Optimization of Preparation of Oregano Oil Microspheres by Box-Behnken Response Surface Methodology

[Objectives]To optimize the formulation and preparation of oregano oil microspheres by Box-Behnken response surface methodology.[Methods]Chitosan was used as the carrier material to prepare oregano oil microspheres by emulsion crosslinking method.The encapsulation efficiency,drug loading and ID 50 were used as the evaluation indicators,and the comprehensive score(OD)obtained by"coefficient of variation-AHP comprehensive weighting method"was used as the final evaluation indicator.The formulation design and preparation process were optimized by single factor experiment and Box-Behnken response surface methodology,and the optimal process parameters were determined.[Results]The optimal formulation and preparation process parameters of oregano oil microspheres were as follows:the ratio of oregano oil to chitosan was 2∶1,the emulsifying speed of double emulsion was 200 r/min,the amount of emulsifier in the colostrum was 4%,and the volume of curing agent was 1.0 mL.The average encapsulation efficiency was 45.33%±1.32%,the average drug loading was 30.59%±2.45%,and the median diameter(ID 50)was 52.596μm±0.023%.[Conclusions]The encapsulation efficiency,drug loading and ID 50 of oregano oil chitosan microspheres prepared by emulsion crosslinking method met the requirements.The drug-loaded microsphere not only can be used as a preparation finished product for direct application,but also be used as a product intermediate to lay a foundation for the research and development of subsequent dosage forms.

Effects of Injection of Oregano Oil Submicron Emulsion on Lipopolysaccharide-induced Pneumonia in Rats

[Objectives]To explore the effects of injection of oregano oil submicron emulsion on lipopolysaccharide-induced pneumonia in rats.[Methods]Rats induced by lipopolysaccharide were used as animal models of acute pneumonia.The experiment was divided into blank group,model group,administration group and positive drug control group.The morphology of lung tissue and the changes of cells and inflammatory factors in each group were observed,and the anti-inflammatory effects of injection of oregano oil submicron emulsion.[Results]The injection of oregano oil submicron emulsion can improve the pathological injury of rat lung tissue,inhibit the release of IL-6,IL-10,TNF-αcytokines induced by lipopolysaccharide,and significantly reduce the value of CRP.[Conclusions]Oregano oil submicron emulsion has a certain therapeutic effect on lipopolysaccharide-induced pneumonia in rats,and its mechanism may be related to reducing the release of cytoinflammatory factor IL-6,IL-10,and TNF-αand alleviating the injury to tissues and organs.

Effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of weaned piglets

This experiment was conducted to investigate the effects of benzoic acid,Bacillus coagulans and oregano oil combined supplementation on growth performance,immune status and intestinal barrier integrity of piglets.In a 26-d experiment,25 piglets were randomly assigned to 5 treatments:1)a basal diet,negative control(NC),2)NC added with antibiotics,positive control(PC);3)NC added with benzoic acid at 3,000 g/t and Bacillus coagulans at 400 g/t(AB);4)NC added with benzoic acid at 3,000 g/t and o regano oil at 400 g/t(AO);5)NC added with 3,000 g/t benzoic acid and Bacillus coagulans at 400 g/t and oregano oil at 400 g/t(ABO);On d 27,all piglets were euthanized to obtain jejunal mucosa to measure immune status and intestinal barrier integrity.Results showed that pigs fed AB diet increased the final body weight and average daily body weight gain and decreased the ratio of feed to gain co mpared with NC group(P<0.05).Co mpared with NC group,AB,AO and ABO decreased serum tumor necrosis factor-a concentration and ABO decreased interleukin-1βconcentration in serum and jejunal mucosa(P<0.05).Compared with NC group,AB upregulated mRNA expressions of sodium-glucose cotransportel,claudin-1,occludin and mucin2 in jejunal mucosa and the populations of Bifidobacterium and Bacillus in cecal digesta(P<0.05).Compared with NC group,ABO increased jejunal mucosal occludin mRNA abundance and Bifidobacterium population in cecal digesta,and decreased Escherichia coli population in cecal digesta(P<0.05).Furthermore,AB and ABO increased Bacillus population in cecal digesta compared with PC group(P<0.05).These results indicated that dietary AB supplementation could improve growth performance and intestinal barrier integrity of piglets when fed antibiotic-free diets,which was possibly associated with the improvement of immune status and intestinal microflora.Dietary ABO supplementation is also beneficial to improve immune status and intestinal barrier integrity and micro flora of piglets.

Effect of Supplemental Oregano Essential Oils in Diets on Production Performance and Relatively Intestinal Parameters of Laying Hens

This study was designed to investigate the effects of dietary oregano essential oils on 150 30-week-old Hy-Line Layers’ productive performance, egg quality characteristics, cecal microbiota, digestive enzyme activities, mucosa structure of the duodenum and jejunum and glucose and small peptides transporters expression in the duodenum and jejunum. All hens were allocated into one of five different groups: negative control (NC;basal diet only), antibiotics control (CS;basal diet plus 100 mg/kg of 10% colistin sulfate) and treatments I, II, and III (basal diet supplemented with 50, 100 and 150 mg/kg oregano essential oils, respectively). The results showed that the laying rate, average egg weight, feed conversion ratio and the activities of amylase and trypsin were significantly improved by a diet supplemented with 100 mg/kg oregano essential oils (P 0.05), significantly increased duodenum villus-height-to-crypt-depth ratios (P 0.05).

Effect of Oregano Carvacrol on Growth Performance of Broiler

The product of plant essential oil, oregano carvacrol, was added in the diet of broiler, to study the effect of oregano carvacrol on growth performance of broilers. Using single factor test, 190 663 individuals of healthy AA broilers at 0 day old were randomly divided into two groups, eight repeats in control group and four repeats in experimental group. The broilers in control group were fed with basal diet （containing 10 mg/kg cnramycin and 60 mg/kg salinomycin sodium）, and those in experimental groups were fed with the basal diet adding with 100 mg/kg oregano carvacrol. Compared with control group, the daily gain of broilers in exper- imental group was increased noticeably by 3.41 g/broiler （P 〈 0. 05） ; the slaughter quality was increased remarkably by 143 g/broiler （P 〈 0.05 ） ; the survival rate was increased significantly by 3.01% （ P 〈 0.05 ） ; and the feed gain ratio was reduced markedly by 0.08 （ P 〈 0.05 ）. The paper provided a theoretical basis for application of oregano carvacrol in broiler production.

牛至精油对育肥猪生长性能、免疫性能和经济效益的影响

文章旨在探究牛至精油对育肥猪生长性能、免疫性能和经济效益的影响。试验将200头健康状态良好,体重在50kg左右的“杜×长×大”三元杂交育肥猪随机分为4组,每组5个重复,每个重复10头猪。对照组育肥猪饲喂基础日粮,3个处理组分别在基础日粮添加100、200、300mg/kg牛至精油,试验为期45d,其中预饲期3d,正试期42d。结果表明,(1)饲料添加100、200、300mg/kg牛至精油可显著增加育肥猪的ADG(P <0.05),显著降低F/G(P <0.05),添加200、300mg/kg牛至精油可以显著增加育肥猪的ADFI(P <0.05);(2)饲料添加200、300mg/kg牛至精油,可显著提高育肥猪血清中IgA、IgG的含量(P <0.05),显著降低TNF-α、IL-6和IL-10的含量(P <0.05);(3)饲料添加100、200、300mg/kg牛至精油可以显著降低育肥猪的增重成本(P <0.05),显著增加育肥猪养殖的毛利润(P <0.05)。且添加量在200mg/kg时效果最显著。

牛至精油的提取工艺优化及成分分析

研究旨在优化传统水蒸气蒸馏和同时蒸馏萃取2种方法提取牛至草中精油的工艺条件。通过单因素和正交实验优化提取参数,并对精油进行GC/MS分析。结果表明,水蒸气蒸馏法和同时蒸馏萃取法2种方法的最佳提取工艺为盐基度1%,提取时间度1.5 h,料液比1:6,在该条件下提取得率分别为0.486 g/100 g和0.608 g/100 g。水蒸气蒸馏法所得精油中烷类16种、烯类6种、醇类4种、酚类3种、酮类1种,含量占比分别为74.366%、6.263%、1.245%、12.684%、0.449%;同时蒸馏萃取法所得精油中烷类20种、烯类9种、醇类5种、酚类3种、酮类1种,含量分别占比60.127%、12.597%、2.795%、22.147%、0.936%。同时蒸馏萃取得到的精油组分种类和主要抑菌成分的含量明显多于水蒸气蒸馏法所得精油组分种类和含量。主要抑菌成分百里香酚、伞花烃、γ-松油烯、香芹酚和α-水芹烯在水蒸气蒸馏所得精油中的含量分别为8.492%、3.286%、2.551%、2.143%和0.206%,在同时蒸馏萃取所得精油中的含量分别为13.925%、5.218%、4.509%、4.14%和0.36%。采用本研究所得最佳工艺所得精油得率高,可为企业生产制备牛至精油提供参考。

代谢组学探究牛至精油对绵羊直肠粪便代谢物的影响

选择体重接近[(20.00±1.27)kg]的萨福克与小尾寒羊杂交公羔12只,随机分为两组,每组6只羊,分别为对照组和牛至精油组。对照组饲喂基础饲粮,牛至精油组饲喂基础饲粮+7 g·(d·sheep)^(-1)牛至精油。预试期10 d,正式期72 d,试验期结束后,采集直肠粪便进行液相色谱-串联质谱法(LC-MS/MS)非靶向代谢组学技术筛选差异代谢物。结果表明:1)两组试验羊的粪便中正、负离子模式共筛选出甲基-2,3-O-二烯丙基-4,6-O-亚苄基-alpha-D-吡喃甘露糖苷、二十二碳-4,7,10,13,16-五烯酸酯、甲基胍、腺嘌呤、18:1碳链溶血磷脂酰乙醇胺、N-乙酰-D-神经氨酸、环己烷羧酸、4-甲氧基苯乙酸、DL-多巴、胸腺嘧啶、去甲氧基姜黄素、5′-甲基硫代腺苷、间羟基苯甲酸、S-脱落酸、8-羟基鸟苷15种差异代谢物(|log2FC|>1,P<0.01,变量投影重要度>1),且与对照组相比,牛至精油组的差异代谢物全部上调。2)差异代谢物KEGG通路富集到萜类和类固醇的合成,微生物代谢,苯丙氨酸、酪氨酸和色氨酸的合成,半胱氨酸和蛋氨酸代谢,芳香族化合物的降解,代谢途径共6条通路(拓扑影响值>0.1),分别归属于脂质代谢、有机物分解与合成代谢和氨基酸代谢。这表明,牛至精油可能参与育肥羊脂质代谢、有机物代谢和氨基酸代谢,通过调节代谢途径发挥其在羊养殖中的健康维护作用。

牛至精油对肉牛生长性能、肉品质及经济效益的影响

试验旨在研究不同水平的牛至精油对肉牛生长性能、肉品质及经济效益的影响。选择体重相近、健康的西门塔尔肉牛52头,随机分成4组,每组13个重复,每个重复1头牛。对照组、EO10组、EO_(2)0组、EO40组肉牛分别饲喂添加0、10、20、40 g/(头·d)牛至精油的试验饲粮。预试期1 w,正式试验期90 d。结果显示,与对照组比,EO_(2)0组肉牛的平均日增重显著增加了25.84%(P<0.05),料重比显著降低了20.28%(P<0.05),EO_(2)0组肉牛的屠宰率和净肉率显著增加(P<0.05),EO_(2)0组和EO40组牛肉的pH值显著增加(P<0.05),肉色红度(a*)值显著提高(P<0.05),EO_(2)0组剪切力显著降低(P<0.05),EO_(2)0组和EO40组牛肉中粗蛋白、粗脂肪、顺式油酸含量显著增多(P<0.05),EO_(2)0组牛肉中亚油酸含量显著提高(P<0.05)。与对照组相比,EO10组、EO_(2)0组、EO40组肉牛的增重收益分别提高了11.24%、25.84%、15.73%,利润分别增加了23.28%、56.86%、1.50%。研究表明,在饲粮中添加20 g/(头·d)的牛至精油,肉牛的生长性能及养殖经济效益较好。

牛至精油缓解反刍动物瘤胃甲烷排放的研究进展

反刍动物温室气体的排放量占畜禽温室气体总排放量的80%,甲烷作为瘤胃微生物代谢的副产物,不仅对环境造成严重危害还降低了反刍动物的饲料能量利用率。植物提取物凭借其调节瘤胃微生物群落的功效在甲烷减排领域成为研究热点之一。牛至精油是从牛至中提取的一种含多种生物活性物质的植物提取物,以麝香草酚和香芹酚为主效因子,具有改善瘤胃内环境、改变瘤胃微生物组成、调控瘤胃发酵等生物学功能,在反刍动物养殖领域的应用前景十分广阔。关于牛至精油降低甲烷的作用效果已在多种动物上进行研究,结果表明牛至精油能通过调节瘤胃微生物及其代谢来降低瘤胃甲烷排放,但对其作用机制的研究还不够深入。作者以反刍动物为对象,对牛至精油通过调节瘤胃中细菌、古菌以及原虫的丰度和组成,调控瘤胃代谢,抑制二氧化碳还原途径和乙酸发酵途径,达到改善甲烷排放的作用机制进行综述,以期为牛至精油在饲料中的应用提供参考。

牛至精油对肉鸡生长性能、免疫功能和屠宰性能的影响

试验旨在研究牛至精油对沃德168肉鸡生长性能、免疫功能和屠宰性能的影响。试验选用1日龄沃德168肉鸡1000只,随机分为2组,每组5个重复,每个重复100只鸡。对照组饲喂基础饲粮,试验组在基础饲粮中添加300 g/t牛至精油。试验期45 d。结果显示,与对照组相比,试验组肉鸡料重比显著下降(P<0.05),半净膛率、全净膛率、胸肌率、腿肌率均显著提高(P<0.05)。与对照组相比,试验组肉鸡血清免疫球蛋白A(IgA)、免疫球蛋白M(IgM)含量和谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性及总抗氧化能力(T-AOC)显著或极显著提高(P<0.05或P<0.01),白细胞介素-1β(IL-1β)、干扰素-γ(IFN-γ)、丙二醛(MDA)含量显著或极显著降低(P<0.05或P<0.01)。与对照组相比,试验组肉鸡脾脏器官指数显著提高(P<0.05),法氏囊器官指数极显著提高(P<0.01)。研究表明,饲粮中添加300 g/t牛至精油可以提高肉鸡生长性能、免疫能力、抗氧化性能和屠宰性能。

复方牛至油纳米乳的制备及其对母猪子宫内膜炎的治疗效果观察

试验旨在制备复方牛至油纳米乳,探讨复方牛至油纳米乳对猪子宫内膜炎的治疗效果。试验以牛至油、醋酸氯己定为药物原料,选取合适的表面活性剂、助表面活性剂和油相,通过伪三元相图法筛选制备纳米乳的配方,并对试验制备的纳米乳进行质量和稳定性的初步考察,对临床型母猪子宫内膜炎进行分组治疗。结果显示,以RH-40、吐温-80为表面活性剂且质量比为1∶1制得的复方牛至油纳米乳外观澄清透明,粒径为圆形球状液滴,分布均匀,无团聚现象、无分层、无浑浊、无沉淀析出。临床型母猪子宫内膜炎治疗试验结果显示,复方牛至油纳米乳Ⅰ组、复方牛至油纳米乳Ⅱ组母猪子宫异常分泌物分泌持续时间明显缩短,子宫内膜炎的治愈率显著高于复方牛至油纳米乳Ⅲ组、对照组Ⅰ组、对照组Ⅱ组(P<0.05),再次发情率、受胎率均高于其他治疗组。研究表明,复方牛至油纳米乳具有较好的稳定性,对临床型母猪子宫内膜炎具有明显的治疗效果。

三种植物精油和纯露在沐浴露和洗手液中的抑菌抗氧化性能研究

以牛至、迷迭香和艾草的精油和纯露为抑菌抗氧化成分,添加到沐浴露和洗手液中,通过滤纸片法和抑菌率法与添加传统化学抑菌抗氧化剂的产品进行抑菌性能的比较。采用DPPH法、水杨酸法、普鲁士蓝法体外抗氧化实验,比较各产品的抗氧化能力。结果表明:牛至纯露加质量分数在5%~15%,迷迭香纯露加质量分数在1%~15%,艾草纯露加质量分数在1%~15%时对绿脓假单胞菌、大肠杆菌、白色念珠菌三种菌为高度敏感。抑菌率实验和现场手消毒实验结果表明添加植物精油和纯露的洗手液及沐浴露产品和添加化学抑菌抗氧化剂的产品对三种供试菌种均有显著的抑菌作用。抗氧化性测定实验结果表明添加醋酸氯己定的沐浴露产品清除DPPH·能力和还原力最强,添加植物精油和纯露的沐浴露产品具有与化学抑菌抗氧化剂沐浴露产品相当的抗氧化能力。添加0.4%的牛至精油或迷迭香精油的洗手液产品抗氧化性强于添加化学抑菌抗氧化剂的洗手液产品。该研究结果可为企业添加牛至、迷迭香及艾草的精油和纯露生产绿色日化产品提供重要的参考依据,为减少日化产品中化学抑菌抗氧化剂的毒性、致癌等危害、发挥天然抑菌抗氧化剂高效抑菌和低毒性等优势提供支持。

丁酸钠和牛至油对断奶仔猪生长性能、肠道菌群和组织形态学结构的影响

为研究丁酸钠、牛至油及其复配使用对断奶仔猪生长性能、肠道菌群和组织形态学结构的影响,试验将200头(28±2)日龄、体重(8.03±0.5)kg的断奶“杜×长×大”三元杂交仔猪(公猪去势,公母比例1∶1)随机分为5组:对照A组饲喂基础日粮;对照B组(抗生素组:基础日粮1000kg+15%金霉素500g+杆菌肽锌250g),试验C组(基础日粮1000kg+2kg丁酸钠),试验D组(基础日粮1000kg+1kg牛至油),试验E组(基础日粮1000kg+1kg丁酸钠+0.5kg牛至油),预饲期7d,正试期28d。结果表明,在断奶仔猪基础日粮中添加丁酸钠与牛至油可显著提高仔猪日增重、日采食量、空肠和回肠绒毛高度(P <0.05),增加盲肠和回肠中乳酸杆菌菌群数及VH/CD值(P <0.05),显著降低隐窝深度(P <0.05)、料肉比以及盲肠和回肠大肠杆菌的菌群数(P <0.05)。总之,丁酸钠、牛至油及其联合使用可调节肠道菌群平衡,改善肠道健康状况,显著提高断奶仔猪的生长性能。

复配精油对粮油中霉菌防治的增效作用及机理研究

开发新型天然防霉剂控制粮食霉变是保障粮食质量安全的重要途径之一。为研究复配植物精油对粮油储藏过程中常见霉菌赭曲霉(Aspergillus ochrator)、黄曲霉(Aspergillus flavus)和黑曲霉(Aspergillus niger)的防霉效果,挑选活性较强的植物精油进行复配并对联合防霉效果进行评价。通过复配植物精油对霉菌孢子萌发、菌丝干重和细胞完整性的影响,对其防霉机理进行初步探究。结果显示,牛至精油对赭曲霉和黑曲霉的防霉效果最好,抑菌圈直径分别为(27.83±0.58)、(15.33±0.29)mm,肉桂醛对黄曲霉的防霉效果最好,抑菌圈直径为(18.50±0.87)mm;山苍子精油与牛至精油、肉桂醛与牛至精油复配体积比为2∶8时对3种霉菌的防霉效果较优;通过对部分抑菌浓度指数判读,2组植物精油复配对黑曲霉和赭曲霉的防治效果为协同作用,对黄曲霉的防治效果为相加作用;山苍子精油与牛至精油按体积比2∶8复配可抑制霉菌孢子萌发和菌丝生长、破坏细胞的完整性、改变孢子和菌丝结构;山苍子精油和牛至精油按体积比2∶8复配施用于含黄曲霉的玉米上,可有效降低玉米中黄曲霉毒素B 1和赭曲霉毒素的含量。

牛至精油-聚乙烯醇/壳聚糖复合膜的制备及对鸡肉保鲜效果的影响

目的研究牛至精油(Oregano essential oil,OEO)的添加对聚乙烯醇(Polyvinyl alcohol,PVA)/壳聚糖(Chitosan,CS)复合膜理化性质及功能特性的影响。方法以PVA、CS为基材,以OEO为抗菌剂,采用流延法制备复合膜,并测定复合膜的物理性能、抗氧化性和抑菌性,并评估其在鸡肉保鲜中的应用效果。结果OEO的加入使得复合膜的厚度、水分含量、水接触角、拉伸强度、断裂伸长率显著提高,水蒸气透过率显著降低。此外,OEO增强了复合膜的抗氧化性和抑菌性,PVA/CS-OEO4的DPPH自由基清除率达到73.80%,负载OEO的PVA/CS复合膜能够抑制大肠杆菌和金黄色葡萄球菌的生长。将复合膜应用于4℃下的鸡肉保鲜,与对照组相比,负载OEO的复合膜能显著抑制鸡肉的微生物生长和氧化变质,延缓了鸡肉pH和感官品质的变化,其中PVA/CS-OEO4的效果最明显。结论OEO的添加能够提高PVA/CS的力学性能、水蒸气阻隔性、抗氧化和抑菌性;在4℃条件下可将鸡肉的货架寿命延长至10 d。

牛至油在鸡生产上的应用研究进展

牛至油是从牛至的全草中提取出来的具有提高动物机体抗氧化、抗寄生虫、抗炎、抗菌、免疫力和减少应激反应能力的挥发性食用植物精油,是农业农村部批准使用的一种纯正的中药药物添加剂。在鸡的饲料中添加牛至油可以提高鸡的生产性能,改善鸡肉的品质,提高鸡的免疫力和抗应激能力。该文章综述了牛至油在鸡生产中的应用进展情况,为牛至油在鸡生产实际中的应用提供参考。

不同水平牛至油对肉牛生长性能、肉品质及经济效益的影响

为分析基础日粮中不同水平牛至油对肉牛生长性能、肉品质及经济效益的影响。试验选取120头10月龄、体重相近(341.25±3.74kg)的健康肉牛,随机分成4组,每组3个重复,每个重复10头。对照组饲喂基础日粮,其余3组分别用基础日粮+100、200、300 g/t牛至油饲喂。试验为期60 d,试验期间所有肉牛自由采食、自由饮水。结果显示:(1)生长性能方面,与对照组相比,试验组干物质采食量和平均日增重均有提高,其中200 g/t牛至油组显著提高14.32%(P<0.05)、13.71%(P<0.05);300 g/t牛至油组显著提高11.44%(P<0.05)、11.29%(P<0.05)。(2)肉品质方面,与对照组相比,100、200、300 g/t牛至油组失水率、蒸煮损失、剪切力均显著降低(P<0.05),且100、200 g/t牛至油组失水率显著高于300 g/t牛至油组(P<0.05)。(3)经济效益方面,与对照组相比,100、200、300 g/t牛至油组饲料成本均显著提高(P<0.05),且100 g/t牛至油组饲料成本显著低于200、300 g/t牛至油组(P<0.05)。与对照组相比,200、300 g/t牛至油组饲料消耗量、肉牛增重、增重效益、毛利润均显著提高(P<0.05)。其中200 g/t牛至油组的毛利润最高,达到648.13元/头。综上所述,在肉牛养殖中,牛至油具有提高生长性能、肉品质及经济效益的效果。其中,牛至油添加水平为200 g/t时,肉牛生长性能和经济效益指标最佳。

牛至精油对腌腊肉感官及脂肪氧化的影响

为探究牛至精油对传统腌腊肉制品感官品质及脂肪氧化的影响,当牛至精油添加量为0,0.5,1.0,1.5 g/kg时,分别测定腌腊肉的感官、色值和过氧化值在储存期间的变化,结果显示:由于牛至精油具有独特的香气,将其添加到腌腊肉中可以提高腌腊肉的感官品质;牛至精油还可以减缓L^(*),a^(*),b^(*)值的变化,降低储存期间的色差ΔE;添加牛至精油后,腌腊肉的过氧化值降低,并且其在储存期间的上升趋势随牛至精油添加量的增加而减缓,说明牛至精油可以提高腌腊肉的抗氧化能力。研究结果表明:当牛至精油添加量为1.0 g/kg时,腌腊肉的抗氧化能力最强并能保持较高的感官品质。

Microencapsulation of Essential Oils by Interfacial Polimerization Using Polyurea as a Wall Material

An essential oil is the volatile lipophilic component extracted from plants. Microencapsulation systems protect the essential oil from degradation and evaporation, and, at the same time, allow a sustained release. This work analyses and characterizes the oregano and sage essential oil microcapsules prepared by interfacial polymerization technique, using polyurea as wall material. Several instrumental techniques are used: optical microscopy, size particle, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), Termo gravimetric analysis (TGA), spectrophotometry, antimicrobial test and chromatography. Results show that oregano and sage oil have antimicrobial properties, and their microencapsulation allows knowing that these properties remain inside the microcapsules.