The aqueous two-phase system(ATPS)is an all-aqueous system fabricated from two immiscible aqueous phases.It is spontaneously assembled through physical liquid-liquid phase separation(LLPS)and can create suitable templ...The aqueous two-phase system(ATPS)is an all-aqueous system fabricated from two immiscible aqueous phases.It is spontaneously assembled through physical liquid-liquid phase separation(LLPS)and can create suitable templates like the multicompartment of the intracellular environment.Delicate structures containing multiple compartments make it possible to endow materials with advanced functions.Due to the properties of ATPSs,ATPS-based drug delivery systems exhibit excellent biocompatibility,extraordinary loading efficiency,and intelligently controlled content release,which are particularly advantageous for delivering drugs in vivo.Therefore,we will systematically review and evaluate ATPSs as an ideal drug delivery system.Based on the basic mechanisms and influencing factors in forming ATPSs,the transformation of ATPSs into valuable biomaterials is described.Afterward,we concentrate on the most recent cutting-edge research on ATPS-based delivery systems.Finally,the potential for further collaborations between ATPS-based drug-carrying biomaterials and disease diagnosis and treatment is also explored.展开更多
Successful regenerative medicine strategies of xenogeneic extracellular matrix need a synergistic balance among inflammation,fibrosis,and remodeling process.Adaptive macrophage subsets have been identified to modulate...Successful regenerative medicine strategies of xenogeneic extracellular matrix need a synergistic balance among inflammation,fibrosis,and remodeling process.Adaptive macrophage subsets have been identified to modulate inflammation and orchestrate the repair of neighboring parenchymal tissues.This study fabricated PPARγ-primed CD68+CD206+M2 phenotype(M2γ),and firstly verified their anti-inflammatory and tissue-regenerating roles in xenogeneic bioengineered organ regeneration.Our results showed that Th1-type CD3^(+)CD8^(+)T cell response to xenogeneic-dentin matrix-based bioengineered root complex(xeno-complex)was significantly inhibited by M2γmacrophage in vitro.PPARγactivation also timely recruited CD68^(+)CD206^(+)tissue macrophage polarization to xeno-complex in vivo.These subsets alleviated proinflammatory cytokines(TNF-α,IFN-γ)at the inflammation site and decreased CD3^(+)CD8^(+)T lymphocytes in the periphery system.When translated to an orthotopic nonhuman primate model,PPARγ-primed M2 macrophages immunosuppressed IL-1β,IL-6,TNF-α,MMPs to enable xeno-complex to effectively escape immune-mediated rejection and initiate graft-host synergistic integrity.These collective activities promoted the differentiation of odontoblast-like and periodontal-like cells to guide pulp-dentin and cementum-PDLs-bone regeneration and rescued partially injured odontogenesis such as DSPP and periostin expression.Finally,the regenerated root showed structure-biomechanical and functional equivalency to the native tooth.The timely conversion of M1-to-M2 macrophage mainly orchestrated odontogenesis,fibrogenesis,and osteogenesis,which represents a potential modulator for intact parenchymal-stromal tissue regeneration of targeted organs.展开更多
Three-dimensional(3D)bioprinting,an additive manufacturing based technique of biomaterials fabrication,is an innovative and auspicious strategy in medical and pharmaceutical fields.The ability of producing regenerativ...Three-dimensional(3D)bioprinting,an additive manufacturing based technique of biomaterials fabrication,is an innovative and auspicious strategy in medical and pharmaceutical fields.The ability of producing regenerative tissues and organs has made this technology a pioneer to the creation of artificial multi-cellular tissues/organs.A broad variety of biomaterials is currently being utilized in 3D bioprinting as well as multiple techniques employed by researchers.In this review,we demonstrate the most common and novel biomaterials in 3D bioprinting technology further with introducing the related techniques that are commonly taking into account by researchers.In addition,an attempt has been accomplished to hand over the most relevant application of 3D bioprinting techniques such as tissue regeneration,cancer investigations,etc.by presenting the most important works.The main aim of this review paper is to emphasis on strengths and limitations of existence biomaterials and 3D bioprinting techniques in order to carry out a comparison through them.展开更多
Plant somatic cells have the capability to switch their cell fates from differentiated to undifferen-tiated status under proper culture conditions,which is designated as totipotency.As a result,plant cells can easily ...Plant somatic cells have the capability to switch their cell fates from differentiated to undifferen-tiated status under proper culture conditions,which is designated as totipotency.As a result,plant cells can easily regenerate new tissues or organs from a wide variety of explants.However,the mechanism by which plant cells have such remarkable regeneration ability is still largely unknown.In this study,we used a set of meristem-specific marker genes to analyze the patterns of stem cell differentiation in the processes of somatic embryogenesis as well as shoot or root organogenesis in vitro.Our studies furnish preliminary and important information on the patterns of the de novo stem cell differentiation during various types of in vitro organogenesis.展开更多
基金This study was supported by National Natural Science Foundation of China Youth Science Fund Project(Grant number 82001107)the Applied Basic Research Project of Sichuan province(Grant number 2022NSFSC1345,China).
文摘The aqueous two-phase system(ATPS)is an all-aqueous system fabricated from two immiscible aqueous phases.It is spontaneously assembled through physical liquid-liquid phase separation(LLPS)and can create suitable templates like the multicompartment of the intracellular environment.Delicate structures containing multiple compartments make it possible to endow materials with advanced functions.Due to the properties of ATPSs,ATPS-based drug delivery systems exhibit excellent biocompatibility,extraordinary loading efficiency,and intelligently controlled content release,which are particularly advantageous for delivering drugs in vivo.Therefore,we will systematically review and evaluate ATPSs as an ideal drug delivery system.Based on the basic mechanisms and influencing factors in forming ATPSs,the transformation of ATPSs into valuable biomaterials is described.Afterward,we concentrate on the most recent cutting-edge research on ATPS-based delivery systems.Finally,the potential for further collaborations between ATPS-based drug-carrying biomaterials and disease diagnosis and treatment is also explored.
基金This work was supported by the National Key Research and Development Program of China(Nos.2017YFA0104800),Nature Science Foundation of China(31771062,31971281,81901001),Key Research and Development Program of Sichuan Province(2017SZ0031).
文摘Successful regenerative medicine strategies of xenogeneic extracellular matrix need a synergistic balance among inflammation,fibrosis,and remodeling process.Adaptive macrophage subsets have been identified to modulate inflammation and orchestrate the repair of neighboring parenchymal tissues.This study fabricated PPARγ-primed CD68+CD206+M2 phenotype(M2γ),and firstly verified their anti-inflammatory and tissue-regenerating roles in xenogeneic bioengineered organ regeneration.Our results showed that Th1-type CD3^(+)CD8^(+)T cell response to xenogeneic-dentin matrix-based bioengineered root complex(xeno-complex)was significantly inhibited by M2γmacrophage in vitro.PPARγactivation also timely recruited CD68^(+)CD206^(+)tissue macrophage polarization to xeno-complex in vivo.These subsets alleviated proinflammatory cytokines(TNF-α,IFN-γ)at the inflammation site and decreased CD3^(+)CD8^(+)T lymphocytes in the periphery system.When translated to an orthotopic nonhuman primate model,PPARγ-primed M2 macrophages immunosuppressed IL-1β,IL-6,TNF-α,MMPs to enable xeno-complex to effectively escape immune-mediated rejection and initiate graft-host synergistic integrity.These collective activities promoted the differentiation of odontoblast-like and periodontal-like cells to guide pulp-dentin and cementum-PDLs-bone regeneration and rescued partially injured odontogenesis such as DSPP and periostin expression.Finally,the regenerated root showed structure-biomechanical and functional equivalency to the native tooth.The timely conversion of M1-to-M2 macrophage mainly orchestrated odontogenesis,fibrogenesis,and osteogenesis,which represents a potential modulator for intact parenchymal-stromal tissue regeneration of targeted organs.
文摘Three-dimensional(3D)bioprinting,an additive manufacturing based technique of biomaterials fabrication,is an innovative and auspicious strategy in medical and pharmaceutical fields.The ability of producing regenerative tissues and organs has made this technology a pioneer to the creation of artificial multi-cellular tissues/organs.A broad variety of biomaterials is currently being utilized in 3D bioprinting as well as multiple techniques employed by researchers.In this review,we demonstrate the most common and novel biomaterials in 3D bioprinting technology further with introducing the related techniques that are commonly taking into account by researchers.In addition,an attempt has been accomplished to hand over the most relevant application of 3D bioprinting techniques such as tissue regeneration,cancer investigations,etc.by presenting the most important works.The main aim of this review paper is to emphasis on strengths and limitations of existence biomaterials and 3D bioprinting techniques in order to carry out a comparison through them.
基金supported by grants from the Ministry of Science and Technology of China(No.2007CB948200)the National Natural Science Foundation(NNSF)of China(Grant Nos.90917015 and 30770217).
文摘Plant somatic cells have the capability to switch their cell fates from differentiated to undifferen-tiated status under proper culture conditions,which is designated as totipotency.As a result,plant cells can easily regenerate new tissues or organs from a wide variety of explants.However,the mechanism by which plant cells have such remarkable regeneration ability is still largely unknown.In this study,we used a set of meristem-specific marker genes to analyze the patterns of stem cell differentiation in the processes of somatic embryogenesis as well as shoot or root organogenesis in vitro.Our studies furnish preliminary and important information on the patterns of the de novo stem cell differentiation during various types of in vitro organogenesis.