques for dynamically probing cellular mechanobiology

Cellular mechanotransduction characterized by the transformation of mechanical stimuli into biochemical signals,represents a pivotal and complex process underpinning a multitude of cellular functionalities.This process is integral to diverse biological phenomena,including embryonic development,cell migration,tissue regeneration,and disease pathology,particularly in the context of cancer metastasis and cardiovascular diseases.Despite the profound biological and clinical significance of mechanotransduction,our understanding of this complex process remains incomplete.The recent development of advanced optical techniques enables in-situ force measurement and subcellular manipulation from the outer cell membrane to the organelles inside a cell.In this review,we delved into the current state-of-the-art techniques utilized to probe cellular mechanobiology,their principles,applications,and limitations.We mainly examined optical methodologies to quantitatively measure the mechanical properties of cells during intracellular transport,cell adhesion,and migration.We provided an introductory overview of various conventional and optical-based techniques for probing cellular mechanics.These techniques have provided into the dynamics of mechanobiology,their potential to unravel mechanistic intricacies and implications for therapeutic intervention.

Editorial: Advances in Optical Techniques for Mechanical Measurements

Recently, optical techniques have attracted great attention due to their excellent non-destructive, non-contact, high-resolution, and full-field characteristics. Applications can be found in diverse fields such as precision mechanics and manufacturing, aerospace and automotive testing and inspection, materials science, and biomedical engineering. Advances in Optical Techniques for Me- chanical Measurements presents the latest research progresses in several widely used optical techniques with applications in preci- sion mechanical engineering.

Multidither coherent optical adaptive technique for deep tissue two-photon microscopy

Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging.Multidither coberent optical adaptive technique(COAT)can correct the scattered wavefront in parallel.However,the determination of the corrective phases may not be completely accurate using conventional method,which undermines the performance of this technique.In this paper,we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fuorescence signal.A numnerical simulation model is established to study the performance of adaptive optics in two-photon micros-copy by combining scalar diffraction theory with vector diffraction theory.The results show that the distortion of the wavefront can be corrected more thoroughly with our method in two-photon imaging.In our simulation,with the scattering from a 450-mn-thick mouse brain tissue,excitation focal spots with higher peak-to background ratio(PBR)and images with higher contrast can be obtained.Hence,further enhancement of the multidither COAT correction performance in two-photon imaging can be expected.

A new distribution scheme of decryption keys used in optical verification system with multiple-wavelength information

A new distribution scheme of decryption keys used in optical verification systems is proposed. The encryption procedure is digitally implemented with the use of an iteration algorithm in computer. Three target images corresponding to three wavelengths are encoded into three sets of phase-only masks （POMs） by a special distributing method. These three sets of POMs are assigned to three authorized users as the personal identification. A lensless optical system is used as the verification system. In the verification procedure, every two of the three authorized users can pass the verification procedure cooperatively, but only one user cannot do. Numerical simulation shows that the proposed distribution scheme of decryption keys not only can improve the security level of verification system, but also can bring convenience and flexibility for authorized users.

Tunable wavelength filters using polymer long-period waveguide gratings based on metal-cladding directly defined technique

In this work, long-period waveguide grating-based tunable wavelength filters using organic–inorganic grafting poly（methyl methacrylate）（PMMA） materials are designed and fabricated by metal-cladding directly defined technique.The thermal stabilities and optical properties of the organic–inorganic grafting PMMA core materials are analyzed. Structures and performance parameters of the waveguide gratings and self-electrode heaters are designed and simulated. The contrast of the filter is about 15 d B and the resonant wavelength can be tuned by different electric powers applied to the metal-cladding self-electrode heaters. The temperature sensitivity is 3.5 nm/℃ and the switching time is about 1 ms. The technique is very suitable for realizing the optoelectronic integrated wavelength-division-multiplexing systems.

Trends in in ovo sexing technologies:insights and interpretation from papers and patents

Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception.This review gives a complete overview of the technological approaches reported in papers and patents by performing a thorough search using Web of Science and Patstat/Espacenet databases for papers and patents,respectively.Based on a total of 49 papers and 115 patent families reported until May 2023 worldwide,11 technology categories were defined:6 non-optical and 5 optical techniques.Every category was described for its characteristics while assessing its potential for application.Next,the dynamics of the publications of in ovo sexing techniques in both paper and patent fields were described through growth curves,and the interest or actual status was visualized using the number of paper citations and the actual legal status of the patents.When comparing the reported technologies in papers to those in patents,scientific gaps were observed,as some of the patented technologies were not reported in the scientific literature,e.g.,ion mobility and mass spectrometry approaches.Generally,more diverse approaches in all categories were found in patents,although they do require more scientific evidence through papers or industrial adoption to prove their robustness.Moreover,although there is a recent trend for non-invasive techniques,invasive methods like analyzing DNA through PCR or hormones through immunosensing are still being reported(and might continue to be)in papers and patents.It was also observed that none of the technologies complies with all the industry requirements,although 5 companies already entered the market.On the one hand,more research and harmony between consumers,industry,and governments is necessary.On the other hand,close monitoring of the market performance of the currently available techniques will offer valuable insights into the potential and expectations of in ovo sexing techniques in the poultry industry.

Generation of 207-femtosecond pulses from a ytterbium figure-of-eight fibre laser

We report the generation of 207-fs pulses with 1.2 mW average power at 1036 nm directly from a passively modelocked Yb-doped fibre laser with a nonlinear optical loop mirror for mode-locking and pairs of diffraction gratings for intracavity dispersion compensation. These results imply a 4-fold reduction in pulse duration over previously reported figure-of-eight cavity passively mode-locked Yb-doped fibre lasers. Stable pulse trains are produced at the fundamental repetition rate of the resonator, 24.0 MHz. On the other hand, this laser offers a cleaner spectrum and greater stability and is completely self-starting.

Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples

Stimulated emission depletion(STED) microscopy is one of far-field optical microscopy techniques that can provide sub-diffraction spatial resolution. The spatial resolution of the STED microscopy is determined by the specially engineered beam profile of the depletion beam and its power. However, the beam profile of the depletion beam may be distorted due to aberrations of optical systems and inhomogeneity of a specimen's optical properties, resulting in a compromised spatial resolution. The situation gets deteriorated when thick samples are imaged. In the worst case, the severe distortion of the depletion beam profile may cause complete loss of the superresolution effect no matter how much depletion power is applied to specimens. Previously several adaptive optics approaches have been explored to compensate aberrations of systems and specimens. However, it is difficult to correct the complicated high-order optical aberrations of specimens. In this report, we demonstrate that the complicated distorted wavefront from a thick phantom sample can be measured by using the coherent optical adaptive technique. The full correction can effectively maintain and improve spatial resolution in imaging thick samples.

Preliminary study of a dispersed fringe type sensing system

Telescopes with large aspherical primary mirrors collect more light and are therefore sought after by astronomers. Instead of using a single large one-piece mirror, smaller segments can be assembled into a useable telescopic primary. Because the segments must fit together to create the effect of a single mirror, segmented optics present unique challenges to the fabrication and testing that are absent in monolithic optics. A dispersed fringe sensor （DFS） using a broadband point source is an efficient method for cophasing and is also highly automated and robust. Unlike the widely adopted Shack- Hartmann Wavefront sensor and curvature wavefront sensor with edge sensors for calibration of relative pistons, DFS can estimate the piston between segments by only using the spectrum formed by the transmissive grating＇s dispersion, and therefore can replace the edge sensors, which are difficult to calibrate. We introduce the theory of the DFS and Dispersed Hartmann Sensor （DHS） for further utilization of the coarse phasing method of DFS. According to the theory, we bring out the preliminary system design of the cophasing experimental system based on DFS and DHS which is now established in our institute. Finally, a summary is reached.

A new demodulation technique for optical fiber interferometric sensors with[3×3]directional couplers

Optical fiber interferometric sensors based on [3×3] couplers have been used in many fields. A new technique is proposed to demodulate output signals of this kind of sensors. The technique recovers the signal of interest by fitting coefficients of elliptic （Lissajous） curves between each fiber pair. Different from other approaches, this technique eliminates the dependence on the idealization of [3×3] coupler, provides enhanced tolerance to the variance of photoelectric converters, and is anti-polarization in a certain extent. The main algorithm has been successfully demonstrated both by numerical simulation and experimental result.

Arbitrary and reconfigurable optical vortex generation:a high-efficiency technique using director-varying liquid crystal fork gratings

A high-efficiency technique for optical vortex(OV) generation is proposed and demonstrated. The technique is based on liquid crystal fork gratings with space-variant azimuthal orientations, which are locally controlled via polarization-sensitive alignment layers. Thanks to the optical rewritability of the alignment agent and the dynamic image generation of the digital micro-mirror device, fork gratings can be instantly and arbitrarily reconfigured.Corresponding optical vortices carrying arbitrary azimuthal and radial indices are demonstrated with a conversion efficiency of 98.5%, exhibiting features of polarization control and electrical switching. The technique may pave a bright road toward OV generation, manipulation, and detection.

Active Optics in LAMOST

Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST) is one of the major national projects under construction in China. Active optics is one of the most important technologies for new large telescopes. It is used for correcting telescope errors generated by gravitational and thermal changes. Here, however, we use this technology to realize the configuration of LAMOST,—a task that cannot be done in the traditional way. A comprehensive and intensive research on the active optics used in LAMOST is also reported, including an open-loop control method and an auxiliary closed-loop control method. Another important development is in our pre-calibration method of open-loop control, which is with some new features: simultaneous calculation of the forces and displacements of force actuators and displacement actuators; the profile of mirror can be arbitrary; the mirror surface shape is not expressed by a fitting polynomial, but is derived from the mirror surface shape formula which is highly accurate; a proof is given that the solution of the pre-calibration method is the same as the least squares solution.

New vacuum solar telescope and observations with high resolution

The New Vacuum Solar Telescope （NVST） is a one meter vacuum solar telescope that aims to observe fine structures on the Sun. The main goals of NVST are high resolution imaging and spectral observations, including measurements of the solar magnetic field. NVST is the primary ground-based facility used by the Chinese solar research community in this solar cycle. It is located by Fuxian Lake in southwest China, where the seeing is good enough to perform high resolution observations. We first introduce the general conditions at the Fuxian Solar Observatory and the primary science cases of NVST. Then, the basic structures of this telescope and instruments are described in detail. Finally, some typical high resolution data of the solar photosphere and chromosphere are also shown.

A new burst assembly technique for supporting QoS in optical burst switching networks

This letter proposes a new burst assembly technique for supporting QoS in optical burst switching (OBS) networks. It consists of the adaptive-threshold burst assembly mechanism and QoS-based random offset-time scheme. The assembly mechanism, which is fit well to multi-class burst assembly, not only matches with IP QoS mechanism based on packet classification, and also utilizes fairly and efficiently assembly capacity. Based on token-bucket model and burst segment selective discard (BSSD), the offset-time scheme can smooth the traffic to support OBS QoS. The simulation results show that the technique can improve the performance in terms of packet loss probability (PLP).

Stochastic parallel gradient descent based adaptive optics used for a high contrast imaging coronagraph

An adaptive optics （AO） system based on a stochastic parallel gradient descent （SPGD） algorithm is proposed to reduce the speckle noises in the optical system of a stellar coronagraph in order to further improve the contrast. The principle of the SPGD algorithm is described briefly and a metric suitable for point source imaging optimization is given. The feasibility and good performance of the SPGD algorithm is demonstrated by an experimental system featured with a 140-actuator deformable mirror and a Hartrnann-Shark wavefront sensor. Then the SPGD based AO is applied to a liquid crystal array （LCA） based coronagraph to improve the contrast. The LCA can modulate the incoming light to generate a pupil apodization mask of any pattern. A circular stepped pattern is used in our preliminary experiment and the image contrast shows improvement from 10^-3 to 10^-4.5 at an angular distance of 2A/D after being corrected by SPGD based AO.

The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST)

The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST, also called the Guo Shou Jing Telescope) is a special reflecting Schmidt telescope. LAMOST’s special design allows both a large aperture (effective aperture of 3.6 m–4.9 m) and a wide field of view (FOV) (5°). It has an innovative active reflecting Schmidt configuration which continuously changes the mirror’s surface that adjusts during the observation process and combines thin deformable mirror active optics with segmented active optics. Its primary mirror (6.67m×6.05 m) and active Schmidt mirror (5.74m×4.40 m) are both segmented, and composed of 37 and 24 hexagonal sub-mirrors respectively. By using a parallel controllable fiber positioning technique, the focal surface of 1.75 m in diameter can accommodate 4000 optical fibers. Also, LAMOST has 16 spectrographs with 32 CCD cameras. LAMOST will be the telescope with the highest rate of spectral acquisition. As a national large scientific project, the LAMOST project was formally proposed in 1996, and approved by the Chinese government in 1997. The construction started in 2001, was completed in 2008 and passed the official acceptance in June 2009. The LAMOST pilot survey was started in October 2011 and the spectroscopic survey will launch in September 2012. Up to now, LAMOST has released more than 480 000 spectra of objects. LAMOST will make an important contribution to the study of the large-scale structure of the Universe, structure and evolution of the Galaxy, and cross-identification of multiwaveband properties in celestial objects.

Pulse reference-based compensation technique for intensity-modulated optical fiber sensors

We propose a compensation technique based on pulse reference for intensity-modulated optical fiber sensors that can compensate the power fluctuation of the light source, the change of optical components transmission loss, and the coupler splitting ratio. The theoretical principle of this compensation technique is analyzed and a temperature sensor based on fiber coating-covered optical microfiber is carried out to demonstrate the compensation effect. The system noise is measured to be 0.0005 dB with the temperature sensitivity reaching -0.063 dB/℃, and the output drift is 0.006 dB in 2 h at room temperature. The output shows a slight variation （0.0061 dB） when the light source and the common liKht path suffer a 3 dB attenuation fluctuation.

MEMS Test System Based on Virtual Instrument Technology

On account of the multiformity of MEMS devices, it is necessary to integrate with some optical measurement techniques for meeting static and dynamic unit test requirements. In this paper, an automated MEMS test system is built of some commercially available components and instruments based on virtual instrument technology. The system is integrated with stroboscopic imaging, computer micro-vision, microscopic Mirau phase shifting interferometry, and laser Doppler vibrometer, and is used for the measurement of full-view in-plane and out-of-plane geometric parameters and periodical motions and single spot out-of-plane transient motion. The system configuration and measurement methods are analyzed, and some applications of the measurement of in-plane and out-of-plane dimensions and motions were described. The measurement accuracy of in-plane dimensions and out-of-plane dimensional is better than 0.2 um and 5 nm respectively. The resolution of measuring in-plane and out-of-plane motions is better than 15 nm and 2 nm respectively.

Frequency-domain photoacoustic technique formeasuring absolute optical absorption coefficient using three-chamber cell

Frequency-domain photoacoustic (PA) technique for immediate measuring absolute optical absorption coefficient is presented. It is based on determination of the ratio of the two spectrum amplitudes of the differential PA signal. The sample is placed into the lateral chamber of a three chamber PA cell. The difference of pressure variations (differential PA signal) excited in two other chambers as a result of laser pulse absorption by the sample is recorded. Presented PA technique was tested experimentally by measuring water absorption coefficient at an IR wavelength.