Primary squamous cell carcinoma(SCC) of the liver is rare and reported sporadically.Up to date,only 24 such cases have been reported in the literature.It is associated with hepatic teratoma,congenital cysts,solitary b...Primary squamous cell carcinoma(SCC) of the liver is rare and reported sporadically.Up to date,only 24 such cases have been reported in the literature.It is associated with hepatic teratoma,congenital cysts,solitary benign non-parasitic hepatic cysts,hepatolithiasis/Caroli's disease or cirrhosis.We reported a case of primary SCC of the liver associated with multiple intrahepatic cholesterol gallstones.The patient underwent hepatectomy followed by radiotherapy,and has survived for over 19 mo without recurrence.展开更多
AIM:To assess the prevalence of human papilloma virus(HPV) in esophageal squamous cell carcinoma(ESCC) in the south-eastern region of Poland.METHODS:The study population consisted of 56 ESCC patients and 35 controls.T...AIM:To assess the prevalence of human papilloma virus(HPV) in esophageal squamous cell carcinoma(ESCC) in the south-eastern region of Poland.METHODS:The study population consisted of 56 ESCC patients and 35 controls.The controls were patients referred to our department due to other nonesophageal and non-oncological disorders with no gross or microscopic esophageal pathology as confirmed by endoscopy and histopathology.In the ESCC patients,samples were taken from normal mucosa(56 mucosa samples) and from the tumor(56 tumor samples).Tissue samples from the controls were taken from normal mucosa of the middle esophagus(35 control samples).Quantitative determination of DNA was carried out using a spectrophotometric method.Genomic DNA was isolated using the QIAamp DNA Midi Kit.HPV infection was identified following PCR amplification of the HPV gene sequence,using primers MY09 and MY11 complementary to the genome sequence of at least 33 types of HPV.The sequencing results were computationally analyzed using the basic local alignment search tool database.RESULTS:In tumor samples,HPV DNA was identified in 28 of 56 patients(50%).High risk HPV phenotypes(16 or/and 18) were found in 5 of 56 patients(8.9%),low risk in 19 of 56 patients(33.9%) and other types of HPV(37,81,97,CP6108) in 4 of 56 patients(7.1%).In mucosa samples,HPV DNA was isolated in 21 of 56 patients(37.5%).High risk HPV DNA was confirmed in 3 of 56 patients(5.3%),low risk HPV DNA in 12 of 56 patients(21.4%),and other types of HPV in 6 of 56 patients(10.7%).In control samples,HPV DNA was identified in 4 of 35 patients(11.4%) with no high risk HPV.The occurrence of HPV in ESCC patients was significantly higher than in the controls [28 of 56(50%) vs 4 of 35(11.4%),P < 0.001].In esophageal cancer patients,both in tumor and mucosa samples,the predominant HPV phenotypes were low risk HPV,isolated 4 times more frequently than high risk phenotypes [19 of 56(33.9%) vs 5 of 56(8.9%),P < 0.001].A higher prevalence of HPV was identified in female patients(71.4% vs 46.9%).Accordingly,the high risk phenotypes were isolated more frequently in female patients and this difference reached statistical significance [3 of 7(42.9%) vs 2 of 49(4.1%),P < 0.05].Of the pathological characteristics,only an infiltrative pattern of macroscopic tumor type significantly correlated with the presence of HPV DNA in ESCC samples [20 of 27(74.1%) vs 8 of 29(27.6%) for ulcerative or protruding macroscopic type,P < 0.05].The occurrence of total HPV DNA and both HPV high or low risk phenotypes did not significantly differ with regard to particular grades of cellular differentiation,phases in depth of tumor infiltration,grades of nodal involvement and stages of tumor progression.CONCLUSION:Low risk HPV phenotypes could be one of the co-activators or/and co-carcinogens in complex,progressive,multifactorial and multistep esophageal carcinogenesis.展开更多
目的研究抑癌基因(phosphatase and tens in homology deleted on chromo some ten,PTEN)在喉鳞状细胞癌、癌旁安全缘、声带息肉、转移淋巴结和无转移淋巴结中的表达,探讨其在喉鳞状细胞癌发生、发展和转移中的作用和意义,探讨喉鳞状细...目的研究抑癌基因(phosphatase and tens in homology deleted on chromo some ten,PTEN)在喉鳞状细胞癌、癌旁安全缘、声带息肉、转移淋巴结和无转移淋巴结中的表达,探讨其在喉鳞状细胞癌发生、发展和转移中的作用和意义,探讨喉鳞状细胞癌和转移淋巴结在分子水平的差异。方法采用免疫组织化学SP法检测了57例喉鳞状细胞癌患者的病理标本,随机抽取的27例癌旁安全切缘、22例声带息肉与57例标本中18例淋巴结转移阳性的喉状细胞鳞癌、转移淋巴结、无转移淋巴结中PTEN的表达进行检测和统计分析。结果PTEN在喉鳞状细胞癌、癌旁安全缘和声带息肉中的阳性率分别为89.5%(51/57)、88.9%(24/27)和95.5%(21/22),三者之间无显著性差异(P>0.05),但喉鳞状细胞癌、癌旁安全缘中PTEN的阳性表达程度较声带息肉中为低,有显著性差异(P<0.01);PTEN的阳性表达在不同性别、年龄、发病部位、分化、T分期、临床分期、淋巴结转移组间未见显著性差异(P>0.05)。无转移淋巴结中均未见PTEN的表达,18例转移淋巴结阳性患者喉鳞状细胞癌和转移淋巴结中PTEN的阳性率均为94.4%(17/18),但转移淋巴结中PTEN的表达强度较喉鳞状细胞癌高,差异有显著性(P<0.05)。结论在喉鳞状细胞癌发生的过程中,PTEN可能发生部分变异,属喉鳞状细胞癌癌变的早期分子事件,但与喉鳞状细胞癌的进展无关。喉鳞状细胞癌与转移淋巴结在分子水平存在差异。展开更多
Endoscopic mucosal resection is indicated in limited esophageal carcinomas with in?ltration of the mucosa. As submucosal cancer is combined with a rate of lymph node metastasis in up to 30% mucosectomy is not the proc...Endoscopic mucosal resection is indicated in limited esophageal carcinomas with in?ltration of the mucosa. As submucosal cancer is combined with a rate of lymph node metastasis in up to 30% mucosectomy is not the procedure of choice. The main techniques of endoscopic mucosal resection are the “suck and cut” technique using a cap on the endoscope or a ligation device to create a pseudocroup of the carcinoma. Submucosal injection of saline or other solutions is recommended prior to diathermic mucosectomy in order to reduce the risk of perforation or haemorrhage. The long term results of endoscopic mucosal resection show tumor speci?c 5 year survival rates of about 97% especially if the indication is restricted to m1 and m2 mucosal carcinomas.展开更多
文摘Primary squamous cell carcinoma(SCC) of the liver is rare and reported sporadically.Up to date,only 24 such cases have been reported in the literature.It is associated with hepatic teratoma,congenital cysts,solitary benign non-parasitic hepatic cysts,hepatolithiasis/Caroli's disease or cirrhosis.We reported a case of primary SCC of the liver associated with multiple intrahepatic cholesterol gallstones.The patient underwent hepatectomy followed by radiotherapy,and has survived for over 19 mo without recurrence.
基金Supported by Medical University of Lublin,Scientific Research Grant
文摘AIM:To assess the prevalence of human papilloma virus(HPV) in esophageal squamous cell carcinoma(ESCC) in the south-eastern region of Poland.METHODS:The study population consisted of 56 ESCC patients and 35 controls.The controls were patients referred to our department due to other nonesophageal and non-oncological disorders with no gross or microscopic esophageal pathology as confirmed by endoscopy and histopathology.In the ESCC patients,samples were taken from normal mucosa(56 mucosa samples) and from the tumor(56 tumor samples).Tissue samples from the controls were taken from normal mucosa of the middle esophagus(35 control samples).Quantitative determination of DNA was carried out using a spectrophotometric method.Genomic DNA was isolated using the QIAamp DNA Midi Kit.HPV infection was identified following PCR amplification of the HPV gene sequence,using primers MY09 and MY11 complementary to the genome sequence of at least 33 types of HPV.The sequencing results were computationally analyzed using the basic local alignment search tool database.RESULTS:In tumor samples,HPV DNA was identified in 28 of 56 patients(50%).High risk HPV phenotypes(16 or/and 18) were found in 5 of 56 patients(8.9%),low risk in 19 of 56 patients(33.9%) and other types of HPV(37,81,97,CP6108) in 4 of 56 patients(7.1%).In mucosa samples,HPV DNA was isolated in 21 of 56 patients(37.5%).High risk HPV DNA was confirmed in 3 of 56 patients(5.3%),low risk HPV DNA in 12 of 56 patients(21.4%),and other types of HPV in 6 of 56 patients(10.7%).In control samples,HPV DNA was identified in 4 of 35 patients(11.4%) with no high risk HPV.The occurrence of HPV in ESCC patients was significantly higher than in the controls [28 of 56(50%) vs 4 of 35(11.4%),P < 0.001].In esophageal cancer patients,both in tumor and mucosa samples,the predominant HPV phenotypes were low risk HPV,isolated 4 times more frequently than high risk phenotypes [19 of 56(33.9%) vs 5 of 56(8.9%),P < 0.001].A higher prevalence of HPV was identified in female patients(71.4% vs 46.9%).Accordingly,the high risk phenotypes were isolated more frequently in female patients and this difference reached statistical significance [3 of 7(42.9%) vs 2 of 49(4.1%),P < 0.05].Of the pathological characteristics,only an infiltrative pattern of macroscopic tumor type significantly correlated with the presence of HPV DNA in ESCC samples [20 of 27(74.1%) vs 8 of 29(27.6%) for ulcerative or protruding macroscopic type,P < 0.05].The occurrence of total HPV DNA and both HPV high or low risk phenotypes did not significantly differ with regard to particular grades of cellular differentiation,phases in depth of tumor infiltration,grades of nodal involvement and stages of tumor progression.CONCLUSION:Low risk HPV phenotypes could be one of the co-activators or/and co-carcinogens in complex,progressive,multifactorial and multistep esophageal carcinogenesis.
文摘目的研究抑癌基因(phosphatase and tens in homology deleted on chromo some ten,PTEN)在喉鳞状细胞癌、癌旁安全缘、声带息肉、转移淋巴结和无转移淋巴结中的表达,探讨其在喉鳞状细胞癌发生、发展和转移中的作用和意义,探讨喉鳞状细胞癌和转移淋巴结在分子水平的差异。方法采用免疫组织化学SP法检测了57例喉鳞状细胞癌患者的病理标本,随机抽取的27例癌旁安全切缘、22例声带息肉与57例标本中18例淋巴结转移阳性的喉状细胞鳞癌、转移淋巴结、无转移淋巴结中PTEN的表达进行检测和统计分析。结果PTEN在喉鳞状细胞癌、癌旁安全缘和声带息肉中的阳性率分别为89.5%(51/57)、88.9%(24/27)和95.5%(21/22),三者之间无显著性差异(P>0.05),但喉鳞状细胞癌、癌旁安全缘中PTEN的阳性表达程度较声带息肉中为低,有显著性差异(P<0.01);PTEN的阳性表达在不同性别、年龄、发病部位、分化、T分期、临床分期、淋巴结转移组间未见显著性差异(P>0.05)。无转移淋巴结中均未见PTEN的表达,18例转移淋巴结阳性患者喉鳞状细胞癌和转移淋巴结中PTEN的阳性率均为94.4%(17/18),但转移淋巴结中PTEN的表达强度较喉鳞状细胞癌高,差异有显著性(P<0.05)。结论在喉鳞状细胞癌发生的过程中,PTEN可能发生部分变异,属喉鳞状细胞癌癌变的早期分子事件,但与喉鳞状细胞癌的进展无关。喉鳞状细胞癌与转移淋巴结在分子水平存在差异。
文摘Endoscopic mucosal resection is indicated in limited esophageal carcinomas with in?ltration of the mucosa. As submucosal cancer is combined with a rate of lymph node metastasis in up to 30% mucosectomy is not the procedure of choice. The main techniques of endoscopic mucosal resection are the “suck and cut” technique using a cap on the endoscope or a ligation device to create a pseudocroup of the carcinoma. Submucosal injection of saline or other solutions is recommended prior to diathermic mucosectomy in order to reduce the risk of perforation or haemorrhage. The long term results of endoscopic mucosal resection show tumor speci?c 5 year survival rates of about 97% especially if the indication is restricted to m1 and m2 mucosal carcinomas.
