Strategic insights into the cultivation of pancreatic cancer organoids from endoscopic ultrasonography-guided biopsy tissue

BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an important factor in maintaining their long-term expansion,which is essential for shortening the time of drug sensitivity analysis and is the basis of PC research.

Analysis of simultaneous effect of water access requirements in Regional Water Corporation ofWest- Azerbaijan and risk factor on cultivation optimal pattern

In vast farms management,especially in multi-crop cultivation case,planning for a combination of crops cultivation has always been of high importance to achieve the maximum income,by taking into account imposed limitations and the risk of different activities.According to the fact that in traditional models of farm programming because of not attention to risk,its results differ from what farmer do in reality,considering the risk farm programming should be well thought.MOTAD model(Minimization of the Total Absolute Deviation)is a mathematic programming model that enters risk into decision making related to farm activities and provides different programs.A main feature of this model is that the risk is calculated by negative deviation from efficiency amount as total multiplication.In recent years,serious declining of water table problem forced Regional Water Corporation of West-Azerbaijan province to impose limitations for extra water use of wells in order to sustain"Mako,Poldasht"water resources.These limitations result in lowering available water level(The most important input for farming),and create changes in cropping patterns and income level of farmers in this area.The aim of this study is analyzing improved cropping considering water limitations and in risk conditions.Data have been collected from farmers and Agriculture"Jihad"Management of town of Mako for duration of 1387-1388.According to the results of the research,the real cultivation plan that is now being implemented throughout the region carries a high risk and low income.Based on results,if the farmer uses the optimal cultivation pattern during the so-called water access limitation,watermelon harvest will be excluded from the optimal cultivation pattern.Also,alfalfa will enter the cultivation plan with the expected income level of 1067 billion Rials(100 Rials=US$0.01).If the farmer uses improved cropping pattern in limitations in risk it will decrease total income as 171.36 billion Rials,to when the water limitations is not implemented.