Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence. The optimal medium, composition （g·L^-1）, containing sucrose 66.0, yeast powder 10.0, silkworm chrysalises digest 30.0, MgSO4· 7H2O 0.4, and KH2PO4 0.4, Was found using fractional factorial design ancl a central composite design, and the optimization of cultural conditions obtained a result of seed age 6 days, inoculum size 6% （by volume）, initial pH 5.6, temperature 24℃, shaking speed 160 ·＇min^-1 by one-factor-at-a-time method. The maximum biomass reached about 20.2 g·L^-1 after 90 hours culture under the optimal conditions. Elementary nharmaeclogical actlwtties showed that mycelia of C. ophioglossoides L2 from submerged culture promoted Uterus growth in estrogen- depleted mice. In the 15-litre scale-up fermentation, the mycelial biomass was around 19.1 g·L^-1, indicating a promising prospect for this biotechnoloagy and the potency to develoo its medical value.

Preliminary results on relationship between thermal diffusivity and porosity of sea ice in the Antarctic

The in situ sea-ice temperature, salinity and density observed from Chinese Antarctic Zhongshan Station have been applied to calculate the vertical profile of sea ice porosity. Based on numerical method, a number of schemes on sea - ice thermal diffusivity versus porosity have been accessed and one optimized scheme is identified by an optimal control model with an advanced, distributing parameter system. For simplicity, the internal heating source item was neglected in the heat conduction equation during the identification procedure. In order to illustrate the applicability of this identified scheme, the vertical ice temperature profiles have been simulated and compared with measurements, respectively by using identified scheme and by classical thermodynamic formulae. The comparisons indicated that the scheme describing sea - ice thermal diffusivity and porosity is reasonable. In spite of a minor improvement of accuracy of results against in situ data, the identified scheme has a more physical meaning and could be used potentially in various applications.

Regulation Flexibility Assessment and Optimal Aggregation Strategy of Greenhouse Loads in Modern Agricultural Parks

With advances in modern agricultural parks,the rural energy structure has undergone profound change,leading to the emergence of an agricultural energy internet.This integrated system combines agricultural energy utilization,the information internet,and agricultural production.Accordingly,this study proposes a regulation flexibility assessment approach and optimal aggregation strategy of greenhouse loads(GHLs)for modern agricultural parks.First,taking into account the operational characteristics of typical GHLs,refined load demand models for lighting,humidification,and temperature-controlled loads are established.Secondly,the recursive least squares method-based parameter identification method is designed to accurately determine key GHL model parameters.Finally,based on the regulation flexibility of quantitatively evaluated GHLs,GHLs are optimally aggregated into multiple flexible aggregators considering minimal operational cost and greenhouse environmental constraints.The results indicate that the proposed regulation flexibility assessment approach and optimal aggregation strategy of GHLs can alleviate the peak regulation pressure on power grids by flexibly shifting the load demands of GHLs.

Optimization of Identifying Point Pollution Sources for the Convection-Diffusion-Reaction Equations

In this paper,we consider the optimization problem of identifying the pollution sources of convection-diffusion-reaction equations in a groundwater process.The optimization model is subject to a convection-diffusion-reaction equation with pumping point and pollution point sources.We develop a linked optimization and simulation approach combining with the Differential Evolution(DE)optimization algorithm to identify the pumping and injection rates from the data at the observation points.Numerical experiments are taken with injections of constant rates and timedependent variable rates at source points.The problem with one pumping point and two pollution source points is also studied.Numerical results show that the proposed method is efficient.The developed optimized identification approach can be extended to high-dimensional and more complex problems.