Effects of Nitrogen Fertigation Rate on Protein Components in Grains and Processing Quality of Different Wheat Varieties

[Objective] This study aimed to explore the effects of nitrogen fertigation rate on the protein components in grains and processing quality of different wheat varieties. [Method] Under the condition of higher soil fertility, different amounts of N-fertilizer were applied in the plots, and then the contents of total protein and its components, percentage of the component content to total protein content as well as the processing quality of grains of two strong-gluten wheat varieties （Linyou145 and Zhengmai9023） and two weak-gluten wheat varieties （Ningmai9 and Baofeng949） were determined. [Result] The contents of total protein and globulin, gliadin and glutenin were improved significantly with the increase of the N-fertilizer amount; but the content of albumin did not show remarkable increase; in addition, the percentage of each protein component was relative stable and did not increase significantly. Increase in the amount of N-fertilizer improved the sedimentation value, wet gluten content, loaf volume and loaf score, decreased the volume weight of grain. [Conclusion] This study provideed theoretical support for high-quality wheat production.

Influence of Organic and Inorganic Nitrogen on Grain Yield andYield Components of Hybrid Rice in Northwestern Pakistan

Field experiments were conducted to assess the impact of various organic sources, inorganicnitrogen （N） and the different combinations of inorganic N （urea） ＋ organic source on the yieldcomponents （YC） and grain yield （GY） of hybrid rice （Oryza sativa L., Pukhraj） under rice-wheat system.The experiments were conducted at Batkhela （Malakand）, Northwestern Pakistan, in 2011 and 2012.Our results revealed that YC and GY ranked first for the hybrid rice when applied with sole inorganic N（urea）, followed by the application of N in mixture （urea ＋ organic sources）, while the control plots （no Napplied） ranked in the bottom. Among the six organic sources （three animal manures： poultry, sheepand cattle; three crop residues： onion, berseem and wheat）, application of N in the form of poultrymanure was superior in terms of higher YC and GY. When applying 120 kg/hm2 N source, 75% N fromurea ＋ 25% N from organic source resulted in higher YC and GY in 2011, while applying 50% N fromurea ＋ 50% N from organic sources caused higher YC and GY in 2012. Therefore, the combinedapplication of N sources in the form of urea ＋ organic source can produce good performances in termsof higher YC and GY of rice under rice-wheat cropping system.

Regulation effects of water and nitrogen on yield,water,and nitrogen use efficiency of wolfberry

Wolfberry(Lycium barbarum L.)is important for health care and ecological protection.However,it faces problems of low productivity and resource utilization during planting.Exploring reasonable models for water and nitrogen management is important for solving these problems.Based on field trials in 2021 and 2022,this study analyzed the effects of controlling soil water and nitrogen application levels on wolfberry height,stem diameter,crown width,yield,and water(WUE)and nitrogen use efficiency(NUE).The upper and lower limits of soil water were controlled by the percentage of soil water content to field water capacity(θ_(f)),and four water levels,i.e.,adequate irrigation(W0,75%-85%θ_(f)),mild water deficit(W1,65%-75%θ_(f)),moderate water deficit(W2,55%-65%θ_(f)),and severe water deficit(W3,45%-55%θ_(f))were used,and three nitrogen application levels,i.e.,no nitrogen(N0,0 kg/hm^(2)),low nitrogen(N1,150 kg/hm^(2)),medium nitrogen(N2,300 kg/hm^(2)),and high nitrogen(N3,450 kg/hm^(2))were implied.The results showed that irrigation and nitrogen application significantly affected plant height,stem diameter,and crown width of wolfberry at different growth stages(P<0.01),and their maximum values were observed in W1N2,W0N2,and W1N3 treatments.Dry weight per plant and yield of wolfberry first increased and then decreased with increasing nitrogen application under the same water treatment.Dry weight per hundred grains and dry weight percentage increased with increasing nitrogen application under W0 treatment.However,under other water treatments,the values first increased and then decreased with increasing nitrogen application.Yield and its component of wolfberry first increased and then decreased as water deficit increased under the same nitrogen treatment.Irrigation water use efficiency(IWUE,8.46 kg/(hm^(2)·mm)),WUE(6.83 kg/(hm^(2)·mm)),partial factor productivity of nitrogen(PFPN,2.56 kg/kg),and NUE(14.29 kg/kg)reached their highest values in W2N2,W1N2,W1N2,and W1N1 treatments.Results of principal component analysis(PCA)showed that yield,WUE,and NUE were better in W1N2 treatment,making it a suitable water and nitrogen management mode for the irrigation area of the Yellow River in the Gansu Province,China and similar planting areas.

Effects of Nitrogen Fertilizer on Seed Yields and Yield Components of Zoysia japonica Established by Seeding and Transplant

The study was conducted to determine the optimum fertilizer-N application rate formaximum seed yields of Zoysiagrass stands from seeding and transplant respectively, atJiaozhou, Shandong Province, China from 2001 to 2003. In the third year after establishment,seed yields and yield components for both stands showed a similar response to fertilizer-N. Maximum fertile tiller numbers (3342 heads m-2 and 2941 heads m-2 from stands seeded inrows and transplanted, respectively) and the highest seed yields (844.50kgha-1 and 874.65kg ha-1 from stands seeded in rows and transplanted, respectively) were obtained at a Nfertilizer rate of 20kgha-1 in autumn and 10kgha-1 in spring (30kgha-1 N in total). Thefertile tillers and seed yields decreased with further increasing of N fertilizer rate.Fertilizer-N application could increase the length of spike, spikelets per fertiletiller, seed number per spike, setting percentage and thousand seed weight. The 1000-seed weight and the length of spike from transplant plots were higher than that fromseeding plots. The optimal harvest time of zoysiagrass at Jiaozhou was on the 36th dayafter peak anthesis, near June 15th, when the seed moisture content was 26-28%.

The Changing Characteristics of Profile Distribution of Organic Nitrogen Components in Apple-pear Orchard Soil

In order to evaluate the effects of soil depth on the contents of soil organic nitrogen,organic nitrogen forms in apple-pear orchard soil profile were quantified using the method proposed by Bremner in 1965.The results indicated that in addition to the amino sugar-N,all the soil organic N components within the same soil layer in wasteland were more than those in apple-pear orchard soil;with the layer depth increasing,the contents of different organic nitrogen forms in apple-pear orchard soil and wasteland were decreased;and the proportion of each organic N component within total hydrolysable N was different,and the percentages of ammonia N and amino acid-N components within total hydrolysable N were higher,especially the percentage of ammonia N components within total hydrolysable N was the highest.

The Effects of Different Levels of Nitrogen on Yield and Yield Components of Rainfed Wheat in Two Regions of North Khorasan

To study the effects of different levels of nitrogen fertilizer on yield and yield components of cultivars of rainfed wheat a study was done in the crop year 2012-2013 as factorial in a randomized complete block design with 3 replications, in 2 research stations of Kohne Kand city in Bojnord and Shirvan dryland research station. The treatments of study contain cultivars of wheat in 4 levels (Rasad, Sabalan, Cross Sabalan and Azar 2) and 4 levels of nitrogen fertilizer (75, 50, 25 and 0 kilograms nitrogen per hectare). The results showed that the yield in Shirvan was significantly higher than that in Bojnoord. Moreover, the impact of cultivar and nitrogen on yield and yield components was significant in both regions and among different cultivars Azar 2 has the highest yield in both regions and the lowest yield was for cross Sabalsn in Shirvan and Sabalan in Bojnoord. Furthermore, yield increased by increasing the nitrogen in both regions but there was no significant difference between levels 50 and 75 kilograms per hectare. Among yield components except the number of spikes per square meter, others had a high correlation with yield.

Response of Yield, Yield Components and Fiber Properties of Cotton to Different Application Rates of Nitrogen and Boron

Nitrogen （N） was applied at rates of 0, 100, 200 and 300 kg.ha^-1 and boron （B） was applied as foliar at rates 0, 500 and 1000 g.ha^-1 to study the effect of different application rates of nitrogen and boron fertilizers on yield, yield components and fiber properties of cotton. Statistical results of study showed that N application significantly （P 〈 0.05） enhanced boll number, boll weight, seed cotton weight of boll, seed cotton yield and lint yield. Results of study also indicated that the maximum seed cotton yield was recorded in case of 200 kg.ha^-1 N application rate, and this application rate resulted in 19.6% increased seed cotton yield. Statistical results also indicated that foliar application of B significantly enhanced boll number, boll weight, seed cotton yield and lint yield. Results also demonstrated that the maximum seed cotton yield was obtained in case of 1000 g.hal foliar application of B, and this foliar application rate resulted in 25% increased seed cotton yield. Statistical results showed that effect of different application rates of N was not significant for all fiber properties （fiber length, fiber strength and fiber fineness）. Conversely, results of study indicated that different application rates of B significantly affected some fiber properties.

氮、磷、钾配施对葛根生长及品质的影响

为探明氮、磷、钾肥配施对葛根生长及品质的影响,为葛根高效栽培提供合理的施肥技术支撑和理论依据,采用L_(9)(3~4)正交试验设计,研究氮、磷、钾配施对葛根产量和主要功能成分的影响,明确研究区葛根氮、磷、钾的最佳施用量。结果表明,不同施肥方案对葛根产量和功能成分的影响显著,其中以N_(3)P_(1)K_(2)(N 270kg/hm^(2),P_(2)O_(5)45 kg/hm^(2),K_(2)O 90 kg/hm^(2))处理的产量和总黄酮含量最高,分别为2.13 kg/株和83.10 mg/g;N_(1)P_(3)K_(2)(N 90 kg/hm^(2),P_(2)O_(5)135 kg/hm^(2),K_(2)O 90 kg/hm^(2))的葛根素含量最高,为24.85 mg/g,N_(1)P_(2)K_(3)(N 90 kg/hm^(2),P_(2)O_(5)90 kg/hm^(2),K_(2)O 135 kg/hm^(2))处理的大豆苷和大豆苷元含量均为最高,分别为22.96和2.55 mg/g。氮、磷、钾肥配施对葛根产量和葛根素含量影响的大小顺序为氮肥>钾肥>磷肥。以葛根产量为目标的最佳施肥量为N 270 kg/hm^(2)、P_(2)O_(5)135 kg/hm^(2)、K_(2)O 90 kg/hm^(2),以葛根品质提升为目标的最佳施肥量为N 180 kg/hm^(2)、P_(2)O_(5)135 kg/hm^(2)、K_(2)O 90 kg/hm^(2)。

4种氮肥用量对‘紫嫣’茶树生长发育的影响

【目的】研究氮肥用量对‘紫嫣’茶树生长和生化成分含量的影响,为‘紫嫣’茶树高产高效栽培体系的建立提供理论依据。【方法】试验设置4个尿素水平(0、375、525、675 kg/hm^(2)),比较不同氮肥用量下‘紫嫣’茶树产量、品质成分和氮磷钾含量的差异。【结果】不同施氮量处理均能不同程度地促进‘紫嫣’茶树新梢生长、芽叶品质成分含量及氮磷钾的积累,提高土壤酶活性,以560 kg/hm^(2)尿素处理效果显著。此处理下,‘紫嫣’茶树春茶产量显著增加27.44%,春梢的水浸出物、氨基酸和茶多酚含量显著提高10.41%、27.38%、10.09%,叶绿素、类胡萝卜素和花青素显著提高37.35%、25.00%、19.57%。而夏梢的水浸出物、茶多酚含量和叶绿素总量较不追肥处理仍显著提高。【结论】适量氮肥利于‘紫嫣’茶树生长,使产量和品质提高,但随着氮肥用量增加,氮肥的促进效应降低。因此,对‘紫嫣’茶园(黄壤土pH=4.66,有机质27.43 g/kg,全氮1.24 g/kg,速效磷32.05 mg/kg,速效钾81 mg/kg)宜追施525 kg/hm^(2)尿素。

手性分子构象分析装置圆二色光谱仪的加强与改造

圆二色光谱仪是对手性分子进行构象分析和理化性质表征的重要仪器.然而长期高强度的使用会产生光谱仪光学元件劣化、检测器灵敏度降低等问题.通过优化分光系统的光学元件提升光传输效率,升级具有更高量子效率的大面积雪崩硅光电二极管检测器,进而大幅提高仪器信噪比,同时将测量波长范围从175~900 nm扩展到175~1150 nm.在氙灯被点燃之前用氮气彻底净化仪器,把氧气排除在光谱仪之外是非常重要的操作步骤.安装具有监视、计划、流量设置、报警和辅助控制等功能的活性氮监测系统,可以实时监测控制仪器内部氮气吹扫情况.通过升级改造,既加强了仪器的性能,又节省了氮气消耗成本,同时仪器使用寿命将进一步延长.

水肥一体化下施氮量对土壤有机氮组分及玉米产量的影响

为进一步优化玉米水肥一体化施肥技术,促进氮肥有效转化利用,研究不同施氮量对土壤有机氮组分及玉米产量的影响。结果表明,随施氮量的增加,土壤酸解总氮、酸解铵态氮、酸解氨基酸态氮和酸解氨基糖态氮含量呈先上升后下降趋势,当施氮量为210 kg·hm^(-2)(N2处理)时,以上4种有机氮组分含量最高。酸解铵态氮是影响玉米产量的关键因素,N2处理下酸解铵态氮含量较N3(252 kg·hm^(-2))、N4(273 kg·hm^(-2))、N1(147 kg·hm^(-2))和N0(0 kg·hm^(-2))分别显著提高3.55%、9.86%、20.37%和170.21%;产量分别显著提高1.58%、2.97%、18.43%和112.89%。综上,N2处理(施氮肥210 kg·hm^(-2))具有更高的供氮潜力,是较为理想的施氮量。

施氮对龙燕1号燕麦种子产量和产量组分的影响

对龙燕1号燕麦(Avena sativa cv.Longyan No.1)进行不同施氮(以下简称施N)处理,探讨不同施N对其种子产量和产量性状的影响。结果表明,龙燕1号燕麦在施N量60 kg/hm^(2)时株高最高、主穗籽粒数最多、主穗穗粒最重、千粒重最重、轮层数最多,在施N量90 kg/hm^(2)下穗长最长、主穗小穗数最多。龙燕1号燕麦在施N量60 kg/hm2处理下种子产量达到最高,为4760.2 kg/hm^(2),其种子产量与施N量间的数量关系符合y=-0.0499x^(2)+8.3261x+4403(R^(2)=0.965)。株高和千粒重对种子产量的增加直接作用效应最大。因此,在哈尔滨地区燕麦种子生产中,施N量为60 kg/hm^(2),种子产量最高且经济有效。

人工修复边坡植被演替初期有机碳组分及土壤生态化学计量特征

[目的]研究人工修复边坡演替初期土壤有机碳组分、碳氮磷含量及其化学计量比,揭示气候因子对人工修复边坡演替初期土壤有机碳组分、碳氮磷含量及其化学计量比的影响,以期为后期人工修复边坡的养护提供指导和建议,确保人工修复边坡的正向演替。[方法]在湛江、梧州、宜昌、南阳、焦作、潍坊、加查采集了初期基材配制相同、植物配制相近,修复年限为2~4年的植被混凝土人工修复边坡土样,通过实验获得土样有机碳组分、碳、氮、磷的数据,采用冗余分析,开展气候因子对人工修复边坡演替初期土壤有机碳组分、碳氮磷含量及其化学计量比的影响研究。[结果]人工修复边坡演替初期F_(1)含量的变化范围为1.10~3.64 g/kg;F_(2)含量的变化范围为1.33~3.04 g/kg;F_(3)含量的变化范围为2.02~3.34 g/kg;F_(4)含量的变化范围为10.86~15.24 g/kg;TOC含量变化范围为15.52~24.71 g/kg;TN含量变化范围为0.61~0.93 g/kg;TP含量的变化范围为0.81~1.22 g/kg;C∶N变化范围为23.55~27.95;C∶P变化范围为17.26~23.09;N∶P的变化范围为0.72~0.98;C∶N∶P的变化范围为17.26∶0.75∶1~24.81∶0.95∶1。[结论]演替初期人工修复边坡碳磷表现为中等及以上,氮表现为缺乏;有机碳组分以惰性有机碳为主且有机碳稳定性较强。有机碳组分的主要影响因子为年平均温度、物种丰富度和年平均降水;土壤碳氮磷含量及其化学计量比主要影响因子为年平均温度、物种丰富度、pH和容重。

溶解有机氮组分对莱州湾硅藻-甲藻演变的影响及其动力学研究

本文针对莱州湾硅藻向甲藻优势种群演替的潜在问题,设置岸基培养实验,研究溶解有机氮(DON)及其亲水和疏水组分在不同氮磷比条件下对浮游植物群落结构的影响。结果表明,初始无机态氮磷营养盐浓度不受限实验条件下,硅藻(菱形藻(Nitzchia spp.)、扁面角毛藻(Chaetoceros compressus)等)因更强的溶解无机氮(DIN)亲和力(吸收速率常数/半饱和常数)而成为优势藻;高氮磷比和疏水性DON实验条件下,甲藻(塔玛亚历山大藻(Alexandrium tamarense))因对DON具有更强的亲和力而在培养后期演变为优势藻。三维荧光光谱和酶活性分析表明,高腐质化指数(HIX=1.17)的DON疏水性组分含有较高占比的类腐殖质C峰(27.0%)和M峰(16.1%),以及高氮磷比条件下DON微生物降解速率慢等原因,是促进甲藻以DON类腐殖质组分为氮源生长的内在原因;而高生物指数(BIX=1.29)的DON亲水组分含有高占比的类蛋白质吸收峰T1峰(42.5%)和B峰(33.6%),以及低氮磷比条件下DON微生物降解速率快等原因,是促进硅藻以DON类蛋白质组分为氮源生长的内在原因,这也反映在硅藻高活性表达的类氨酸氨肽酶上。

新疆冬小麦耐低氮品种筛选与评价

【目的】探索建立新疆冬小麦氮素品种筛选评价指标,在生产上筛选推荐耐低氮品种,为新疆冬小麦耐低氮品种选育提供方法和材料依据。【方法】于2018~2019年度分别对新疆成功培育的21份冬小麦品种应用“饿氮法”进行耐低氮种质筛选,并应用隶属函数、主成分分析以及聚类分析等方法,对参试冬小麦品种耐低氮程度进行了评估。【结果】两年度连续测试结果表明,在不施氮肥和正常施氮的对比下,株高、蛋白含量均得到不同程度影响,其中蛋白含量敏感度为最高;主成分分析提出3个主要成分,平均贡献率依次为39.63%、19.22%、18.21%,累积贡献率达到了77.06%。【结论】低氮胁迫对冬小麦的产量与相关性状有较大影响,并根据冬小麦种质资源间耐氮程度的不同,结合以上分析方法,能准确评估新疆冬小麦种质资源耐低氮情况。

一种鸡粪来源溶解有机氮在天然海水中不同降解阶段生物可利用性及其组分结构特征

陆源溶解有机氮(DON)是近海环境中的重要氮来源,对海洋生态系统的影响与自身生物可利用性、组分和结构有关。如何表征DON在天然海水老化过程中不断变化的生物可利用性及其结构特征是难点。以畜禽养殖鸡粪作为DON来源,通过1年的天然海水自然降解实验,测定和表征了不同降解阶段DON的理化参数和光谱特征。结果表明,按培养时间划分的生物可利用组分(BDON)中,易利用组分(LBDON,0~12 d)、半易利用组分(HLBDON,12~80 d)和难利用组分(RBDON,80~365 d)占比分别为51.41%、31.79%和2.63%。其中,LBDON和HLBDON主要是三维荧光色氨酸T组分和土壤富里酸D组分,结构上主要是胺基官能团;RBDON主要是可见光富里酸C1组分,主要包括胺基、羟基、芳基和羧基/酯基等官能团;而惰性组分(RDON)主要是三维荧光紫外区富里酸A组分和类腐殖酸E组分,结构上主要包括炔基、芳基和羧基/酯基等。RDON较BDON在芳香性、疏水性和腐殖化指数上均显著升高,分别升高了89.8%、86.5%和39.2%。研究结果有助于深入理解海洋中陆源DON的生物可利用性及其生态效应。

绿洲灌区增密对水氮减量玉米产量的补偿机制

针对河西绿洲灌区水资源短缺、玉米田氮肥施用量高等生产生态问题,在节水减氮条件下,分析增加种植密度补偿水氮减量导致玉米减产的效应,为水氮节约型玉米高效生产提供理论依据与技术支撑。基于2016年布设的裂裂区田间试验,主区为2种灌水定额:灌水减量20%(W1,3240 m^(3) hm^(–2))和传统灌水(W2,4050 m^(3) hm^(–2)),裂区为2种施氮量:减量施氮25%(N1,270 kg hm^(–2))和传统施氮(N2,360 kg hm^(–2)),裂裂区为3种玉米密度:传统种植密度(D1,7.50万株hm^(–2))、增密30%(D2,9.75万株hm^(–2))和增密60%(D3,12.00万株hm^(–2)),通过测定2020—2021年玉米籽粒产量和生物产量,分析干物质积累及其分配、转运特征,量化产量构成因素,明确增密对水氮减量玉米产量的补偿效应及机制。研究表明,减水、减氮降低了玉米籽粒产量和生物产量,而增密30%能够补偿因水氮同步减量造成的产量负效应,且维持较高的施氮量有利于玉米增产节水。W1N1D1(减量灌水减量施氮及传统密度)较W2N2D1(对照:传统灌水传统施氮及传统密度)籽粒产量和生物产量分别降低9.1%~15.0%与10.0%~11.0%,但W1N1D2(减量灌水减量施氮及增密30%)与W2N2D1差异不显著。W1N2D2(减量灌水传统施氮及增密30%)较W2N2D1籽粒和生物产量分别提高12.9%~15.4%与6.4%~12.0%。增密30%能够补偿水氮同步减量造成玉米减产的主要原因是W1N1D2能增加玉米穗数,进而提高玉米灌浆初期至成熟期干物质积累量和苗期到大喇叭口期群体生长速率及花前转运率。增密30%在灌水减量和传统施氮条件下促进玉米增产的主要原因是W1N2D2可增加玉米穗数,提高玉米生育期干物质积累量与群体生长速率,促进穗部干物质分配,提高花前转运量、转运率及转运贡献率。因此,增密30%是绿洲灌区水氮同步减量玉米稳产高产的可行措施,是氮肥不减但减水20%玉米节水增产有效举措。

赖草草地叶片养分、碳组分和防御性化合物对氮添加的响应

本研究依托山西右玉黄土高原赖草(Leymus secalinus)草地2017年建立的氮(Nitrogen, N)添加梯度试验平台(0~32 g·m^(-2)·a^(-1)),探究N添加对优势植物赖草叶片养分[N、磷(Phosphorus, P)、钾(Potassium, K)]、非结构性碳水化合物、结构性碳水化合物和防御性化合物含量的影响。结果表明:N素输入显著提高叶片N,K含量和N∶K和N∶P比值,降低叶片P含量和P∶K比值;N素输入显著降低叶片非结构性碳水化合物和结构性碳水化合物含量;N素输入显著增加叶片总酚、单宁和黄酮类化合物含量;PCA分析表明低N(≤8 g·m^(-2)·a^(-1))和高N(>8 g·m^(-2)·a^(-1))输入下赖草叶片性状存在显著差异,土壤无机N含量、土壤有效N∶P和N∶K是其主要影响因子。以上结果表明N素输入改变了盐渍化草地赖草叶片养分-碳组分-防御性化合物的分配策略。

凯氏定氮法与杜马斯燃烧法检测三氯乙酸沉淀大豆和小麦蛋白中蛋白氮含量研究

目的检验尿素干扰下三氯乙酸沉淀分离大豆和小麦蛋白质的效果。方法本研究以牛奶、大豆蛋白、小麦水溶性组分为参试样品,分别在去离子水、尿素溶液2种溶液体系下分散样品,参考国家标准采用三氯乙酸沉淀蛋白氮,2种蛋白检测方法(凯氏定氮法、杜马斯燃烧法)检测氮含量,并计算氮检测回收率评估三氯乙酸对大豆蛋白、小麦水溶性组分等植物性蛋白的适用性。结果去离子水(无外源氮)分散的上清液经三氯乙酸沉淀,凯氏定氮法和杜马斯燃烧法检测牛奶的回收率分别为114%、101%,大豆的回收率分别为96%、98%,小麦的回收率分别为104%、105%。尿素溶液(有外源氮)分散的上清液经三氯乙酸沉淀,牛奶的回收率分别为99%、94%,大豆的回收率分别为95%、92%,小麦的回收率分别为101%、92%。结论有无尿素干扰时,三氯乙酸均可以沉淀大豆蛋白、小麦水溶性组分中蛋白氮,并可以采用凯氏定氮法、杜马斯燃烧法检测,回收率较好。

基于近红外光谱技术的水稻节间主要碳氮代谢组分分析

近年来,近红外光谱技术在作物样品组分检测和育种材料快速筛选等方面得到广泛应用,而关于水稻源库器官,尤其是水稻节间主要碳氮代谢组分高通量检测的研究鲜见报道。本研究以试验获得的576份代表性节间样本为材料,在获得样本化学实测值的基础上,采集样本的近红外光谱信息,并利用偏最小二乘(partial least square,PLS)法构建水稻节间主要碳氮代谢组分蔗糖、淀粉、总氮模型。模型验证结果显示:水稻节间蔗糖、淀粉、总氮含量PLS模型验证集决定系数分别为0.988、0.977和0.957,相对分析误差均大于3,模型的性能指数分别为85.20、85.80和86.50,表明所建模型预测结果准确可靠。此外,水稻叶片和叶鞘的淀粉、总氮含量和碳氮比的PLS模型也具有较高的精度。本研究构建了水稻节间主要碳氮代谢组分的近红外光谱模型,为水稻栽培生理和高产栽培理论研究提供了技术支撑。